Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_003_P13 (554 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P07607|TYSY_MOUSE Thymidylate synthase (TS) (TSase) 212 4e-55 sp|P04818|TYSY_HUMAN Thymidylate synthase (TS) (TSase) 212 6e-55 sp|P45352|TYSY_RAT Thymidylate synthase (TS) (TSase) 211 1e-54 sp|O76511|TYSY_DROME Thymidylate synthase (TS) (TSase) 203 3e-52 sp|Q89940|TYSY_EHV2 Thymidylate synthase (TS) (TSase) 198 8e-51 sp|P09249|TYSY_VZVD Thymidylate synthase (TS) (TSase) 193 3e-49 sp|P12462|TYSY_HSVAT Thymidylate synthase (TS) (TSase) 193 3e-49 sp|P06854|TYSY_SHV21 Thymidylate synthase (TS) (TSase) 192 4e-49 sp|P90463|TYSY_HHV8 Thymidylate synthase (TS) (TSase) 191 1e-48 sp|P54081|TYSB_BACAM Thymidylate synthase B (TS B) (TSase B) 178 7e-45
>sp|P07607|TYSY_MOUSE Thymidylate synthase (TS) (TSase) Length = 307 Score = 212 bits (540), Expect = 4e-55 Identities = 94/141 (66%), Positives = 118/141 (83%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 D++ ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+ Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T MIAH+TGL+PG F HTLGD+H+YLNH++ ++ QL R+PR FPKLK R+V+ I+DF Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFK 286 Query: 362 VDDFVIENYHPHPRIHMDMAL 424 V+DF IE Y+PHP I M+MA+ Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307
>sp|P04818|TYSY_HUMAN Thymidylate synthase (TS) (TSase) Length = 313 Score = 212 bits (539), Expect = 6e-55 Identities = 92/141 (65%), Positives = 118/141 (83%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 D++ ++ AWNP+DLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+ Sbjct: 173 DDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYAL 232 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T MIAH+TGLKPG F HTLGD+H+YLNH++ ++ QL R+PR FPKL+ R+V++I+DF Sbjct: 233 LTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFK 292 Query: 362 VDDFVIENYHPHPRIHMDMAL 424 +DF IE Y+PHP I M+MA+ Sbjct: 293 AEDFQIEGYNPHPTIKMEMAV 313
>sp|P45352|TYSY_RAT Thymidylate synthase (TS) (TSase) Length = 307 Score = 211 bits (537), Expect = 1e-54 Identities = 93/141 (65%), Positives = 118/141 (83%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 D++ ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+ Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T MIAH+TGL+PG F HTLGD+H+YLNH++ ++ QL R+PR FPKL+ R+V+ I+DF Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFK 286 Query: 362 VDDFVIENYHPHPRIHMDMAL 424 V+DF IE Y+PHP I M+MA+ Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307
>sp|O76511|TYSY_DROME Thymidylate synthase (TS) (TSase) Length = 321 Score = 203 bits (516), Expect = 3e-52 Identities = 93/138 (67%), Positives = 112/138 (81%), Gaps = 2/138 (1%) Frame = +2 Query: 17 VVMAWNPKDLPLMALPPCHCLVQFFVEDR--ELSCKLYQRSGDMGLGVPFNIASYAIFTC 190 ++ AWNP D+P MALPPCHCL QF+V ++ ELSC+LYQRS DMGLGVPFNIASYA+ T Sbjct: 184 IMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTH 243 Query: 191 MIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDD 370 MIAHVTGLKPG F HT+GD+HVYLNHV+ ++ QL R PR FPKL +RQV IEDF +D Sbjct: 244 MIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFED 303 Query: 371 FVIENYHPHPRIHMDMAL 424 F I +Y+PHP+I MDMA+ Sbjct: 304 FQIVDYNPHPKIQMDMAV 321
>sp|Q89940|TYSY_EHV2 Thymidylate synthase (TS) (TSase) Length = 289 Score = 198 bits (503), Expect = 8e-51 Identities = 87/141 (61%), Positives = 112/141 (79%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 +++ V+ AWNP DLP MALPPCH L QF+V ELSC+LYQRSGDMGLGVPFNIASY++ Sbjct: 149 ESRRLVLTAWNPADLPAMALPPCHLLCQFYVAGGELSCQLYQRSGDMGLGVPFNIASYSL 208 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T M+AH+TGL+PG F H LGD+HVYLNHV+ ++ QL+R PR FP+L+ R+V+ I+DF Sbjct: 209 LTYMVAHLTGLEPGDFIHVLGDAHVYLNHVEPLKLQLTRSPRPFPRLRILRRVEDIDDFR 268 Query: 362 VDDFVIENYHPHPRIHMDMAL 424 +DF +E YHPH I M+MA+ Sbjct: 269 AEDFALEGYHPHAAIPMEMAV 289
>sp|P09249|TYSY_VZVD Thymidylate synthase (TS) (TSase) Length = 301 Score = 193 bits (490), Expect = 3e-49 Identities = 83/141 (58%), Positives = 110/141 (78%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 +++ ++ +WNPKD+PLM LPPCH L QF+V + ELSC++YQRSGDMGLGVPFNIA YA+ Sbjct: 161 ESRRMIISSWNPKDIPLMVLPPCHTLCQFYVANGELSCQVYQRSGDMGLGVPFNIAGYAL 220 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T ++AHVTGLK G HT+GD+H+YLNH+DA++ QL+R P+ FP LK R V I DF Sbjct: 221 LTYIVAHVTGLKTGDLIHTMGDAHIYLNHIDALKVQLARSPKPFPCLKIIRNVTDINDFK 280 Query: 362 VDDFVIENYHPHPRIHMDMAL 424 DDF ++ Y+PHP + M+MAL Sbjct: 281 WDDFQLDGYNPHPPLKMEMAL 301
>sp|P12462|TYSY_HSVAT Thymidylate synthase (TS) (TSase) Length = 290 Score = 193 bits (490), Expect = 3e-49 Identities = 88/140 (62%), Positives = 110/140 (78%) Frame = +2 Query: 5 NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184 ++ ++ AWN D+P MALPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++ Sbjct: 151 DRRMLMCAWNVLDVPKMALPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 210 Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364 TCMIAHVT L PG F HTLGD+HVY+NHVDA+ QL+R PR FP LKF R+V I+DF Sbjct: 211 TCMIAHVTDLVPGEFIHTLGDAHVYVNHVDALTEQLTRTPRPFPTLKFARKVASIDDFKA 270 Query: 365 DDFVIENYHPHPRIHMDMAL 424 +D ++ENY+P+P I M MA+ Sbjct: 271 NDIILENYNPYPSIKMPMAV 290
>sp|P06854|TYSY_SHV21 Thymidylate synthase (TS) (TSase) Length = 294 Score = 192 bits (489), Expect = 4e-49 Identities = 84/140 (60%), Positives = 109/140 (77%) Frame = +2 Query: 5 NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184 ++ ++ AWN D+P M LPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++ Sbjct: 155 DRRMLMCAWNVSDIPKMVLPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 214 Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364 TCMIAHVT L PG F HT+GD+H+Y++H+DA++ QL+R PR FP L+F R V I+DF Sbjct: 215 TCMIAHVTNLVPGEFIHTIGDAHIYVDHIDALKMQLTRTPRPFPTLRFARNVSCIDDFKA 274 Query: 365 DDFVIENYHPHPRIHMDMAL 424 DD ++ENY+PHP I M MA+ Sbjct: 275 DDIILENYNPHPIIKMHMAV 294
>sp|P90463|TYSY_HHV8 Thymidylate synthase (TS) (TSase) Length = 337 Score = 191 bits (485), Expect = 1e-48 Identities = 85/136 (62%), Positives = 107/136 (78%) Frame = +2 Query: 17 VVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIFTCMI 196 ++ AWNP DL LMALPPCH L QF+V D ELSC+LYQRSGDMGLGVPFNIASY++ T M+ Sbjct: 202 IMCAWNPADLSLMALPPCHLLCQFYVADGELSCQLYQRSGDMGLGVPFNIASYSLLTYML 261 Query: 197 AHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDDFV 376 AHVTGL+PG F HTLGD+H+Y H++ +R QL+R PR FP+L+ R V +E+F DDF Sbjct: 262 AHVTGLRPGEFIHTLGDAHIYKTHIEPLRLQLTRTPRPFPRLEILRSVSSMEEFTPDDFR 321 Query: 377 IENYHPHPRIHMDMAL 424 + +Y PHP I M+MA+ Sbjct: 322 LVDYCPHPTIRMEMAV 337
>sp|P54081|TYSB_BACAM Thymidylate synthase B (TS B) (TSase B) Length = 264 Score = 178 bits (452), Expect = 7e-45 Identities = 81/135 (60%), Positives = 101/135 (74%) Frame = +2 Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181 +++ +V AWN ++ MALPPCHCL QF+V D +LSC+LYQRS D+ LGVPFNIASYA+ Sbjct: 124 NSRRLIVSAWNVGEIDRMALPPCHCLFQFYVADGKLSCQLYQRSADVFLGVPFNIASYAL 183 Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361 T MIAHVTGL+PG F HT GD H+Y NHV+ + QL+R R PKL+F R VD I DF Sbjct: 184 LTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIFDFA 243 Query: 362 VDDFVIENYHPHPRI 406 +DF+IE+Y PHP I Sbjct: 244 FEDFIIEDYDPHPHI 258
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 68,926,763 Number of Sequences: 369166 Number of extensions: 1485894 Number of successful extensions: 4521 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4259 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4455 length of database: 68,354,980 effective HSP length: 104 effective length of database: 49,142,540 effective search space used: 3931403200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)