Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_G11
(858 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75340|PDCD6_HUMAN Programmed cell death protein 6 (Prob... 37 0.054
sp|P81660|TNNC2_ANGAN Troponin C, skeletal muscle (TNC) 37 0.054
sp|P12815|PDCD6_MOUSE Programmed cell death protein 6 (Prob... 37 0.092
sp|P64173|FUMC_STAAN Fumarate hydratase class II (Fumarase ... 36 0.16
sp|Q8VBV8|GUC1B_MOUSE Guanylyl cyclase-activating protein 2... 35 0.27
sp|Q21153|CMC1_CAEEL Probable calcium-binding mitochondrial... 35 0.27
sp|Q6G884|FUMC_STAAS Fumarate hydratase class II (Fumarase ... 35 0.35
sp|O75746|CMC1_HUMAN Calcium-binding mitochondrial carrier ... 34 0.46
sp|P37236|FREQ_DROME Frequenin-1 (d-FRQ) 33 1.0
sp|Q59526|PARE_MYCGA Topoisomerase IV subunit B (TopIIB) 33 1.0
>sp|O75340|PDCD6_HUMAN Programmed cell death protein 6 (Probable calcium-binding protein
ALG-2)
Length = 191
Score = 37.4 bits (85), Expect = 0.054
Identities = 39/148 (26%), Positives = 68/148 (45%), Gaps = 8/148 (5%)
Frame = +3
Query: 279 NGIIKETDFADSLL--MYADFHSTKKKKFLRRVRQKFNDENSIGISFDDYFAFSQILKSI 452
+G+I +T+ +L + F+ +R + F+ EN G++F + F+ + K I
Sbjct: 40 SGVISDTELQQALSNGTWTPFNPVT----VRSIISMFDRENKAGVNFSE---FTGVWKYI 92
Query: 453 SDIDTALTFYNLAGAP-IDQATLKHVSKAVANVDLNDHLIDIVFTLFDENNDGQLSNKEF 629
+D Y+ + ID+ LK L+D DI+ FD GQ++ +F
Sbjct: 93 TDWQNVFRTYDRDNSGMIDKNELKQALSGFG-YRLSDQFHDILIRKFDRQGRGQIAFDDF 151
Query: 630 IM--VMKQRL---FRGLDRPMDTGFIRL 698
I ++ QRL FR D D G+I++
Sbjct: 152 IQGCIVLQRLTDIFRRYDTDQD-GWIQV 178
>sp|P81660|TNNC2_ANGAN Troponin C, skeletal muscle (TNC)
Length = 160
Score = 37.4 bits (85), Expect = 0.054
Identities = 20/47 (42%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +3
Query: 3 FRMFDLNGDGELDVEEF-DVVRSIIRSGSPMGKRHRDNIMTGNSISN 140
FR+FD NGDG +D EEF +++RS SG P+ + D +M +N
Sbjct: 100 FRVFDKNGDGFIDREEFGEILRS---SGEPVSEEEIDELMADGDKNN 143
>sp|P12815|PDCD6_MOUSE Programmed cell death protein 6 (Probable calcium-binding protein
ALG-2) (PMP41) (ALG-257)
Length = 191
Score = 36.6 bits (83), Expect = 0.092
Identities = 34/119 (28%), Positives = 56/119 (47%), Gaps = 6/119 (5%)
Frame = +3
Query: 360 LRRVRQKFNDENSIGISFDDYFAFSQILKSISDIDTALTFYNLAGAP-IDQATLKHVSKA 536
+R + F+ EN G++F + F+ + K I+D Y+ + ID+ LK
Sbjct: 65 VRSIISMFDRENKAGVNFSE---FTGVWKYITDWQNVFRTYDRDNSGMIDKNELKQALSG 121
Query: 537 VANVDLNDHLIDIVFTLFDENNDGQLSNKEFIM--VMKQRL---FRGLDRPMDTGFIRL 698
L+D DI+ FD GQ++ +FI ++ QRL FR D D G+I++
Sbjct: 122 FG-YRLSDQFHDILIRKFDRQGRGQIAFDDFIQGCIVLQRLTDIFRRYDTDQD-GWIQV 178
>sp|P64173|FUMC_STAAN Fumarate hydratase class II (Fumarase C)
sp|P64172|FUMC_STAAM Fumarate hydratase class II (Fumarase C)
Length = 461
Score = 35.8 bits (81), Expect = 0.16
Identities = 28/108 (25%), Positives = 44/108 (40%)
Frame = +3
Query: 423 FAFSQILKSISDIDTALTFYNLAGAPIDQATLKHVSKAVANVDLNDHLIDIVFTLFDENN 602
+A QIL D L + + V VAN+ L DH ID D+ N
Sbjct: 74 YACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQIDESIHPNDDVN 133
Query: 603 DGQLSNKEFIMVMKQRLFRGLDRPMDTGFIRLMSAVTKCSRDTVISVI 746
+ Q SN F M L++ ++ ++ ++L+ K D S+I
Sbjct: 134 ESQSSNDTFPTAMHVALYQEVETKLEPA-LKLLRNTLKEKEDKFESII 180
>sp|Q8VBV8|GUC1B_MOUSE Guanylyl cyclase-activating protein 2 (GCAP 2) (Guanylate cyclase
activator 1B)
Length = 201
Score = 35.0 bits (79), Expect = 0.27
Identities = 33/136 (24%), Positives = 57/136 (41%), Gaps = 6/136 (4%)
Frame = +3
Query: 243 IMMLEFNRYSPENGIIKETDFADSLLMYADFHSTKKKKFLRRVRQ-----KFNDENSIGI 407
+ M EF R+ G + + + +S+ D + FL V + + E+ +
Sbjct: 37 LFMHEFKRFFKVTGNEEASQYVESMFRAFDKNGDNTIDFLEYVAALNLVLRGSLEHKLKW 96
Query: 408 SFDDYFAFSQ-ILKSISDIDTALTFYNLAGAPIDQATLKHVSKAVANVDLNDHLIDIVFT 584
+F Y + + +D Y L A + L+H + + + ++D +F
Sbjct: 97 TFKIYDKDRNGCIDRLELLDIVEAIYKLKKACRAELDLEHQGQLLTP----EEVVDRIFL 152
Query: 585 LFDENNDGQLSNKEFI 632
L DEN DGQLS EFI
Sbjct: 153 LVDENGDGQLSLTEFI 168
>sp|Q21153|CMC1_CAEEL Probable calcium-binding mitochondrial carrier K02F3.2
Length = 702
Score = 35.0 bits (79), Expect = 0.27
Identities = 52/231 (22%), Positives = 89/231 (38%), Gaps = 12/231 (5%)
Frame = +3
Query: 3 FRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKRHRDNIMTGNSISNGFNSSLSKYFFGKR 182
F +FD N + +EF+ ++IR P+ + D F+S K +FG
Sbjct: 136 FELFDRNASDTISCDEFE---AVIRHTQPLHDQDFD-----------FSSEFIKRYFGAD 181
Query: 183 GEKKLTINEFSIFQRRLQKEIMMLEFNRYSPE-NGIIKETDFADSLLMYADFHSTKKKKF 359
++ + + F +E + F RY NG I DF + +T K
Sbjct: 182 KQRNVNYHSFCQLLHDFYEEQGIQAFKRYDKNGNGTISSLDFQQIM-------TTVKGHL 234
Query: 360 LRRVRQKFNDENSIGIS---------FDDYFAFSQILKSISDIDTALTFYNLAGAPIDQA 512
L F N I +S F Y AF+ +L + I Y I ++
Sbjct: 235 L----TDFVRHNLIAVSGGASGHKVTFPYYAAFNSLLAKMELIKRV---YVSTTRKIGKS 287
Query: 513 TLKHVSKAVANVDLNDHLIDIVFTLFDENNDGQ--LSNKEFIMVMKQRLFR 659
+ S+ + N + ++I+F L + N+ G+ L K+ + +RL R
Sbjct: 288 KIFRFSETIKK--YNKNKVEILFHLSELNHPGRKTLCLKDIQAIDPERLKR 336
>sp|Q6G884|FUMC_STAAS Fumarate hydratase class II (Fumarase C)
sp|Q8NVV1|FUMC_STAAW Fumarate hydratase class II (Fumarase C)
Length = 461
Score = 34.7 bits (78), Expect = 0.35
Identities = 28/108 (25%), Positives = 43/108 (39%)
Frame = +3
Query: 423 FAFSQILKSISDIDTALTFYNLAGAPIDQATLKHVSKAVANVDLNDHLIDIVFTLFDENN 602
+A QIL D L + + V VAN+ L DH ID D+ N
Sbjct: 74 YACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQIDESIHPNDDVN 133
Query: 603 DGQLSNKEFIMVMKQRLFRGLDRPMDTGFIRLMSAVTKCSRDTVISVI 746
Q SN F M L++ ++ ++ ++L+ K D S+I
Sbjct: 134 KSQSSNDTFPTAMHVALYQEVETKLEPA-LKLLRNTLKEKEDKFDSII 180
>sp|O75746|CMC1_HUMAN Calcium-binding mitochondrial carrier protein Aralar1
(Mitochondrial aspartate glutamate carrier 1) (Solute
carrier family 25 member 12)
Length = 678
Score = 34.3 bits (77), Expect = 0.46
Identities = 51/218 (23%), Positives = 88/218 (40%), Gaps = 13/218 (5%)
Frame = +3
Query: 3 FRMFDLNGDGELDVEEFDVVRSIIRSGSPMGKRHRDNIMTGNSISNGFNSSLSKYFFGKR 182
F++FD +G+GE+ F+ V+ I G + H I ++ + FG
Sbjct: 95 FQLFDKSGNGEV---TFENVKEIF--GQTIIHHH---------IPFNWDCEFIRLHFGHN 140
Query: 183 GEKKLTINEFSIFQRRLQKEIMMLEFN-RYSPENGIIKETDFADSLLMYADFHSTKKKKF 359
+K L EF+ F + LQ E F + ++G+I DF+D ++ T
Sbjct: 141 RKKHLNYTEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDIMVTIRSHMLT----- 195
Query: 360 LRRVRQKFNDENSIG---------ISFDDYFAFSQILKSISDIDTALTFYNLAGAPIDQA 512
F +EN + +SF + AF+ +L ++ + + LAG D
Sbjct: 196 ------PFVEENLVSAAGGSISHQVSFSYFNAFNSLLNNMELV--RKIYSTLAGTRKDVE 247
Query: 513 TLKH--VSKAVANVDLNDHLIDIVFTLFD-ENNDGQLS 617
K A+ + IDI++ L D N G+L+
Sbjct: 248 VTKEEFAQSAIRYGQVTPLEIDILYQLADLYNASGRLT 285
>sp|P37236|FREQ_DROME Frequenin-1 (d-FRQ)
Length = 187
Score = 33.1 bits (74), Expect = 1.0
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +3
Query: 573 IVFTLFDENNDGQLSNKEFIMVMKQRLFRGLDRPMDTGF 689
+VF +FDENNDG + +EFI + LD + F
Sbjct: 67 LVFRVFDENNDGSIEFEEFIRALSVTSKGNLDEKLQWAF 105
>sp|Q59526|PARE_MYCGA Topoisomerase IV subunit B (TopIIB)
Length = 633
Score = 33.1 bits (74), Expect = 1.0
Identities = 16/42 (38%), Positives = 27/42 (64%)
Frame = -3
Query: 316 NESAKSVSLIIPFSGEYRLNSNIMISFCSRR*NMENSLMVNF 191
NE+ KS+S IIPF G+Y I++ C + + E+ L+++F
Sbjct: 230 NENKKSISKIIPFKGDY---EKIVVDGCFQYTDNESELIISF 268
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,062,467
Number of Sequences: 369166
Number of extensions: 1899188
Number of successful extensions: 5782
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5016
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5759
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)