Planaria EST Database


DrC_00177

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00177
         (1033 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75689|CENA1_HUMAN  Centaurin-alpha 1 (Putative MAPK-acti...   153   9e-37
sp|Q9NPF8|CENA2_HUMAN  Centaurin-alpha 2                          130   5e-30
sp|Q9JK15|CENA2_RAT  Centaurin-alpha 2                            120   5e-27
sp|Q8R2V5|CENA2_MOUSE  Centaurin-alpha 2                          119   1e-26
sp|Q9ULH1|DDEF1_HUMAN  130-kDa phosphatidylinositol 4,5-biph...    66   1e-10
sp|Q9QWY8|DDEF1_MOUSE  130-kDa phosphatidylinositol 4,5-biph...    66   2e-10
sp|O97902|DDEF1_BOVIN  130-kDa phosphatidylinositol 4,5-biph...    65   2e-10
sp|O74345|UCP3_SCHPO  UBA-domain containing protein 3              60   1e-08
sp|Q8BZ05|CEND1_MOUSE  Centaurin-delta 1 (Cnt-d1)                  59   2e-08
sp|Q8WZ64|CEND1_HUMAN  Centaurin-delta 1 (Cnt-d1) (Arf-GAP, ...    59   3e-08
>sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-activating protein PM25)
          Length = 374

 Score =  153 bits (386), Expect = 9e-37
 Identities = 96/331 (29%), Positives = 162/331 (48%), Gaps = 5/331 (1%)
 Frame = +2

Query: 2   KLYNVPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSMENV-RVKDLNFDKISDLEI 178
           +L   PGN  CADC A  P +   +  + IC  C+G+HR++  V +VK +  D   + ++
Sbjct: 11  ELLQRPGNARCADCGAPDPDWASYTLGVFICLSCSGIHRNIPQVSKVKSVRLDAWEEAQV 70

Query: 179 EEVLKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITN 358
           E +   GN  +   +   +P FY  P  +  ++LR  +I AKY+ ++F   E   PY   
Sbjct: 71  EFMASHGNDAARARFESKVPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAG 130

Query: 359 QKLGLLKKRCKDKNIYNARFFRLSISDITFNYFIKETDASSYNRPQMKLDPKRNLRLDRI 538
            + G L KR +D   + +R F L+  +    YF         NR   K +PK  ++++ +
Sbjct: 131 YREGFLWKRGRDNGQFLSRKFVLTEREGALKYF---------NRNDAK-EPKAVMKIEHL 180

Query: 539 NATF----IENPDSRARIPDELLMISFFRQEEASWRHIYLKSNTPEDPVDWYQTIRFAKF 706
           NATF    I +P          L +++ +    S R+I++     ++ VDW+  +R A+F
Sbjct: 181 NATFQPAKIGHPHG--------LQVTYLKDN--STRNIFIYHEDGKEIVDWFNALRAARF 230

Query: 707 SLRAAYVVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSS 886
                   G    A++ +L   L+ +    G+M KTG + TE ++KRW  +  RRL+Y  
Sbjct: 231 HYLQVAFPG----ASDADLVPKLSRNYLKEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFK 286

Query: 887 GPLSPCGKGEIVIGERSRGYGAVLSSPPNAR 979
            PL    +GE+ IG +  GY  +   PP+ +
Sbjct: 287 DPLDAFARGEVFIGSKESGYTVLHGFPPSTQ 317
>sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2
          Length = 381

 Score =  130 bits (328), Expect = 5e-30
 Identities = 98/327 (29%), Positives = 153/327 (46%), Gaps = 7/327 (2%)
 Frame = +2

Query: 20  GNELCADCNAEKPGYVVLSYNILICRKCAGVHRSMENV-RVKDLNFDKISDLEIEEVLKL 196
           GN  CADC A  P +      I IC  C GVHR+  ++ RVK +  D   D  +E ++  
Sbjct: 21  GNAHCADCGAADPDWASYKLGIFICLNCCGVHRNFPDISRVKSVRLDFWDDSIVEFMIHN 80

Query: 197 GNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLL 376
           GN +    +   +P FY +P AN   +L+  +I AKY+ ++F + + +   +   + G L
Sbjct: 81  GNLRVKAKFEARVPAFYYIPQANDCLVLKEQWIRAKYERREF-MADGETISLPGNREGFL 139

Query: 377 KKRCKDKNIYNARFFRLSISDITFNYFIKETDASSYNRPQMKLDPKRNLRLDRINATF-- 550
            KR +D + +  R F L   +    YF KE   S          PK  + +  +NATF  
Sbjct: 140 WKRGRDNSQFLRRKFVLLAREGLLKYFTKEQGKS----------PKAVISIKDLNATFQT 189

Query: 551 --IENPDSRARIPDELLMISFFRQEEASWRHIYLKSNTPEDPVDWYQTIRFAKFSLRAAY 724
             I +P          L I++ R  +   R++++   + ++ VDW+  +R A    R  Y
Sbjct: 190 EKIGHPHG--------LQITYRR--DGHTRNLFVYHESGKEIVDWFNALRAA----RLQY 235

Query: 725 VVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLS 898
           +     E  E EL   LT +    G+M KTG +  E ++KRW  L    RRLLY   PL 
Sbjct: 236 LKMAFPELPESELVPFLTRNYLKQGFMEKTGPKQKEPFKKRWFALDCHERRLLYYKNPLD 295

Query: 899 PCGKGEIVIGERSRGYGAVLSSPPNAR 979
              +G++ +G + +GY A    P   R
Sbjct: 296 AFEQGQVFLGNKEQGYEAYEDLPKGIR 322
>sp|Q9JK15|CENA2_RAT Centaurin-alpha 2
          Length = 376

 Score =  120 bits (302), Expect = 5e-27
 Identities = 89/316 (28%), Positives = 149/316 (47%), Gaps = 7/316 (2%)
 Frame = +2

Query: 20  GNELCADCNAEKPGYVVLSYNILICRKCAGVHRSMENV-RVKDLNFDKISDLEIEEVLKL 196
           GN  CADC A  P +      + IC  C+GVHR+  ++ +VK +  D   D  +E +   
Sbjct: 21  GNGHCADCGAADPDWASYKLGVFICLHCSGVHRNFPDISKVKSVRLDFWDDSMVEFMTHN 80

Query: 197 GNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLL 376
           GN      +   +P FY +P A+   +L+  +I AKY+ ++F  ++   P     + G L
Sbjct: 81  GNLSVKAKFEARVPTFYYVPQASDCLVLKEQWIRAKYERQEFMAEKAVSP--PGDREGFL 138

Query: 377 KKRCKDKNIYNARFFRLSISDITFNYFIKETDASSYNRPQMKLDPKRNLRLDRINATF-- 550
            KR +D + +  R F L   +    Y+ KE   +          PK  + +  +NATF  
Sbjct: 139 WKRGRDNSQFLRRRFVLLSREGLLKYYTKEEGKT----------PKAIINIKDLNATFQT 188

Query: 551 --IENPDSRARIPDELLMISFFRQEEASWRHIYLKSNTPEDPVDWYQTIRFAKFSLRAAY 724
             I +P          L I++  ++E   R++++  ++ ++ VDW+  +R A    R  Y
Sbjct: 189 EKIGHPHG--------LQITY--RKEGQTRNLFVYHDSGKEIVDWFNALRAA----RLQY 234

Query: 725 VVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLS 898
           +     +  E EL   +T +    G+M KTG +  E ++KRW  L    RRLLY   PL 
Sbjct: 235 LKLAFPDLPESELVPLITRNYLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLD 294

Query: 899 PCGKGEIVIGERSRGY 946
              +G++ +G   +GY
Sbjct: 295 AFEQGQVFLGSNEQGY 310
>sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2
          Length = 381

 Score =  119 bits (299), Expect = 1e-26
 Identities = 93/318 (29%), Positives = 148/318 (46%), Gaps = 9/318 (2%)
 Frame = +2

Query: 20  GNELCADCNAEKPGYVVLSYNILICRKCAGVHRSMENV-RVKDLNFDKISDLEIEEVLKL 196
           GN  CADC A  P +      I IC  C+GVHR+  ++ +VK +  D   D  +E +   
Sbjct: 21  GNGHCADCGAADPDWASYKLGIFICLHCSGVHRNFPDISKVKSVRLDFWDDSMVEFMTHH 80

Query: 197 GNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSV--KEVDRPYITNQKLG 370
           GN      +   +P FY +P AN   +L+  +I AKY+ ++F+   K V  P     + G
Sbjct: 81  GNLNVKAKFEARVPAFYYVPQANDCLVLKEQWIRAKYERQEFTAIDKAVSHP---GNREG 137

Query: 371 LLKKRCKDKNIYNARFFRLSISDITFNYFIKETDASSYNRPQMKLDPKRNLRLDRINATF 550
            L KR +D   +  R F L   +    Y+ KE   +          PK  + +  +NATF
Sbjct: 138 FLWKRGRDNAQFLRRRFVLLSREGLLKYYTKEEGKA----------PKAVISIKDLNATF 187

Query: 551 ----IENPDSRARIPDELLMISFFRQEEASWRHIYLKSNTPEDPVDWYQTIRFAKFSLRA 718
               I +P          L I++  ++E   R++++  ++ ++ VDW+  +R A    R 
Sbjct: 188 QTEKIGHPHG--------LQITY--RKEGHTRNLFVYHDSGKEIVDWFNALRAA----RL 233

Query: 719 AYVVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGP 892
            Y+     +  E EL   +T +    G+M KTG +  E ++KRW  L    RRLLY   P
Sbjct: 234 QYLKLAFPDLPESELVPLITRNYLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNP 293

Query: 893 LSPCGKGEIVIGERSRGY 946
           L     G++ +G   +GY
Sbjct: 294 LDAFELGQVFLGSNEQGY 311
>sp|Q9ULH1|DDEF1_HUMAN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
           GTPase-activating protein (PIP2-dependent ARF1 GAP)
           (ADP-ribosylation factor-directed GTPase-activating
           protein 1) (ARF GTPase-activating protein 1)
           (Development and differentiation-enhancing factor 1)
          Length = 956

 Score = 66.2 bits (160), Expect = 1e-10
 Identities = 39/132 (29%), Positives = 66/132 (50%), Gaps = 2/132 (1%)
 Frame = +2

Query: 14  VPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSM--ENVRVKDLNFDKISDLEIEEV 187
           +PGN++C DC + +P ++  +  IL C +C+G+HR M     R++ L  DK+   E+   
Sbjct: 275 LPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLA 334

Query: 188 LKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKL 367
             +GN   ND     LP     P  +    +R+ +I AKY   +FS K       ++ KL
Sbjct: 335 KNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCS---TSSAKL 391

Query: 368 GLLKKRCKDKNI 403
             L +  K +++
Sbjct: 392 NELLEAIKSRDL 403
>sp|Q9QWY8|DDEF1_MOUSE 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
           GTPase-activating protein (PIP2-dependent ARF1 GAP)
           (ADP-ribosylation factor-directed GTPase-activating
           protein 1) (ARF GTPase-activating protein 1)
           (Development and differentiation-enhancing factor 1)
           (Differentiation-enhancing factor 1) (DEF-1)
          Length = 1147

 Score = 65.9 bits (159), Expect = 2e-10
 Identities = 35/108 (32%), Positives = 56/108 (51%), Gaps = 2/108 (1%)
 Frame = +2

Query: 14  VPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSM--ENVRVKDLNFDKISDLEIEEV 187
           +PGN++C DC + +P ++  +  IL C +C+G+HR M     R++ L  DK+   E+   
Sbjct: 463 LPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLA 522

Query: 188 LKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVK 331
             +GN   ND     LP     P  +    +R+ +I AKY   +FS K
Sbjct: 523 KNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRK 570
>sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
           GTPase-activating protein (PIP2-dependent ARF1 GAP)
           (ADP-ribosylation factor-directed GTPase-activating
           protein 1) (ARF GTPase-activating protein 1)
           (Development and differentiation-enhancing factor 1)
           (Differentiation-enhancing factor 1) (DEF-1)
          Length = 1129

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 39/132 (29%), Positives = 66/132 (50%), Gaps = 2/132 (1%)
 Frame = +2

Query: 14  VPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSM--ENVRVKDLNFDKISDLEIEEV 187
           +PGN++C DC + +P ++  +  IL C +C+G+HR M     R++ L  DK+   E+   
Sbjct: 451 LPGNDVCCDCGSAEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLA 510

Query: 188 LKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKL 367
             +GN   ND     LP     P  +    +R+ +I AKY   +FS K       ++ KL
Sbjct: 511 KNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSS---SSAKL 567

Query: 368 GLLKKRCKDKNI 403
             L +  K +++
Sbjct: 568 NELLEAIKSRDL 579
>sp|O74345|UCP3_SCHPO UBA-domain containing protein 3
          Length = 601

 Score = 59.7 bits (143), Expect = 1e-08
 Identities = 42/133 (31%), Positives = 67/133 (50%), Gaps = 3/133 (2%)
 Frame = +2

Query: 11  NVPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSMEN--VRVKDLNFDKISDLEIEE 184
           +V GN LCADC+     +   +  I +C +CA +HR +     +VK ++ D+ S+ +IE+
Sbjct: 16  SVSGNNLCADCSTRGVQWASWNLGIFLCLRCATIHRKLGTHVSKVKSISLDEWSNDQIEK 75

Query: 185 VLKLGNKKSNDGYLKFLPIFYRLP-NANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQ 361
           +   GN  +N  Y    P+ + LP NA   E +   +I  KY+ K F    +D  + TN 
Sbjct: 76  MKHWGNINAN-RYWNPNPLSHPLPTNALSDEHVMEKYIRDKYERKLF----LDENHSTNS 130

Query: 362 KLGLLKKRCKDKN 400
           K   L  R K  +
Sbjct: 131 KPPSLPPRTKSSS 143
>sp|Q8BZ05|CEND1_MOUSE Centaurin-delta 1 (Cnt-d1)
          Length = 1703

 Score = 58.9 bits (141), Expect = 2e-08
 Identities = 37/111 (33%), Positives = 61/111 (54%), Gaps = 4/111 (3%)
 Frame = +2

Query: 2    KLYNVPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSM--ENVRVKDLNFDKI--SD 169
            K++    N  CADC A  P +  ++  ++IC+KCAG HRS+  ++ +V+ L  D    S+
Sbjct: 688  KIWFNESNRSCADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDCKVRSLKMDASIWSN 747

Query: 170  LEIEEVLKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQF 322
              IE  + +GNK++ND +   L     L   + P   R++FI  KY+  +F
Sbjct: 748  ELIELFIVIGNKRANDFWAGNLQKDEEL-QVDSPVEKRKNFITQKYKEGKF 797
>sp|Q8WZ64|CEND1_HUMAN Centaurin-delta 1 (Cnt-d1) (Arf-GAP, Rho-GAP, ankyrin repeat and
            pleckstrin homology domain-containing protein 2) (PARX
            protein)
          Length = 1704

 Score = 58.5 bits (140), Expect = 3e-08
 Identities = 37/111 (33%), Positives = 62/111 (55%), Gaps = 4/111 (3%)
 Frame = +2

Query: 2    KLYNVPGNELCADCNAEKPGYVVLSYNILICRKCAGVHRSM--ENVRVKDLNFDKI--SD 169
            K++    N  CADC A  P +  ++  ++IC+KCAG HRS+  ++ +V+ L  D    S+
Sbjct: 690  KIWFNESNRSCADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDSKVRSLKMDASIWSN 749

Query: 170  LEIEEVLKLGNKKSNDGYLKFLPIFYRLPNANGPEILRRSFIIAKYQLKQF 322
              IE  + +GNK++ND +   L     L + + P   R++FI  KY+  +F
Sbjct: 750  ELIELFIVIGNKRANDFWAGNLQKDEEL-HMDSPVEKRKNFITQKYKEGKF 799
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 114,393,337
Number of Sequences: 369166
Number of extensions: 2287695
Number of successful extensions: 5320
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5085
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5298
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 11101059640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00177

  1. Dr_sW_005_H02
  2. Dr_sW_007_F12