Planaria EST Database

DrC_00110

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00110
         (956 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P00491|PNPH_HUMAN  Purine nucleoside phosphorylase (Inosi...   250   5e-66
sp|P23492|PNPH_MOUSE  Purine nucleoside phosphorylase (Inosi...   248   2e-65
sp|P55859|PNPH_BOVIN  Purine nucleoside phosphorylase (Inosi...   243   4e-64
sp|P77834|PUNA_BACST  Purine nucleoside phosphorylase I (PNP...   213   8e-55
sp|Q05788|PNPH_YEAST  Purine nucleoside phosphorylase (Inosi...   202   1e-51
sp|P46354|PUNA_BACSU  Purine nucleoside phosphorylase I (PNP...   196   8e-50
sp|P45563|XAPA_ECOLI  Xanthosine phosphorylase                    167   4e-41
sp|P46862|PUNA_MYCLE  Purine nucleoside phosphorylase (Inosi...   142   1e-33
sp|P0A539|PUNA_MYCBO  Purine nucleoside phosphorylase (Inosi...   139   2e-32
sp|P81989|PUNA_CELSP  Purine nucleoside phosphorylase (Inosi...   103   9e-22

>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  250 bits (638), Expect = 5e-66
 Identities = 122/269 (45%), Positives = 175/269 (65%)
 Frame = +1

Query: 7   AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
           AE++ S    +P+             + +T   I  Y+EI  FP+STV GH G LVFG L
Sbjct: 14  AEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFL 73

Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
            G+  V+MQGRFH YEGY       PVRV HL+GV  L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74  NGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIR 133

Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
           DHIN PG  G NPL G N+E FG+RF  +SDAYD+ +R +A    +++GE + + EG Y 
Sbjct: 134 DHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYV 193

Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
           M  GP+FETV+EC ++Q  G D  GMSTV EVIVARH  + VF  S++T++ I++++S +
Sbjct: 194 MVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLE 253

Query: 727 TTCHKEVLKVSESSTKIVQKIITELIKEI 813
              H+EVL   + + + +++ ++ L+  I
Sbjct: 254 KANHEEVLAAGKQAAQKLEQFVSILMASI 282

>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  248 bits (633), Expect = 2e-65
 Identities = 116/236 (49%), Positives = 167/236 (70%)
 Frame = +1

Query: 106 IIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLM 285
           I  Y EI  FPQSTV GH G LVFG L G+  V+MQGRFH YEGY+      PVRV HL+
Sbjct: 47  IFDYNEIPNFPQSTVQGHAGRLVFGLLNGRCCVMMQGRFHMYEGYSLSKVTFPVRVFHLL 106

Query: 286 GVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPLSDAY 465
           GV+ L++TNAAGGLNP+++VGD+M+I+DHIN PG CG NPL G N+E FG RF  +SDAY
Sbjct: 107 GVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFPAMSDAY 166

Query: 466 DKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEVI 645
           D+++R KA    +++GE + + EG Y M  GP FETV+E  ++++ G D  GMSTV EVI
Sbjct: 167 DRDMRQKAFTAWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLKMLGADAVGMSTVPEVI 226

Query: 646 VARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEI 813
           VARH  + VF  S++T++ ++++++ +   H EVL   +++ + +++ ++ L++ I
Sbjct: 227 VARHCGLRVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVSILMESI 282

>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  243 bits (621), Expect = 4e-64
 Identities = 117/241 (48%), Positives = 165/241 (68%)
 Frame = +1

Query: 91  VTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVR 270
           +T      Y+EI  FP+STV GH G LVFG L G+  V+MQGRFH YEGY       PVR
Sbjct: 42  LTQAQTFDYSEIPNFPESTVPGHAGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVR 101

Query: 271 VMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIP 450
           V  L+GV+ L++TNAAGGLNP+++VGD+M+I+DHIN PG  G NPL G NEE FG RF  
Sbjct: 102 VFRLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPA 161

Query: 451 LSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMST 630
           +SDAYD+++R KA    +++GE + + EG Y M  GP FETV+EC +++  G D  GMST
Sbjct: 162 MSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMST 221

Query: 631 VHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKE 810
           V EVIVARH  + VF  S++T++ I++++S     H+EVL+  + + + +++ ++ L+  
Sbjct: 222 VPEVIVARHCGLRVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMAS 281

Query: 811 I 813
           I
Sbjct: 282 I 282

>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
           phosphorylase)
          Length = 274

 Score =  213 bits (541), Expect = 8e-55
 Identities = 116/270 (42%), Positives = 160/270 (59%)
 Frame = +1

Query: 7   AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
           A+F+  K    P+            A+ +   + IPY++I  FP STV GH G LV+G L
Sbjct: 10  AQFLKEKFPTSPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQLVYGQL 69

Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
            G  VV+MQGRFH YEGY+      PVRVM  +GV+ L++TNAAGG+N S++ GDLMII 
Sbjct: 70  EGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPGDLMIIS 129

Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
           DHIN     G NPL+G N+ + G RF  +S+AY K LR  AK++   +G    V EGVY 
Sbjct: 130 DHIN---NMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIGLR--VREGVYV 184

Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
             TGPA+ET +E  M+++ GGD  GMSTV EVIVARH  M V  +S +++      D   
Sbjct: 185 ANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISNMAAGILDQPL 244

Query: 727 TTCHKEVLKVSESSTKIVQKIITELIKEIA 816
           T  H EV++ +E       + +  +++ +A
Sbjct: 245 T--HDEVIETTEKVKADFLRFVKAIVRNMA 272

>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 311

 Score =  202 bits (513), Expect = 1e-51
 Identities = 113/251 (45%), Positives = 155/251 (61%), Gaps = 16/251 (6%)
 Frame = +1

Query: 109 IPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMG 288
           +PY +I GF +STV GH G L+FG++ G  VVLM GR H YEG T      P+RV++ MG
Sbjct: 65  VPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRLHGYEGNTLFETTFPIRVLNHMG 124

Query: 289 -VKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPLSDAY 465
            V+ L++TNAAGG+N  Y+  DLM I DH+N PGL G +PL G N +  G RF+ LSDAY
Sbjct: 125 HVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAY 184

Query: 466 DKNLRMKAKEIIEKLGESKI---VHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVH 636
           D  LR   K + +K  E KI   +HEG Y   +GP FET +E  M+++ GGD  GMSTV 
Sbjct: 185 DLELR---KLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVP 241

Query: 637 EVIVARHMNMHVFAMSIVTDECILE------------FDSNKTTCHKEVLKVSESSTKIV 780
           EVIVARH    V A+S++T+ C+++             +  K T H EVL+  + ++  V
Sbjct: 242 EVIVARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKAT-HAEVLENGKIASNDV 300

Query: 781 QKIITELIKEI 813
           Q +I  ++ E+
Sbjct: 301 QNLIAAVMGEL 311

>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
           phosphorylase)
          Length = 271

 Score =  196 bits (498), Expect = 8e-50
 Identities = 110/268 (41%), Positives = 153/268 (57%)
 Frame = +1

Query: 7   AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
           A FI   +   PK            A+ + + + + Y +I  FP STV GH G LV G L
Sbjct: 9   AAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTL 68

Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
            G  V+ MQGRFH YEGY+ +    PVRVM  +GV+ L++TNAAGG+N  ++ GDLMII 
Sbjct: 69  EGVSVIAMQGRFHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIIT 128

Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
           DHINF    G NPL+G NE  FG RF  +S AYDK+L   A++I + L     + +GVY 
Sbjct: 129 DHINF---MGTNPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDLNIP--IQKGVYT 183

Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
             TGP++ET +E   ++  G D  GMSTV EVIVA H  M V  +S +++      D  +
Sbjct: 184 AVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD--Q 241

Query: 727 TTCHKEVLKVSESSTKIVQKIITELIKE 810
              H EV++V+E       K++  ++ +
Sbjct: 242 PLSHDEVMEVTEKVKAGFLKLVKAIVAQ 269

>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
          Length = 277

 Score =  167 bits (423), Expect = 4e-41
 Identities = 101/246 (41%), Positives = 141/246 (57%), Gaps = 1/246 (0%)
 Frame = +1

Query: 82  AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
           A+ + + + I Y ++ GFP STV GH G LV G+L G  VV M+GR H YEG        
Sbjct: 40  ADQIENAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTD 99

Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHIN-FPGLCGFNPLVGHNEESFGE 438
            +R   L+G + L  TNAAG L P    G L+ +KDHIN  PG     P+VG N++ FGE
Sbjct: 100 AIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPG----TPMVGLNDDRFGE 155

Query: 439 RFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVC 618
           RF  L++AYD   R   +++ ++  E   + EGV+    GP FET +E  MMQI GGDV 
Sbjct: 156 RFFSLANAYDAEYRALLQKVAKE--EGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVV 213

Query: 619 GMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITE 798
           GMS V EVI ARH ++ V A+S +T+  + E  S+    H + L  +E S +    +I  
Sbjct: 214 GMSVVPEVISARHCDLKVVAVSAITN--MAEGLSDVKLSHAQTLAAAELSKQNFINLICG 271

Query: 799 LIKEIA 816
            +++IA
Sbjct: 272 FLRKIA 277

>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 268

 Score =  142 bits (359), Expect = 1e-33
 Identities = 85/241 (35%), Positives = 126/241 (52%)
 Frame = +1

Query: 82  AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
           A L + + + P  E+ GF      GH G L+   +G   V+++ GR HPYEG+  +  + 
Sbjct: 43  AALGSSRAVFPQAELPGFITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVH 102

Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGER 441
           PVR     G + ++LTNAAGGL     VG L++I DH+N   L   +PLV       G  
Sbjct: 103 PVRTACAAGARIIVLTNAAGGLRADMAVGQLVLISDHLN---LTTRSPLV-------GTH 152

Query: 442 FIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCG 621
           F+ L++AY   LR  A +          + EGVYA Q GP +ET +E  M+++ G D+ G
Sbjct: 153 FVDLTNAYTTRLRKLASD------TDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVG 206

Query: 622 MSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITEL 801
           MSTVHE I AR     V  +S+VT+  +    + K   H EVL    +S   +  ++ ++
Sbjct: 207 MSTVHETIAARAAGAEVLGVSLVTN--LAAGITGKPLNHAEVLAAGTASANRIGSLLADI 264

Query: 802 I 804
           I
Sbjct: 265 I 265

>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
 sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 268

 Score =  139 bits (349), Expect = 2e-32
 Identities = 87/241 (36%), Positives = 128/241 (53%)
 Frame = +1

Query: 82  AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
           A L +   ++P  E+ GF   T  GH G L+   +G   V+++ GR H YEG+  +  + 
Sbjct: 43  AALGSPTTVLPQAELPGFVPPTAAGHAGELLSVPIGAHRVLVLAGRIHAYEGHDLRYVVH 102

Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGER 441
           PVR     G + ++LTNAAGGL    +VG  ++I DH+N   L   +PLVG      GE 
Sbjct: 103 PVRAARAAGAQIMVLTNAAGGLRADLQVGQPVLISDHLN---LTARSPLVG------GE- 152

Query: 442 FIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCG 621
           F+ L+DAY   LR  A++   +L       EGVYA   GP +ET +E  M+Q  G D+ G
Sbjct: 153 FVDLTDAYSPRLRELARQSDPQLA------EGVYAGLPGPHYETPAEIRMLQTLGADLVG 206

Query: 622 MSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITEL 801
           MSTVHE I AR     V  +S+VT+  +    + +   H EVL    +S   +  ++ ++
Sbjct: 207 MSTVHETIAARAAGAEVLGVSLVTN--LAAGITGEPLSHAEVLAAGAASATRMGALLADV 264

Query: 802 I 804
           I
Sbjct: 265 I 265

>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 282

 Score =  103 bits (256), Expect = 9e-22
 Identities = 73/238 (30%), Positives = 110/238 (46%), Gaps = 5/238 (2%)
 Frame = +1

Query: 109 IPYTEIDGFPQSTVVGHKG-----NLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRV 273
           +P  EI GF    V GH        +   +   +  +++  R H YEG   +  +  VR 
Sbjct: 62  VPTHEIPGFSAPAVAGHLSVTRSIRVERADGSVRHALVLGSRTHLYEGKGVRAVVHGVRT 121

Query: 274 MHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPL 453
               G + L+LTN  GGLN  +  G  +++ DHIN   L   +PL G         F+ L
Sbjct: 122 AAATGAETLILTNGCGGLNQEWGAGTPVLLSDHIN---LTARSPLEGPT-------FVDL 171

Query: 454 SDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTV 633
           +D Y   LR  A  +   L E      GVYA   GP +ET +E  M  I G D+ GMST 
Sbjct: 172 TDVYSPRLRELAHRVDPTLPE------GVYAQFPGPHYETPAEVRMAGILGADLVGMSTT 225

Query: 634 HEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIK 807
            E I ARH  + V  +S+VT+  +    S     H EV++  +++   +  ++ ++ K
Sbjct: 226 LEAIAARHCGLEVLGVSLVTN--LAAGISPTPLSHAEVIEAGQAAGPRISALLADIAK 281

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,613,544
Number of Sequences: 369166
Number of extensions: 2388267
Number of successful extensions: 5792
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5774
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9904824765
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00110

  1. Dr_sW_003_M13
  2. Dr_sW_013_P05