Planaria EST Database

DrC_00323

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00323
         (865 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q24799|MYPH_ECHGR  Myophilin                                   140   3e-33
sp|Q99439|CNN2_HUMAN  Calponin-2 (Calponin H2, smooth muscle...   139   1e-32
sp|Q08093|CNN2_MOUSE  Calponin-2 (Calponin H2, smooth muscle...   137   3e-32
sp|Q08094|CNN2_PIG  Calponin-2 (Calponin H2, smooth muscle) ...   137   5e-32
sp|P26932|CNN1_CHICK  Calponin-1 (Calponin, smooth muscle)        131   3e-30
sp|P37397|CNN3_RAT  Calponin-3 (Calponin, acidic isoform) (C...   130   5e-30
sp|P19966|TAGL_CHICK  Transgelin (Smooth muscle protein 22-a...   129   1e-29
sp|Q15417|CNN3_HUMAN  Calponin-3 (Calponin, acidic isoform)       128   2e-29
sp|P14318|MP20_DROME  Muscle-specific protein 20                  128   2e-29
sp|Q9DAW9|CNN3_MOUSE  Calponin-3 (Calponin, acidic isoform)       128   2e-29

>sp|Q24799|MYPH_ECHGR Myophilin
          Length = 190

 Score =  140 bits (354), Expect = 3e-33
 Identities = 82/203 (40%), Positives = 119/203 (58%), Gaps = 2/203 (0%)
 Frame = +3

Query: 87  MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHH-LKNGQIL 263
           M+N     G+   + ++L  K D D E EA+EWI  LTG  + L R  +    LK+G +L
Sbjct: 1   MSNVPPPSGLSYQVKKKLEGKRDKDQENEALEWIEALTG--LKLDRSKLYEDILKDGTVL 58

Query: 264 VKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQT 443
            KL+N I       P   ++          T PFK MENI  FL A + +GVP   LFQT
Sbjct: 59  CKLMNSIK------PGCIKKINE-----NATMPFKIMENISAFLEAMKGYGVPVADLFQT 107

Query: 444 VDLFESRNMAQVLNSILQLGTECQRN-GFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINL 620
           VDLFE +++AQV  ++  LG  CQ +  +SGP+ GP+  + N R+F+++QLR G+ +++L
Sbjct: 108 VDLFEKKDIAQVTRTLFALGRTCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVVSL 167

Query: 621 QSGTNKFASQSGMSFGGVRHIAD 689
           Q G+NK ASQ+G++ G  R I D
Sbjct: 168 QYGSNKGASQAGINMGKQRMIMD 190

>sp|Q99439|CNN2_HUMAN Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
          Length = 309

 Score =  139 bits (349), Expect = 1e-32
 Identities = 97/260 (37%), Positives = 135/260 (51%), Gaps = 4/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  RL SKYDP  E E   WI  LTG +I     + Q  LK+G IL  L
Sbjct: 7   NKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIG---PDFQKGLKDGTILCTL 63

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           +N +       P S  +       N     + Q+EN+  F++A  S+G+    LF+  DL
Sbjct: 64  MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111

Query: 453 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FES NM QV  S+L L  + +  G  SG   G + +    R F D  ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 803
           TNK ASQSGM ++G  RH+ D +   L       I LQMGTNKCASQ GM+  G  RHI 
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIY 231

Query: 804 DIKCESN-CPEAASIINLQY 860
           D K  ++ C  ++  + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251

>sp|Q08093|CNN2_MOUSE Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
          Length = 305

 Score =  137 bits (346), Expect = 3e-32
 Identities = 97/260 (37%), Positives = 135/260 (51%), Gaps = 4/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  RL SKYDP  E E   WI  LTG +I     + Q  LK+G IL  L
Sbjct: 7   NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGVILCTL 63

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           +N +       P S  +       N     + Q+EN+  F++A  S+G+    LF+  DL
Sbjct: 64  MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111

Query: 453 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FES NM QV  S+L L  + +  G  SG   G + +    R F D  ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 803
           TNK ASQSGM ++G  RH+ D +   L       I LQMGTNKCASQ GM+  G  RHI 
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHCTISLQMGTNKCASQVGMTAPGTRRHIY 231

Query: 804 DIKCESN-CPEAASIINLQY 860
           D K  ++ C  ++  + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251

>sp|Q08094|CNN2_PIG Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
          Length = 296

 Score =  137 bits (344), Expect = 5e-32
 Identities = 95/260 (36%), Positives = 134/260 (51%), Gaps = 4/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  RL SKYDP  E E   WI  LTG +I     + Q  LK+G IL  L
Sbjct: 7   NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGIILCTL 63

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           +N +       P S  +       N     + Q+EN+  F++A  S+G+    LF+  DL
Sbjct: 64  MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111

Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FES NM QV  S+L L  + +  G    +  G + +    R F D  ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSDVDIGVKYSEKQQRNFDDATMKAGQCVIGLQMG 171

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 803
           TNK ASQSGM ++G  RH+ D +   L       I LQMGTNKCASQ GM+  G  RHI 
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIY 231

Query: 804 DIKCESN-CPEAASIINLQY 860
           D K  ++ C  ++  + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251

>sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle)
          Length = 292

 Score =  131 bits (329), Expect = 3e-30
 Identities = 94/264 (35%), Positives = 137/264 (51%), Gaps = 7/264 (2%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           NR   +G+   +  +L  KYDP +E++   WI   TG  I    +N    LK+G IL +L
Sbjct: 7   NRGPAYGLSAEVKNKLAQKYDPQTERQLRVWIEGATGRRIG---DNFMDGLKDGVILCEL 63

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           IN +       P S ++   P +       + ++ENI  FLRA + +GV    +F+  DL
Sbjct: 64  INKLQ------PGSVQKVNDPVQN------WHKLENIGNFLRAIKHYGVKPHDIFEANDL 111

Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGT 632
           FE+ N  QV ++++ L ++ +  G +  L G +      R+F  E+LR G  II LQ GT
Sbjct: 112 FENTNHTQVQSTLIALASQAKTKGNNVGL-GVKYAEKQQRRFQPEKLREGRNIIGLQMGT 170

Query: 633 NKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIAD 806
           NKFASQ GM ++G  RH+ D +        +  I LQMGTNK ASQ GM+  G  R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230

Query: 807 -----IKCESNCPEAASIINLQYG 863
                 +C++N      II LQ G
Sbjct: 231 PSLGMERCDTN------IIGLQMG 248

>sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (Calponin, non-muscle
           isoform)
          Length = 330

 Score =  130 bits (327), Expect = 5e-30
 Identities = 90/260 (34%), Positives = 137/260 (52%), Gaps = 3/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  ++ SKYD  +E++   WI ++TG  I     N Q  LK+G IL +L
Sbjct: 5   NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGMGIGT---NFQLGLKDGIILCEL 61

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           IN +       P S ++       N  +  + Q+ENI  F++A +++G+    +F+  DL
Sbjct: 62  INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109

Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FE+ NM QV  +++ L    +  GF   +  G +      R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 803
           TNK ASQ+GM ++G  RH+ D +        +  I LQMGTNK ASQ GMS  G  R I 
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMSAPGTRRDIY 229

Query: 804 DIKCESNCPEAASIINLQYG 863
           D K     P   S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248

>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
          Length = 200

 Score =  129 bits (324), Expect = 1e-29
 Identities = 81/206 (39%), Positives = 117/206 (56%), Gaps = 7/206 (3%)
 Frame = +3

Query: 87  MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNI---PLGRENVQHHLKNGQ 257
           MAN+   +G+   +  ++  KYD + E   +EWI    GS++     GR   Q  LKNG 
Sbjct: 1   MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGI 60

Query: 258 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 437
           +L +L+N +Y      PD ++  K P    TM   FKQME I  FL+AAE +GV +  +F
Sbjct: 61  VLSQLVNSLY------PDGSKPVKIPDSPPTMV--FKQMEQIAQFLKAAEDYGVVKTDMF 112

Query: 438 QTVDLFESRNMAQVLNSILQLG----TECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGE 605
           QTVDLFE+++MA V  +++ LG    T+   +    P    +    + R+FS+ QL+ G+
Sbjct: 113 QTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGK 172

Query: 606 GIINLQSGTNKFASQSGMSFGGVRHI 683
            II LQ GTNK ASQ+GMS+G  R I
Sbjct: 173 NIIGLQMGTNKGASQAGMSYGRPRQI 198

>sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform)
          Length = 329

 Score =  128 bits (322), Expect = 2e-29
 Identities = 89/260 (34%), Positives = 138/260 (53%), Gaps = 3/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  ++ SKYD  +E++   WI ++TG +I     N Q  LK+G IL +L
Sbjct: 5   NKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIG---PNFQLGLKDGIILCEL 61

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           IN +       P S ++       N  +  + Q+ENI  F++A +++G+    +F+  DL
Sbjct: 62  INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109

Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FE+ NM QV  +++ L    +  GF   +  G +      R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGM-SIGAVRHIA 803
           TNK ASQ+GM ++G  RH+ D +        +  I LQMGTNK ASQ GM + G  R I 
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229

Query: 804 DIKCESNCPEAASIINLQYG 863
           D K     P   S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248

>sp|P14318|MP20_DROME Muscle-specific protein 20
          Length = 184

 Score =  128 bits (322), Expect = 2e-29
 Identities = 72/191 (37%), Positives = 113/191 (59%), Gaps = 1/191 (0%)
 Frame = +3

Query: 114 IDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKLINVIYER 293
           ++ A+  ++ SK +P+ ++EA EWI  +     P G ++ +  LK+GQ+L KLINV+   
Sbjct: 3   LERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL--- 58

Query: 294 TPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDLFESRNMA 473
           +PN            + N+    FK MENI  F +A + +GVP   +FQTVDL+E +++A
Sbjct: 59  SPNAVP---------KVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIA 109

Query: 474 QVLNSILQLGTECQRNG-FSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGTNKFASQ 650
            V N+I  LG    ++  F GP  GP+P     R F++EQL+ G+ I+ LQ+G+NK A+Q
Sbjct: 110 NVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQ 169

Query: 651 SGMSFGGVRHI 683
           +G + G  R I
Sbjct: 170 AGQNLGAGRKI 180

>sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform)
          Length = 330

 Score =  128 bits (321), Expect = 2e-29
 Identities = 89/260 (34%), Positives = 137/260 (52%), Gaps = 3/260 (1%)
 Frame = +3

Query: 93  NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
           N+   +G+   +  ++ SKYD  +E++   WI ++TG  I     N Q  LK+G IL +L
Sbjct: 5   NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGLGIGT---NFQLGLKDGIILCEL 61

Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
           IN +       P S ++       N  +  + Q+ENI  F++A +++G+    +F+  DL
Sbjct: 62  INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109

Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
           FE+ NM QV  +++ L    +  GF   +  G +      R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169

Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGM-SIGAVRHIA 803
           TNK ASQ+GM ++G  RH+ D +        +  I LQMGTNK ASQ GM + G  R I 
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229

Query: 804 DIKCESNCPEAASIINLQYG 863
           D K     P   S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,532,607
Number of Sequences: 369166
Number of extensions: 2287271
Number of successful extensions: 5392
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5058
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5271
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00323

  1. Dr_sW_008_J02
  2. Dr_sW_016_G24