Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_H05
(515 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (U... 133 3e-31
sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kD... 123 3e-28
sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (U... 102 4e-22
sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (U... 97 2e-20
sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ub... 80 2e-15
sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubi... 77 2e-14
sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ub... 77 2e-14
sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kD... 75 9e-14
sp|Q9CQ37|UBE2T_MOUSE Ubiquitin-conjugating enzyme E2 T (Ub... 75 9e-14
sp|P25865|UBC1_ARATH Ubiquitin-conjugating enzyme E2-17 kDa... 74 2e-13
>sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcM4)
sp|P68036|UB2L3_HUMAN Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcH7) (E2-F1) (L-UBC)
Length = 154
Score = 133 bits (334), Expect = 3e-31
Identities = 54/121 (44%), Positives = 82/121 (67%)
Frame = +2
Query: 41 FKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDE 220
F+ + D +N+ W+GL+ P NPP+++ AF + + F +PFKPP IT KT IYHPN+DE
Sbjct: 22 FRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDE 81
Query: 221 QGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 400
+G VCL +I+A NWKP + +Q+I+ L++ P P H LR D+ EE++K +F K A+
Sbjct: 82 KGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAE 141
Query: 401 D 403
+
Sbjct: 142 E 142
>sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 153
Score = 123 bits (308), Expect = 3e-28
Identities = 53/128 (41%), Positives = 82/128 (64%)
Frame = +2
Query: 20 KAEITVFFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPI 199
+A+++ I +D S + W GLL P P+N+ AF + + F +PF PP I KT I
Sbjct: 16 EAKMSTLRNIESSDES-LLMWTGLLVPEKAPYNKGAFRIEINFPPQYPFMPPKILFKTKI 74
Query: 200 YHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNS 379
YHPNVDE+G+VCL +I+ +NWKP + EQ+++ ++ P+P H LR D+ EEF + +
Sbjct: 75 YHPNVDEKGEVCLPIISTDNWKPTTRTEQVLQALVAIVHNPEPEHPLRSDLAEEFVREHK 134
Query: 380 EFMKKAKD 403
+FMK A++
Sbjct: 135 KFMKTAEE 142
>sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcH8) (Retinoic
acid-induced gene B protein) (RIG-B)
Length = 152
Score = 102 bits (255), Expect = 4e-22
Identities = 44/122 (36%), Positives = 72/122 (59%)
Frame = +2
Query: 38 FFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVD 217
+ + +D +N+ W LL P PP++ AF++ + F +PFKPP I T IYHPNVD
Sbjct: 20 YLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVD 79
Query: 218 EQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKA 397
E G +CL +I++ NWKP + Q+++ L+++P+ LR D+ + T+ F K A
Sbjct: 80 ENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNA 139
Query: 398 KD 403
++
Sbjct: 140 EE 141
>sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcM8)
Length = 152
Score = 97.1 bits (240), Expect = 2e-20
Identities = 46/126 (36%), Positives = 70/126 (55%)
Frame = +2
Query: 26 EITVFFKITKADPSNIFEWEGLLYPSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYH 205
E+ + + +D +N+ W LL P P+ AF V + F +PFKPPT+ T IYH
Sbjct: 16 ELPPYLRQLSSDDANVLVWHMLLLPDQLPYGLKAFQVRIDFpreYPFKPPTLRFTTKIYH 75
Query: 206 PNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEF 385
PNV E G VCL LI+ NWKP + Q+++ L+ KP+ +R ++ + T+ F
Sbjct: 76 PNVREDGLVCLPLISNENWKPYTKPYQVLEALNVLVSKPNLEEPVRLELADLLTQNPEMF 135
Query: 386 MKKAKD 403
KKA++
Sbjct: 136 RKKAEE 141
>sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (hHR6B) (E2-17 kDa)
sp|P63148|UBE2B_RABIT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E2(14k))
sp|P63147|UBE2B_MOUSE Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E214K)
sp|P63149|UBE2B_RAT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E2(14k))
Length = 152
Score = 80.5 bits (197), Expect = 2e-15
Identities = 33/111 (29%), Positives = 60/111 (54%), Gaps = 1/111 (0%)
Frame = +2
Query: 65 SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLA 241
+NI +W +++ P PF + F ++++FSE +P KPPT+ + ++HPNV G +CL
Sbjct: 31 NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLD 90
Query: 242 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 394
++ N W P I+ L+D+P+PN + + + E+ K+
Sbjct: 91 ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubiquitin-protein ligase 1)
(Ubiquitin carrier protein 1)
Length = 192
Score = 77.4 bits (189), Expect = 2e-14
Identities = 34/112 (30%), Positives = 59/112 (52%), Gaps = 1/112 (0%)
Frame = +2
Query: 68 NIFEWEGLLY-PSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLAL 244
NI WE +++ P PF + F + L+F+E +P KPPT+ + ++HPNV G +CL +
Sbjct: 32 NILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDI 91
Query: 245 INANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 400
+ N W P I+ L+D+P+PN + + + E+ K+ +
Sbjct: 92 LQ-NRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQ 142
>sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
(Ubiquitin carrier protein A) (HR6A) (mHR6A)
sp|P49459|UBE2A_HUMAN Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
(Ubiquitin carrier protein A) (HR6A) (hHR6A)
Length = 152
Score = 77.0 bits (188), Expect = 2e-14
Identities = 32/111 (28%), Positives = 58/111 (52%), Gaps = 1/111 (0%)
Frame = +2
Query: 65 SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLA 241
+NI W +++ P PF + F + ++F+E +P KPPT+ + ++HPNV G +CL
Sbjct: 31 NNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLD 90
Query: 242 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 394
++ N W P I+ L+D+P+PN + + + E+ K+
Sbjct: 91 ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 151
Score = 75.1 bits (183), Expect = 9e-14
Identities = 32/113 (28%), Positives = 58/113 (51%), Gaps = 1/113 (0%)
Frame = +2
Query: 65 SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLA 241
+NI W +++ P + PF + F + ++F+E +P KPPT+ + ++HPNV G +CL
Sbjct: 31 NNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICLD 90
Query: 242 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 400
++ N W P I+ L+ P+PN + + + E+ K+ K
Sbjct: 91 ILQ-NRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYKENRREYEKRVK 142
>sp|Q9CQ37|UBE2T_MOUSE Ubiquitin-conjugating enzyme E2 T (Ubiquitin-protein ligase T)
(Ubiquitin carrier protein T)
Length = 204
Score = 75.1 bits (183), Expect = 9e-14
Identities = 34/107 (31%), Positives = 57/107 (53%), Gaps = 3/107 (2%)
Frame = +2
Query: 89 LLYPSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLALIN---ANN 259
+L +N P+ + F + + E +PF+PP + TPIYHPN+D G +CL ++
Sbjct: 38 ILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLTPIYHPNIDSSGRICLDILKLPPKGA 97
Query: 260 WKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 400
W+P + ++ L+ +P+P+ L D+ EF F+KKAK
Sbjct: 98 WRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAK 144
>sp|P25865|UBC1_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 1 (Ubiquitin-protein ligase
1) (Ubiquitin carrier protein 1)
Length = 152
Score = 73.9 bits (180), Expect = 2e-13
Identities = 31/114 (27%), Positives = 62/114 (54%), Gaps = 1/114 (0%)
Frame = +2
Query: 65 SNIFEWEGLLY-PSNPPFNEFAFDVLLKFSENHPFKPPTITMKTPIYHPNVDEQGDVCLA 241
+NI W +++ P + P++ F + L+FSE++P KPPT+ + ++HPN+ G +CL
Sbjct: 31 NNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLD 90
Query: 242 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAKD 403
++ N W P+ I+ L+ P+PN + +++ E+ ++ +D
Sbjct: 91 ILQ-NQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMYSESKREYNRRVRD 143
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,344,690
Number of Sequences: 369166
Number of extensions: 1056917
Number of successful extensions: 3074
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3020
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)