Planaria EST Database


DrC_00205

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00205
         (758 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O89079|COPE_MOUSE  Coatomer epsilon subunit (Epsilon-coat...   213   3e-55
sp|Q60445|COPE_CRIGR  Coatomer epsilon subunit (Epsilon-coat...   213   6e-55
sp|O14579|COPE_HUMAN  Coatomer epsilon subunit (Epsilon-coat...   211   2e-54
sp|Q28104|COPE_BOVIN  Coatomer epsilon subunit (Epsilon-coat...   211   2e-54
sp|O64748|COPE_ARATH  Probable coatomer epsilon subunit (Eps...   147   3e-35
sp|O62246|COPE_CAEEL  Probable coatomer epsilon subunit (Eps...   126   7e-29
sp|O74767|COPE_SCHPO  Probable coatomer epsilon subunit (Eps...    51   3e-06
sp|Q58741|YD45_METJA  Hypothetical protein MJ1345                  45   2e-04
sp|Q8ZBT8|SYA_YERPE  Alanyl-tRNA synthetase (Alanine--tRNA l...    36   0.098
sp|Q66E68|SYA_YERPS  Alanyl-tRNA synthetase (Alanine--tRNA l...    36   0.098
>sp|O89079|COPE_MOUSE Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
          Length = 308

 Score =  213 bits (543), Expect = 3e-55
 Identities = 103/224 (45%), Positives = 159/224 (70%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           R+ A+Y +    +  ++ E++  ++ ++DV +   LLMA +IY  +  P+ AL+ LH G 
Sbjct: 85  RMFAEYLASENQRDSIVLELDREMSRSVDVTNTTFLLMAASIYFHDQNPDAALRTLHQGD 144

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
             EC +M IQ L++++R+DLA+KELKKM + +ED  L+Q++ A VNL++GG+KL+EAY I
Sbjct: 145 GLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYI 204

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           +QEL +K  +PT ++ NGQA      G+ + AE +LQ+A+DK+   P+TL+N+IVLSQ L
Sbjct: 205 FQELADKC-SPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHL 263

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
           GKP E+TNR++SQLKD+H  HPF+K++ +KE +FDRL   Y  S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRAHPFIKEYQAKENDFDRLAMQYAPS 307
>sp|Q60445|COPE_CRIGR Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
           (LDLF)
          Length = 308

 Score =  213 bits (541), Expect = 6e-55
 Identities = 103/224 (45%), Positives = 159/224 (70%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           R+ A Y +    +  ++ E++  ++ ++DV +   LLMA ++Y  +  P+ AL+ LH G 
Sbjct: 85  RMFADYLATENRRDAIVVELDREMSRSVDVTNTTFLLMAASVYFHDQNPDAALRTLHQGD 144

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
           S EC +M IQ L++++R+DLA+KELKKM + +ED  L+Q++ A VNL++GG+KL+EAY I
Sbjct: 145 SLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYI 204

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           +QEL +K  +PT ++ NGQA      G+ + AE +LQ+A+DK+   P+TL+N+IVLSQ L
Sbjct: 205 FQELADKC-SPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHL 263

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
           GKP E+TNR++SQLKD+H  HPF+K++ +KE +FDRL   Y  S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRTHPFIKEYQAKENDFDRLALQYAPS 307
>sp|O14579|COPE_HUMAN Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
          Length = 308

 Score =  211 bits (537), Expect = 2e-54
 Identities = 101/224 (45%), Positives = 162/224 (72%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           R+ A Y +    +  ++ E++  ++ ++DV +   LLMA +IYL +  P+ AL+ LH G 
Sbjct: 85  RMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGD 144

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
           S EC +M +Q L++++R+DLA+KELK+M + +ED  L+Q++ A V+L+ GG+KL++AY I
Sbjct: 145 SLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYI 204

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           +QE+ +K  +PT ++ NGQA   +  G+ + AE +LQ+A+DK+   P+TLVN+IVLSQ L
Sbjct: 205 FQEMADKC-SPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHL 263

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
           GKP E+TNR++SQLKD+H +HPF+K++ +KE +FDRL+  Y  S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPS 307
>sp|Q28104|COPE_BOVIN Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
          Length = 308

 Score =  211 bits (537), Expect = 2e-54
 Identities = 99/224 (44%), Positives = 162/224 (72%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           R+ A+Y +    +  ++ E++  ++ ++DV +   LLMA +IY  +  P+ AL+ LH G 
Sbjct: 85  RMFAEYLASHSRRDAIVAELDREMSRSVDVTNTTFLLMAASIYFYDQNPDAALRTLHQGD 144

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
           S EC +M +Q L++++R+DLA+KELKKM + +ED  L+Q++ A V+L+ GG+KL++AY I
Sbjct: 145 SLECMAMTVQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVSLAAGGEKLQDAYYI 204

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           +QE+ +K  +PT ++ NGQA   +  G+ + AE +LQ+A+DK+   P+TL+N++VLSQ L
Sbjct: 205 FQEMADKC-SPTLLLLNGQAACHMAQGRWEAAEGVLQEALDKDSGHPETLINLVVLSQHL 263

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
           GKP E+TNR++SQLKD+H +HPF+K++ +KE +FDRL+  Y  S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRSHPFIKEYRAKENDFDRLVLQYAPS 307
>sp|O64748|COPE_ARATH Probable coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP)
          Length = 293

 Score =  147 bits (371), Expect = 3e-35
 Identities = 80/216 (37%), Positives = 135/216 (62%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           +LLA Y S  ++++  +  ++E L  +    +D   L+AG I++ E    + LK  H G 
Sbjct: 76  KLLAMYLSTPQNKESTISSLKEWLADSTIGNNDTLRLIAGIIFMHEEDYNETLKHTHAGG 135

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
           + +  ++ +Q  I+M+R + A+K+L+ M + +ED  L+Q++ A +NL++GG K++EAY I
Sbjct: 136 TMDLYALNVQIFIKMHRAEYAEKQLRVMQQIDEDHTLTQLASAWLNLAVGGSKIQEAYLI 195

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           +++  EKY   T +I NG+AV  + +G  DEAE +L +A++K+  DP+TL N++V S  +
Sbjct: 196 FEDFSEKYPM-TCLILNGKAVCCMQMGNFDEAETLLLEALNKDAKDPETLANLVVCSLHV 254

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDR 654
           GK    ++R +SQLK SHP H  VK   S E  F+R
Sbjct: 255 GKS---SSRHLSQLKLSHPEHILVKRVSSAEDNFER 287
>sp|O62246|COPE_CAEEL Probable coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP)
          Length = 292

 Score =  126 bits (316), Expect = 7e-29
 Identities = 73/220 (33%), Positives = 131/220 (59%)
 Frame = +1

Query: 7   RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
           R  A++ ++  ++K++L E++E +  + +++ +I  ++A TI  +    + A + +    
Sbjct: 72  RRYAEFRNNPAAKKKILAEVQEEV-ASRNIKSEIAAVLAATILNEADLSQDAFRAVSRFE 130

Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
             E ++  +  LI+MN+  LA  E+KKM + +ED  LSQ++ A+V       K+++A  I
Sbjct: 131 GLEARASKVFILIKMNKRKLAIGEVKKMNQIDEDATLSQLANALVTSFGASGKVKDALYI 190

Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
           Y E+ +KY   T++  +   V++L       AE +L+ A++++  D D L+N IV +Q+ 
Sbjct: 191 YSEMSDKYGRTTDLEMHQAVVSILTQDYA-AAEELLESALERDNKDADVLINSIVSAQLN 249

Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNN 666
            K  ++  RFISQLK  HPNHP+V DF  KEAEFDR+ ++
Sbjct: 250 EKDDDVVERFISQLKHEHPNHPWVIDFNEKEAEFDRVASD 289
>sp|O74767|COPE_SCHPO Probable coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP)
          Length = 288

 Score = 51.2 bits (121), Expect = 3e-06
 Identities = 33/125 (26%), Positives = 68/125 (54%), Gaps = 3/125 (2%)
 Frame = +1

Query: 184 SSSECQSMAIQALIQM--NRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGG-DKLRE 354
           S    +++A+Q  I +  ++++ A++ LK+ L+  +++++ Q++ + + +  GG +   +
Sbjct: 122 SVENLEAVALQVYIHLREHKIEAAEQTLKQALDWADEEIVLQLAQSWIKIVSGGVESYND 181

Query: 355 AYNIYQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVL 534
           A+ +++EL      P  M   G A A + L +P+EA   L+ A+D  PN  + L NM   
Sbjct: 182 AFYVFEELNGTDSNP--MTLTGMACADICLLRPEEALSSLKTALDSQPNYEEALSNMTTA 239

Query: 535 SQMLG 549
              LG
Sbjct: 240 ITDLG 244
>sp|Q58741|YD45_METJA Hypothetical protein MJ1345
          Length = 314

 Score = 45.1 bits (105), Expect = 2e-04
 Identities = 38/148 (25%), Positives = 70/148 (47%), Gaps = 5/148 (3%)
 Frame = +1

Query: 124 GTIYLQEMRPEQALKVLHNG---SSSECQSMAIQA--LIQMNRVDLAQKELKKMLETNED 288
           G I  +E + +++L+   N    +  +CQS+  +   L ++ R   A K LKK+ E N  
Sbjct: 154 GEILYREGKLKKSLECFDNALKINPKDCQSLLYKGEILFKLGRYGEALKCLKKVFERNNK 213

Query: 289 DVLSQISLAMVNLSLGGDKLREAYNIYQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEP 468
           D+ + + +  + + LG  +L +A    ++ L K      ++   + + L  LGK +EA  
Sbjct: 214 DIRALMYIIQILIYLG--RLNQALEYTKKAL-KLNPDDPLLYLYKGIILNKLGKYNEAIK 270

Query: 469 ILQDAMDKNPNDPDTLVNMIVLSQMLGK 552
                ++ NPN PD      +  + LGK
Sbjct: 271 YFDKVLEINPNIPDAWNGKAIALEKLGK 298

 Score = 45.1 bits (105), Expect = 2e-04
 Identities = 43/139 (30%), Positives = 64/139 (46%), Gaps = 5/139 (3%)
 Frame = +1

Query: 88  LDVEDDITLLMAGTIYLQEMRPEQALKVL-----HNGSSSECQSMAIQALIQMNRVDLAQ 252
           ++ +D  +LL  G I  +  R  +ALK L      N          IQ LI + R++ A 
Sbjct: 176 INPKDCQSLLYKGEILFKLGRYGEALKCLKKVFERNNKDIRALMYIIQILIYLGRLNQAL 235

Query: 253 KELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNIYQELLEKYQAPTNMIKNGQAVA 432
           +  KK L+ N DD L  +   ++   LG  K  EA   + ++LE          NG+A+A
Sbjct: 236 EYTKKALKLNPDDPLLYLYKGIILNKLG--KYNEAIKYFDKVLE-INPNIPDAWNGKAIA 292

Query: 433 LLGLGKPDEAEPILQDAMD 489
           L  LGK +EA      A+D
Sbjct: 293 LEKLGKINEAIECYNRALD 311
>sp|Q8ZBT8|SYA_YERPE Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
          Length = 875

 Score = 36.2 bits (82), Expect = 0.098
 Identities = 35/148 (23%), Positives = 76/148 (51%), Gaps = 7/148 (4%)
 Frame = +1

Query: 43   QKEVLREMEEILNGNL-DVEDDITLLMAGTIYL----QEMRPEQALKVLHNGSSSECQSM 207
            Q ++L+++  ++ G++ ++ D +  ++  +  L    Q+++ +QA +   + SSS     
Sbjct: 710  QSDLLQDVAHLVKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLIN 769

Query: 208  AIQALI-QMNRVDLAQKELKKMLETNEDDVLSQISLAMVNL-SLGGDKLREAYNIYQELL 381
             ++ L+ Q++ V+       KML T  DD+ +Q+  A++ L +   DK+     + ++L 
Sbjct: 770  GVKLLVSQLDNVE------PKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLT 823

Query: 382  EKYQAPTNMIKNGQAVALLGLGKPDEAE 465
             K +A   +    Q V   G G+PD A+
Sbjct: 824  GKVKAGELIADIAQQVGGKGGGRPDMAQ 851
>sp|Q66E68|SYA_YERPS Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
          Length = 875

 Score = 36.2 bits (82), Expect = 0.098
 Identities = 35/148 (23%), Positives = 76/148 (51%), Gaps = 7/148 (4%)
 Frame = +1

Query: 43   QKEVLREMEEILNGNL-DVEDDITLLMAGTIYL----QEMRPEQALKVLHNGSSSECQSM 207
            Q ++L+++  ++ G++ ++ D +  ++  +  L    Q+++ +QA +   + SSS     
Sbjct: 710  QSDLLQDVAHLVKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLIN 769

Query: 208  AIQALI-QMNRVDLAQKELKKMLETNEDDVLSQISLAMVNL-SLGGDKLREAYNIYQELL 381
             ++ L+ Q++ V+       KML T  DD+ +Q+  A++ L +   DK+     + ++L 
Sbjct: 770  GVKLLVSQLDNVE------PKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLT 823

Query: 382  EKYQAPTNMIKNGQAVALLGLGKPDEAE 465
             K +A   +    Q V   G G+PD A+
Sbjct: 824  GKVKAGELIADIAQQVGGKGGGRPDMAQ 851
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.314    0.132    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,255,965
Number of Sequences: 369166
Number of extensions: 1856379
Number of successful extensions: 3813
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3804
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)

Cluster detail

DrC_00205

  1. Dr_sW_006_B11
  2. Dr_sW_026_M03