Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_K23
(526 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (U... 130 2e-30
sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kD... 123 2e-28
sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (U... 100 2e-21
sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (U... 94 3e-19
sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ub... 77 3e-14
sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubi... 74 2e-13
sp|Q9CQ37|UBE2T_MOUSE Ubiquitin-conjugating enzyme E2 T (Ub... 74 2e-13
sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ub... 74 3e-13
sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kD... 72 6e-13
sp|P52490|UBC13_YEAST Ubiquitin-conjugating enzyme E2 13 (U... 72 6e-13
>sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcM4)
sp|P68036|UB2L3_HUMAN Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcH7) (E2-F1) (L-UBC)
Length = 154
Score = 130 bits (327), Expect = 2e-30
Identities = 53/121 (43%), Positives = 81/121 (66%)
Frame = +2
Query: 50 FKITKADPSTIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDE 229
F+ + D + + W+GL+ P NPP+++ AF + + F +PFKPP IT KT IYHPN+DE
Sbjct: 22 FRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDE 81
Query: 230 QGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
+G VCL +I+A NWKP + +Q+I+ L++ P P H LR D+ EE++K +F K A+
Sbjct: 82 KGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAE 141
Query: 410 D 412
+
Sbjct: 142 E 142
>sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 153
Score = 123 bits (309), Expect = 2e-28
Identities = 53/128 (41%), Positives = 82/128 (64%)
Frame = +2
Query: 29 KAQITVFFKITKADPSTIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPI 208
+A+++ I +D S + W GLL P P+N+ AF + + F +PF PP I KT I
Sbjct: 16 EAKMSTLRNIESSDESLLM-WTGLLVPEKAPYNKGAFRIEINFPPQYPFMPPKILFKTKI 74
Query: 209 YHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNS 388
YHPNVDE+G+VCL +I+ +NWKP + EQ+++ ++ P+P H LR D+ EEF + +
Sbjct: 75 YHPNVDEKGEVCLPIISTDNWKPTTRTEQVLQALVAIVHNPEPEHPLRSDLAEEFVREHK 134
Query: 389 EFMKKAKD 412
+FMK A++
Sbjct: 135 KFMKTAEE 142
>sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcH8) (Retinoic
acid-induced gene B protein) (RIG-B)
Length = 152
Score = 100 bits (249), Expect = 2e-21
Identities = 43/122 (35%), Positives = 71/122 (58%)
Frame = +2
Query: 47 FFKITKADPSTIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVD 226
+ + +D + + W LL P PP++ AF++ + F +PFKPP I T IYHPNVD
Sbjct: 20 YLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVD 79
Query: 227 EQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKA 406
E G +CL +I++ NWKP + Q+++ L+++P+ LR D+ + T+ F K A
Sbjct: 80 ENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNA 139
Query: 407 KD 412
++
Sbjct: 140 EE 141
>sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcM8)
Length = 152
Score = 93.6 bits (231), Expect = 3e-19
Identities = 47/137 (34%), Positives = 75/137 (54%)
Frame = +2
Query: 2 SKELAAVKDKAQITVFFKITKADPSTIFEWEGLLYPSNPPFNEFAFDVLLKFSDNHPFKP 181
+KEL ++ ++ + + +D + + W LL P P+ AF V + F +PFKP
Sbjct: 7 AKELESLSK--ELPPYLRQLSSDDANVLVWHMLLLPDQLPYGLKAFQVRIDFpreYPFKP 64
Query: 182 PTITMKTPIYHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDV 361
PT+ T IYHPNV E G VCL LI+ NWKP + Q+++ L+ KP+ +R ++
Sbjct: 65 PTLRFTTKIYHPNVREDGLVCLPLISNENWKPYTKPYQVLEALNVLVSKPNLEEPVRLEL 124
Query: 362 GEEFTKRNSEFMKKAKD 412
+ T+ F KKA++
Sbjct: 125 ADLLTQNPEMFRKKAEE 141
>sp|P63146|UBE2B_HUMAN Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (hHR6B) (E2-17 kDa)
sp|P63148|UBE2B_RABIT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E2(14k))
sp|P63147|UBE2B_MOUSE Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E214K)
sp|P63149|UBE2B_RAT Ubiquitin-conjugating enzyme E2 B (Ubiquitin-protein ligase B)
(Ubiquitin carrier protein B) (HR6B) (E2(14k))
Length = 152
Score = 77.0 bits (188), Expect = 3e-14
Identities = 31/111 (27%), Positives = 59/111 (53%), Gaps = 1/111 (0%)
Frame = +2
Query: 74 STIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
+ I +W +++ P PF + F ++++FS+ +P KPPT+ + ++HPNV G +CL
Sbjct: 31 NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLD 90
Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 403
++ N W P I+ L+D+P+PN + + + E+ K+
Sbjct: 91 ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P52478|UBC1_CAEEL Ubiquitin-conjugating enzyme E2 1 (Ubiquitin-protein ligase 1)
(Ubiquitin carrier protein 1)
Length = 192
Score = 73.9 bits (180), Expect = 2e-13
Identities = 32/111 (28%), Positives = 58/111 (52%), Gaps = 1/111 (0%)
Frame = +2
Query: 80 IFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLALI 256
I WE +++ P PF + F + L+F++ +P KPPT+ + ++HPNV G +CL ++
Sbjct: 33 ILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDIL 92
Query: 257 NANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
N W P I+ L+D+P+PN + + + E+ K+ +
Sbjct: 93 Q-NRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQ 142
>sp|Q9CQ37|UBE2T_MOUSE Ubiquitin-conjugating enzyme E2 T (Ubiquitin-protein ligase T)
(Ubiquitin carrier protein T)
Length = 204
Score = 73.9 bits (180), Expect = 2e-13
Identities = 33/107 (30%), Positives = 57/107 (53%), Gaps = 3/107 (2%)
Frame = +2
Query: 98 LLYPSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLALIN---ANN 268
+L +N P+ + F + + + +PF+PP + TPIYHPN+D G +CL ++
Sbjct: 38 ILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLTPIYHPNIDSSGRICLDILKLPPKGA 97
Query: 269 WKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAK 409
W+P + ++ L+ +P+P+ L D+ EF F+KKAK
Sbjct: 98 WRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAK 144
>sp|Q9Z255|UBE2A_MOUSE Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
(Ubiquitin carrier protein A) (HR6A) (mHR6A)
sp|P49459|UBE2A_HUMAN Ubiquitin-conjugating enzyme E2 A (Ubiquitin-protein ligase A)
(Ubiquitin carrier protein A) (HR6A) (hHR6A)
Length = 152
Score = 73.6 bits (179), Expect = 3e-13
Identities = 30/111 (27%), Positives = 57/111 (51%), Gaps = 1/111 (0%)
Frame = +2
Query: 74 STIFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLA 250
+ I W +++ P PF + F + ++F++ +P KPPT+ + ++HPNV G +CL
Sbjct: 31 NNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLD 90
Query: 251 LINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKK 403
++ N W P I+ L+D+P+PN + + + E+ K+
Sbjct: 91 ILQ-NRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR 140
>sp|P25153|UBCD6_DROME Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 151
Score = 72.4 bits (176), Expect = 6e-13
Identities = 35/131 (26%), Positives = 62/131 (47%), Gaps = 11/131 (8%)
Frame = +2
Query: 50 FKITKADPST----------IFEWEGLLY-PSNPPFNEFAFDVLLKFSDNHPFKPPTITM 196
FK + DP T I W +++ P + PF + F + ++F++ +P KPPT+
Sbjct: 13 FKRLQEDPPTGVSGAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRF 72
Query: 197 KTPIYHPNVDEQGDVCLALINANNWKPVVQAEQIIKEFAQLIDKPDPNHFLRGDVGEEFT 376
+ ++HPNV G +CL ++ N W P I+ L+ P+PN + +
Sbjct: 73 VSKVFHPNVYADGGICLDILQ-NRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYK 131
Query: 377 KRNSEFMKKAK 409
+ E+ K+ K
Sbjct: 132 ENRREYEKRVK 142
>sp|P52490|UBC13_YEAST Ubiquitin-conjugating enzyme E2 13 (Ubiquitin-protein ligase 13)
(Ubiquitin carrier protein 13)
Length = 153
Score = 72.4 bits (176), Expect = 6e-13
Identities = 32/102 (31%), Positives = 53/102 (51%)
Frame = +2
Query: 107 PSNPPFNEFAFDVLLKFSDNHPFKPPTITMKTPIYHPNVDEQGDVCLALINANNWKPVVQ 286
P P+ + F++ L D++P + P + T IYHPN+D G +CL ++ NW P +Q
Sbjct: 42 PEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLK-TNWSPALQ 100
Query: 287 AEQIIKEFAQLIDKPDPNHFLRGDVGEEFTKRNSEFMKKAKD 412
++ L+ P+PN L DV E++ K KA++
Sbjct: 101 IRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKARE 142
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,624,612
Number of Sequences: 369166
Number of extensions: 1085148
Number of successful extensions: 3037
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2927
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2984
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)