Planaria EST Database

DrC_00247

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00247
         (988 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75746|CMC1_HUMAN  Calcium-binding mitochondrial carrier ...    49   2e-05
sp|Q8BH59|CMC1_MOUSE  Calcium-binding mitochondrial carrier ...    47   6e-05
sp|Q9QXX4|CMC2_MOUSE  Calcium-binding mitochondrial carrier ...    47   8e-05
sp|Q9UJS0|CMC2_HUMAN  Calcium-binding mitochondrial carrier ...    46   2e-04
sp|Q8HXW2|CMC2_MACFA  Calcium-binding mitochondrial carrier ...    46   2e-04
sp|Q9VA73|CMC1_DROME  Calcium-binding mitochondrial carrier ...    44   7e-04
sp|Q21153|CMC1_CAEEL  Probable calcium-binding mitochondrial...    43   0.002
sp|P36609|NCS2_CAEEL  Neuronal calcium sensor 2 (NCS-2)            39   0.017
sp|P21457|RECO_BOVIN  Recoverin (p26)                              38   0.039
sp|P34057|RECO_MOUSE  Recoverin (Cancer associated retinopat...    37   0.066

>sp|O75746|CMC1_HUMAN Calcium-binding mitochondrial carrier protein Aralar1
           (Mitochondrial aspartate glutamate carrier 1) (Solute
           carrier family 25 member 12)
          Length = 678

 Score = 48.9 bits (115), Expect = 2e-05
 Identities = 57/245 (23%), Positives = 104/245 (42%), Gaps = 13/245 (5%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKR 232
           ++ LI++ +++   +V+  P   F +AF++FD +G+GE+    F+ V+ I   G  +   
Sbjct: 68  KDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEV---TFENVKEIF--GQTIIHH 122

Query: 233 HRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEFN-RYSPE 409
           H         I   ++    +  FG   +K L   EF+ F + LQ E     F  +   +
Sbjct: 123 H---------IPFNWDCEFIRLHFGHNRKKHLNYTEFTQFLQELQLEHARQAFALKDKSK 173

Query: 410 NGIIKETDFADSLLMYADFHSTKKKKFLRRVRQKFNDENSIG---------ISFDDYFAF 562
           +G+I   DF+D ++       T            F +EN +          +SF  + AF
Sbjct: 174 SGMISGLDFSDIMVTIRSHMLT-----------PFVEENLVSAAGGSISHQVSFSYFNAF 222

Query: 563 SQILKSISDIDTALTFYNLAGAPIDQATLKH--VSKAVANVDLNDHLIDIVFTLFD-ENN 733
           + +L ++  +     +  LAG   D    K      A+    +    IDI++ L D  N 
Sbjct: 223 NSLLNNMELV--RKIYSTLAGTRKDVEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNA 280

Query: 734 DGQLS 748
            G+L+
Sbjct: 281 SGRLT 285

>sp|Q8BH59|CMC1_MOUSE Calcium-binding mitochondrial carrier protein Aralar1
           (Mitochondrial aspartate glutamate carrier 1) (Solute
           carrier family 25 member 12)
          Length = 677

 Score = 47.4 bits (111), Expect = 6e-05
 Identities = 57/245 (23%), Positives = 104/245 (42%), Gaps = 13/245 (5%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKR 232
           ++ LI++ +++   +V+  P   F +AF++FD +G+GE+    F+ V+ I   G  +   
Sbjct: 68  KDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEV---TFENVKEIF--GQTIIHH 122

Query: 233 HRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEFN-RYSPE 409
           H         I   ++    +  FG   +K L   EF+ F + LQ E     F  +   +
Sbjct: 123 H---------IPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLEHARQAFALKDKSK 173

Query: 410 NGIIKETDFADSLLMYADFHSTKKKKFLRRVRQKFNDENSIG---------ISFDDYFAF 562
           +G+I   DF+D ++       T            F +EN +          +SF  + AF
Sbjct: 174 SGMISGLDFSDVMVTIRSHMLT-----------PFVEENLVSAAGGGTSHQVSFSYFNAF 222

Query: 563 SQILKSISDIDTALTFYNLAGAPIDQATLKH--VSKAVANVDLNDHLIDIVFTLFD-ENN 733
           + +L ++  +     +  LAG   D    K      A+    +    IDI++ L D  N 
Sbjct: 223 NSLLNNMELV--RKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNA 280

Query: 734 DGQLS 748
            G+L+
Sbjct: 281 SGRLT 285

>sp|Q9QXX4|CMC2_MOUSE Calcium-binding mitochondrial carrier protein Aralar2
           (Mitochondrial aspartate glutamate carrier 2) (Solute
           carrier family 25 member 13) (Citrin)
          Length = 676

 Score = 47.0 bits (110), Expect = 8e-05
 Identities = 37/134 (27%), Positives = 63/134 (47%), Gaps = 1/134 (0%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKR 232
           ++ LI+F ++V   +V+  P   F +AF++FD  G GE+  E+   V+ I   G     +
Sbjct: 69  KDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFED---VKQIF--GQTTIHQ 123

Query: 233 HRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEF-NRYSPE 409
           H         I   ++S   +  FGK  ++ LT  EF+ F   +Q E     F  R + +
Sbjct: 124 H---------IPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNAK 174

Query: 410 NGIIKETDFADSLL 451
            G +   DF D ++
Sbjct: 175 TGKVSAIDFRDIMV 188

>sp|Q9UJS0|CMC2_HUMAN Calcium-binding mitochondrial carrier protein Aralar2
           (Mitochondrial aspartate glutamate carrier 2) (Solute
           carrier family 25 member 13) (Citrin)
          Length = 675

 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 36/134 (26%), Positives = 60/134 (44%), Gaps = 1/134 (0%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKR 232
           ++ LI+F ++V   +V+  P   F +AF++FD  G GE+  E+   V      G     +
Sbjct: 69  KDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQV-----FGQTTIHQ 123

Query: 233 HRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEF-NRYSPE 409
           H         I   ++S   +  FGK  ++ LT  EF+ F   +Q E     F  R +  
Sbjct: 124 H---------IPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNAR 174

Query: 410 NGIIKETDFADSLL 451
            G +   DF D ++
Sbjct: 175 TGRVTAIDFRDIMV 188

>sp|Q8HXW2|CMC2_MACFA Calcium-binding mitochondrial carrier protein Aralar2
           (Mitochondrial aspartate glutamate carrier 2) (Solute
           carrier family 25 member 13) (Citrin)
          Length = 674

 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 36/134 (26%), Positives = 60/134 (44%), Gaps = 1/134 (0%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKR 232
           ++ LI+F ++V   +V+  P   F +AF++FD  G GE+  E+   V      G     +
Sbjct: 69  KDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQV-----FGQTTIHQ 123

Query: 233 HRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEF-NRYSPE 409
           H         I   ++S   +  FGK  ++ LT  EF+ F   +Q E     F  R +  
Sbjct: 124 H---------IPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNAS 174

Query: 410 NGIIKETDFADSLL 451
            G +   DF D ++
Sbjct: 175 TGRVTAIDFRDIMV 188

>sp|Q9VA73|CMC1_DROME Calcium-binding mitochondrial carrier Aralar1
          Length = 695

 Score = 43.9 bits (102), Expect = 7e-04
 Identities = 58/278 (20%), Positives = 115/278 (41%), Gaps = 16/278 (5%)
 Frame = +2

Query: 53  ENALINFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEF-DVVRSIIRSGSPMGK 229
           ++ LI+FS++     ++ TP   +  AF++FD  G+G +   +F DVV+          +
Sbjct: 87  KDGLISFSEFQAFEGLLCTPDALYRTAFQLFDRKGNGTVSYADFADVVQKT--------E 138

Query: 230 RHRDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEFNRYSPE 409
            H       + I    +    K +FG + ++ +   EF+       +E  M  F    P 
Sbjct: 139 LH-------SKIPFSLDGPFIKRYFGDKKQRLINYAEFTQLLHDFHEEHAMEAFRSKDPA 191

Query: 410 -NGIIKETDFADSLLMYADFHSTKKKKFLRRVRQKF-NDENSIGISFDDYFAFSQILKSI 583
             G I   DF D ++      + K+      VR    +      +SF  + AF+ +L ++
Sbjct: 192 GTGFISPLDFQDIIV------NVKRHLLTPGVRDNLVSVTEGHKVSFPYFIAFTSLLNNM 245

Query: 584 SDIDTALTFYNLAGAPIDQATLKHVSKAVANVDLNDHL-IDIVFTL---------FDENN 733
            ++   +  +   G+  D  T   +  A   +     L IDI+F L          D ++
Sbjct: 246 -ELIKQVYLHATEGSRTDMITKDQILLAAQTMSQITPLEIDILFHLAGAVHQAGRIDYSD 304

Query: 734 DGQLSNKEFIMVMKQRL--FRGLDRPMD-TGFIRLMSA 838
              ++ + +   M  RL   + ++ P D + FI+++ +
Sbjct: 305 LSNIAPEHYTKHMTHRLAEIKAVESPADRSAFIQVLES 342

>sp|Q21153|CMC1_CAEEL Probable calcium-binding mitochondrial carrier K02F3.2
          Length = 702

 Score = 42.7 bits (99), Expect = 0.002
 Identities = 56/254 (22%), Positives = 100/254 (39%), Gaps = 12/254 (4%)
 Frame = +2

Query: 65  INFSDYVFLLTVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKRHRDN 244
           I+F ++     ++ +P   +  AF +FD N    +  +EF+   ++IR   P+  +  D 
Sbjct: 113 ISFEEFCAFEALLCSPDALYLTAFELFDRNASDTISCDEFE---AVIRHTQPLHDQDFD- 168

Query: 245 IMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMMLEFNRYSPE-NGII 421
                     F+S   K +FG   ++ +  + F        +E  +  F RY    NG I
Sbjct: 169 ----------FSSEFIKRYFGADKQRNVNYHSFCQLLHDFYEEQGIQAFKRYDKNGNGTI 218

Query: 422 KETDFADSLLMYADFHSTKKKKFLRRVRQKFNDENSIGIS---------FDDYFAFSQIL 574
              DF   +       +T K   L      F   N I +S         F  Y AF+ +L
Sbjct: 219 SSLDFQQIM-------TTVKGHLL----TDFVRHNLIAVSGGASGHKVTFPYYAAFNSLL 267

Query: 575 KSISDIDTALTFYNLAGAPIDQATLKHVSKAVANVDLNDHLIDIVFTLFDENNDGQ--LS 748
             +  I      Y      I ++ +   S+ +     N + ++I+F L + N+ G+  L 
Sbjct: 268 AKMELIKRV---YVSTTRKIGKSKIFRFSETIKK--YNKNKVEILFHLSELNHPGRKTLC 322

Query: 749 NKEFIMVMKQRLFR 790
            K+   +  +RL R
Sbjct: 323 LKDIQAIDPERLKR 336

>sp|P36609|NCS2_CAEEL Neuronal calcium sensor 2 (NCS-2)
          Length = 190

 Score = 39.3 bits (90), Expect = 0.017
 Identities = 28/116 (24%), Positives = 57/116 (49%), Gaps = 11/116 (9%)
 Frame = +2

Query: 56  NALINFSDYVFLLTVIS--TPQKQFEIAFRMFDLNGDGELDVEE-FDVVRSIIRSGSPMG 226
           +  I+F +++  + V S  TP+++ E AFRM+D++G+G +D +E   ++ +I     P  
Sbjct: 79  SGFIDFKEFLLAINVTSSGTPEQKLEWAFRMYDIDGNGTIDEKEMIKIIEAIYEMLGPEV 138

Query: 227 KRHRDN--------IMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQK 370
            +  D+        I     ++N    +L ++  G   +K+L    F I    ++K
Sbjct: 139 TKSADDSPRKRAKMIFEKMDVNNDKELTLKEFVDGCLADKEL----FQILTNEVKK 190

>sp|P21457|RECO_BOVIN Recoverin (p26)
          Length = 202

 Score = 38.1 bits (87), Expect = 0.039
 Identities = 31/110 (28%), Positives = 56/110 (50%), Gaps = 3/110 (2%)
 Frame = +2

Query: 65  INFSDYVFLLTVISTPQ--KQFEIAFRMFDLNGDGELDVEE-FDVVRSIIRSGSPMGKRH 235
           ++F +YV  L + S  +  ++ E AF ++D++G+G +   E  ++V +I +  SP   +H
Sbjct: 81  LDFKEYVIALHMTSAGKTNQKLEWAFSLYDVDGNGTISKNEVLEIVTAIFKMISPEDTKH 140

Query: 236 RDNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMML 385
               +  +  +    +     FFGK+ + KLT  EF I      KEI+ L
Sbjct: 141 ----LPEDENTPEKRAEKIWGFFGKKDDDKLTEKEF-IEGTLANKEILRL 185

>sp|P34057|RECO_MOUSE Recoverin (Cancer associated retinopathy protein) (CAR protein) (23
           kDa photoreceptor cell-specific protein)
          Length = 202

 Score = 37.4 bits (85), Expect = 0.066
 Identities = 31/109 (28%), Positives = 52/109 (47%), Gaps = 2/109 (1%)
 Frame = +2

Query: 65  INFSDYVFLL--TVISTPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKRHR 238
           ++F +YV  L  T    P ++ E AF ++D++G+G +   E   V  I+ +   M K   
Sbjct: 81  LDFKEYVIALHMTTAGKPTQKLEWAFSLYDVDGNGTISKNE---VLEIVMAIFKMIKPED 137

Query: 239 DNIMTGNSISNGFNSSLSKYFFGKRGEKKLTINEFSIFQRRLQKEIMML 385
             ++  +  +    +     FFGK+ + KLT  EF I      KEI+ L
Sbjct: 138 VKLLPDDENTPEKRAEKIWAFFGKKEDDKLTEEEF-IEGTLANKEILRL 185


 Score = 33.1 bits (74), Expect = 1.2
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +2

Query: 104 STPQKQFEIAFRMFDLNGDGELDVEEFDVVRSIIRSGSPMGK 229
           S P+   +  FR FD N DG LD +E+ +   +  +G P  K
Sbjct: 60  SDPKAYAQHVFRSFDANSDGTLDFKEYVIALHMTTAGKPTQK 101

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 107,938,908
Number of Sequences: 369166
Number of extensions: 2160901
Number of successful extensions: 6516
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6482
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10383318715
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00247

  1. Dr_sW_006_P10
  2. Dr_sW_003_M01
  3. Dr_sW_028_G11