Planarian EST Database

DrC_00328

Cluster detail
GO Category : Not Available
Consensus Sequence :
>DrC_00328 TTCGGCACGAGGACAACAAAGATCAGTGGAGTTCTCGTCAATATTTCGAAATTACGATAA CTTACGAATAGGTTTATTGGAAGCTATGCCTGTTATGGAAACTCGTGTATTATCAGGCAC TCTTACTGGGAACAATGAATTGACTGTAACAGAAAAATCCAATAATATGCCTGGTCAACT GAATGGATATGGTTGCGAGATTATTAGTGATGTGCCGGAAAATAAAACCAATAATGCTGG TCAATTACTCGATTTATTATCATTAACTGATCCAGTTTCTTCAACTAATAACCAATCGAC CGATTTAAATTTAAATGGAACGGCCATTTCTAACAATTCAACAGGAAATATTTTTGATCT ACTCGGAGATCTTTCGTTGAATTCTGAAATGCCACCCATATCCAATCATAAAGCTCCATC AACCAATGCGACACCTCAAATAAGTGATACTAAACCAGCAATATCGTCAATCATAGTATA CGAAAGCAGTCATGGCCTCAAATTGACATTTTCATTTGAACGAATTTCCAACAGTCAAAG TACAGTGACCCTTCATGCAATTAATAATGGCAATCAATTGATTGATCAATTTCTATTCCA AGCAGCAGTACCGAAATCATTTCAGTTGGTGCTTCTTGCACCTTCATCCGATAACCTGAT GCCATTTGGTGGCAGCTTAACCCAGTTGCTCAAATTATCAAATCCGAATAAACAGCCTTG GAAAATGAAAATACGCTTGCAGTATCGATTAAACGGAGAAGTCAAATCAGTACAGCACCA GTTGGACACATTCCCATCTGACTTGTGGACCTAAACCGTAAACGTTTCAATGGCTTCTTA TTTTTATCGGGGTTGAAGCTATTTTATTGATTTATTTTATCGATCAAGTGCTGTGAATCA ATATTACACATTGTTTTTTCATTTAAACCCCTTATTGATATTTTTTTGATTTGTCGTTAT ATGCGTTGTTTCCCTGATATCATTATTGCACGAATAAAAATTGACTTTG

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00328
         (1009 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P22892|AP1G1_MOUSE  Adapter-related protein complex 1 gam...   128   2e-29
sp|O43747|AP1G1_HUMAN  Adapter-related protein complex 1 gam...   126   9e-29
sp|O88512|AP1G2_MOUSE  Adapter-related protein complex 1 gam...    92   2e-18
sp|O75843|AP1G2_HUMAN  Adapter-related protein complex 1 gam...    85   3e-16
sp|Q9NZ52|GGA3_HUMAN  ADP-ribosylation factor binding protei...    55   3e-07
sp|P38817|GGA2_YEAST  ADP-ribosylation factor binding protei...    54   9e-07
sp|Q8R0H9|GGA1_MOUSE  ADP-ribosylation factor binding protei...    53   1e-06
sp|Q99128|ADG_USTMA  Gamma-adaptin (Golgi adaptor HA1/AP1 ad...    53   2e-06
sp|Q9UJY5|GGA1_HUMAN  ADP-ribosylation factor binding protei...    53   2e-06
sp|Q8BMI3|GGA3_MOUSE  ADP-ribosylation factor binding protei...    51   5e-06

>sp|P22892|AP1G1_MOUSE Adapter-related protein complex 1 gamma 1 subunit (Gamma-adaptin)
            (Adaptor protein complex AP-1 gamma-1 subunit) (Golgi
            adaptor HA1/AP1 adaptin gamma-1 subunit) (Clathrin
            assembly protein complex 1 gamma-1 large chain)
          Length = 822

 Score =  128 bits (322), Expect = 2e-29
 Identities = 98/284 (34%), Positives = 144/284 (50%), Gaps = 19/284 (6%)
 Frame = +2

Query: 14   QQRSVEFSSIFRNYDNLRIGLLEAMPVMETRVLSGTLTGNNELTVTEKSNNMPGQLNGYG 193
            QQR+VE++++F+ YD++R  LLE MPVME      T  G +E+  T      P  L    
Sbjct: 560  QQRAVEYNALFKKYDHMRSALLERMPVME----KVTTNGPSEIVQTNGETE-PAPLE--- 611

Query: 194  CEIISDVPEN---KTNNAGQLLDLLSLTD--PVSSTNNQSTDLNLNGTAISNNSTGNIFD 358
                +  P +    T+ A  LLDLL   D  PV  T   S   +  G          + D
Sbjct: 612  ----TKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPASAGG---------ELLD 658

Query: 359  LLGDLSLN---------SEMPPISNHK--APSTNATPQISDTKPAISSIIVYESSHGLKL 505
            LLGD++L          + +P IS         ++ P  +D  P I SI  Y S +GLK+
Sbjct: 659  LLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLFNDIAPGIPSITAY-SKNGLKI 717

Query: 506  TFSFERISNSQST--VTLHAINNGNQLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFG-GS 676
             F+FER + + S   +T+ A N+    +  F+FQAAVPK+FQL LL+PSS  +  F  G+
Sbjct: 718  EFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVVPAFNTGT 777

Query: 677  LTQLLKLSNPNKQPWKMKIRLQYRLNGEVKSVQHQLDTFPSDLW 808
            +TQ++K+ NP KQ  +M+I+L Y   G       +++ FP   W
Sbjct: 778  ITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSW 821

>sp|O43747|AP1G1_HUMAN Adapter-related protein complex 1 gamma 1 subunit (Gamma-adaptin)
            (Adaptor protein complex AP-1 gamma-1 subunit) (Golgi
            adaptor HA1/AP1 adaptin gamma-1 subunit) (Clathrin
            assembly protein complex 1 gamma-1 large chain)
          Length = 822

 Score =  126 bits (317), Expect = 9e-29
 Identities = 97/284 (34%), Positives = 146/284 (51%), Gaps = 19/284 (6%)
 Frame = +2

Query: 14   QQRSVEFSSIFRNYDNLRIGLLEAMPVMETRVLSGTLTGNNELTVTEKSNNMPGQLNGYG 193
            QQR+VE++++F+ YD++R  LLE MPVME      T  G  E+  T      P  L    
Sbjct: 560  QQRAVEYNALFKKYDHMRSALLERMPVME----KVTTNGPTEIVQTNGETE-PAPLE--- 611

Query: 194  CEIISDVPEN---KTNNAGQLLDLLSLTD--PVSSTNNQSTDLNLNGTAISNNSTGNIFD 358
                +  P +    T+ A  LLDLL   D  PV  T           T+  +++ G + D
Sbjct: 612  ----TKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAP---------TSKPSSAGGELLD 658

Query: 359  LLGDLSLN---------SEMPPIS--NHKAPSTNATPQISDTKPAISSIIVYESSHGLKL 505
            LLGD++L          + +P IS  +      ++ P  +D    I SI  Y S +GLK+
Sbjct: 659  LLGDINLTGAPAAAPAPASVPQISQPHFLLDGLSSQPLFNDIAAGIPSITAY-SKNGLKI 717

Query: 506  TFSFERISNSQST--VTLHAINNGNQLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFG-GS 676
             F+FER + + S   +T+ A N+    +  F+FQAAVPK+FQL LL+PSS  +  F  G+
Sbjct: 718  EFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGT 777

Query: 677  LTQLLKLSNPNKQPWKMKIRLQYRLNGEVKSVQHQLDTFPSDLW 808
            +TQ++K+ NP KQ  +M+I+L Y   G       +++ FP   W
Sbjct: 778  ITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSW 821

>sp|O88512|AP1G2_MOUSE Adapter-related protein complex 1 gamma 2 subunit (Gamma2-adaptin)
            (Adaptor protein complex AP-1 gamma-2 subunit) (G2ad)
          Length = 791

 Score = 92.4 bits (228), Expect = 2e-18
 Identities = 82/272 (30%), Positives = 126/272 (46%), Gaps = 7/272 (2%)
 Frame = +2

Query: 14   QQRSVEFSSIFRNYDNLRIGLLEAMPVMETRVLSGTLTGNNELTVTEKSNNMPGQLNGYG 193
            QQR+VE++++F+ YD++R  +LE MP++E         G+  +    K      Q     
Sbjct: 561  QQRAVEYNTLFQKYDHMRAAILEKMPLVER--------GDPHV----KEGGKEKQTEAQP 608

Query: 194  CEIISDVPENKTNNAGQLLDLLSL----TDPVSSTNNQSTDLNLNGTAISNNSTGNIFDL 361
             E+ +  P      A +LLDLL L    ++P+SS + Q              S G     
Sbjct: 609  LEVTAPAPTEP--QATKLLDLLDLLGDTSEPLSSGHAQHLPPQ-------TPSPGEALIH 659

Query: 362  LGDLSLNSEMPPISNHKAPSTNATPQISDTKPAISSIIVYESSHGLKLTFSFERISNSQS 541
            L DL      P      AP              I S+ V+E   GL+L  SF R   + +
Sbjct: 660  LLDLPCTPPPP------AP--------------IPSVRVFERE-GLQLDLSFMRPLETPA 698

Query: 542  T--VTLHAINNGNQLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFGG-SLTQLLKLSNPNK 712
               VT    N+  + +  F+ QAAVPKSFQL L APS + +   GG  +TQ+ ++ NPN+
Sbjct: 699  LLLVTATTTNSSKEDVTHFVCQAAVPKSFQLQLQAPSGNTIPAQGGLPITQVFRILNPNQ 758

Query: 713  QPWKMKIRLQYRLNGEVKSVQHQLDTFPSDLW 808
             P ++K+RL Y  +G+      ++D  P + W
Sbjct: 759  APLRLKLRLTYNHSGQPVQEIFEVDNLPVETW 790

>sp|O75843|AP1G2_HUMAN Adapter-related protein complex 1 gamma 2 subunit (Gamma2-adaptin)
            (Adaptor protein complex AP-1 gamma-2 subunit) (G2ad)
          Length = 785

 Score = 85.1 bits (209), Expect = 3e-16
 Identities = 77/268 (28%), Positives = 118/268 (44%), Gaps = 3/268 (1%)
 Frame = +2

Query: 14   QQRSVEFSSIFRNYDNLRIGLLEAMPVMETRVLSGTLTGNNELTVTEKSNNMPGQLNGYG 193
            QQR+VE+ ++FR YD++R  +LE MP++E            +     K +    QL+   
Sbjct: 561  QQRAVEYDTLFRKYDHMRAAILEKMPLVE--------RDGPQADEEAKESKEAAQLS--- 609

Query: 194  CEIISDVPENKTNNAGQLLDLLSLTDPVSSTNNQSTDLNLNGTAISNNSTGNIFDLLGDL 373
                   P      A QLLDLL L D  S        L+        +  G +  LL   
Sbjct: 610  ----EAAPVPTEPQASQLLDLLDLLDGASGDVQHPPHLD-------PSPGGALVHLL--- 655

Query: 374  SLNSEMPPISNHKAPSTNATPQISDTKPAISSIIVYESSHGLKLTFSFERISNSQS--TV 547
                ++P +    AP              I  + V+E   G++L  SF R   + +   +
Sbjct: 656  ----DLPCVPPPPAP--------------IPDLKVFE-REGVQLNLSFIRPPENPALLLI 696

Query: 548  TLHAINNGNQLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFGG-SLTQLLKLSNPNKQPWK 724
            T+ A N     +  F+ QAAVPKS QL L APS + +   GG  +TQL ++ NPNK P +
Sbjct: 697  TITATNFSEGDVTHFICQAAVPKSLQLQLQAPSGNTVPARGGLPITQLFRILNPNKAPLR 756

Query: 725  MKIRLQYRLNGEVKSVQHQLDTFPSDLW 808
            +K+RL Y    +      +++  P + W
Sbjct: 757  LKLRLTYDHFHQSVQEIFEVNNLPVESW 784

>sp|Q9NZ52|GGA3_HUMAN ADP-ribosylation factor binding protein GGA3 (Golgi-localized, gamma
            ear-containing, ARF-binding protein 3)
          Length = 723

 Score = 55.1 bits (131), Expect = 3e-07
 Identities = 39/162 (24%), Positives = 72/162 (44%), Gaps = 9/162 (5%)
 Frame = +2

Query: 338  STGNIFDLLGDLSLNSEMPPISNHKAPSTNATPQISDTKPAISSIIVYESSHGLKLTFSF 517
            STG    L   LS  S+  P    +    +    +   KP+ +  +     +G ++ F F
Sbjct: 554  STGPGSPLFQPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHF 613

Query: 518  ERISNSQS----TVTLHAINNGNQLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFG----- 670
             +           V +  +N     +   + QAAVPKS ++ L  PS   L PF      
Sbjct: 614  AKECPPGRPDVLVVVVSMLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPP 673

Query: 671  GSLTQLLKLSNPNKQPWKMKIRLQYRLNGEVKSVQHQLDTFP 796
             ++TQ++ L+NP K+  +++ +L + L  ++ +   ++D FP
Sbjct: 674  AAITQVMLLANPLKEKVRLRYKLTFALGEQLSTEVGEVDQFP 715

>sp|P38817|GGA2_YEAST ADP-ribosylation factor binding protein GGA2 (Golgi-localized,
           gamma ear-containing, ARF-binding protein 2)
          Length = 585

 Score = 53.5 bits (127), Expect = 9e-07
 Identities = 57/189 (30%), Positives = 80/189 (42%), Gaps = 2/189 (1%)
 Frame = +2

Query: 215 PENKTNNAGQLLDLLSLTDPVSSTNNQSTDLNLNGTAISNNSTGNIFDLLGDLSLNSEMP 394
           P   TNN+   LDLL L+ P S TN+Q+            NS+G  FDLL   +      
Sbjct: 421 PVTTTNNSNNTLDLLGLSTPQSPTNSQAV-----------NSSG--FDLLMGFN------ 461

Query: 395 PISNHKAPSTNATPQISDTKPAISSIIVYESSHGLKLTFSFERISNSQSTVTLHAINNGN 574
                  P+T  T     T PA + +     S  LK+ F   R SNS   +     N  +
Sbjct: 462 -------PTTGTT-----TAPARTLV---NQSPNLKIEFEISRESNSVIRIKSFFTNLSS 506

Query: 575 QLIDQFLFQAAVPKSFQLVLLAPSSDNLMPFGG--SLTQLLKLSNPNKQPWKMKIRLQYR 748
             I   +F  AVPKS  L  L P S N M       ++Q   + N    P K  ++++++
Sbjct: 507 SPISNLVFLLAVPKSMSL-KLQPQSSNFMIGNAKDGISQEGTIENAPANPSK-ALKVKWK 564

Query: 749 LNGEVKSVQ 775
           +N  V S Q
Sbjct: 565 VNYSVNSTQ 573

>sp|Q8R0H9|GGA1_MOUSE ADP-ribosylation factor binding protein GGA1 (Golgi-localized,
           gamma ear-containing, ARF-binding protein 1)
           (Gamma-adaptin-related protein 1)
          Length = 635

 Score = 53.1 bits (126), Expect = 1e-06
 Identities = 37/135 (27%), Positives = 66/135 (48%), Gaps = 10/135 (7%)
 Frame = +2

Query: 422 TNATPQISDTKPA-ISSIIVYESSHGLKLTFSFERIS----NSQSTVTLHAINNGNQLID 586
           T+ T  +   KP+ I  + VY+  HG ++ F F R      +    V +  ++   Q I 
Sbjct: 494 TSITVPLESIKPSSILPVTVYDQ-HGFRVLFHFARDPLPGRSDVLVVVVSMLSTAPQPIR 552

Query: 587 QFLFQAAVPKSFQLVLLAPSSDNLMPFG-----GSLTQLLKLSNPNKQPWKMKIRLQYRL 751
             +FQ+AVPK  ++ L  PS   L  F       ++TQ+L L+NP K+  +++ +L + +
Sbjct: 553 NIVFQSAVPKVMKVRLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLIFTM 612

Query: 752 NGEVKSVQHQLDTFP 796
             +  +    +D FP
Sbjct: 613 GDQTYNEMGDVDQFP 627

>sp|Q99128|ADG_USTMA Gamma-adaptin (Golgi adaptor HA1/AP1 adaptin gamma subunit) (Clathrin
            assembly protein complex 1 gamma large chain) (Gamma-ADA)
          Length = 853

 Score = 52.8 bits (125), Expect = 2e-06
 Identities = 67/289 (23%), Positives = 118/289 (40%), Gaps = 31/289 (10%)
 Frame = +2

Query: 14   QQRSVEFSSIFRNYDNLRIGLLEAMPVMETR-VLSGTLTGNNELTVTEKSNNMPGQLNGY 190
            QQRSVEF+++ +  D +R G+LE+MP  E +  + GT++    +  T    +    L G 
Sbjct: 566  QQRSVEFATLLKRSD-IRQGVLESMPPPEIKQTVLGTVSEAKPVGSTRSDKDALLDLMGD 624

Query: 191  GCEIIS-------DVPENKTNNAGQLLDLLSLTDPVSSTNNQSTDLNLNGTAISNNSTGN 349
               + S       + P   T  +    DLL+            +       + +     +
Sbjct: 625  EMPVTSGGGTGADNAPSGATQQSTH--DLLADIFGGGDMGGMPSAAPAASASAAQKPKSS 682

Query: 350  IFDLLGDLSLNSEMPPISNHKAP----------------------STNATPQISDTKPAI 463
            + D+LG     +  P  +  +AP                      S+ +TP  +      
Sbjct: 683  VNDILGLFGDGASAPAAAAQQAPTPAPAATSSYGGLDLLGGLGASSSASTPAATPASTVA 742

Query: 464  SSIIVYESSHGLKLTFSFERISNSQSTVTLHA-INNGNQLIDQFLFQAAVPKSFQLVLLA 640
             S  VY + HGL +T +          V + A   +    I    FQAAVPK+ +L + A
Sbjct: 743  KSHTVY-TKHGLTITLTPTTNPARPEIVHITARFTSATSAISNINFQAAVPKTHKLQMQA 801

Query: 641  PSSDNLMPFGGSLTQLLKLSNPNKQPWKMKIRLQYRLNGEVKSVQHQLD 787
             S+  + P   + TQ L++  P     ++++R+ ++++G   SVQ Q D
Sbjct: 802  ISNSTVHP-DSTETQPLRVMVPPGAAVRLRLRIAFQVDGH--SVQDQTD 847

>sp|Q9UJY5|GGA1_HUMAN ADP-ribosylation factor binding protein GGA1 (Golgi-localized,
           gamma ear-containing, ARF-binding protein 1)
           (Gamma-adaptin related protein 1)
          Length = 639

 Score = 52.8 bits (125), Expect = 2e-06
 Identities = 36/132 (27%), Positives = 64/132 (48%), Gaps = 10/132 (7%)
 Frame = +2

Query: 431 TPQISDTKPA-ISSIIVYESSHGLKLTFSFERIS----NSQSTVTLHAINNGNQLIDQFL 595
           T  +   KP+ I  + VY+  HG ++ F F R      +    V +  ++   Q I   +
Sbjct: 501 TVPLESIKPSNILPVTVYDQ-HGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIV 559

Query: 596 FQAAVPKSFQLVLLAPSSDNLMPFG-----GSLTQLLKLSNPNKQPWKMKIRLQYRLNGE 760
           FQ+AVPK  ++ L  PS   L  F       ++TQ+L L+NP K+  +++ +L + +  +
Sbjct: 560 FQSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQ 619

Query: 761 VKSVQHQLDTFP 796
             +    +D FP
Sbjct: 620 TYNEMGDVDQFP 631

>sp|Q8BMI3|GGA3_MOUSE ADP-ribosylation factor binding protein GGA3 (Golgi-localized,
           gamma ear-containing, ARF-binding protein 3)
          Length = 718

 Score = 51.2 bits (121), Expect = 5e-06
 Identities = 31/128 (24%), Positives = 61/128 (47%), Gaps = 9/128 (7%)
 Frame = +2

Query: 440 ISDTKPAISSIIVYESSHGLKLTFSFERISNSQS----TVTLHAINNGNQLIDQFLFQAA 607
           +   KP+ +  +     +G ++ F F +           V +  +N     +   + QAA
Sbjct: 583 LESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSMLNTAPLPVKSIVLQAA 642

Query: 608 VPKSFQLVLLAPSSDNLMPFG-----GSLTQLLKLSNPNKQPWKMKIRLQYRLNGEVKSV 772
           VPKS ++ L  PS   L PF       ++TQ++ L+NP K+  +++ +L + L  ++ + 
Sbjct: 643 VPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPMKEKVRLRYKLTFALGEQLSTE 702

Query: 773 QHQLDTFP 796
             ++D FP
Sbjct: 703 LGEVDQFP 710

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 117,447,143
Number of Sequences: 369166
Number of extensions: 2433017
Number of successful extensions: 5792
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5708
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10718264480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00328

Planaria EST Database

DrC_00328

BLASTX 2.2.13 [Nov-27-2005]

Cluster detail