Planaria EST Database


DrC_00186

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00186
         (526 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P68037|UB2L3_MOUSE  Ubiquitin-conjugating enzyme E2 L3 (U...   132   4e-31
sp|P52487|UBC84_DROME  Ubiquitin-conjugating enzyme E2-18 kD...   123   3e-28
sp|O14933|UB2L6_HUMAN  Ubiquitin-conjugating enzyme E2 L6 (U...   102   4e-22
sp|Q9QZU9|UB2L6_MOUSE  Ubiquitin-conjugating enzyme E2 L6 (U...    96   5e-20
sp|P63146|UBE2B_HUMAN  Ubiquitin-conjugating enzyme E2 B (Ub...    79   5e-15
sp|P52478|UBC1_CAEEL  Ubiquitin-conjugating enzyme E2 1 (Ubi...    76   4e-14
sp|Q9Z255|UBE2A_MOUSE  Ubiquitin-conjugating enzyme E2 A (Ub...    76   6e-14
sp|P25153|UBCD6_DROME  Ubiquitin-conjugating enzyme E2-17 kD...    74   2e-13
sp|Q9CQ37|UBE2T_MOUSE  Ubiquitin-conjugating enzyme E2 T (Ub...    74   2e-13
sp|P25865|UBC1_ARATH  Ubiquitin-conjugating enzyme E2-17 kDa...    73   5e-13
>sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
           (Ubiquitin carrier protein L3) (UbcM4)
 sp|P68036|UB2L3_HUMAN Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
           (Ubiquitin carrier protein L3) (UbcH7) (E2-F1) (L-UBC)
          Length = 154

 Score =  132 bits (333), Expect = 4e-31
 Identities = 54/121 (44%), Positives = 82/121 (67%)
 Frame = +2

Query: 50  FKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDE 229
           F+  + D +N+  W+GL+ P NPP+++ AF + + F   +PFKPP IT KT IYHPN+DE
Sbjct: 22  FRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDE 81

Query: 230 QGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
           +G VCL +I+A NWKP  + +Q+I+    L++ P P H LR D+ EE++K   +F K A+
Sbjct: 82  KGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAE 141

Query: 410 D 412
           +
Sbjct: 142 E 142
>sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 153

 Score =  123 bits (308), Expect = 3e-28
 Identities = 53/128 (41%), Positives = 82/128 (64%)
 Frame = +2

Query: 29  KAQITVFFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPI 208
           +A+++    I  +D S +  W GLL P   P+N+ AF + + F   +PF PP I  KT I
Sbjct: 16  EAKMSTLRNIESSDES-LLMWTGLLVPEKAPYNKGAFRIEINFPPQYPFMPPKILFKTKI 74

Query: 209 YHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNS 388
           YHPNVDE+G+VCL +I+ +NWKP  + EQ+++    ++  P+P H LR D+ EEF + + 
Sbjct: 75  YHPNVDEKGEVCLPIISTDNWKPTTRTEQVLQALVAIVHNPEPEHPLRSDLAEEFVREHK 134

Query: 389 EFMKKAKD 412
           +FMK A++
Sbjct: 135 KFMKTAEE 142
>sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
           (Ubiquitin carrier protein L6) (UbcH8) (Retinoic
           acid-induced gene B protein) (RIG-B)
          Length = 152

 Score =  102 bits (255), Expect = 4e-22
 Identities = 44/122 (36%), Positives = 72/122 (59%)
 Frame = +2

Query: 47  FFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVD 226
           + +   +D +N+  W  LL P  PP++  AF++ + F   +PFKPP I   T IYHPNVD
Sbjct: 20  YLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVD 79

Query: 227 EQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKA 406
           E G +CL +I++ NWKP  +  Q+++    L+++P+    LR D+ +  T+    F K A
Sbjct: 80  ENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNA 139

Query: 407 KD 412
           ++
Sbjct: 140 EE 141
>sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
           (Ubiquitin carrier protein L6) (UbcM8)
          Length = 152

 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 48/137 (35%), Positives = 76/137 (55%)
 Frame = +2

Query: 2   SKELAAVKDKAQITVFFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKP 181
           +KEL ++    ++  + +   +D +N+  W  LL P   P+   AF V + F   +PFKP
Sbjct: 7   AKELESLSK--ELPPYLRQLSSDDANVLVWHMLLLPDQLPYGLKAFQVRIDFpreYPFKP 64

Query: 182 PTITMKTPIYHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDV 361
           PT+   T IYHPNV E G VCL LI+  NWKP  +  Q+++    L+ KP+    +R ++
Sbjct: 65  PTLRFTTKIYHPNVREDGLVCLPLISNENWKPYTKPYQVLEALNVLVSKPNLEEPVRLEL 124

Query: 362 GEEFTKRNSEFMKKAKD 412
            +  T+    F KKA++
Sbjct: 125 ADLLTQNPEMFRKKAEE 141
>sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (hHR6B) (E2-17 kDa)
 sp|P63148|UBE2B_RABIT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E2(14k))
 sp|P63147|UBE2B_MOUSE Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E214K)
 sp|P63149|UBE2B_RAT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
           (Ubiquitin carrier protein B) (HR6B) (E2(14k))
          Length = 152

 Score = 79.3 bits (194), Expect = 5e-15
 Identities = 32/111 (28%), Positives = 60/111 (54%), Gaps = 1/111 (0%)
 Frame = +2

Query: 74  SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
           +NI +W  +++ P   PF +  F ++++FS+ +P KPPT+   + ++HPNV   G +CL 
Sbjct: 31  NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLD 90

Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 403
           ++  N W P      I+     L+D+P+PN        + + +   E+ K+
Sbjct: 91  ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubiquitin-protein ligase 1)
           (Ubiquitin carrier protein 1)
          Length = 192

 Score = 76.3 bits (186), Expect = 4e-14
 Identities = 33/112 (29%), Positives = 59/112 (52%), Gaps = 1/112 (0%)
 Frame = +2

Query: 77  NIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLAL 253
           NI  WE +++ P   PF +  F + L+F++ +P KPPT+   + ++HPNV   G +CL +
Sbjct: 32  NILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDI 91

Query: 254 INANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
           +  N W P      I+     L+D+P+PN        + + +   E+ K+ +
Sbjct: 92  LQ-NRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQ 142
>sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
           (Ubiquitin carrier protein A) (HR6A) (mHR6A)
 sp|P49459|UBE2A_HUMAN Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
           (Ubiquitin carrier protein A) (HR6A) (hHR6A)
          Length = 152

 Score = 75.9 bits (185), Expect = 6e-14
 Identities = 31/111 (27%), Positives = 58/111 (52%), Gaps = 1/111 (0%)
 Frame = +2

Query: 74  SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
           +NI  W  +++ P   PF +  F + ++F++ +P KPPT+   + ++HPNV   G +CL 
Sbjct: 31  NNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLD 90

Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 403
           ++  N W P      I+     L+D+P+PN        + + +   E+ K+
Sbjct: 91  ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 151

 Score = 73.9 bits (180), Expect = 2e-13
 Identities = 31/113 (27%), Positives = 58/113 (51%), Gaps = 1/113 (0%)
 Frame = +2

Query: 74  SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
           +NI  W  +++ P + PF +  F + ++F++ +P KPPT+   + ++HPNV   G +CL 
Sbjct: 31  NNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICLD 90

Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
           ++  N W P      I+     L+  P+PN        + + +   E+ K+ K
Sbjct: 91  ILQ-NRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYKENRREYEKRVK 142
>sp|Q9CQ37|UBE2T_MOUSE Ubiquitin-conjugating enzyme E2 T (Ubiquitin-protein ligase T)
           (Ubiquitin carrier protein T)
          Length = 204

 Score = 73.9 bits (180), Expect = 2e-13
 Identities = 33/107 (30%), Positives = 57/107 (53%), Gaps = 3/107 (2%)
 Frame = +2

Query: 98  LLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLALIN---ANN 268
           +L  +N P+ +  F + +   + +PF+PP +   TPIYHPN+D  G +CL ++       
Sbjct: 38  ILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLTPIYHPNIDSSGRICLDILKLPPKGA 97

Query: 269 WKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
           W+P +    ++     L+ +P+P+  L  D+  EF      F+KKAK
Sbjct: 98  WRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAK 144
>sp|P25865|UBC1_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 1 (Ubiquitin-protein ligase
           1) (Ubiquitin carrier protein 1)
          Length = 152

 Score = 72.8 bits (177), Expect = 5e-13
 Identities = 30/114 (26%), Positives = 62/114 (54%), Gaps = 1/114 (0%)
 Frame = +2

Query: 74  SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
           +NI  W  +++ P + P++   F + L+FS+++P KPPT+   + ++HPN+   G +CL 
Sbjct: 31  NNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLD 90

Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAKD 412
           ++  N W P+     I+     L+  P+PN     +    +++   E+ ++ +D
Sbjct: 91  ILQ-NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMYSESKREYNRRVRD 143
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,737,606
Number of Sequences: 369166
Number of extensions: 1087953
Number of successful extensions: 3050
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2942
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2996
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00186

  1. Dr_sW_005_K23
  2. Dr_sW_028_H05