Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_F20
(283 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6ZWX6|IF2A_MOUSE Eukaryotic translation initiation fact... 76 2e-14
sp|P05198|IF2A_HUMAN Eukaryotic translation initiation fact... 74 1e-13
sp|P41374|IF2A_DROME Eukaryotic translation initiation fact... 55 5e-08
sp|P56286|IF2A_SCHPO Eukaryotic translation initiation fact... 50 2e-06
sp|P20459|IF2A_YEAST Eukaryotic translation initiation fact... 49 5e-06
sp|Q99161|SEC62_YARLI Translocation protein SEC62 34 0.091
sp|Q8IUD2|RB6I2_HUMAN RAB6 interacting protein 2 (ERC prote... 32 0.59
sp|Q8U1I5|YC22_PYRFU Hypothetical UPF0330 protein PF1222 31 0.77
sp|Q5JHN1|RAD50_PYRKO DNA double-strand break repair rad50 ... 30 2.3
sp|Q811U3|RB6I2_RAT RAB6 interacting protein 2 (ERC protein... 29 2.9
>sp|Q6ZWX6|IF2A_MOUSE Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF-2alpha) (EIF-2A)
sp|P68101|IF2A_RAT Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF-2alpha) (EIF-2A)
sp|P68102|IF2A_BOVIN Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF-2alpha) (EIF-2A)
Length = 315
Score = 76.3 bits (186), Expect = 2e-14
Identities = 37/75 (49%), Positives = 53/75 (70%)
Frame = +1
Query: 1 LEIKINLIQSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHD 180
+ IKINLI P+++MT TL++ +GLS LN+A+ VI+E IE K+G F + +PKVV+D D
Sbjct: 223 MPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTD 282
Query: 181 EQNLKKRLAELEEAN 225
E L ++L LE N
Sbjct: 283 ETELARQLERLEREN 297
>sp|P05198|IF2A_HUMAN Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic
translation initiation factor 2 alpha subunit)
(eIF-2-alpha) (EIF-2alpha) (EIF-2A)
Length = 315
Score = 73.6 bits (179), Expect = 1e-13
Identities = 35/75 (46%), Positives = 53/75 (70%)
Frame = +1
Query: 1 LEIKINLIQSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHD 180
+ IKINLI P+++MT TL++ +GLS L++A+ VI+E IE K+G F + +PKVV+D D
Sbjct: 223 MPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTD 282
Query: 181 EQNLKKRLAELEEAN 225
E L +++ LE N
Sbjct: 283 ETELARQMERLEREN 297
>sp|P41374|IF2A_DROME Eukaryotic translation initiation factor 2 alpha subunit
(eIF-2-alpha)
Length = 341
Score = 55.1 bits (131), Expect = 5e-08
Identities = 31/75 (41%), Positives = 40/75 (53%)
Frame = +1
Query: 1 LEIKINLIQSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHD 180
L I+INLI P ++MT T K GL L AI IR G FK++ PK+V+ D
Sbjct: 221 LPIRINLIAPPLYVMTTSTTKKTDGLKALEVAIEHIRAKTSEYDGEFKVIMAPKLVTAID 280
Query: 181 EQNLKKRLAELEEAN 225
E +L +RL E N
Sbjct: 281 EADLARRLERAEAEN 295
>sp|P56286|IF2A_SCHPO Eukaryotic translation initiation factor 2 alpha subunit
(eIF-2-alpha)
Length = 306
Score = 49.7 bits (117), Expect = 2e-06
Identities = 25/73 (34%), Positives = 40/73 (54%)
Frame = +1
Query: 7 IKINLIQSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQ 186
IK+ L+ P +++ LDK GL L EAI I ++I + G+ + +PK VS+ DE
Sbjct: 219 IKVKLVAPPLYVLLTNALDKSLGLKKLEEAIGAIEKSITASNGTCTVKMKPKAVSETDEL 278
Query: 187 NLKKRLAELEEAN 225
L + + E+ N
Sbjct: 279 ELADLMKKFEKEN 291
>sp|P20459|IF2A_YEAST Eukaryotic translation initiation factor 2 alpha subunit
(eIF-2-alpha)
Length = 304
Score = 48.5 bits (114), Expect = 5e-06
Identities = 25/76 (32%), Positives = 43/76 (56%)
Frame = +1
Query: 1 LEIKINLIQSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHD 180
+++K+ L+ +P +++T Q LDK+KG+ L AI I E I G + PK V+ +
Sbjct: 216 MQVKVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATE 275
Query: 181 EQNLKKRLAELEEANN 228
+ L + L E +E +N
Sbjct: 276 DAEL-QALLESKELDN 290
>sp|Q99161|SEC62_YARLI Translocation protein SEC62
Length = 396
Score = 34.3 bits (77), Expect = 0.091
Identities = 19/68 (27%), Positives = 35/68 (51%)
Frame = +1
Query: 25 QSPQFMMTCQTLDKEKGLSTLNEAIRVIREAIESKKGSFKLLEQPKVVSDHDEQNLKKRL 204
Q P L+ E+ + L+E IR + I+ K + K ++ + V+D + + KR+
Sbjct: 328 QYPPDKQAAIKLENEQAQAKLDERIRELAAQIQDKTNANKTGDKIEEVADKENKEAPKRI 387
Query: 205 AELEEANN 228
LE+AN+
Sbjct: 388 VTLEDAND 395
>sp|Q8IUD2|RB6I2_HUMAN RAB6 interacting protein 2 (ERC protein 1)
Length = 1116
Score = 31.6 bits (70), Expect = 0.59
Identities = 24/90 (26%), Positives = 44/90 (48%), Gaps = 18/90 (20%)
Frame = +1
Query: 4 EIKINLIQSPQFMMTCQTLDKEKGLSTLNE--------------AIRVIREAIESKKGSF 141
E K+N++Q + Q DKEK +S+L E A+ + EA+ K+ +
Sbjct: 570 ERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALAEKERTI 629
Query: 142 KLLEQPKVVSDHDEQ----NLKKRLAELEE 219
+ L++ + + ++Q N KK L +L+E
Sbjct: 630 ERLKEQRDRDEREKQEEIDNYKKDLKDLKE 659
>sp|Q8U1I5|YC22_PYRFU Hypothetical UPF0330 protein PF1222
Length = 78
Score = 31.2 bits (69), Expect = 0.77
Identities = 18/50 (36%), Positives = 34/50 (68%), Gaps = 1/50 (2%)
Frame = +1
Query: 70 KGLSTLNEAIRVIREAIESKKGSFKLLEQP-KVVSDHDEQNLKKRLAELE 216
KG + +E V+RE ++ +KG+ ++L++ ++S+ + Q +KKRL ELE
Sbjct: 19 KGNKSFSE---VLRELLKERKGNKEVLKRIFGILSEEEYQEVKKRLKELE 65
>sp|Q5JHN1|RAD50_PYRKO DNA double-strand break repair rad50 ATPase
Length = 883
Score = 29.6 bits (65), Expect = 2.3
Identities = 21/56 (37%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = +1
Query: 52 QTLDKEKGLSTLNEAIRVIREAIES-KKGSFKLLEQPKVVSDHDEQNLKKRLAELE 216
+TL KE+ L L E + IRE E K+ + LE+ ++ + LKK+LA LE
Sbjct: 489 KTLKKERELFALKEVLEQIRETEEKLKEYDLEKLEE----ANEKAEELKKKLAGLE 540
>sp|Q811U3|RB6I2_RAT RAB6 interacting protein 2 (ERC protein 1) (ERC1) (CAZ-associated
structural protein 2) (CAST2)
Length = 948
Score = 29.3 bits (64), Expect = 2.9
Identities = 23/90 (25%), Positives = 43/90 (47%), Gaps = 18/90 (20%)
Frame = +1
Query: 4 EIKINLIQSPQFMMTCQTLDKEKGLSTLNE--------------AIRVIREAIESKKGSF 141
E K+N++Q + Q DKEK +S+L E A+ + EA+ K+ +
Sbjct: 542 ERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALADKERTI 601
Query: 142 KLLEQPKVVSDHDEQ----NLKKRLAELEE 219
+ L++ + + ++Q KK L +L+E
Sbjct: 602 ERLKEQRDRDEREKQEEIDTYKKDLKDLKE 631
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.310 0.128 0.332
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,266,415
Number of Sequences: 369166
Number of extensions: 494675
Number of successful extensions: 1485
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1485
length of database: 68,354,980
effective HSP length: 63
effective length of database: 56,716,675
effective search space used: 1701500250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)