Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_J02
(806 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24799|MYPH_ECHGR Myophilin 140 3e-33
sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-a... 129 9e-30
sp|P14318|MP20_DROME Muscle-specific protein 20 128 2e-29
sp|Q99439|CNN2_HUMAN Calponin-2 (Calponin H2, smooth muscle... 128 2e-29
sp|P37802|TAGL2_HUMAN Transgelin-2 (SM22-alpha homolog) 127 5e-29
sp|Q08093|CNN2_MOUSE Calponin-2 (Calponin H2, smooth muscle... 127 5e-29
sp|Q08094|CNN2_PIG Calponin-2 (Calponin H2, smooth muscle) ... 126 8e-29
sp|Q9WVA4|TAGL2_MOUSE Transgelin-2 125 1e-28
sp|Q5E9F5|TAGL2_BOVIN Transgelin-2 125 1e-28
sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle) 124 2e-28
>sp|Q24799|MYPH_ECHGR Myophilin
Length = 190
Score = 140 bits (354), Expect = 3e-33
Identities = 82/203 (40%), Positives = 119/203 (58%), Gaps = 2/203 (0%)
Frame = +3
Query: 87 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHH-LKNGQIL 263
M+N G+ + ++L K D D E EA+EWI LTG + L R + LK+G +L
Sbjct: 1 MSNVPPPSGLSYQVKKKLEGKRDKDQENEALEWIEALTG--LKLDRSKLYEDILKDGTVL 58
Query: 264 VKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQT 443
KL+N I P ++ T PFK MENI FL A + +GVP LFQT
Sbjct: 59 CKLMNSIK------PGCIKKINE-----NATMPFKIMENISAFLEAMKGYGVPVADLFQT 107
Query: 444 VDLFESRNMAQVLNSILQLGTECQRN-GFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINL 620
VDLFE +++AQV ++ LG CQ + +SGP+ GP+ + N R+F+++QLR G+ +++L
Sbjct: 108 VDLFEKKDIAQVTRTLFALGRTCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVVSL 167
Query: 621 QSGTNKFASQSGMSFGGVRHIAD 689
Q G+NK ASQ+G++ G R I D
Sbjct: 168 QYGSNKGASQAGINMGKQRMIMD 190
>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
Length = 200
Score = 129 bits (324), Expect = 9e-30
Identities = 81/206 (39%), Positives = 117/206 (56%), Gaps = 7/206 (3%)
Frame = +3
Query: 87 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNI---PLGRENVQHHLKNGQ 257
MAN+ +G+ + ++ KYD + E +EWI GS++ GR Q LKNG
Sbjct: 1 MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGI 60
Query: 258 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 437
+L +L+N +Y PD ++ K P TM FKQME I FL+AAE +GV + +F
Sbjct: 61 VLSQLVNSLY------PDGSKPVKIPDSPPTMV--FKQMEQIAQFLKAAEDYGVVKTDMF 112
Query: 438 QTVDLFESRNMAQVLNSILQLG----TECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGE 605
QTVDLFE+++MA V +++ LG T+ + P + + R+FS+ QL+ G+
Sbjct: 113 QTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGK 172
Query: 606 GIINLQSGTNKFASQSGMSFGGVRHI 683
II LQ GTNK ASQ+GMS+G R I
Sbjct: 173 NIIGLQMGTNKGASQAGMSYGRPRQI 198
>sp|P14318|MP20_DROME Muscle-specific protein 20
Length = 184
Score = 128 bits (322), Expect = 2e-29
Identities = 72/191 (37%), Positives = 113/191 (59%), Gaps = 1/191 (0%)
Frame = +3
Query: 114 IDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKLINVIYER 293
++ A+ ++ SK +P+ ++EA EWI + P G ++ + LK+GQ+L KLINV+
Sbjct: 3 LERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL--- 58
Query: 294 TPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDLFESRNMA 473
+PN + N+ FK MENI F +A + +GVP +FQTVDL+E +++A
Sbjct: 59 SPNAVP---------KVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIA 109
Query: 474 QVLNSILQLGTECQRNG-FSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGTNKFASQ 650
V N+I LG ++ F GP GP+P R F++EQL+ G+ I+ LQ+G+NK A+Q
Sbjct: 110 NVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQ 169
Query: 651 SGMSFGGVRHI 683
+G + G R I
Sbjct: 170 AGQNLGAGRKI 180
>sp|Q99439|CNN2_HUMAN Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 309
Score = 128 bits (321), Expect = 2e-29
Identities = 87/227 (38%), Positives = 118/227 (51%), Gaps = 2/227 (0%)
Frame = +3
Query: 93 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIG---PDFQKGLKDGTILCTL 63
Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 453 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
FES NM QV S+L L + + G SG G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171
Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQ 767
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQ 218
>sp|P37802|TAGL2_HUMAN Transgelin-2 (SM22-alpha homolog)
Length = 199
Score = 127 bits (318), Expect = 5e-29
Identities = 80/203 (39%), Positives = 117/203 (57%), Gaps = 7/203 (3%)
Frame = +3
Query: 87 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPL---GRENVQHHLKNGQ 257
MANR +G+ + +++ +YD D EQ I+WI ++ GREN Q+ LK+G
Sbjct: 1 MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGT 60
Query: 258 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 437
+L +LIN +Y P+ K + T FKQME I FL+AAE +G+ +F
Sbjct: 61 VLCELINALY------PEGQAPVK---KIQASTMAFKQMEQISQFLQAAERYGINTTDIF 111
Query: 438 QTVDLFESRNMAQVLNSILQLG--TECQRNG-FSG-PLCGPRPTSSNFRQFSDEQLRMGE 605
QTVDL+E +NMA V +++ LG + +G FSG P P+ + N R FSD QL+ G+
Sbjct: 112 QTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGK 171
Query: 606 GIINLQSGTNKFASQSGMSFGGV 674
+I LQ GTN+ ASQ+GM+ G+
Sbjct: 172 NVIGLQMGTNRGASQAGMTGYGM 194
>sp|Q08093|CNN2_MOUSE Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 305
Score = 127 bits (318), Expect = 5e-29
Identities = 87/227 (38%), Positives = 118/227 (51%), Gaps = 2/227 (0%)
Frame = +3
Query: 93 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGVILCTL 63
Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 453 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
FES NM QV S+L L + + G SG G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171
Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQ 767
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHCTISLQMGTNKCASQ 218
>sp|Q08094|CNN2_PIG Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 296
Score = 126 bits (316), Expect = 8e-29
Identities = 85/227 (37%), Positives = 117/227 (51%), Gaps = 2/227 (0%)
Frame = +3
Query: 93 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGIILCTL 63
Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 629
FES NM QV S+L L + + G + G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSDVDIGVKYSEKQQRNFDDATMKAGQCVIGLQMG 171
Query: 630 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQ 767
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQ 218
>sp|Q9WVA4|TAGL2_MOUSE Transgelin-2
Length = 212
Score = 125 bits (315), Expect = 1e-28
Identities = 79/199 (39%), Positives = 114/199 (57%), Gaps = 7/199 (3%)
Frame = +3
Query: 87 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPL---GRENVQHHLKNGQ 257
MANR +G+ + +++ +YD D EQ I+WI ++ GREN Q LK+G
Sbjct: 1 MANRGPSYGLSREVQQKIEKQYDADLEQILIQWITTQCREDVGQPQPGRENFQKWLKDGT 60
Query: 258 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 437
+L KLIN +Y P+ K + + FKQME I FL+AAE +G+ +F
Sbjct: 61 VLCKLINSLY------PEGQAPVK---KIQASSMAFKQMEQISQFLQAAERYGINTTDIF 111
Query: 438 QTVDLFESRNMAQVLNSILQLG--TECQRNG-FSG-PLCGPRPTSSNFRQFSDEQLRMGE 605
QTVDL+E +NMA V +++ LG + +G FSG P P+ + N R FSD QL+ G+
Sbjct: 112 QTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGK 171
Query: 606 GIINLQSGTNKFASQSGMS 662
+I LQ GTN+ ASQ+GM+
Sbjct: 172 NVIGLQMGTNRGASQAGMT 190
>sp|Q5E9F5|TAGL2_BOVIN Transgelin-2
Length = 199
Score = 125 bits (314), Expect = 1e-28
Identities = 80/203 (39%), Positives = 117/203 (57%), Gaps = 7/203 (3%)
Frame = +3
Query: 87 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPL---GRENVQHHLKNGQ 257
MANR +G+ + +++ +YD D EQ I+WI ++ GREN Q+ LK+G
Sbjct: 1 MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGT 60
Query: 258 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 437
+L +LIN +Y P+ K + T FKQME I FL+AAE +G+ +F
Sbjct: 61 VLCELINGLY------PEGQAPVK---KIQASTMAFKQMEQISQFLQAAERYGINTTDIF 111
Query: 438 QTVDLFESRNMAQVLNSILQLG--TECQRNG-FSG-PLCGPRPTSSNFRQFSDEQLRMGE 605
QTVDL+E +NMA V +++ LG + +G FSG P P+ + N R FSD QL+ G+
Sbjct: 112 QTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRYFSDNQLQEGK 171
Query: 606 GIINLQSGTNKFASQSGMSFGGV 674
+I LQ GTN+ ASQ+GM+ G+
Sbjct: 172 NVIGLQMGTNRGASQAGMTGYGM 194
>sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle)
Length = 292
Score = 124 bits (312), Expect = 2e-28
Identities = 82/226 (36%), Positives = 120/226 (53%), Gaps = 1/226 (0%)
Frame = +3
Query: 93 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 272
NR +G+ + +L KYDP +E++ WI TG I +N LK+G IL +L
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDPQTERQLRVWIEGATGRRIG---DNFMDGLKDGVILCEL 63
Query: 273 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 452
IN + P S ++ P + + ++ENI FLRA + +GV +F+ DL
Sbjct: 64 INKLQ------PGSVQKVNDPVQN------WHKLENIGNFLRAIKHYGVKPHDIFEANDL 111
Query: 453 FESRNMAQVLNSILQLGTECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGT 632
FE+ N QV ++++ L ++ + G + L G + R+F E+LR G II LQ GT
Sbjct: 112 FENTNHTQVQSTLIALASQAKTKGNNVGL-GVKYAEKQQRRFQPEKLREGRNIIGLQMGT 170
Query: 633 NKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQ 767
NKFASQ GM ++G RH+ D + + I LQMGTNK ASQ
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQ 216
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,293,450
Number of Sequences: 369166
Number of extensions: 2101427
Number of successful extensions: 4937
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4641
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4833
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)