Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_B11
(758 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O89079|COPE_MOUSE Coatomer epsilon subunit (Epsilon-coat... 213 3e-55
sp|Q60445|COPE_CRIGR Coatomer epsilon subunit (Epsilon-coat... 213 6e-55
sp|O14579|COPE_HUMAN Coatomer epsilon subunit (Epsilon-coat... 211 2e-54
sp|Q28104|COPE_BOVIN Coatomer epsilon subunit (Epsilon-coat... 211 2e-54
sp|O64748|COPE_ARATH Probable coatomer epsilon subunit (Eps... 147 3e-35
sp|O62246|COPE_CAEEL Probable coatomer epsilon subunit (Eps... 126 7e-29
sp|O74767|COPE_SCHPO Probable coatomer epsilon subunit (Eps... 51 3e-06
sp|Q58741|YD45_METJA Hypothetical protein MJ1345 45 2e-04
sp|Q8ZBT8|SYA_YERPE Alanyl-tRNA synthetase (Alanine--tRNA l... 36 0.098
sp|Q66E68|SYA_YERPS Alanyl-tRNA synthetase (Alanine--tRNA l... 36 0.098
>sp|O89079|COPE_MOUSE Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
Length = 308
Score = 213 bits (543), Expect = 3e-55
Identities = 103/224 (45%), Positives = 159/224 (70%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
R+ A+Y + + ++ E++ ++ ++DV + LLMA +IY + P+ AL+ LH G
Sbjct: 85 RMFAEYLASENQRDSIVLELDREMSRSVDVTNTTFLLMAASIYFHDQNPDAALRTLHQGD 144
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
EC +M IQ L++++R+DLA+KELKKM + +ED L+Q++ A VNL++GG+KL+EAY I
Sbjct: 145 GLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYI 204
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
+QEL +K +PT ++ NGQA G+ + AE +LQ+A+DK+ P+TL+N+IVLSQ L
Sbjct: 205 FQELADKC-SPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHL 263
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
GKP E+TNR++SQLKD+H HPF+K++ +KE +FDRL Y S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRAHPFIKEYQAKENDFDRLAMQYAPS 307
>sp|Q60445|COPE_CRIGR Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
(LDLF)
Length = 308
Score = 213 bits (541), Expect = 6e-55
Identities = 103/224 (45%), Positives = 159/224 (70%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
R+ A Y + + ++ E++ ++ ++DV + LLMA ++Y + P+ AL+ LH G
Sbjct: 85 RMFADYLATENRRDAIVVELDREMSRSVDVTNTTFLLMAASVYFHDQNPDAALRTLHQGD 144
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
S EC +M IQ L++++R+DLA+KELKKM + +ED L+Q++ A VNL++GG+KL+EAY I
Sbjct: 145 SLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYI 204
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
+QEL +K +PT ++ NGQA G+ + AE +LQ+A+DK+ P+TL+N+IVLSQ L
Sbjct: 205 FQELADKC-SPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHL 263
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
GKP E+TNR++SQLKD+H HPF+K++ +KE +FDRL Y S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRTHPFIKEYQAKENDFDRLALQYAPS 307
>sp|O14579|COPE_HUMAN Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
Length = 308
Score = 211 bits (537), Expect = 2e-54
Identities = 101/224 (45%), Positives = 162/224 (72%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
R+ A Y + + ++ E++ ++ ++DV + LLMA +IYL + P+ AL+ LH G
Sbjct: 85 RMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGD 144
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
S EC +M +Q L++++R+DLA+KELK+M + +ED L+Q++ A V+L+ GG+KL++AY I
Sbjct: 145 SLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYI 204
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
+QE+ +K +PT ++ NGQA + G+ + AE +LQ+A+DK+ P+TLVN+IVLSQ L
Sbjct: 205 FQEMADKC-SPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHL 263
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
GKP E+TNR++SQLKD+H +HPF+K++ +KE +FDRL+ Y S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPS 307
>sp|Q28104|COPE_BOVIN Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
Length = 308
Score = 211 bits (537), Expect = 2e-54
Identities = 99/224 (44%), Positives = 162/224 (72%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
R+ A+Y + + ++ E++ ++ ++DV + LLMA +IY + P+ AL+ LH G
Sbjct: 85 RMFAEYLASHSRRDAIVAELDREMSRSVDVTNTTFLLMAASIYFYDQNPDAALRTLHQGD 144
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
S EC +M +Q L++++R+DLA+KELKKM + +ED L+Q++ A V+L+ GG+KL++AY I
Sbjct: 145 SLECMAMTVQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVSLAAGGEKLQDAYYI 204
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
+QE+ +K +PT ++ NGQA + G+ + AE +LQ+A+DK+ P+TL+N++VLSQ L
Sbjct: 205 FQEMADKC-SPTLLLLNGQAACHMAQGRWEAAEGVLQEALDKDSGHPETLINLVVLSQHL 263
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNNYGVS 678
GKP E+TNR++SQLKD+H +HPF+K++ +KE +FDRL+ Y S
Sbjct: 264 GKPPEVTNRYLSQLKDAHRSHPFIKEYRAKENDFDRLVLQYAPS 307
>sp|O64748|COPE_ARATH Probable coatomer epsilon subunit (Epsilon-coat protein)
(Epsilon-COP)
Length = 293
Score = 147 bits (371), Expect = 3e-35
Identities = 80/216 (37%), Positives = 135/216 (62%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
+LLA Y S ++++ + ++E L + +D L+AG I++ E + LK H G
Sbjct: 76 KLLAMYLSTPQNKESTISSLKEWLADSTIGNNDTLRLIAGIIFMHEEDYNETLKHTHAGG 135
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
+ + ++ +Q I+M+R + A+K+L+ M + +ED L+Q++ A +NL++GG K++EAY I
Sbjct: 136 TMDLYALNVQIFIKMHRAEYAEKQLRVMQQIDEDHTLTQLASAWLNLAVGGSKIQEAYLI 195
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
+++ EKY T +I NG+AV + +G DEAE +L +A++K+ DP+TL N++V S +
Sbjct: 196 FEDFSEKYPM-TCLILNGKAVCCMQMGNFDEAETLLLEALNKDAKDPETLANLVVCSLHV 254
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDR 654
GK ++R +SQLK SHP H VK S E F+R
Sbjct: 255 GKS---SSRHLSQLKLSHPEHILVKRVSSAEDNFER 287
>sp|O62246|COPE_CAEEL Probable coatomer epsilon subunit (Epsilon-coat protein)
(Epsilon-COP)
Length = 292
Score = 126 bits (316), Expect = 7e-29
Identities = 73/220 (33%), Positives = 131/220 (59%)
Frame = +1
Query: 7 RLLAKYFSDSESQKEVLREMEEILNGNLDVEDDITLLMAGTIYLQEMRPEQALKVLHNGS 186
R A++ ++ ++K++L E++E + + +++ +I ++A TI + + A + +
Sbjct: 72 RRYAEFRNNPAAKKKILAEVQEEV-ASRNIKSEIAAVLAATILNEADLSQDAFRAVSRFE 130
Query: 187 SSECQSMAIQALIQMNRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNI 366
E ++ + LI+MN+ LA E+KKM + +ED LSQ++ A+V K+++A I
Sbjct: 131 GLEARASKVFILIKMNKRKLAIGEVKKMNQIDEDATLSQLANALVTSFGASGKVKDALYI 190
Query: 367 YQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVLSQML 546
Y E+ +KY T++ + V++L AE +L+ A++++ D D L+N IV +Q+
Sbjct: 191 YSEMSDKYGRTTDLEMHQAVVSILTQDYA-AAEELLESALERDNKDADVLINSIVSAQLN 249
Query: 547 GKPFEITNRFISQLKDSHPNHPFVKDFVSKEAEFDRLMNN 666
K ++ RFISQLK HPNHP+V DF KEAEFDR+ ++
Sbjct: 250 EKDDDVVERFISQLKHEHPNHPWVIDFNEKEAEFDRVASD 289
>sp|O74767|COPE_SCHPO Probable coatomer epsilon subunit (Epsilon-coat protein)
(Epsilon-COP)
Length = 288
Score = 51.2 bits (121), Expect = 3e-06
Identities = 33/125 (26%), Positives = 68/125 (54%), Gaps = 3/125 (2%)
Frame = +1
Query: 184 SSSECQSMAIQALIQM--NRVDLAQKELKKMLETNEDDVLSQISLAMVNLSLGG-DKLRE 354
S +++A+Q I + ++++ A++ LK+ L+ +++++ Q++ + + + GG + +
Sbjct: 122 SVENLEAVALQVYIHLREHKIEAAEQTLKQALDWADEEIVLQLAQSWIKIVSGGVESYND 181
Query: 355 AYNIYQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEPILQDAMDKNPNDPDTLVNMIVL 534
A+ +++EL P M G A A + L +P+EA L+ A+D PN + L NM
Sbjct: 182 AFYVFEELNGTDSNP--MTLTGMACADICLLRPEEALSSLKTALDSQPNYEEALSNMTTA 239
Query: 535 SQMLG 549
LG
Sbjct: 240 ITDLG 244
>sp|Q58741|YD45_METJA Hypothetical protein MJ1345
Length = 314
Score = 45.1 bits (105), Expect = 2e-04
Identities = 38/148 (25%), Positives = 70/148 (47%), Gaps = 5/148 (3%)
Frame = +1
Query: 124 GTIYLQEMRPEQALKVLHNG---SSSECQSMAIQA--LIQMNRVDLAQKELKKMLETNED 288
G I +E + +++L+ N + +CQS+ + L ++ R A K LKK+ E N
Sbjct: 154 GEILYREGKLKKSLECFDNALKINPKDCQSLLYKGEILFKLGRYGEALKCLKKVFERNNK 213
Query: 289 DVLSQISLAMVNLSLGGDKLREAYNIYQELLEKYQAPTNMIKNGQAVALLGLGKPDEAEP 468
D+ + + + + + LG +L +A ++ L K ++ + + L LGK +EA
Sbjct: 214 DIRALMYIIQILIYLG--RLNQALEYTKKAL-KLNPDDPLLYLYKGIILNKLGKYNEAIK 270
Query: 469 ILQDAMDKNPNDPDTLVNMIVLSQMLGK 552
++ NPN PD + + LGK
Sbjct: 271 YFDKVLEINPNIPDAWNGKAIALEKLGK 298
Score = 45.1 bits (105), Expect = 2e-04
Identities = 43/139 (30%), Positives = 64/139 (46%), Gaps = 5/139 (3%)
Frame = +1
Query: 88 LDVEDDITLLMAGTIYLQEMRPEQALKVL-----HNGSSSECQSMAIQALIQMNRVDLAQ 252
++ +D +LL G I + R +ALK L N IQ LI + R++ A
Sbjct: 176 INPKDCQSLLYKGEILFKLGRYGEALKCLKKVFERNNKDIRALMYIIQILIYLGRLNQAL 235
Query: 253 KELKKMLETNEDDVLSQISLAMVNLSLGGDKLREAYNIYQELLEKYQAPTNMIKNGQAVA 432
+ KK L+ N DD L + ++ LG K EA + ++LE NG+A+A
Sbjct: 236 EYTKKALKLNPDDPLLYLYKGIILNKLG--KYNEAIKYFDKVLE-INPNIPDAWNGKAIA 292
Query: 433 LLGLGKPDEAEPILQDAMD 489
L LGK +EA A+D
Sbjct: 293 LEKLGKINEAIECYNRALD 311
>sp|Q8ZBT8|SYA_YERPE Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 875
Score = 36.2 bits (82), Expect = 0.098
Identities = 35/148 (23%), Positives = 76/148 (51%), Gaps = 7/148 (4%)
Frame = +1
Query: 43 QKEVLREMEEILNGNL-DVEDDITLLMAGTIYL----QEMRPEQALKVLHNGSSSECQSM 207
Q ++L+++ ++ G++ ++ D + ++ + L Q+++ +QA + + SSS
Sbjct: 710 QSDLLQDVAHLVKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLIN 769
Query: 208 AIQALI-QMNRVDLAQKELKKMLETNEDDVLSQISLAMVNL-SLGGDKLREAYNIYQELL 381
++ L+ Q++ V+ KML T DD+ +Q+ A++ L + DK+ + ++L
Sbjct: 770 GVKLLVSQLDNVE------PKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLT 823
Query: 382 EKYQAPTNMIKNGQAVALLGLGKPDEAE 465
K +A + Q V G G+PD A+
Sbjct: 824 GKVKAGELIADIAQQVGGKGGGRPDMAQ 851
>sp|Q66E68|SYA_YERPS Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 875
Score = 36.2 bits (82), Expect = 0.098
Identities = 35/148 (23%), Positives = 76/148 (51%), Gaps = 7/148 (4%)
Frame = +1
Query: 43 QKEVLREMEEILNGNL-DVEDDITLLMAGTIYL----QEMRPEQALKVLHNGSSSECQSM 207
Q ++L+++ ++ G++ ++ D + ++ + L Q+++ +QA + + SSS
Sbjct: 710 QSDLLQDVAHLVKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLIN 769
Query: 208 AIQALI-QMNRVDLAQKELKKMLETNEDDVLSQISLAMVNL-SLGGDKLREAYNIYQELL 381
++ L+ Q++ V+ KML T DD+ +Q+ A++ L + DK+ + ++L
Sbjct: 770 GVKLLVSQLDNVE------PKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLT 823
Query: 382 EKYQAPTNMIKNGQAVALLGLGKPDEAE 465
K +A + Q V G G+PD A+
Sbjct: 824 GKVKAGELIADIAQQVGGKGGGRPDMAQ 851
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.314 0.132 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,255,965
Number of Sequences: 369166
Number of extensions: 1856379
Number of successful extensions: 3813
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3804
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)