Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_M13
(788 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosi... 246 4e-65
sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosi... 244 3e-64
sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosi... 240 4e-63
sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP... 211 2e-54
sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosi... 197 3e-50
sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP... 195 1e-49
sp|P45563|XAPA_ECOLI Xanthosine phosphorylase 164 3e-40
sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosi... 139 1e-32
sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosi... 137 4e-32
sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosi... 100 6e-21
>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 246 bits (629), Expect = 4e-65
Identities = 120/260 (46%), Positives = 170/260 (65%)
Frame = +1
Query: 7 AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
AE++ S +P+ + +T I Y+EI FP+STV GH G LVFG L
Sbjct: 14 AEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFL 73
Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
G+ V+MQGRFH YEGY PVRV HL+GV L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74 NGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIR 133
Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
DHIN PG G NPL G N+E FG+RF +SDAYD+ +R +A +++GE + + EG Y
Sbjct: 134 DHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYV 193
Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
M GP+FETV+EC ++Q G D GMSTV EVIVARH + VF S++T++ I++++S +
Sbjct: 194 MVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLE 253
Query: 727 TTCHKEVLKVSESSTKIVQK 786
H+EVL + + + +++
Sbjct: 254 KANHEEVLAAGKQAAQKLEQ 273
>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 244 bits (622), Expect = 3e-64
Identities = 114/227 (50%), Positives = 161/227 (70%)
Frame = +1
Query: 106 IIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLM 285
I Y EI FPQSTV GH G LVFG L G+ V+MQGRFH YEGY+ PVRV HL+
Sbjct: 47 IFDYNEIPNFPQSTVQGHAGRLVFGLLNGRCCVMMQGRFHMYEGYSLSKVTFPVRVFHLL 106
Query: 286 GVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPLSDAY 465
GV+ L++TNAAGGLNP+++VGD+M+I+DHIN PG CG NPL G N+E FG RF +SDAY
Sbjct: 107 GVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFPAMSDAY 166
Query: 466 DKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEVI 645
D+++R KA +++GE + + EG Y M GP FETV+E ++++ G D GMSTV EVI
Sbjct: 167 DRDMRQKAFTAWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLKMLGADAVGMSTVPEVI 226
Query: 646 VARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQK 786
VARH + VF S++T++ ++++++ + H EVL +++ + +++
Sbjct: 227 VARHCGLRVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLER 273
>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 240 bits (612), Expect = 4e-63
Identities = 115/232 (49%), Positives = 160/232 (68%)
Frame = +1
Query: 91 VTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVR 270
+T Y+EI FP+STV GH G LVFG L G+ V+MQGRFH YEGY PVR
Sbjct: 42 LTQAQTFDYSEIPNFPESTVPGHAGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVR 101
Query: 271 VMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIP 450
V L+GV+ L++TNAAGGLNP+++VGD+M+I+DHIN PG G NPL G NEE FG RF
Sbjct: 102 VFRLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPA 161
Query: 451 LSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMST 630
+SDAYD+++R KA +++GE + + EG Y M GP FETV+EC +++ G D GMST
Sbjct: 162 MSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMST 221
Query: 631 VHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQK 786
V EVIVARH + VF S++T++ I++++S H+EVL+ + + + +++
Sbjct: 222 VPEVIVARHCGLRVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQ 273
>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 274
Score = 211 bits (536), Expect = 2e-54
Identities = 115/252 (45%), Positives = 153/252 (60%)
Frame = +1
Query: 7 AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
A+F+ K P+ A+ + + IPY++I FP STV GH G LV+G L
Sbjct: 10 AQFLKEKFPTSPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQLVYGQL 69
Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
G VV+MQGRFH YEGY+ PVRVM +GV+ L++TNAAGG+N S++ GDLMII
Sbjct: 70 EGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPGDLMIIS 129
Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
DHIN G NPL+G N+ + G RF +S+AY K LR AK++ +G V EGVY
Sbjct: 130 DHIN---NMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIGLR--VREGVYV 184
Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
TGPA+ET +E M+++ GGD GMSTV EVIVARH M V +S +++ D
Sbjct: 185 ANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISNMAAGILDQPL 244
Query: 727 TTCHKEVLKVSE 762
T H EV++ +E
Sbjct: 245 T--HDEVIETTE 254
>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 311
Score = 197 bits (501), Expect = 3e-50
Identities = 111/241 (46%), Positives = 149/241 (61%), Gaps = 16/241 (6%)
Frame = +1
Query: 109 IPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMG 288
+PY +I GF +STV GH G L+FG++ G VVLM GR H YEG T P+RV++ MG
Sbjct: 65 VPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRLHGYEGNTLFETTFPIRVLNHMG 124
Query: 289 -VKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPLSDAY 465
V+ L++TNAAGG+N Y+ DLM I DH+N PGL G +PL G N + G RF+ LSDAY
Sbjct: 125 HVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAY 184
Query: 466 DKNLRMKAKEIIEKLGESKI---VHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVH 636
D LR K + +K E KI +HEG Y +GP FET +E M+++ GGD GMSTV
Sbjct: 185 DLELR---KLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVP 241
Query: 637 EVIVARHMNMHVFAMSIVTDECILE------------FDSNKTTCHKEVLKVSESSTKIV 780
EVIVARH V A+S++T+ C+++ + K T H EVL+ + ++ V
Sbjct: 242 EVIVARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKAT-HAEVLENGKIASNDV 300
Query: 781 Q 783
Q
Sbjct: 301 Q 301
>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 271
Score = 195 bits (495), Expect = 1e-49
Identities = 109/252 (43%), Positives = 147/252 (58%)
Frame = +1
Query: 7 AEFINSKITIKPKXXXXXXXXXXXXAELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNL 186
A FI + PK A+ + + + + Y +I FP STV GH G LV G L
Sbjct: 9 AAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTL 68
Query: 187 GGKEVVLMQGRFHPYEGYTTQTCIIPVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIK 366
G V+ MQGRFH YEGY+ + PVRVM +GV+ L++TNAAGG+N ++ GDLMII
Sbjct: 69 EGVSVIAMQGRFHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIIT 128
Query: 367 DHINFPGLCGFNPLVGHNEESFGERFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYA 546
DHINF G NPL+G NE FG RF +S AYDK+L A++I + L + +GVY
Sbjct: 129 DHINF---MGTNPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDLNIP--IQKGVYT 183
Query: 547 MQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNK 726
TGP++ET +E ++ G D GMSTV EVIVA H M V +S +++ D +
Sbjct: 184 AVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD--Q 241
Query: 727 TTCHKEVLKVSE 762
H EV++V+E
Sbjct: 242 PLSHDEVMEVTE 253
>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
Length = 277
Score = 164 bits (414), Expect = 3e-40
Identities = 98/230 (42%), Positives = 133/230 (57%), Gaps = 1/230 (0%)
Frame = +1
Query: 82 AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
A+ + + + I Y ++ GFP STV GH G LV G+L G VV M+GR H YEG
Sbjct: 40 ADQIENAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTD 99
Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHIN-FPGLCGFNPLVGHNEESFGE 438
+R L+G + L TNAAG L P G L+ +KDHIN PG P+VG N++ FGE
Sbjct: 100 AIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPG----TPMVGLNDDRFGE 155
Query: 439 RFIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVC 618
RF L++AYD R +++ ++ E + EGV+ GP FET +E MMQI GGDV
Sbjct: 156 RFFSLANAYDAEYRALLQKVAKE--EGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVV 213
Query: 619 GMSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESS 768
GMS V EVI ARH ++ V A+S +T+ + E S+ H + L +E S
Sbjct: 214 GMSVVPEVISARHCDLKVVAVSAITN--MAEGLSDVKLSHAQTLAAAELS 261
>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 139 bits (349), Expect = 1e-32
Identities = 84/229 (36%), Positives = 120/229 (52%)
Frame = +1
Query: 82 AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
A L + + + P E+ GF GH G L+ +G V+++ GR HPYEG+ + +
Sbjct: 43 AALGSSRAVFPQAELPGFITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVH 102
Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGER 441
PVR G + ++LTNAAGGL VG L++I DH+N L +PLV G
Sbjct: 103 PVRTACAAGARIIVLTNAAGGLRADMAVGQLVLISDHLN---LTTRSPLV-------GTH 152
Query: 442 FIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCG 621
F+ L++AY LR A + + EGVYA Q GP +ET +E M+++ G D+ G
Sbjct: 153 FVDLTNAYTTRLRKLASD------TDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVG 206
Query: 622 MSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESS 768
MSTVHE I AR V +S+VT+ + + K H EVL +S
Sbjct: 207 MSTVHETIAARAAGAEVLGVSLVTN--LAAGITGKPLNHAEVLAAGTAS 253
>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 137 bits (344), Expect = 4e-32
Identities = 86/229 (37%), Positives = 122/229 (53%)
Frame = +1
Query: 82 AELVTDKLIIPYTEIDGFPQSTVVGHKGNLVFGNLGGKEVVLMQGRFHPYEGYTTQTCII 261
A L + ++P E+ GF T GH G L+ +G V+++ GR H YEG+ + +
Sbjct: 43 AALGSPTTVLPQAELPGFVPPTAAGHAGELLSVPIGAHRVLVLAGRIHAYEGHDLRYVVH 102
Query: 262 PVRVMHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGER 441
PVR G + ++LTNAAGGL +VG ++I DH+N L +PLVG GE
Sbjct: 103 PVRAARAAGAQIMVLTNAAGGLRADLQVGQPVLISDHLN---LTARSPLVG------GE- 152
Query: 442 FIPLSDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCG 621
F+ L+DAY LR A++ +L EGVYA GP +ET +E M+Q G D+ G
Sbjct: 153 FVDLTDAYSPRLRELARQSDPQLA------EGVYAGLPGPHYETPAEIRMLQTLGADLVG 206
Query: 622 MSTVHEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESS 768
MSTVHE I AR V +S+VT+ + + + H EVL +S
Sbjct: 207 MSTVHETIAARAAGAEVLGVSLVTN--LAAGITGEPLSHAEVLAAGAAS 253
>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 282
Score = 100 bits (248), Expect = 6e-21
Identities = 72/225 (32%), Positives = 104/225 (46%), Gaps = 5/225 (2%)
Frame = +1
Query: 109 IPYTEIDGFPQSTVVGHKG-----NLVFGNLGGKEVVLMQGRFHPYEGYTTQTCIIPVRV 273
+P EI GF V GH + + + +++ R H YEG + + VR
Sbjct: 62 VPTHEIPGFSAPAVAGHLSVTRSIRVERADGSVRHALVLGSRTHLYEGKGVRAVVHGVRT 121
Query: 274 MHLMGVKYLLLTNAAGGLNPSYKVGDLMIIKDHINFPGLCGFNPLVGHNEESFGERFIPL 453
G + L+LTN GGLN + G +++ DHIN L +PL G F+ L
Sbjct: 122 AAATGAETLILTNGCGGLNQEWGAGTPVLLSDHIN---LTARSPLEGPT-------FVDL 171
Query: 454 SDAYDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTV 633
+D Y LR A + L E GVYA GP +ET +E M I G D+ GMST
Sbjct: 172 TDVYSPRLRELAHRVDPTLPE------GVYAQFPGPHYETPAEVRMAGILGADLVGMSTT 225
Query: 634 HEVIVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESS 768
E I ARH + V +S+VT+ + S H EV++ +++
Sbjct: 226 LEAIAARHCGLEVLGVSLVTN--LAAGISPTPLSHAEVIEAGQAA 268
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,201,314
Number of Sequences: 369166
Number of extensions: 2103367
Number of successful extensions: 5256
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5240
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)