Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_P05
(495 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosi... 100 3e-21
sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosi... 99 4e-21
sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosi... 96 5e-20
sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosi... 80 3e-15
sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP... 80 3e-15
sp|P45563|XAPA_ECOLI Xanthosine phosphorylase 69 6e-12
sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP... 66 4e-11
sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosi... 65 1e-10
sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosi... 60 4e-09
sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosi... 58 1e-08
>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 99.8 bits (247), Expect = 3e-21
Identities = 45/117 (38%), Positives = 77/117 (65%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
YD+ +R +A +++GE + + EG Y M GP+FETV+EC ++Q G D GMSTV EV
Sbjct: 166 YDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEV 225
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEI 352
IVARH + VF S++T++ I++++S + H+EVL + + + +++ ++ L+ I
Sbjct: 226 IVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASI 282
>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 99.4 bits (246), Expect = 4e-21
Identities = 45/117 (38%), Positives = 77/117 (65%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
YD+++R KA +++GE + + EG Y M GP FETV+EC +++ G D GMSTV EV
Sbjct: 166 YDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEV 225
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEI 352
IVARH + VF S++T++ I++++S H+EVL+ + + + +++ ++ L+ I
Sbjct: 226 IVARHCGLRVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASI 282
>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 95.9 bits (237), Expect = 5e-20
Identities = 42/117 (35%), Positives = 78/117 (66%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
YD+++R KA +++GE + + EG Y M GP FETV+E ++++ G D GMSTV EV
Sbjct: 166 YDRDMRQKAFTAWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLKMLGADAVGMSTVPEV 225
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEI 352
IVARH + VF S++T++ ++++++ + H EVL +++ + +++ ++ L++ I
Sbjct: 226 IVARHCGLRVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVSILMESI 282
>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 311
Score = 80.1 bits (196), Expect = 3e-15
Identities = 50/132 (37%), Positives = 74/132 (56%), Gaps = 15/132 (11%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKI---VHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTV 172
YD LR K + +K E KI +HEG Y +GP FET +E M+++ GGD GMSTV
Sbjct: 184 YDLELR---KLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTV 240
Query: 173 HEVIVARHMNMHVFAMSIVTDECILE------------FDSNKTTCHKEVLKVSESSTKI 316
EVIVARH V A+S++T+ C+++ + K T H EVL+ + ++
Sbjct: 241 PEVIVARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKAT-HAEVLENGKIASND 299
Query: 317 VQKIITELIKEI 352
VQ +I ++ E+
Sbjct: 300 VQNLIAAVMGEL 311
>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 274
Score = 80.1 bits (196), Expect = 3e-15
Identities = 45/118 (38%), Positives = 66/118 (55%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
Y K LR AK++ +G V EGVY TGPA+ET +E M+++ GGD GMSTV EV
Sbjct: 159 YSKRLRQLAKDVANDIGLR--VREGVYVANTGPAYETPAEIRMIRVMGGDAVGMSTVPEV 216
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEIA 355
IVARH M V +S +++ D T H EV++ +E + + +++ +A
Sbjct: 217 IVARHAGMEVLGISCISNMAAGILDQPLT--HDEVIETTEKVKADFLRFVKAIVRNMA 272
>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
Length = 277
Score = 68.9 bits (167), Expect = 6e-12
Identities = 44/118 (37%), Positives = 66/118 (55%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
YD R +++ ++ G + EGV+ GP FET +E MMQI GGDV GMS V EV
Sbjct: 164 YDAEYRALLQKVAKEEGFP--LTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEV 221
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKEIA 355
I ARH ++ V A+S +T+ + E S+ H + L +E S + +I +++IA
Sbjct: 222 ISARHCDLKVVAVSAITN--MAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKIA 277
>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 271
Score = 66.2 bits (160), Expect = 4e-11
Identities = 39/116 (33%), Positives = 63/116 (54%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
YDK+L A++I + L + +GVY TGP++ET +E ++ G D GMSTV EV
Sbjct: 158 YDKDLSSLAEKIAKDLNIP--IQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEV 215
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELIKE 349
IVA H M V +S +++ D + H EV++V+E K++ ++ +
Sbjct: 216 IVANHAGMRVLGISCISNAAAGILD--QPLSHDEVMEVTEKVKAGFLKLVKAIVAQ 269
>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 64.7 bits (156), Expect = 1e-10
Identities = 35/91 (38%), Positives = 52/91 (57%)
Frame = +2
Query: 71 EGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILE 250
EGVYA Q GP +ET +E M+++ G D+ GMSTVHE I AR V +S+VT+ +
Sbjct: 177 EGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTN--LAA 234
Query: 251 FDSNKTTCHKEVLKVSESSTKIVQKIITELI 343
+ K H EVL +S + ++ ++I
Sbjct: 235 GITGKPLNHAEVLAAGTASANRIGSLLADII 265
>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 59.7 bits (143), Expect = 4e-09
Identities = 39/114 (34%), Positives = 58/114 (50%)
Frame = +2
Query: 2 YDKNLRMKAKEIIEKLGESKIVHEGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEV 181
Y LR A++ +L E GVYA GP +ET +E M+Q G D+ GMSTVHE
Sbjct: 160 YSPRLRELARQSDPQLAE------GVYAGLPGPHYETPAEIRMLQTLGADLVGMSTVHET 213
Query: 182 IVARHMNMHVFAMSIVTDECILEFDSNKTTCHKEVLKVSESSTKIVQKIITELI 343
I AR V +S+VT+ + + + H EVL +S + ++ ++I
Sbjct: 214 IAARAAGAEVLGVSLVTN--LAAGITGEPLSHAEVLAAGAASATRMGALLADVI 265
>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 282
Score = 58.2 bits (139), Expect = 1e-08
Identities = 32/92 (34%), Positives = 50/92 (54%)
Frame = +2
Query: 71 EGVYAMQTGPAFETVSECLMMQICGGDVCGMSTVHEVIVARHMNMHVFAMSIVTDECILE 250
EGVYA GP +ET +E M I G D+ GMST E I ARH + V +S+VT+ +
Sbjct: 192 EGVYAQFPGPHYETPAEVRMAGILGADLVGMSTTLEAIAARHCGLEVLGVSLVTN--LAA 249
Query: 251 FDSNKTTCHKEVLKVSESSTKIVQKIITELIK 346
S H EV++ +++ + ++ ++ K
Sbjct: 250 GISPTPLSHAEVIEAGQAAGPRISALLADIAK 281
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,703,729
Number of Sequences: 369166
Number of extensions: 1088125
Number of successful extensions: 3112
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2923
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3109
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3069744620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)