Planaria EST Database

DrC_00087

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00087
         (969 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q99JX3|GORS2_MOUSE  Golgi reassembly stacking protein 2 (...   260   5e-69
sp|Q9H8Y8|GORS2_HUMAN  Golgi reassembly stacking protein 2 (...   259   8e-69
sp|Q9R064|GORS2_RAT  Golgi reassembly stacking protein 2 (GR...   259   8e-69
sp|O35254|GR65_RAT  Golgi reassembly stacking protein 1 (Gol...   238   2e-62
sp|Q91X51|GR65_MOUSE  Golgi reassembly stacking protein 1 (G...   236   7e-62
sp|Q9BQQ3|GR65_HUMAN  Golgi reassembly stacking protein 1 (G...   234   2e-61
sp|Q10149|YAT2_SCHPO  Hypothetical protein C1D4.02c in chrom...   101   3e-21
sp|P40555|PSD9_YEAST  Probable 26S proteasome regulatory sub...    38   0.049
sp|O00233|PSD9_HUMAN  26S proteasome non-ATPase regulatory s...    38   0.049
sp|Q9CR00|PSD9_MOUSE  26S proteasome non-ATPase regulatory s...    37   0.064

>sp|Q99JX3|GORS2_MOUSE Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
           stacking protein of 55 kDa) (GRASP55)
          Length = 451

 Score =  260 bits (664), Expect = 5e-69
 Identities = 127/243 (52%), Positives = 167/243 (68%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG+  S      G EGYHVLRVQE SPGH+AGL+ FFDF+V++  ++L++DN+ LK+LLK
Sbjct: 1   MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLK 60

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ + I++SKT   R   + PS  WGGQGLLGVSIRFCSF+GANENVWHVL+++ 
Sbjct: 61  ANVEKPVKMLIYSSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           NSPAALAGL+  +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSINNGCCETHSCEKNLHDVNNALDGFSEI 777
           NS W               HRIP+ PF     I+     T +       +    DGF+E+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKISLPGQMTGT------PITPLKDGFTEV 234

Query: 778 ALT 786
            L+
Sbjct: 235 QLS 237

>sp|Q9H8Y8|GORS2_HUMAN Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
           stacking protein of 55 kDa) (GRASP55) (p59) (Golgi
           phosphoprotein 6) (GOLPH6)
          Length = 452

 Score =  259 bits (662), Expect = 8e-69
 Identities = 121/214 (56%), Positives = 156/214 (72%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG+  S      G EGYHVLRVQE SPGH+AGL+ FFDF+V++  ++L++DN+ LK+LLK
Sbjct: 1   MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLK 60

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ + I++SKT   R   + PS  WGGQGLLGVSIRFCSF+GANENVWHVL+++ 
Sbjct: 61  ANVEKPVKMLIYSSKTLELRETSVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           NSPAALAGL+  +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSIN 699
           NS W               HRIP+ PF     I+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKIS 214

>sp|Q9R064|GORS2_RAT Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
           stacking protein of 55 kDa) (GRASP55)
          Length = 454

 Score =  259 bits (662), Expect = 8e-69
 Identities = 121/214 (56%), Positives = 156/214 (72%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG+  S      G EGYHVLRVQE SPGH+AGL+ FFDF+V++  ++L++DN+ LK+LLK
Sbjct: 1   MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSISGSRLNKDNDTLKDLLK 60

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ + I++SKT   R   + PS  WGGQGLLGVSIRFCSF+GANENVWHVL+++ 
Sbjct: 61  ANVEKPVKMLIYSSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           NSPAALAGL+  +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSIN 699
           NS W               HRIP+ PF     I+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKIS 214

>sp|O35254|GR65_RAT Golgi reassembly stacking protein 1 (Golgi reassembly stacking
           protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
           protein p65)
          Length = 451

 Score =  238 bits (606), Expect = 2e-62
 Identities = 109/208 (52%), Positives = 150/208 (72%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG G+S      G EG+H+  VQE SP  +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1   MGLGASS-EQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 59

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ L +FN KT   R VE+VPS  WGGQGLLG S+RFCSF  A+E+VWHVLD++P
Sbjct: 60  ANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEP 119

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           +SPAALAGL+  TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TP
Sbjct: 120 SSPAALAGLRPYTDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTP 179

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFS 681
           N+ W               HRIP+ P S
Sbjct: 180 NAAWGGEGSLGCGIGYGYLHRIPTQPSS 207

>sp|Q91X51|GR65_MOUSE Golgi reassembly stacking protein 1 (Golgi reassembly stacking
           protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
           protein p65)
          Length = 446

 Score =  236 bits (602), Expect = 7e-62
 Identities = 109/208 (52%), Positives = 149/208 (71%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG G+S      G EG+H+  VQE SP  +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1   MGLGASS-EQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 59

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ L +FN KT   R VE+VPS  WGGQGLLG S+RFCSF  A+E+VWHVLD++P
Sbjct: 60  ANVEKPVKLEVFNMKTMKVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEP 119

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           +SPAALAGL   TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TP
Sbjct: 120 SSPAALAGLCPYTDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTP 179

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFS 681
           N+ W               HRIP+ P S
Sbjct: 180 NAAWGGEGSLGCGIGYGYLHRIPTQPSS 207

>sp|Q9BQQ3|GR65_HUMAN Golgi reassembly stacking protein 1 (Golgi reassembly stacking
           protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
           protein p65) (Golgi phosphoprotein 5) (GOLPH5)
          Length = 440

 Score =  234 bits (598), Expect = 2e-61
 Identities = 106/206 (51%), Positives = 147/206 (71%)
 Frame = +1

Query: 58  MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
           MG G S      G EG+H+  VQE SP  +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1   MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 60

Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
           +N +KP+ L +FN KT   R VE+VPS  WGGQGLLG S+RFCSF  A+E VWHVLD++P
Sbjct: 61  ANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEP 120

Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
           +SPAALAGL+  TDY++G++ +L + E+FF LIE+H+G+ LKL VYN ++D+CREVT+TP
Sbjct: 121 SSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTP 180

Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLP 675
           N+ W               HRIP+ P
Sbjct: 181 NAAWGGEGSLGCGIGYGYLHRIPTQP 206

>sp|Q10149|YAT2_SCHPO Hypothetical protein C1D4.02c in chromosome I
          Length = 345

 Score =  101 bits (252), Expect = 3e-21
 Identities = 58/198 (29%), Positives = 104/198 (52%), Gaps = 3/198 (1%)
 Frame = +1

Query: 85  HNHGNE--GYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPI 258
           H   +E  G+ VL+V+  S  + A +++++DF+ A+    L+ D  +   LL+ +  + +
Sbjct: 20  HRESDESCGFRVLKVENDSKAYNARIESYYDFITAVNGILLNGDPSMFMALLRDSSPE-V 78

Query: 259 NLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALA 438
            L +F+ K Q  R V +  + D      +G+ +++ S   A + +WH+L++  +SP A A
Sbjct: 79  TLEVFSLKGQITRKVNIKINSD----EKIGMVLQWASIAPAVDAIWHILNVIDDSPVARA 134

Query: 439 GLKQMTDYIIGA-ETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXX 615
            L    DYI+G  E ++   +   +LIE+H  + L+LY+YN   D+ R+VTI PN  W  
Sbjct: 135 SLVPYEDYIVGTPEGMMTGEKALSDLIESHLNRPLRLYIYNHYRDSTRQVTIVPNRHWGG 194

Query: 616 XXXXXXXXXXXXXHRIPS 669
                        HR+P+
Sbjct: 195 NGAIGCGVGHGVLHRLPA 212

>sp|P40555|PSD9_YEAST Probable 26S proteasome regulatory subunit p27 (Proteasome
           non-ATPase subunit 2)
          Length = 220

 Score = 37.7 bits (86), Expect = 0.049
 Identities = 21/85 (24%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
 Frame = +1

Query: 112 VLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEV--LKELLKSNKDKPINLTIFNSKT 285
           +  V  GSP  KA ++   D ++++G+   +  +++  ++ ++  N+D+P+ + +   + 
Sbjct: 137 ISEVVPGSPSDKADIKVD-DKLISIGNVHAANHSKLQNIQMVVMKNEDRPLPVLLL--RE 193

Query: 286 QSFRNVELVPSQDWGGQGLLGVSIR 360
                  L PS++W G+GLLG  I+
Sbjct: 194 GQILKTSLTPSRNWNGRGLLGCRIQ 218

>sp|O00233|PSD9_HUMAN 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
           regulatory subunit p27)
          Length = 223

 Score = 37.7 bits (86), Expect = 0.049
 Identities = 26/81 (32%), Positives = 45/81 (55%), Gaps = 2/81 (2%)
 Frame = +1

Query: 121 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 294
           +  GSP   AGLQ   D +V  G  +T+  Q    +  +++ ++ KP+N+T+     +  
Sbjct: 142 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSLHNIGSVVQHSEGKPLNVTVIRRGEK-- 198

Query: 295 RNVELVPSQDWGGQGLLGVSI 357
             + LVP++ W G+GLLG +I
Sbjct: 199 HQLRLVPTR-WAGKGLLGCNI 218

>sp|Q9CR00|PSD9_MOUSE 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
           regulatory subunit p27)
          Length = 222

 Score = 37.4 bits (85), Expect = 0.064
 Identities = 25/81 (30%), Positives = 46/81 (56%), Gaps = 2/81 (2%)
 Frame = +1

Query: 121 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 294
           +  GSP   AGLQ   D +V  G  +T+  Q  + +  +++ ++ KP+N+T+     +  
Sbjct: 141 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSVQNVGTVVQHSEGKPLNVTVIRRGEK-- 197

Query: 295 RNVELVPSQDWGGQGLLGVSI 357
             + L+P++ W G+GLLG +I
Sbjct: 198 HQLRLIPTR-WAGKGLLGCNI 217

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.317    0.134    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 108,722,477
Number of Sequences: 369166
Number of extensions: 2255554
Number of successful extensions: 6459
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6432
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10096222345
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)

Cluster detail

DrC_00087

  1. Dr_sW_003_E20
  2. Dr_sW_003_E10
  3. Dr_sW_018_O06