Planaria EST Database

DrC_00286

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00286
         (881 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6ZWX6|IF2A_MOUSE  Eukaryotic translation initiation fact...   269   9e-72
sp|P05198|IF2A_HUMAN  Eukaryotic translation initiation fact...   266   4e-71
sp|P41374|IF2A_DROME  Eukaryotic translation initiation fact...   249   9e-66
sp|P56286|IF2A_SCHPO  Eukaryotic translation initiation fact...   193   5e-49
sp|P20459|IF2A_YEAST  Eukaryotic translation initiation fact...   192   1e-48
sp|Q8TY86|IF2A_METKA  Translation initiation factor 2 alpha ...    69   2e-11
sp|Q8U1R5|IF2A_PYRFU  Translation initiation factor 2 alpha ...    65   2e-10
sp|Q57581|IF2A_METJA  Translation initiation factor 2 alpha ...    63   1e-09
sp|Q973G0|IF2A_SULTO  Translation initiation factor 2 alpha ...    63   1e-09
sp|Q5JE49|IF2A_PYRKO  Translation initiation factor 2 alpha ...    62   3e-09

>sp|Q6ZWX6|IF2A_MOUSE Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
 sp|P68101|IF2A_RAT Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
 sp|P68102|IF2A_BOVIN Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
          Length = 315

 Score =  269 bits (687), Expect = 9e-72
 Identities = 135/243 (55%), Positives = 179/243 (73%), Gaps = 1/243 (0%)
 Frame = +3

Query: 3   RIRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRR 182
           RIRS+NKL+R+GRNE V VIRVD +K YIDLSKR+VS EE  +CE+KF  SKTV  ILR 
Sbjct: 55  RIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRH 114

Query: 183 LAEELKFETDEQLEDLFNKTAWFFDRKYSRKG-ASFDVFKQAVTNPEVLDECGLENSVKE 359
           +AE L++  DEQLE LF +TAW FD KY R G  ++D FK AV++P +LD   L    +E
Sbjct: 115 VAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDERE 174

Query: 360 KLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQSPQ 539
            L+ +INRR+ PQAVKIR+D EV+C+ +EGIDA+K ALR GL+ S++ + IKINLI  P+
Sbjct: 175 VLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPR 234

Query: 540 FMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRLAELE 719
           ++MT  TL++ +GLS LN+A+ VI+E IE K+G F +  +PKVV+D DE  L ++L  LE
Sbjct: 235 YVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLE 294

Query: 720 EAN 728
             N
Sbjct: 295 REN 297

>sp|P05198|IF2A_HUMAN Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF-2alpha) (EIF-2A)
          Length = 315

 Score =  266 bits (681), Expect = 4e-71
 Identities = 133/243 (54%), Positives = 179/243 (73%), Gaps = 1/243 (0%)
 Frame = +3

Query: 3   RIRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRR 182
           RIRS+NKL+R+GRNE V VIRVD +K YIDLSKR+VS EE  +CE+KF  SKTV  ILR 
Sbjct: 55  RIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRH 114

Query: 183 LAEELKFETDEQLEDLFNKTAWFFDRKYSRKG-ASFDVFKQAVTNPEVLDECGLENSVKE 359
           +AE L++  DEQLE LF +TAW FD KY R G  ++D FK AV++P +LD   L    +E
Sbjct: 115 VAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDERE 174

Query: 360 KLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQSPQ 539
            L+ +INRR+ PQAVKIR+D EV+C+ +EGIDA+K ALR GL+ S++ + IKINLI  P+
Sbjct: 175 VLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPR 234

Query: 540 FMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRLAELE 719
           ++MT  TL++ +GLS L++A+ VI+E IE K+G F +  +PKVV+D DE  L +++  LE
Sbjct: 235 YVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLE 294

Query: 720 EAN 728
             N
Sbjct: 295 REN 297

>sp|P41374|IF2A_DROME Eukaryotic translation initiation factor 2 alpha subunit
           (eIF-2-alpha)
          Length = 341

 Score =  249 bits (635), Expect = 9e-66
 Identities = 123/242 (50%), Positives = 171/242 (70%)
 Frame = +3

Query: 3   RIRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRR 182
           RIRS+NKL+RVG+ E V VIRVD +K YIDLSKR+VS E++ +C E+FA +K +N +LR 
Sbjct: 54  RIRSINKLIRVGKTEPVVVIRVDKEKGYIDLSKRRVSPEDVEKCTERFAKAKAINSLLRH 113

Query: 183 LAEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLENSVKEK 362
           +A+ L FE +E+LEDL+ KTAW F++KY+ K  ++D+FKQ+VT+P V DEC LE   KE 
Sbjct: 114 VADILGFEGNEKLEDLYQKTAWHFEKKYNNKTVAYDIFKQSVTDPTVFDECNLEPETKEV 173

Query: 363 LLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQSPQF 542
           LL++I R+++   VKIR+D E SC+ +EGIDA+K +L KGL++S++EL I+INLI  P +
Sbjct: 174 LLSNIKRKLVSPTVKIRADIECSCYGYEGIDAVKASLTKGLELSTEELPIRINLIAPPLY 233

Query: 543 MMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRLAELEE 722
           +MT  T  K  GL  L  AI  IR       G FK++  PK+V+  DE +L +RL   E 
Sbjct: 234 VMTTSTTKKTDGLKALEVAIEHIRAKTSEYDGEFKVIMAPKLVTAIDEADLARRLERAEA 293

Query: 723 AN 728
            N
Sbjct: 294 EN 295

>sp|P56286|IF2A_SCHPO Eukaryotic translation initiation factor 2 alpha subunit
           (eIF-2-alpha)
          Length = 306

 Score =  193 bits (491), Expect = 5e-49
 Identities = 109/245 (44%), Positives = 158/245 (64%), Gaps = 3/245 (1%)
 Frame = +3

Query: 3   RIRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRR 182
           RIRSV K +RVGRNEVV V+RVD +K YIDLSKR+VS E++ +CEE+F  SK V+ I+R 
Sbjct: 55  RIRSVQKHIRVGRNEVVVVLRVDKEKGYIDLSKRRVSPEDVVKCEERFNKSKAVHSIMRH 114

Query: 183 LAEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLE---NSV 353
           +AE+     +  LE ++    W   RKY   G ++D FK A++NP+ + E GLE   + V
Sbjct: 115 IAEK----HNVPLETMYTTIGWPLYRKY---GHAYDAFKLAISNPDHVFE-GLEPPKSGV 166

Query: 354 KEKLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQS 533
              LLA I+RR+ PQ +KIR+D EV+CF +EGI+AIK AL+   D+ ++E+ IK+ L+  
Sbjct: 167 INDLLAQISRRLTPQPIKIRADVEVTCFGYEGINAIKAALKAAEDVHTEEVPIKVKLVAP 226

Query: 534 PQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRLAE 713
           P +++    LDK  GL  L EAI  I ++I +  G+  +  +PK VS+ DE  L   + +
Sbjct: 227 PLYVLLTNALDKSLGLKKLEEAIGAIEKSITASNGTCTVKMKPKAVSETDELELADLMKK 286

Query: 714 LEEAN 728
            E+ N
Sbjct: 287 FEKEN 291

>sp|P20459|IF2A_YEAST Eukaryotic translation initiation factor 2 alpha subunit
           (eIF-2-alpha)
          Length = 304

 Score =  192 bits (488), Expect = 1e-48
 Identities = 107/246 (43%), Positives = 162/246 (65%), Gaps = 3/246 (1%)
 Frame = +3

Query: 3   RIRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRR 182
           RIRS+ KL+RVG+N+V  V+RVD +K YIDLSKR+VS+E+I +CEEK+  SKTV+ ILR 
Sbjct: 55  RIRSIQKLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKYQKSKTVHSILRY 114

Query: 183 LAEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLE---NSV 353
            AE+ +      LE+L+   AW   RK+   G +++ FK ++ +  V +  G+E     V
Sbjct: 115 CAEKFQIP----LEELYKTIAWPLSRKF---GHAYEAFKLSIIDETVWE--GIEPPSKDV 165

Query: 354 KEKLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQS 533
            ++L   I++R+ PQAVKIR+D EVSCF++EGIDAIK AL+   DMS++++++K+ L+ +
Sbjct: 166 LDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAA 225

Query: 534 PQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRLAE 713
           P +++T Q LDK+KG+  L  AI  I E I    G   +   PK V+  ++  L + L E
Sbjct: 226 PLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAEL-QALLE 284

Query: 714 LEEANN 731
            +E +N
Sbjct: 285 SKELDN 290

>sp|Q8TY86|IF2A_METKA Translation initiation factor 2 alpha subunit (eIF-2-alpha)
           (aIF2-alpha)
          Length = 267

 Score = 68.6 bits (166), Expect = 2e-11
 Identities = 47/213 (22%), Positives = 99/213 (46%), Gaps = 2/213 (0%)
 Frame = +3

Query: 6   IRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRRL 185
           ++++   ++ G+  V  VIRV+  +KY +LS R+V+  +     +++   +   ++L   
Sbjct: 51  VKNIRDYVKEGQKVVAKVIRVNPKRKYANLSLRKVTDHQRKEKLKEWKREQRAEKLLEMA 110

Query: 186 AEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTN--PEVLDECGLENSVKE 359
           AEEL  + DE  E+   K            G+ +D  ++A     PE L + G+     E
Sbjct: 111 AEELGKDLDEAYEEAGYKLI-------EEYGSLYDALERAAAEEGPEPLLKAGVPEEWAE 163

Query: 360 KLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSKELEIKINLIQSPQ 539
           KL       + P  VKI +  +++C    G++ I+ AL K  +    ++++++  + +P+
Sbjct: 164 KLAELAIENIEPGRVKIEAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPR 223

Query: 540 FMMTCQTLDKEKGLSTLNEAIRVIREAIESKKG 638
           + +T    D       + +A +   + +E   G
Sbjct: 224 YRITVDAPDYRTAEKMVRKAAQAAIDHVEEHGG 256

>sp|Q8U1R5|IF2A_PYRFU Translation initiation factor 2 alpha subunit (eIF-2-alpha)
           (aIF2-alpha)
          Length = 275

 Score = 65.1 bits (157), Expect = 2e-10
 Identities = 57/237 (24%), Positives = 109/237 (45%), Gaps = 4/237 (1%)
 Frame = +3

Query: 6   IRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRRL 185
           ++++   LR G+  V  VIRVD  K +IDLS R+V+ ++     ++F  ++    +L+  
Sbjct: 51  VKNIRDYLREGQKVVAKVIRVDPKKGHIDLSLRRVTQQQRKAKLQEFKRAQKAENLLKLA 110

Query: 186 AEELKFETDEQLEDLF--NKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLENSVKE 359
           AE+L  + +E   +++   +  W         G  +  F+ A  N   + +  + +    
Sbjct: 111 AEKLGKDFEEAWREVWVPLENEW---------GEVYAAFEDAARNGIEVLKGYVPDEWLP 161

Query: 360 KLLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSS--KELEIKINLIQS 533
            L   I+  +    V I ++FE++     GI+ IK AL K  D ++  K++E+K   + +
Sbjct: 162 VLKEIIDSYVEVPTVTIDAEFEITVPKPNGIEIIKEALIKARDRANQEKDIEVKFTYLGA 221

Query: 534 PQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKR 704
           P++ +     D  K    L +    I   I+   G   LL + K +     + +KKR
Sbjct: 222 PRYRIDITAPDYYKAEEVLEDIAEEILRVIKEAGGEATLLRKEKRI-----RKVKKR 273

>sp|Q57581|IF2A_METJA Translation initiation factor 2 alpha subunit (eIF-2-alpha)
           (aIF2-alpha)
          Length = 266

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 43/192 (22%), Positives = 94/192 (48%), Gaps = 1/192 (0%)
 Frame = +3

Query: 6   IRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRRL 185
           ++++   ++VG+  V  V+RVD  K +IDLS ++V+ ++     +++   +  +++L R 
Sbjct: 51  VKNIRDHVKVGQRVVAKVLRVDERKGHIDLSLKRVTEQQKRAKVQEWKRFQRASKMLERA 110

Query: 186 AEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLENSVKEKL 365
           AE+L     + LE+ + +  +  + ++     +F+     +   EVLD+  +    K  L
Sbjct: 111 AEKL----GKSLEEAWEEVGYLLEDEFGELYNAFET--MVIEGKEVLDDLEISEEWKNVL 164

Query: 366 LADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSS-KELEIKINLIQSPQF 542
                  +    V++    E+  +  +GI  IK AL   L  +  +++E+KI  I +P++
Sbjct: 165 YEVAKESIELTNVEVEGVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKY 224

Query: 543 MMTCQTLDKEKG 578
            +     D + G
Sbjct: 225 RVVVIAPDYKSG 236

>sp|Q973G0|IF2A_SULTO Translation initiation factor 2 alpha subunit (eIF-2-alpha)
           (aIF2-alpha)
          Length = 263

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 50/220 (22%), Positives = 109/220 (49%), Gaps = 2/220 (0%)
 Frame = +3

Query: 6   IRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRRL 185
           ++++  +++ GR  VV VIRVD  K  ID+S ++V+ +E  +   ++   + V++IL  +
Sbjct: 51  VKNIRDVIKEGRKIVVKVIRVDKRKGTIDVSLKKVTEDEKRKKMSQWKKIQKVDKILEIV 110

Query: 186 AEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPE-VLDECGLENSVKEK 362
           ++++     E  E +    AW  + KY   G ++D   +AV   + +L   G+     + 
Sbjct: 111 SQKINKTEKEGWEQV----AWKLEDKY---GDAYDALVKAVKEGDSILKNAGVPEVWIKP 163

Query: 363 LLADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSSK-ELEIKINLIQSPQ 539
           L+ +I + +  + VK      +      GI+ I+  L   ++++   +++IKI  I +P+
Sbjct: 164 LMEEIGKHIEEKRVKKSEIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPR 223

Query: 540 FMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQ 659
           + +     D +     + E +  IRE  + +K  F ++ +
Sbjct: 224 YRIDIIGTDPKLLSKVMTEILDNIREISKEEKVEFNVVRK 263

>sp|Q5JE49|IF2A_PYRKO Translation initiation factor 2 alpha subunit (eIF-2-alpha)
           (aIF2-alpha)
          Length = 275

 Score = 61.6 bits (148), Expect = 3e-09
 Identities = 54/235 (22%), Positives = 109/235 (46%), Gaps = 2/235 (0%)
 Frame = +3

Query: 6   IRSVNKLLRVGRNEVVSVIRVDADKKYIDLSKRQVSAEEITRCEEKFANSKTVNQILRRL 185
           ++++   L+ G+  V  VIRVD +K +IDLS ++V+ ++     +++  ++    +L+  
Sbjct: 51  VKNIRDYLKEGQKIVAKVIRVDPEKGHIDLSLKRVNQQQRKAKLQEYKRAQKAENLLKMA 110

Query: 186 AEELKFETDEQLEDLFNKTAWFFDRKYSRKGASFDVFKQAVTNPEVLDECGLENSVKEKL 365
           AE+L     +  E  + +     + +Y   G  +  F+ A  N   + +  + +   E L
Sbjct: 111 AEKL----GKDFETAWREVWVPLEEEY---GEVYAAFEDAAQNGMDVLKGLISDEWIEAL 163

Query: 366 LADINRRMMPQAVKIRSDFEVSCFTFEGIDAIKHALRKGLDMSS--KELEIKINLIQSPQ 539
              I   +    V I ++FE++     GI+ IK AL K  D ++  K++E+K +   +P+
Sbjct: 164 KPIIEAYVEIPTVTIDAEFEITVPKPNGIEIIKEALIKARDRANQEKDIEVKFSYQGAPR 223

Query: 540 FMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKR 704
           + +     D  K    L +    I   I+   G   L+ + K +     + +KKR
Sbjct: 224 YRIDITAPDYYKAEEVLEDIAEEILRVIKEAGGEATLIRKEKRI-----KKVKKR 273

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,544,856
Number of Sequences: 369166
Number of extensions: 1648410
Number of successful extensions: 5855
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5843
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00286

  1. Dr_sW_028_F20
  2. Dr_sW_007_O12