Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_E02
(680 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O15145|ARPC3_HUMAN Actin-related protein 2/3 complex sub... 216 6e-56
sp|Q9JM76|ARPC3_MOUSE Actin-related protein 2/3 complex sub... 215 8e-56
sp|Q9XWV3|ARPC3_CAEEL Probable actin-related protein 2/3 co... 171 1e-42
sp|Q9Y7J4|ARPC3_SCHPO ARP2/3 complex 21 kDa subunit (p21-ARC) 167 2e-41
sp|Q05933|ARPC3_YEAST ARP2/3 complex 21 kDa subunit (p21-ARC) 155 9e-38
sp|O96624|ARPC3_DICDI Actin-related protein 2/3 complex sub... 144 3e-34
sp|O15604|ARPC3_ENTHI Probable actin-related protein 2/3 co... 54 4e-07
sp|P32790|SLA1_YEAST Cytoskeleton assembly control protein ... 30 5.9
>sp|O15145|ARPC3_HUMAN Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
subunit) (p21-ARC)
Length = 178
Score = 216 bits (549), Expect = 6e-56
Identities = 99/177 (55%), Positives = 133/177 (75%), Gaps = 1/177 (0%)
Frame = +2
Query: 32 AYHSSFNEYCSS-IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEIKS 208
AYHSS + + IGNMALLP+R++F+GPAP D D++DE IY+FKAN+FFK YEIK+
Sbjct: 3 AYHSSLMDPDTKLIGNMALLPIRSQFKGPApreTKDTDIVDEAIYYFKANVFFKNYEIKN 62
Query: 209 PADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARP 388
ADR +IYITL+I ECL++LQ+C +K+QG KE+ L ++ F +PG+PGFP LNA+YA+P
Sbjct: 63 EADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFP--LNAIYAKP 120
Query: 389 KDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAPG 559
+ E E+M+ Y Q+RQET LRL EKVF D P+KWW CF +R+FM ++ S PG
Sbjct: 121 ANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPG 177
>sp|Q9JM76|ARPC3_MOUSE Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
subunit) (p21-ARC)
Length = 178
Score = 215 bits (548), Expect = 8e-56
Identities = 100/177 (56%), Positives = 132/177 (74%), Gaps = 1/177 (0%)
Frame = +2
Query: 32 AYHSSFNEYCSS-IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEIKS 208
AYHSS + + IGNMALLPLR++F+GPAP D D++DE IY+FKAN+FFK YEIK+
Sbjct: 3 AYHSSLMDPDTKLIGNMALLPLRSQFKGPApreTKDTDIVDEAIYYFKANVFFKNYEIKN 62
Query: 209 PADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARP 388
ADR +IYITL+I ECL++LQ+C +K+QG KE+ L ++ F +PG+PGFP LNA+YA+P
Sbjct: 63 EADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFP--LNAIYAKP 120
Query: 389 KDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAPG 559
E E+M+ Y Q+RQET LRL EKVF D P+KWW CF +R+FM ++ S PG
Sbjct: 121 ASKQEDEMMRAYLQQLRQETGLRLCEKVFDPQSDKPSKWWTCFVKRQFMNKSLSGPG 177
>sp|Q9XWV3|ARPC3_CAEEL Probable actin-related protein 2/3 complex subunit 3 (ARP2/3
complex 21 kDa subunit) (p21-ARC)
Length = 183
Score = 171 bits (434), Expect = 1e-42
Identities = 86/181 (47%), Positives = 120/181 (66%), Gaps = 5/181 (2%)
Frame = +2
Query: 32 AYHSSFNEYCSSIG----NMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYE 199
AYHS F+ + NM LP+RT F+GPAP T+ D D+IDE + +FK NIFF+++E
Sbjct: 3 AYHSKFDTELKVLPLGNTNMGKLPIRTNFKGPAPQTNQD-DIIDEALTYFKPNIFFREFE 61
Query: 200 IKSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSR-FDLPGDPGFpreLNAL 376
IK PADR +IY+ +I ECLR+LQ+ NK G K+L ALA+S +PG+ GFP LN++
Sbjct: 62 IKGPADRTMIYLIFYITECLRKLQKSPNKIAGQKDLHALALSHLLPIPGENGFP--LNSM 119
Query: 377 YARPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQAFSAP 556
Y P+ +++ M+ Y QIRQE RL + F + QD P+KWW+CFARR+FM++
Sbjct: 120 YKAPQSKPDEDEMRAYLQQIRQEIGARLCDLAFPDPQDRPSKWWLCFARRRFMDKGLVGQ 179
Query: 557 G 559
G
Sbjct: 180 G 180
>sp|Q9Y7J4|ARPC3_SCHPO ARP2/3 complex 21 kDa subunit (p21-ARC)
Length = 174
Score = 167 bits (424), Expect = 2e-41
Identities = 88/174 (50%), Positives = 114/174 (65%), Gaps = 3/174 (1%)
Frame = +2
Query: 32 AYHSSFNEYCS--SIGNMALLPLRTKFRGPA-PCTDIDKDVIDEGIYFFKANIFFKKYEI 202
AYHSSF + GN+A+LPL+TKFRGPA P + D+IDE I F+AN FF+ +EI
Sbjct: 3 AYHSSFLSLTDVPTTGNIAMLPLKTKFRGPAYPADESQMDIIDECIGLFRANCFFRNFEI 62
Query: 203 KSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYA 382
K PADR +IY TLFI ECL R+ N ++L +LA+ F +PG GFP LNALYA
Sbjct: 63 KGPADRTLIYGTLFISECLGRVNGL-NYRDAERQLNSLALENFSIPGSAGFP--LNALYA 119
Query: 383 RPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQA 544
P + EIM+ Y Q RQE A RLL V+ ++D P+KWW CF++R+FM +A
Sbjct: 120 PPLSPQDAEIMRTYLTQFRQELAYRLLSHVYATEKDHPSKWWTCFSKRRFMNKA 173
>sp|Q05933|ARPC3_YEAST ARP2/3 complex 21 kDa subunit (p21-ARC)
Length = 178
Score = 155 bits (392), Expect = 9e-38
Identities = 85/177 (48%), Positives = 119/177 (67%), Gaps = 6/177 (3%)
Frame = +2
Query: 32 AYHSSFNEYCSS---IGNMALLPLRTKFRGPAPCTDIDKDVIDEGIYFFKANIFFKKYEI 202
AYHS+F ++ +GN ALLPL TKFRGPA ++ D D+IDE + F+AN FFK +EI
Sbjct: 3 AYHSTFPVDPNTDRMVGNFALLPLNTKFRGPAYPSNSDYDIIDECLDLFRANSFFKNFEI 62
Query: 203 KSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYA 382
KSPADRV+IY LFI +CL L+ T+ + K L +A+ F LPG PGFP LN +Y
Sbjct: 63 KSPADRVLIYGILFINDCLAHLKITTSFNEAVKVLTNVALDNFTLPGTPGFP--LNNVYQ 120
Query: 383 RP-KDLNEQEIMKGYFLQIRQETALRLLEKVF--LNDQDPPNKWWMCFARRKFMEQA 544
P +D N +++K Y Q RQE A+RLLE+V+ + ++ P+K+W+ F RR+FM ++
Sbjct: 121 VPVQDHNSMDLLKTYIQQFRQELAMRLLERVYSSTDSKEYPSKFWLAFTRRRFMNKS 177
>sp|O96624|ARPC3_DICDI Actin-related protein 2/3 complex subunit 3 (ARP2/3 complex 21 kDa
subunit) (p21-ARC)
Length = 174
Score = 144 bits (362), Expect = 3e-34
Identities = 70/176 (39%), Positives = 108/176 (61%), Gaps = 4/176 (2%)
Frame = +2
Query: 29 MAYHSSFNEYCSS---IGNMALLPLRTKFRGPAPCTDIDK-DVIDEGIYFFKANIFFKKY 196
M YHS FN+ + +GN+ +LPL+T +GPAP D + D+IDE + FKANI F+ +
Sbjct: 1 MVYHSQFNDESAGFRLVGNVPILPLKTTHKGPAPKGDANSVDIIDEALDLFKANILFRNF 60
Query: 197 EIKSPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNAL 376
E++ DRV+IY+TL+I +CL ++ NK K L +A +F +PG+ FP L L
Sbjct: 61 EVQGNGDRVLIYLTLYITKCLLKIAPM-NKADAEKALFLIAQEQFSIPGESAFP--LGGL 117
Query: 377 YARPKDLNEQEIMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMCFARRKFMEQA 544
P + + ++ YF Q+R E +RL ++V+ D NKWW+CF++RKF+ +A
Sbjct: 118 VTVPNTRDAADTLRQYFTQLRLELGVRLCQRVYAVDPSKANKWWICFSKRKFLNKA 173
>sp|O15604|ARPC3_ENTHI Probable actin-related protein 2/3 complex subunit 3 (ARP2/3
complex 21 kDa subunit) (p21-ARC)
Length = 128
Score = 53.9 bits (128), Expect = 4e-07
Identities = 35/112 (31%), Positives = 56/112 (50%), Gaps = 4/112 (3%)
Frame = +2
Query: 32 AYHSSFN-EYCSSIGNMALLPLRTKFRGPAP--CTDIDKDVIDEGIYFFKANIFFKKYEI 202
AYHS + + GN L+ +TK + D D++DE I ++KAN+F+K ++
Sbjct: 8 AYHSELDKDIVQKAGNFPLMQFKTKVKVNPKFLLKGDDMDIVDEAITYYKANVFYKNLDM 67
Query: 203 K-SPADRVIIYITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGF 355
AD+++ Y+ +F L+ L K K +LA+ F LPGD F
Sbjct: 68 SICEADKLLAYL-MFYLQSLLLTFTGRTKVDCDKMAFSLAIENFGLPGDGKF 118
>sp|P32790|SLA1_YEAST Cytoskeleton assembly control protein SLA1
Length = 1244
Score = 30.0 bits (66), Expect = 5.9
Identities = 19/95 (20%), Positives = 41/95 (43%)
Frame = +2
Query: 230 YITLFILECLRRLQRCTNKTQGSKELLALAMSRFDLPGDPGFpreLNALYARPKDLNEQE 409
++ L ++ R L+ T T +E++ + DPG A ++ + + + +
Sbjct: 304 HMFLEFVDPYRSLELHTGNTTTCEEIMNIIGEYKGASRDPGLREVEMASKSKKRGIVQYD 363
Query: 410 IMKGYFLQIRQETALRLLEKVFLNDQDPPNKWWMC 514
M + + E ++ +KV++ D WWMC
Sbjct: 364 FMA----ESQDELTIKSGDKVYILDDKKSKDWWMC 394
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,728,316
Number of Sequences: 369166
Number of extensions: 1522839
Number of successful extensions: 3932
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3919
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5782011865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)