Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_G24
(850 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24799|MYPH_ECHGR Myophilin 140 3e-33
sp|Q99439|CNN2_HUMAN Calponin-2 (Calponin H2, smooth muscle... 139 1e-32
sp|Q08093|CNN2_MOUSE Calponin-2 (Calponin H2, smooth muscle... 137 3e-32
sp|Q08094|CNN2_PIG Calponin-2 (Calponin H2, smooth muscle) ... 137 5e-32
sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle) 131 3e-30
sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (C... 130 5e-30
sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-a... 129 1e-29
sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform) 128 2e-29
sp|P14318|MP20_DROME Muscle-specific protein 20 128 2e-29
sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform) 128 2e-29
>sp|Q24799|MYPH_ECHGR Myophilin
Length = 190
Score = 140 bits (354), Expect = 3e-33
Identities = 82/203 (40%), Positives = 119/203 (58%), Gaps = 2/203 (0%)
Frame = +3
Query: 72 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHH-LKNGQIL 248
M+N G+ + ++L K D D E EA+EWI LTG + L R + LK+G +L
Sbjct: 1 MSNVPPPSGLSYQVKKKLEGKRDKDQENEALEWIEALTG--LKLDRSKLYEDILKDGTVL 58
Query: 249 VKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQT 428
KL+N I P ++ T PFK MENI FL A + +GVP LFQT
Sbjct: 59 CKLMNSIK------PGCIKKINE-----NATMPFKIMENISAFLEAMKGYGVPVADLFQT 107
Query: 429 VDLFESRNMAQVLNSILQLGTECQRN-GFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINL 605
VDLFE +++AQV ++ LG CQ + +SGP+ GP+ + N R+F+++QLR G+ +++L
Sbjct: 108 VDLFEKKDIAQVTRTLFALGRTCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVVSL 167
Query: 606 QSGTNKFASQSGMSFGGVRHIAD 674
Q G+NK ASQ+G++ G R I D
Sbjct: 168 QYGSNKGASQAGINMGKQRMIMD 190
>sp|Q99439|CNN2_HUMAN Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 309
Score = 139 bits (349), Expect = 1e-32
Identities = 97/260 (37%), Positives = 135/260 (51%), Gaps = 4/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIG---PDFQKGLKDGTILCTL 63
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 438 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FES NM QV S+L L + + G SG G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 788
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ GM+ G RHI
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIY 231
Query: 789 DIKCESN-CPEAASIINLQY 845
D K ++ C ++ + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251
>sp|Q08093|CNN2_MOUSE Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 305
Score = 137 bits (346), Expect = 3e-32
Identities = 97/260 (37%), Positives = 135/260 (51%), Gaps = 4/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGVILCTL 63
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 438 FESRNMAQVLNSILQLGTECQRNGF-SGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FES NM QV S+L L + + G SG G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMG 171
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 788
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ GM+ G RHI
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHCTISLQMGTNKCASQVGMTAPGTRRHIY 231
Query: 789 DIKCESN-CPEAASIINLQY 845
D K ++ C ++ + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251
>sp|Q08094|CNN2_PIG Calponin-2 (Calponin H2, smooth muscle) (Neutral calponin)
Length = 296
Score = 137 bits (344), Expect = 5e-32
Identities = 95/260 (36%), Positives = 134/260 (51%), Gaps = 4/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + RL SKYDP E E WI LTG +I + Q LK+G IL L
Sbjct: 7 NKGPSYGLSAEVKNRLLSKYDPQKEAELRSWIEGLTGLSIG---PDFQKGLKDGIILCTL 63
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
+N + P S + N + Q+EN+ F++A S+G+ LF+ DL
Sbjct: 64 MNKLQ------PGSVPK------INRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDL 111
Query: 438 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FES NM QV S+L L + + G + G + + R F D ++ G+ +I LQ G
Sbjct: 112 FESGNMTQVQVSLLALAGKAKTKGLQSDVDIGVKYSEKQQRNFDDATMKAGQCVIGLQMG 171
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 788
TNK ASQSGM ++G RH+ D + L I LQMGTNKCASQ GM+ G RHI
Sbjct: 172 TNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIY 231
Query: 789 DIKCESN-CPEAASIINLQY 845
D K ++ C ++ + + Y
Sbjct: 232 DTKLGTDKCDNSSMSLQMGY 251
>sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle)
Length = 292
Score = 131 bits (329), Expect = 3e-30
Identities = 94/264 (35%), Positives = 137/264 (51%), Gaps = 7/264 (2%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
NR +G+ + +L KYDP +E++ WI TG I +N LK+G IL +L
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDPQTERQLRVWIEGATGRRIG---DNFMDGLKDGVILCEL 63
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
IN + P S ++ P + + ++ENI FLRA + +GV +F+ DL
Sbjct: 64 INKLQ------PGSVQKVNDPVQN------WHKLENIGNFLRAIKHYGVKPHDIFEANDL 111
Query: 438 FESRNMAQVLNSILQLGTECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGT 617
FE+ N QV ++++ L ++ + G + L G + R+F E+LR G II LQ GT
Sbjct: 112 FENTNHTQVQSTLIALASQAKTKGNNVGL-GVKYAEKQQRRFQPEKLREGRNIIGLQMGT 170
Query: 618 NKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIAD 791
NKFASQ GM ++G RH+ D + + I LQMGTNK ASQ GM+ G R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230
Query: 792 -----IKCESNCPEAASIINLQYG 848
+C++N II LQ G
Sbjct: 231 PSLGMERCDTN------IIGLQMG 248
>sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (Calponin, non-muscle
isoform)
Length = 330
Score = 130 bits (327), Expect = 5e-30
Identities = 90/260 (34%), Positives = 137/260 (52%), Gaps = 3/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + ++ SKYD +E++ WI ++TG I N Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGMGIGT---NFQLGLKDGIILCEL 61
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
IN + P S ++ N + + Q+ENI F++A +++G+ +F+ DL
Sbjct: 62 INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 438 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FE+ NM QV +++ L + GF + G + R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGMSI-GAVRHIA 788
TNK ASQ+GM ++G RH+ D + + I LQMGTNK ASQ GMS G R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMSAPGTRRDIY 229
Query: 789 DIKCESNCPEAASIINLQYG 848
D K P S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248
>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
Length = 200
Score = 129 bits (324), Expect = 1e-29
Identities = 81/206 (39%), Positives = 117/206 (56%), Gaps = 7/206 (3%)
Frame = +3
Query: 72 MANRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNI---PLGRENVQHHLKNGQ 242
MAN+ +G+ + ++ KYD + E +EWI GS++ GR Q LKNG
Sbjct: 1 MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGI 60
Query: 243 ILVKLINVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLF 422
+L +L+N +Y PD ++ K P TM FKQME I FL+AAE +GV + +F
Sbjct: 61 VLSQLVNSLY------PDGSKPVKIPDSPPTMV--FKQMEQIAQFLKAAEDYGVVKTDMF 112
Query: 423 QTVDLFESRNMAQVLNSILQLG----TECQRNGFSGPLCGPRPTSSNFRQFSDEQLRMGE 590
QTVDLFE+++MA V +++ LG T+ + P + + R+FS+ QL+ G+
Sbjct: 113 QTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGK 172
Query: 591 GIINLQSGTNKFASQSGMSFGGVRHI 668
II LQ GTNK ASQ+GMS+G R I
Sbjct: 173 NIIGLQMGTNKGASQAGMSYGRPRQI 198
>sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform)
Length = 329
Score = 128 bits (322), Expect = 2e-29
Identities = 89/260 (34%), Positives = 138/260 (53%), Gaps = 3/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + ++ SKYD +E++ WI ++TG +I N Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIG---PNFQLGLKDGIILCEL 61
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
IN + P S ++ N + + Q+ENI F++A +++G+ +F+ DL
Sbjct: 62 INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 438 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FE+ NM QV +++ L + GF + G + R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGM-SIGAVRHIA 788
TNK ASQ+GM ++G RH+ D + + I LQMGTNK ASQ GM + G R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229
Query: 789 DIKCESNCPEAASIINLQYG 848
D K P S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248
>sp|P14318|MP20_DROME Muscle-specific protein 20
Length = 184
Score = 128 bits (322), Expect = 2e-29
Identities = 72/191 (37%), Positives = 113/191 (59%), Gaps = 1/191 (0%)
Frame = +3
Query: 99 IDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKLINVIYER 278
++ A+ ++ SK +P+ ++EA EWI + P G ++ + LK+GQ+L KLINV+
Sbjct: 3 LERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL--- 58
Query: 279 TPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDLFESRNMA 458
+PN + N+ FK MENI F +A + +GVP +FQTVDL+E +++A
Sbjct: 59 SPNAVP---------KVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIA 109
Query: 459 QVLNSILQLGTECQRNG-FSGPLCGPRPTSSNFRQFSDEQLRMGEGIINLQSGTNKFASQ 635
V N+I LG ++ F GP GP+P R F++EQL+ G+ I+ LQ+G+NK A+Q
Sbjct: 110 NVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGATQ 169
Query: 636 SGMSFGGVRHI 668
+G + G R I
Sbjct: 170 AGQNLGAGRKI 180
>sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform)
Length = 330
Score = 128 bits (321), Expect = 2e-29
Identities = 89/260 (34%), Positives = 137/260 (52%), Gaps = 3/260 (1%)
Frame = +3
Query: 78 NRTQDFGIDVAISRRLNSKYDPDSEQEAIEWINQLTGSNIPLGRENVQHHLKNGQILVKL 257
N+ +G+ + ++ SKYD +E++ WI ++TG I N Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGLGIGT---NFQLGLKDGIILCEL 61
Query: 258 INVIYERTPNLPDSARREKHPFRYNTMTAPFKQMENIQIFLRAAESFGVPRQSLFQTVDL 437
IN + P S ++ N + + Q+ENI F++A +++G+ +F+ DL
Sbjct: 62 INKLQ------PGSVKK------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 438 FESRNMAQVLNSILQLGTECQRNGFSGPL-CGPRPTSSNFRQFSDEQLRMGEGIINLQSG 614
FE+ NM QV +++ L + GF + G + R+F + +L+ G+ +I LQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 615 TNKFASQSGM-SFGGVRHIADIRCDDLNDSGKGIIGLQMGTNKCASQKGM-SIGAVRHIA 788
TNK ASQ+GM ++G RH+ D + + I LQMGTNK ASQ GM + G R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229
Query: 789 DIKCESNCPEAASIINLQYG 848
D K P S I+LQ G
Sbjct: 230 DQKLTLQ-PVDNSTISLQMG 248
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,453,854
Number of Sequences: 369166
Number of extensions: 2286203
Number of successful extensions: 5403
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5066
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5282
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)