Planaria EST Database

DrC_00308

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00308
         (755 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O15145|ARPC3_HUMAN  Actin-related protein 2/3 complex sub...   216   7e-56
sp|Q9JM76|ARPC3_MOUSE  Actin-related protein 2/3 complex sub...   215   9e-56
sp|Q9XWV3|ARPC3_CAEEL  Probable actin-related protein 2/3 co...   171   1e-42
sp|Q9Y7J4|ARPC3_SCHPO  ARP2/3 complex 21 kDa subunit (p21-ARC)    167   2e-41
sp|Q05933|ARPC3_YEAST  ARP2/3 complex 21 kDa subunit (p21-ARC)    155   1e-37
sp|O96624|ARPC3_DICDI  Actin-related protein 2/3 complex sub...   144   3e-34
sp|O15604|ARPC3_ENTHI  Probable actin-related protein 2/3 co...    54   5e-07
sp|P32790|SLA1_YEAST  Cytoskeleton assembly control protein ...    30   7.0  

>sp|O15145|ARPC3_HUMAN Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
           subunit) (p21-ARC)
          Length = 178

 Score =  216 bits (549), Expect = 7e-56
 Identities = 99/177 (55%), Positives = 133/177 (75%), Gaps = 1/177 (0%)
 Frame = +2

Query: 32  AYHSSFNEYCSS-IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEIKS 208
           AYHSS  +  +  IGNMALLP+R++F+GPAP    D D++DE IY+FKAN+FFK YEIK+
Sbjct: 3   AYHSSLMDPDTKLIGNMALLPIRSQFKGPApreTKDTDIVDEAIYYFKANVFFKNYEIKN 62

Query: 209 PADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARP 388
            ADR +IYITL+I ECL++LQ+C +K+QG KE+  L ++ F +PG+PGFP  LNA+YA+P
Sbjct: 63  EADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFP--LNAIYAKP 120

Query: 389 KDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAPG 559
            +  E E+M+ Y  Q+RQET LRL EKVF    D P+KWW CF +R+FM ++ S PG
Sbjct: 121 ANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPG 177

>sp|Q9JM76|ARPC3_MOUSE Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
           subunit) (p21-ARC)
          Length = 178

 Score =  215 bits (548), Expect = 9e-56
 Identities = 100/177 (56%), Positives = 132/177 (74%), Gaps = 1/177 (0%)
 Frame = +2

Query: 32  AYHSSFNEYCSS-IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEIKS 208
           AYHSS  +  +  IGNMALLPLR++F+GPAP    D D++DE IY+FKAN+FFK YEIK+
Sbjct: 3   AYHSSLMDPDTKLIGNMALLPLRSQFKGPApreTKDTDIVDEAIYYFKANVFFKNYEIKN 62

Query: 209 PADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARP 388
            ADR +IYITL+I ECL++LQ+C +K+QG KE+  L ++ F +PG+PGFP  LNA+YA+P
Sbjct: 63  EADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFP--LNAIYAKP 120

Query: 389 KDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAPG 559
               E E+M+ Y  Q+RQET LRL EKVF    D P+KWW CF +R+FM ++ S PG
Sbjct: 121 ASKQEDEMMRAYLQQLRQETGLRLCEKVFDPQSDKPSKWWTCFVKRQFMNKSLSGPG 177

>sp|Q9XWV3|ARPC3_CAEEL Probable actin-related protein 2/3 complex subunit 3 (ARP2/3
           complex 21 kDa subunit) (p21-ARC)
          Length = 183

 Score =  171 bits (434), Expect = 1e-42
 Identities = 86/181 (47%), Positives = 120/181 (66%), Gaps = 5/181 (2%)
 Frame = +2

Query: 32  AYHSSFNEYCSSIG----NMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYE 199
           AYHS F+     +     NM  LP+RT F+GPAP T+ D D+IDE + +FK NIFF+++E
Sbjct: 3   AYHSKFDTELKVLPLGNTNMGKLPIRTNFKGPAPQTNQD-DIIDEALTYFKPNIFFREFE 61

Query: 200 IKSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSR-FDLPGDPGFpreLNAL 376
           IK PADR +IY+  +I ECLR+LQ+  NK  G K+L ALA+S    +PG+ GFP  LN++
Sbjct: 62  IKGPADRTMIYLIFYITECLRKLQKSPNKIAGQKDLHALALSHLLPIPGENGFP--LNSM 119

Query: 377 YARPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAP 556
           Y  P+   +++ M+ Y  QIRQE   RL +  F + QD P+KWW+CFARR+FM++     
Sbjct: 120 YKAPQSKPDEDEMRAYLQQIRQEIGARLCDLAFPDPQDRPSKWWLCFARRRFMDKGLVGQ 179

Query: 557 G 559
           G
Sbjct: 180 G 180

>sp|Q9Y7J4|ARPC3_SCHPO ARP2/3 complex 21 kDa subunit (p21-ARC)
          Length = 174

 Score =  167 bits (424), Expect = 2e-41
 Identities = 88/174 (50%), Positives = 114/174 (65%), Gaps = 3/174 (1%)
 Frame = +2

Query: 32  AYHSSFNEYCS--SIGNMALLPLRTKFRGPA-PCTDIDKDVIDEGIYFFKANIFFKKYEI 202
           AYHSSF       + GN+A+LPL+TKFRGPA P  +   D+IDE I  F+AN FF+ +EI
Sbjct: 3   AYHSSFLSLTDVPTTGNIAMLPLKTKFRGPAYPADESQMDIIDECIGLFRANCFFRNFEI 62

Query: 203 KSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYA 382
           K PADR +IY TLFI ECL R+    N     ++L +LA+  F +PG  GFP  LNALYA
Sbjct: 63  KGPADRTLIYGTLFISECLGRVNGL-NYRDAERQLNSLALENFSIPGSAGFP--LNALYA 119

Query: 383 RPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQA 544
            P    + EIM+ Y  Q RQE A RLL  V+  ++D P+KWW CF++R+FM +A
Sbjct: 120 PPLSPQDAEIMRTYLTQFRQELAYRLLSHVYATEKDHPSKWWTCFSKRRFMNKA 173

>sp|Q05933|ARPC3_YEAST ARP2/3 complex 21 kDa subunit (p21-ARC)
          Length = 178

 Score =  155 bits (392), Expect = 1e-37
 Identities = 85/177 (48%), Positives = 119/177 (67%), Gaps = 6/177 (3%)
 Frame = +2

Query: 32  AYHSSFNEYCSS---IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEI 202
           AYHS+F    ++   +GN ALLPL TKFRGPA  ++ D D+IDE +  F+AN FFK +EI
Sbjct: 3   AYHSTFPVDPNTDRMVGNFALLPLNTKFRGPAYPSNSDYDIIDECLDLFRANSFFKNFEI 62

Query: 203 KSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYA 382
           KSPADRV+IY  LFI +CL  L+  T+  +  K L  +A+  F LPG PGFP  LN +Y 
Sbjct: 63  KSPADRVLIYGILFINDCLAHLKITTSFNEAVKVLTNVALDNFTLPGTPGFP--LNNVYQ 120

Query: 383 RP-KDLNEQEIMKGYFLQIRQETALRLLEKVF--LNDQDPPNKWWMCFARRKFMEQA 544
            P +D N  +++K Y  Q RQE A+RLLE+V+   + ++ P+K+W+ F RR+FM ++
Sbjct: 121 VPVQDHNSMDLLKTYIQQFRQELAMRLLERVYSSTDSKEYPSKFWLAFTRRRFMNKS 177

>sp|O96624|ARPC3_DICDI Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
           subunit) (p21-ARC)
          Length = 174

 Score =  144 bits (362), Expect = 3e-34
 Identities = 70/176 (39%), Positives = 108/176 (61%), Gaps = 4/176 (2%)
 Frame = +2

Query: 29  MAYHSSFNEYCSS---IGNMALLPLRTKFRGPAPCTDIDK-DVIDEGIYFFKANIFFKKY 196
           M YHS FN+  +    +GN+ +LPL+T  +GPAP  D +  D+IDE +  FKANI F+ +
Sbjct: 1   MVYHSQFNDESAGFRLVGNVPILPLKTTHKGPAPKGDANSVDIIDEALDLFKANILFRNF 60

Query: 197 EIKSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNAL 376
           E++   DRV+IY+TL+I +CL ++    NK    K L  +A  +F +PG+  FP  L  L
Sbjct: 61  EVQGNGDRVLIYLTLYITKCLLKIAPM-NKADAEKALFLIAQEQFSIPGESAFP--LGGL 117

Query: 377 YARPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQA 544
              P   +  + ++ YF Q+R E  +RL ++V+  D    NKWW+CF++RKF+ +A
Sbjct: 118 VTVPNTRDAADTLRQYFTQLRLELGVRLCQRVYAVDPSKANKWWICFSKRKFLNKA 173

>sp|O15604|ARPC3_ENTHI Probable actin-related protein 2/3 complex subunit 3 (ARP2/3
           complex 21 kDa subunit) (p21-ARC)
          Length = 128

 Score = 53.9 bits (128), Expect = 5e-07
 Identities = 35/112 (31%), Positives = 56/112 (50%), Gaps = 4/112 (3%)
 Frame = +2

Query: 32  AYHSSFN-EYCSSIGNMALLPLRTKFRGPAP--CTDIDKDVIDEGIYFFKANIFFKKYEI 202
           AYHS  + +     GN  L+  +TK +          D D++DE I ++KAN+F+K  ++
Sbjct: 8   AYHSELDKDIVQKAGNFPLMQFKTKVKVNPKFLLKGDDMDIVDEAITYYKANVFYKNLDM 67

Query: 203 K-SPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGF 355
               AD+++ Y+ +F L+ L        K    K   +LA+  F LPGD  F
Sbjct: 68  SICEADKLLAYL-MFYLQSLLLTFTGRTKVDCDKMAFSLAIENFGLPGDGKF 118

>sp|P32790|SLA1_YEAST Cytoskeleton assembly control protein SLA1
          Length = 1244

 Score = 30.0 bits (66), Expect = 7.0
 Identities = 19/95 (20%), Positives = 41/95 (43%)
 Frame = +2

Query: 230 YITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARPKDLNEQE 409
           ++ L  ++  R L+  T  T   +E++ +         DPG      A  ++ + + + +
Sbjct: 304 HMFLEFVDPYRSLELHTGNTTTCEEIMNIIGEYKGASRDPGLREVEMASKSKKRGIVQYD 363

Query: 410 IMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMC 514
            M     + + E  ++  +KV++ D      WWMC
Sbjct: 364 FMA----ESQDELTIKSGDKVYILDDKKSKDWWMC 394

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,584,136
Number of Sequences: 369166
Number of extensions: 1656349
Number of successful extensions: 4237
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4068
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4224
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6921714800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00308

  1. Dr_sW_008_E02
  2. Dr_sW_006_H15
  3. Dr_sW_022_O16