Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_P13
(554 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07607|TYSY_MOUSE Thymidylate synthase (TS) (TSase) 212 4e-55
sp|P04818|TYSY_HUMAN Thymidylate synthase (TS) (TSase) 212 6e-55
sp|P45352|TYSY_RAT Thymidylate synthase (TS) (TSase) 211 1e-54
sp|O76511|TYSY_DROME Thymidylate synthase (TS) (TSase) 203 3e-52
sp|Q89940|TYSY_EHV2 Thymidylate synthase (TS) (TSase) 198 8e-51
sp|P09249|TYSY_VZVD Thymidylate synthase (TS) (TSase) 193 3e-49
sp|P12462|TYSY_HSVAT Thymidylate synthase (TS) (TSase) 193 3e-49
sp|P06854|TYSY_SHV21 Thymidylate synthase (TS) (TSase) 192 4e-49
sp|P90463|TYSY_HHV8 Thymidylate synthase (TS) (TSase) 191 1e-48
sp|P54081|TYSB_BACAM Thymidylate synthase B (TS B) (TSase B) 178 7e-45
>sp|P07607|TYSY_MOUSE Thymidylate synthase (TS) (TSase)
Length = 307
Score = 212 bits (540), Expect = 4e-55
Identities = 94/141 (66%), Positives = 118/141 (83%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
D++ ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T MIAH+TGL+PG F HTLGD+H+YLNH++ ++ QL R+PR FPKLK R+V+ I+DF
Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFK 286
Query: 362 VDDFVIENYHPHPRIHMDMAL 424
V+DF IE Y+PHP I M+MA+
Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307
>sp|P04818|TYSY_HUMAN Thymidylate synthase (TS) (TSase)
Length = 313
Score = 212 bits (539), Expect = 6e-55
Identities = 92/141 (65%), Positives = 118/141 (83%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
D++ ++ AWNP+DLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 173 DDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYAL 232
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T MIAH+TGLKPG F HTLGD+H+YLNH++ ++ QL R+PR FPKL+ R+V++I+DF
Sbjct: 233 LTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFK 292
Query: 362 VDDFVIENYHPHPRIHMDMAL 424
+DF IE Y+PHP I M+MA+
Sbjct: 293 AEDFQIEGYNPHPTIKMEMAV 313
>sp|P45352|TYSY_RAT Thymidylate synthase (TS) (TSase)
Length = 307
Score = 211 bits (537), Expect = 1e-54
Identities = 93/141 (65%), Positives = 118/141 (83%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
D++ ++ AWNPKDLPLMALPPCH L QF+V + ELSC+LYQRSGDMGLGVPFNIASYA+
Sbjct: 167 DDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYAL 226
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T MIAH+TGL+PG F HTLGD+H+YLNH++ ++ QL R+PR FPKL+ R+V+ I+DF
Sbjct: 227 LTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFK 286
Query: 362 VDDFVIENYHPHPRIHMDMAL 424
V+DF IE Y+PHP I M+MA+
Sbjct: 287 VEDFQIEGYNPHPTIKMEMAV 307
>sp|O76511|TYSY_DROME Thymidylate synthase (TS) (TSase)
Length = 321
Score = 203 bits (516), Expect = 3e-52
Identities = 93/138 (67%), Positives = 112/138 (81%), Gaps = 2/138 (1%)
Frame = +2
Query: 17 VVMAWNPKDLPLMALPPCHCLVQFFVEDR--ELSCKLYQRSGDMGLGVPFNIASYAIFTC 190
++ AWNP D+P MALPPCHCL QF+V ++ ELSC+LYQRS DMGLGVPFNIASYA+ T
Sbjct: 184 IMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTH 243
Query: 191 MIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDD 370
MIAHVTGLKPG F HT+GD+HVYLNHV+ ++ QL R PR FPKL +RQV IEDF +D
Sbjct: 244 MIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFED 303
Query: 371 FVIENYHPHPRIHMDMAL 424
F I +Y+PHP+I MDMA+
Sbjct: 304 FQIVDYNPHPKIQMDMAV 321
>sp|Q89940|TYSY_EHV2 Thymidylate synthase (TS) (TSase)
Length = 289
Score = 198 bits (503), Expect = 8e-51
Identities = 87/141 (61%), Positives = 112/141 (79%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
+++ V+ AWNP DLP MALPPCH L QF+V ELSC+LYQRSGDMGLGVPFNIASY++
Sbjct: 149 ESRRLVLTAWNPADLPAMALPPCHLLCQFYVAGGELSCQLYQRSGDMGLGVPFNIASYSL 208
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T M+AH+TGL+PG F H LGD+HVYLNHV+ ++ QL+R PR FP+L+ R+V+ I+DF
Sbjct: 209 LTYMVAHLTGLEPGDFIHVLGDAHVYLNHVEPLKLQLTRSPRPFPRLRILRRVEDIDDFR 268
Query: 362 VDDFVIENYHPHPRIHMDMAL 424
+DF +E YHPH I M+MA+
Sbjct: 269 AEDFALEGYHPHAAIPMEMAV 289
>sp|P09249|TYSY_VZVD Thymidylate synthase (TS) (TSase)
Length = 301
Score = 193 bits (490), Expect = 3e-49
Identities = 83/141 (58%), Positives = 110/141 (78%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
+++ ++ +WNPKD+PLM LPPCH L QF+V + ELSC++YQRSGDMGLGVPFNIA YA+
Sbjct: 161 ESRRMIISSWNPKDIPLMVLPPCHTLCQFYVANGELSCQVYQRSGDMGLGVPFNIAGYAL 220
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T ++AHVTGLK G HT+GD+H+YLNH+DA++ QL+R P+ FP LK R V I DF
Sbjct: 221 LTYIVAHVTGLKTGDLIHTMGDAHIYLNHIDALKVQLARSPKPFPCLKIIRNVTDINDFK 280
Query: 362 VDDFVIENYHPHPRIHMDMAL 424
DDF ++ Y+PHP + M+MAL
Sbjct: 281 WDDFQLDGYNPHPPLKMEMAL 301
>sp|P12462|TYSY_HSVAT Thymidylate synthase (TS) (TSase)
Length = 290
Score = 193 bits (490), Expect = 3e-49
Identities = 88/140 (62%), Positives = 110/140 (78%)
Frame = +2
Query: 5 NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184
++ ++ AWN D+P MALPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++
Sbjct: 151 DRRMLMCAWNVLDVPKMALPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 210
Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364
TCMIAHVT L PG F HTLGD+HVY+NHVDA+ QL+R PR FP LKF R+V I+DF
Sbjct: 211 TCMIAHVTDLVPGEFIHTLGDAHVYVNHVDALTEQLTRTPRPFPTLKFARKVASIDDFKA 270
Query: 365 DDFVIENYHPHPRIHMDMAL 424
+D ++ENY+P+P I M MA+
Sbjct: 271 NDIILENYNPYPSIKMPMAV 290
>sp|P06854|TYSY_SHV21 Thymidylate synthase (TS) (TSase)
Length = 294
Score = 192 bits (489), Expect = 4e-49
Identities = 84/140 (60%), Positives = 109/140 (77%)
Frame = +2
Query: 5 NKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIF 184
++ ++ AWN D+P M LPPCH L QF+V D +LSC+LYQRS DMGLGVPFNIASY++
Sbjct: 155 DRRMLMCAWNVSDIPKMVLPPCHVLSQFYVCDGKLSCQLYQRSADMGLGVPFNIASYSLL 214
Query: 185 TCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHV 364
TCMIAHVT L PG F HT+GD+H+Y++H+DA++ QL+R PR FP L+F R V I+DF
Sbjct: 215 TCMIAHVTNLVPGEFIHTIGDAHIYVDHIDALKMQLTRTPRPFPTLRFARNVSCIDDFKA 274
Query: 365 DDFVIENYHPHPRIHMDMAL 424
DD ++ENY+PHP I M MA+
Sbjct: 275 DDIILENYNPHPIIKMHMAV 294
>sp|P90463|TYSY_HHV8 Thymidylate synthase (TS) (TSase)
Length = 337
Score = 191 bits (485), Expect = 1e-48
Identities = 85/136 (62%), Positives = 107/136 (78%)
Frame = +2
Query: 17 VVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAIFTCMI 196
++ AWNP DL LMALPPCH L QF+V D ELSC+LYQRSGDMGLGVPFNIASY++ T M+
Sbjct: 202 IMCAWNPADLSLMALPPCHLLCQFYVADGELSCQLYQRSGDMGLGVPFNIASYSLLTYML 261
Query: 197 AHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFHVDDFV 376
AHVTGL+PG F HTLGD+H+Y H++ +R QL+R PR FP+L+ R V +E+F DDF
Sbjct: 262 AHVTGLRPGEFIHTLGDAHIYKTHIEPLRLQLTRTPRPFPRLEILRSVSSMEEFTPDDFR 321
Query: 377 IENYHPHPRIHMDMAL 424
+ +Y PHP I M+MA+
Sbjct: 322 LVDYCPHPTIRMEMAV 337
>sp|P54081|TYSB_BACAM Thymidylate synthase B (TS B) (TSase B)
Length = 264
Score = 178 bits (452), Expect = 7e-45
Identities = 81/135 (60%), Positives = 101/135 (74%)
Frame = +2
Query: 2 DNKNHVVMAWNPKDLPLMALPPCHCLVQFFVEDRELSCKLYQRSGDMGLGVPFNIASYAI 181
+++ +V AWN ++ MALPPCHCL QF+V D +LSC+LYQRS D+ LGVPFNIASYA+
Sbjct: 124 NSRRLIVSAWNVGEIDRMALPPCHCLFQFYVADGKLSCQLYQRSADVFLGVPFNIASYAL 183
Query: 182 FTCMIAHVTGLKPGRFFHTLGDSHVYLNHVDAMRTQLSRQPRDFPKLKFRRQVDRIEDFH 361
T MIAHVTGL+PG F HT GD H+Y NHV+ + QL+R R PKL+F R VD I DF
Sbjct: 184 LTMMIAHVTGLEPGEFVHTFGDVHIYQNHVEQVNLQLTRDVRPLPKLRFARNVDSIFDFA 243
Query: 362 VDDFVIENYHPHPRI 406
+DF+IE+Y PHP I
Sbjct: 244 FEDFIIEDYDPHPHI 258
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,926,763
Number of Sequences: 369166
Number of extensions: 1485894
Number of successful extensions: 4521
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4455
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3931403200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)