Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_E10
(860 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99JX3|GORS2_MOUSE Golgi reassembly stacking protein 2 (... 260 4e-69
sp|Q9H8Y8|GORS2_HUMAN Golgi reassembly stacking protein 2 (... 259 7e-69
sp|Q9R064|GORS2_RAT Golgi reassembly stacking protein 2 (GR... 259 7e-69
sp|O35254|GR65_RAT Golgi reassembly stacking protein 1 (Gol... 238 2e-62
sp|Q91X51|GR65_MOUSE Golgi reassembly stacking protein 1 (G... 236 6e-62
sp|Q9BQQ3|GR65_HUMAN Golgi reassembly stacking protein 1 (G... 234 2e-61
sp|Q10149|YAT2_SCHPO Hypothetical protein C1D4.02c in chrom... 101 2e-21
sp|P40555|PSD9_YEAST Probable 26S proteasome regulatory sub... 38 0.041
sp|O00233|PSD9_HUMAN 26S proteasome non-ATPase regulatory s... 38 0.041
sp|Q9CR00|PSD9_MOUSE 26S proteasome non-ATPase regulatory s... 37 0.054
>sp|Q99JX3|GORS2_MOUSE Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55)
Length = 451
Score = 260 bits (664), Expect = 4e-69
Identities = 127/243 (52%), Positives = 167/243 (68%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG+ S G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK
Sbjct: 1 MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLK 60
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ + I++SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++
Sbjct: 61 ANVEKPVKMLIYSSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
NSPAALAGL+ +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSINNGCCETHSCEKNLHDVNNALDGFSEI 777
NS W HRIP+ PF I+ T + + DGF+E+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKISLPGQMTGT------PITPLKDGFTEV 234
Query: 778 ALT 786
L+
Sbjct: 235 QLS 237
>sp|Q9H8Y8|GORS2_HUMAN Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55) (p59) (Golgi
phosphoprotein 6) (GOLPH6)
Length = 452
Score = 259 bits (662), Expect = 7e-69
Identities = 121/214 (56%), Positives = 156/214 (72%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG+ S G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK
Sbjct: 1 MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLK 60
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ + I++SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++
Sbjct: 61 ANVEKPVKMLIYSSKTLELRETSVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
NSPAALAGL+ +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSIN 699
NS W HRIP+ PF I+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKIS 214
>sp|Q9R064|GORS2_RAT Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55)
Length = 454
Score = 259 bits (662), Expect = 7e-69
Identities = 121/214 (56%), Positives = 156/214 (72%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG+ S G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK
Sbjct: 1 MGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSISGSRLNKDNDTLKDLLK 60
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ + I++SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++
Sbjct: 61 ANVEKPVKMLIYSSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVES 120
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
NSPAALAGL+ +DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITP
Sbjct: 121 NSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITP 180
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFSNISSIN 699
NS W HRIP+ PF I+
Sbjct: 181 NSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKIS 214
>sp|O35254|GR65_RAT Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65)
Length = 451
Score = 238 bits (606), Expect = 2e-62
Identities = 109/208 (52%), Positives = 150/208 (72%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG G+S G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1 MGLGASS-EQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 59
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ L +FN KT R VE+VPS WGGQGLLG S+RFCSF A+E+VWHVLD++P
Sbjct: 60 ANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEP 119
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
+SPAALAGL+ TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TP
Sbjct: 120 SSPAALAGLRPYTDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTP 179
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFS 681
N+ W HRIP+ P S
Sbjct: 180 NAAWGGEGSLGCGIGYGYLHRIPTQPSS 207
>sp|Q91X51|GR65_MOUSE Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65)
Length = 446
Score = 236 bits (602), Expect = 6e-62
Identities = 109/208 (52%), Positives = 149/208 (71%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG G+S G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1 MGLGASS-EQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 59
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ L +FN KT R VE+VPS WGGQGLLG S+RFCSF A+E+VWHVLD++P
Sbjct: 60 ANVEKPVKLEVFNMKTMKVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEP 119
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
+SPAALAGL TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TP
Sbjct: 120 SSPAALAGLCPYTDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTP 179
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLPFS 681
N+ W HRIP+ P S
Sbjct: 180 NAAWGGEGSLGCGIGYGYLHRIPTQPSS 207
>sp|Q9BQQ3|GR65_HUMAN Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65) (Golgi phosphoprotein 5) (GOLPH5)
Length = 440
Score = 234 bits (598), Expect = 2e-61
Identities = 106/206 (51%), Positives = 147/206 (71%)
Frame = +1
Query: 58 MGAGSSDLSHNHGNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLK 237
MG G S G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK
Sbjct: 1 MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLK 60
Query: 238 SNKDKPINLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDP 417
+N +KP+ L +FN KT R VE+VPS WGGQGLLG S+RFCSF A+E VWHVLD++P
Sbjct: 61 ANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEP 120
Query: 418 NSPAALAGLKQMTDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITP 597
+SPAALAGL+ TDY++G++ +L + E+FF LIE+H+G+ LKL VYN ++D+CREVT+TP
Sbjct: 121 SSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTP 180
Query: 598 NSTWXXXXXXXXXXXXXXXHRIPSLP 675
N+ W HRIP+ P
Sbjct: 181 NAAWGGEGSLGCGIGYGYLHRIPTQP 206
>sp|Q10149|YAT2_SCHPO Hypothetical protein C1D4.02c in chromosome I
Length = 345
Score = 101 bits (252), Expect = 2e-21
Identities = 58/198 (29%), Positives = 104/198 (52%), Gaps = 3/198 (1%)
Frame = +1
Query: 85 HNHGNE--GYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPI 258
H +E G+ VL+V+ S + A +++++DF+ A+ L+ D + LL+ + + +
Sbjct: 20 HRESDESCGFRVLKVENDSKAYNARIESYYDFITAVNGILLNGDPSMFMALLRDSSPE-V 78
Query: 259 NLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALA 438
L +F+ K Q R V + + D +G+ +++ S A + +WH+L++ +SP A A
Sbjct: 79 TLEVFSLKGQITRKVNIKINSD----EKIGMVLQWASIAPAVDAIWHILNVIDDSPVARA 134
Query: 439 GLKQMTDYIIGA-ETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXX 615
L DYI+G E ++ + +LIE+H + L+LY+YN D+ R+VTI PN W
Sbjct: 135 SLVPYEDYIVGTPEGMMTGEKALSDLIESHLNRPLRLYIYNHYRDSTRQVTIVPNRHWGG 194
Query: 616 XXXXXXXXXXXXXHRIPS 669
HR+P+
Sbjct: 195 NGAIGCGVGHGVLHRLPA 212
>sp|P40555|PSD9_YEAST Probable 26S proteasome regulatory subunit p27 (Proteasome
non-ATPase subunit 2)
Length = 220
Score = 37.7 bits (86), Expect = 0.041
Identities = 21/85 (24%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
Frame = +1
Query: 112 VLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEV--LKELLKSNKDKPINLTIFNSKT 285
+ V GSP KA ++ D ++++G+ + +++ ++ ++ N+D+P+ + + +
Sbjct: 137 ISEVVPGSPSDKADIKVD-DKLISIGNVHAANHSKLQNIQMVVMKNEDRPLPVLLL--RE 193
Query: 286 QSFRNVELVPSQDWGGQGLLGVSIR 360
L PS++W G+GLLG I+
Sbjct: 194 GQILKTSLTPSRNWNGRGLLGCRIQ 218
>sp|O00233|PSD9_HUMAN 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
regulatory subunit p27)
Length = 223
Score = 37.7 bits (86), Expect = 0.041
Identities = 26/81 (32%), Positives = 45/81 (55%), Gaps = 2/81 (2%)
Frame = +1
Query: 121 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 294
+ GSP AGLQ D +V G +T+ Q + +++ ++ KP+N+T+ +
Sbjct: 142 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSLHNIGSVVQHSEGKPLNVTVIRRGEK-- 198
Query: 295 RNVELVPSQDWGGQGLLGVSI 357
+ LVP++ W G+GLLG +I
Sbjct: 199 HQLRLVPTR-WAGKGLLGCNI 218
>sp|Q9CR00|PSD9_MOUSE 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
regulatory subunit p27)
Length = 222
Score = 37.4 bits (85), Expect = 0.054
Identities = 25/81 (30%), Positives = 46/81 (56%), Gaps = 2/81 (2%)
Frame = +1
Query: 121 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 294
+ GSP AGLQ D +V G +T+ Q + + +++ ++ KP+N+T+ +
Sbjct: 141 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSVQNVGTVVQHSEGKPLNVTVIRRGEK-- 197
Query: 295 RNVELVPSQDWGGQGLLGVSI 357
+ L+P++ W G+GLLG +I
Sbjct: 198 HQLRLIPTR-WAGKGLLGCNI 217
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,563,929
Number of Sequences: 369166
Number of extensions: 2000384
Number of successful extensions: 5360
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5335
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)