Planaria EST Database

DrC_00118

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00118
         (611 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P07607|TYSY_MOUSE  Thymidylate synthase (TS) (TSase)           213   4e-55
sp|P04818|TYSY_HUMAN  Thymidylate synthase (TS) (TSase)           212   5e-55
sp|P45352|TYSY_RAT  Thymidylate synthase (TS) (TSase)             211   9e-55
sp|O76511|TYSY_DROME  Thymidylate synthase (TS) (TSase)           202   4e-52
sp|Q89940|TYSY_EHV2  Thymidylate synthase (TS) (TSase)            197   1e-50
sp|P09249|TYSY_VZVD  Thymidylate synthase (TS) (TSase)            193   3e-49
sp|P06854|TYSY_SHV21  Thymidylate synthase (TS) (TSase)           193   3e-49
sp|P12462|TYSY_HSVAT  Thymidylate synthase (TS) (TSase)           192   4e-49
sp|P90463|TYSY_HHV8  Thymidylate synthase (TS) (TSase)            191   1e-48
sp|P54081|TYSB_BACAM  Thymidylate synthase B (TS B) (TSase B)     178   1e-44

>sp|P07607|TYSY_MOUSE Thymidylate synthase (TS) (TSase)
          Length = 307

 Score =  213 bits (541), Expect = 4e-55
 Identities = 95/141 (67%), Positives = 118/141 (83%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           D++  ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T MIAH+TGL+PG F HTLGD+H+YLNHI+ ++ QL R+PR FPKLK  R+V+ I+DF 
Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFK 286

Query: 362 VDDFVIENYHPHPRIHMDMAL 424
           V+DF IE Y+PHP I M+MA+
Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307

>sp|P04818|TYSY_HUMAN Thymidylate synthase (TS) (TSase)
          Length = 313

 Score =  212 bits (540), Expect = 5e-55
 Identities = 93/141 (65%), Positives = 118/141 (83%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           D++  ++ AWNP+DLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 173 DDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYAL 232

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T MIAH+TGLKPG F HTLGD+H+YLNHI+ ++ QL R+PR FPKL+  R+V++I+DF 
Sbjct: 233 LTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFK 292

Query: 362 VDDFVIENYHPHPRIHMDMAL 424
            +DF IE Y+PHP I M+MA+
Sbjct: 293 AEDFQIEGYNPHPTIKMEMAV 313

>sp|P45352|TYSY_RAT Thymidylate synthase (TS) (TSase)
          Length = 307

 Score =  211 bits (538), Expect = 9e-55
 Identities = 94/141 (66%), Positives = 118/141 (83%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           D++  ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T MIAH+TGL+PG F HTLGD+H+YLNHI+ ++ QL R+PR FPKL+  R+V+ I+DF 
Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFK 286

Query: 362 VDDFVIENYHPHPRIHMDMAL 424
           V+DF IE Y+PHP I M+MA+
Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307

>sp|O76511|TYSY_DROME Thymidylate synthase (TS) (TSase)
          Length = 321

 Score =  202 bits (515), Expect = 4e-52
 Identities = 92/138 (66%), Positives = 112/138 (81%), Gaps = 2/138 (1%)
 Frame = +2

Query: 17  VVMAWNPKDLPLMALPPCHCLVQFFVEDR--ELSCKLYQRSGDMGLGVPFNIASYAIFTC 190
           ++ AWNP D+P MALPPCHCL QF+V ++  ELSC+LYQRS DMGLGVPFNIASYA+ T 
Sbjct: 184 IMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTH 243

Query: 191 MIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDD 370
           MIAHVTGLKPG F HT+GD+HVYLNH++ ++ QL R PR FPKL  +RQV  IEDF  +D
Sbjct: 244 MIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFED 303

Query: 371 FVIENYHPHPRIHMDMAL 424
           F I +Y+PHP+I MDMA+
Sbjct: 304 FQIVDYNPHPKIQMDMAV 321

>sp|Q89940|TYSY_EHV2 Thymidylate synthase (TS) (TSase)
          Length = 289

 Score =  197 bits (502), Expect = 1e-50
 Identities = 86/141 (60%), Positives = 112/141 (79%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           +++  V+ AWNP DLP MALPPCH L QF+V   ELSC+LYQRSGDMGLGVPFNIASY++
Sbjct: 149 ESRRLVLTAWNPADLPAMALPPCHLLCQFYVAGGELSCQLYQRSGDMGLGVPFNIASYSL 208

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T M+AH+TGL+PG F H LGD+HVYLNH++ ++ QL+R PR FP+L+  R+V+ I+DF 
Sbjct: 209 LTYMVAHLTGLEPGDFIHVLGDAHVYLNHVEPLKLQLTRSPRPFPRLRILRRVEDIDDFR 268

Query: 362 VDDFVIENYHPHPRIHMDMAL 424
            +DF +E YHPH  I M+MA+
Sbjct: 269 AEDFALEGYHPHAAIPMEMAV 289

>sp|P09249|TYSY_VZVD Thymidylate synthase (TS) (TSase)
          Length = 301

 Score =  193 bits (491), Expect = 3e-49
 Identities = 84/141 (59%), Positives = 110/141 (78%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           +++  ++ +WNPKD+PLM LPPCH L QF+V + ELSC++YQRSGDMGLGVPFNIA YA+
Sbjct: 161 ESRRMIISSWNPKDIPLMVLPPCHTLCQFYVANGELSCQVYQRSGDMGLGVPFNIAGYAL 220

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T ++AHVTGLK G   HT+GD+H+YLNHIDA++ QL+R P+ FP LK  R V  I DF 
Sbjct: 221 LTYIVAHVTGLKTGDLIHTMGDAHIYLNHIDALKVQLARSPKPFPCLKIIRNVTDINDFK 280

Query: 362 VDDFVIENYHPHPRIHMDMAL 424
            DDF ++ Y+PHP + M+MAL
Sbjct: 281 WDDFQLDGYNPHPPLKMEMAL 301

>sp|P06854|TYSY_SHV21 Thymidylate synthase (TS) (TSase)
          Length = 294

 Score =  193 bits (490), Expect = 3e-49
 Identities = 85/140 (60%), Positives = 109/140 (77%)
 Frame = +2

Query: 5   NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184
           ++  ++ AWN  D+P M LPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++ 
Sbjct: 155 DRRMLMCAWNVSDIPKMVLPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 214

Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364
           TCMIAHVT L PG F HT+GD+H+Y++HIDA++ QL+R PR FP L+F R V  I+DF  
Sbjct: 215 TCMIAHVTNLVPGEFIHTIGDAHIYVDHIDALKMQLTRTPRPFPTLRFARNVSCIDDFKA 274

Query: 365 DDFVIENYHPHPRIHMDMAL 424
           DD ++ENY+PHP I M MA+
Sbjct: 275 DDIILENYNPHPIIKMHMAV 294

>sp|P12462|TYSY_HSVAT Thymidylate synthase (TS) (TSase)
          Length = 290

 Score =  192 bits (489), Expect = 4e-49
 Identities = 87/140 (62%), Positives = 110/140 (78%)
 Frame = +2

Query: 5   NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184
           ++  ++ AWN  D+P MALPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++ 
Sbjct: 151 DRRMLMCAWNVLDVPKMALPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 210

Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364
           TCMIAHVT L PG F HTLGD+HVY+NH+DA+  QL+R PR FP LKF R+V  I+DF  
Sbjct: 211 TCMIAHVTDLVPGEFIHTLGDAHVYVNHVDALTEQLTRTPRPFPTLKFARKVASIDDFKA 270

Query: 365 DDFVIENYHPHPRIHMDMAL 424
           +D ++ENY+P+P I M MA+
Sbjct: 271 NDIILENYNPYPSIKMPMAV 290

>sp|P90463|TYSY_HHV8 Thymidylate synthase (TS) (TSase)
          Length = 337

 Score =  191 bits (486), Expect = 1e-48
 Identities = 86/136 (63%), Positives = 107/136 (78%)
 Frame = +2

Query: 17  VVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIFTCMI 196
           ++ AWNP DL LMALPPCH L QF+V D ELSC+LYQRSGDMGLGVPFNIASY++ T M+
Sbjct: 202 IMCAWNPADLSLMALPPCHLLCQFYVADGELSCQLYQRSGDMGLGVPFNIASYSLLTYML 261

Query: 197 AHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDDFV 376
           AHVTGL+PG F HTLGD+H+Y  HI+ +R QL+R PR FP+L+  R V  +E+F  DDF 
Sbjct: 262 AHVTGLRPGEFIHTLGDAHIYKTHIEPLRLQLTRTPRPFPRLEILRSVSSMEEFTPDDFR 321

Query: 377 IENYHPHPRIHMDMAL 424
           + +Y PHP I M+MA+
Sbjct: 322 LVDYCPHPTIRMEMAV 337

>sp|P54081|TYSB_BACAM Thymidylate synthase B (TS B) (TSase B)
          Length = 264

 Score =  178 bits (451), Expect = 1e-44
 Identities = 80/135 (59%), Positives = 101/135 (74%)
 Frame = +2

Query: 2   DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
           +++  +V AWN  ++  MALPPCHCL QF+V D +LSC+LYQRS D+ LGVPFNIASYA+
Sbjct: 124 NSRRLIVSAWNVGEIDRMALPPCHCLFQFYVADGKLSCQLYQRSADVFLGVPFNIASYAL 183

Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHIDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
            T MIAHVTGL+PG F HT GD H+Y NH++ +  QL+R  R  PKL+F R VD I DF 
Sbjct: 184 LTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIFDFA 243

Query: 362 VDDFVIENYHPHPRI 406
            +DF+IE+Y PHP I
Sbjct: 244 FEDFIIEDYDPHPHI 258

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,088,102
Number of Sequences: 369166
Number of extensions: 1617277
Number of successful extensions: 4936
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4669
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4870
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4797864890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00118

  1. Dr_sW_003_P13
  2. Dr_sW_023_B21