Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_O06
(892 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99JX3|GORS2_MOUSE Golgi reassembly stacking protein 2 (... 258 1e-68
sp|Q9H8Y8|GORS2_HUMAN Golgi reassembly stacking protein 2 (... 258 2e-68
sp|Q9R064|GORS2_RAT Golgi reassembly stacking protein 2 (GR... 258 2e-68
sp|O35254|GR65_RAT Golgi reassembly stacking protein 1 (Gol... 236 8e-62
sp|Q91X51|GR65_MOUSE Golgi reassembly stacking protein 1 (G... 234 2e-61
sp|Q9BQQ3|GR65_HUMAN Golgi reassembly stacking protein 1 (G... 232 1e-60
sp|Q10149|YAT2_SCHPO Hypothetical protein C1D4.02c in chrom... 101 2e-21
sp|P40555|PSD9_YEAST Probable 26S proteasome regulatory sub... 38 0.043
sp|O00233|PSD9_HUMAN 26S proteasome non-ATPase regulatory s... 38 0.043
sp|Q9CR00|PSD9_MOUSE 26S proteasome non-ATPase regulatory s... 37 0.057
>sp|Q99JX3|GORS2_MOUSE Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55)
Length = 451
Score = 258 bits (660), Expect = 1e-68
Identities = 124/231 (53%), Positives = 163/231 (70%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK+N +KP+ + I+
Sbjct: 13 GTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLKANVEKPVKMLIY 72
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
+SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++ NSPAALAGL+
Sbjct: 73 SSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVESNSPAALAGLRPH 132
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
+DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITPNS W
Sbjct: 133 SDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGC 192
Query: 557 XXXXXXXHRIPSLPFSNISSINNGCCETHSCEKNLHDVNNALDGFSEIALT 709
HRIP+ PF I+ T + + DGF+E+ L+
Sbjct: 193 GIGYGYLHRIPTRPFEEGKKISLPGQMTGT------PITPLKDGFTEVQLS 237
Score = 45.4 bits (106), Expect = 2e-04
Identities = 23/80 (28%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = +2
Query: 299 GANENVWHVLDIDPNSPAALAGLKQMTDYI--IGAETVLNDPEEFFNLIETHDGQTLKLY 472
G +HVL + NSP AGL+ D+I I + D + +L++ + + +K+
Sbjct: 11 GGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLKANVEKPVKML 70
Query: 473 VYNIETDTCREVTITPNSTW 532
+Y+ +T RE ++TP++ W
Sbjct: 71 IYSSKTLELREASVTPSNLW 90
>sp|Q9H8Y8|GORS2_HUMAN Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55) (p59) (Golgi
phosphoprotein 6) (GOLPH6)
Length = 452
Score = 258 bits (658), Expect = 2e-68
Identities = 118/202 (58%), Positives = 152/202 (75%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK+N +KP+ + I+
Sbjct: 13 GTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLKANVEKPVKMLIY 72
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
+SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++ NSPAALAGL+
Sbjct: 73 SSKTLELRETSVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVESNSPAALAGLRPH 132
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
+DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITPNS W
Sbjct: 133 SDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGC 192
Query: 557 XXXXXXXHRIPSLPFSNISSIN 622
HRIP+ PF I+
Sbjct: 193 GIGYGYLHRIPTRPFEEGKKIS 214
Score = 45.4 bits (106), Expect = 2e-04
Identities = 23/80 (28%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = +2
Query: 299 GANENVWHVLDIDPNSPAALAGLKQMTDYI--IGAETVLNDPEEFFNLIETHDGQTLKLY 472
G +HVL + NSP AGL+ D+I I + D + +L++ + + +K+
Sbjct: 11 GGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLKANVEKPVKML 70
Query: 473 VYNIETDTCREVTITPNSTW 532
+Y+ +T RE ++TP++ W
Sbjct: 71 IYSSKTLELRETSVTPSNLW 90
>sp|Q9R064|GORS2_RAT Golgi reassembly stacking protein 2 (GRS2) (Golgi reassembly
stacking protein of 55 kDa) (GRASP55)
Length = 454
Score = 258 bits (658), Expect = 2e-68
Identities = 118/202 (58%), Positives = 152/202 (75%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EGYHVLRVQE SPGH+AGL+ FFDF+V++ ++L++DN+ LK+LLK+N +KP+ + I+
Sbjct: 13 GTEGYHVLRVQENSPGHRAGLEPFFDFIVSISGSRLNKDNDTLKDLLKANVEKPVKMLIY 72
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
+SKT R + PS WGGQGLLGVSIRFCSF+GANENVWHVL+++ NSPAALAGL+
Sbjct: 73 SSKTLELREASVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVESNSPAALAGLRPH 132
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
+DYIIGA+TV+N+ E+ F+LIETH+ + LKLYVYN +TD CREV ITPNS W
Sbjct: 133 SDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGC 192
Query: 557 XXXXXXXHRIPSLPFSNISSIN 622
HRIP+ PF I+
Sbjct: 193 GIGYGYLHRIPTRPFEEGKKIS 214
Score = 45.4 bits (106), Expect = 2e-04
Identities = 23/80 (28%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = +2
Query: 299 GANENVWHVLDIDPNSPAALAGLKQMTDYI--IGAETVLNDPEEFFNLIETHDGQTLKLY 472
G +HVL + NSP AGL+ D+I I + D + +L++ + + +K+
Sbjct: 11 GGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSISGSRLNKDNDTLKDLLKANVEKPVKML 70
Query: 473 VYNIETDTCREVTITPNSTW 532
+Y+ +T RE ++TP++ W
Sbjct: 71 IYSSKTLELREASVTPSNLW 90
>sp|O35254|GR65_RAT Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65)
Length = 451
Score = 236 bits (601), Expect = 8e-62
Identities = 105/196 (53%), Positives = 145/196 (73%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK+N +KP+ L +F
Sbjct: 12 GGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVF 71
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
N KT R VE+VPS WGGQGLLG S+RFCSF A+E+VWHVLD++P+SPAALAGL+
Sbjct: 72 NMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEPSSPAALAGLRPY 131
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TPN+ W
Sbjct: 132 TDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTPNAAWGGEGSLGC 191
Query: 557 XXXXXXXHRIPSLPFS 604
HRIP+ P S
Sbjct: 192 GIGYGYLHRIPTQPSS 207
>sp|Q91X51|GR65_MOUSE Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65)
Length = 446
Score = 234 bits (597), Expect = 2e-61
Identities = 105/196 (53%), Positives = 144/196 (73%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK+N +KP+ L +F
Sbjct: 12 GGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVF 71
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
N KT R VE+VPS WGGQGLLG S+RFCSF A+E+VWHVLD++P+SPAALAGL
Sbjct: 72 NMKTMKVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEPSSPAALAGLCPY 131
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
TDYI+G++ +L + E+FF LIE+H+G+ LKL VYN E+D+CREVT+TPN+ W
Sbjct: 132 TDYIVGSDQILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTPNAAWGGEGSLGC 191
Query: 557 XXXXXXXHRIPSLPFS 604
HRIP+ P S
Sbjct: 192 GIGYGYLHRIPTQPSS 207
>sp|Q9BQQ3|GR65_HUMAN Golgi reassembly stacking protein 1 (Golgi reassembly stacking
protein of 65 kDa) (GRASP65) (Golgi peripheral membrane
protein p65) (Golgi phosphoprotein 5) (GOLPH5)
Length = 440
Score = 232 bits (591), Expect = 1e-60
Identities = 102/194 (52%), Positives = 143/194 (73%)
Frame = +2
Query: 17 GNEGYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPINLTIF 196
G EG+H+ VQE SP +AGL+ +FDF++ +G ++L+++N+ LK LLK+N +KP+ L +F
Sbjct: 13 GAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVF 72
Query: 197 NSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALAGLKQM 376
N KT R VE+VPS WGGQGLLG S+RFCSF A+E VWHVLD++P+SPAALAGL+
Sbjct: 73 NMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPY 132
Query: 377 TDYIIGAETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXXXXXXXX 556
TDY++G++ +L + E+FF LIE+H+G+ LKL VYN ++D+CREVT+TPN+ W
Sbjct: 133 TDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTPNAAWGGEGSLGC 192
Query: 557 XXXXXXXHRIPSLP 598
HRIP+ P
Sbjct: 193 GIGYGYLHRIPTQP 206
>sp|Q10149|YAT2_SCHPO Hypothetical protein C1D4.02c in chromosome I
Length = 345
Score = 101 bits (252), Expect = 2e-21
Identities = 58/198 (29%), Positives = 104/198 (52%), Gaps = 3/198 (1%)
Frame = +2
Query: 8 HNHGNE--GYHVLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEVLKELLKSNKDKPI 181
H +E G+ VL+V+ S + A +++++DF+ A+ L+ D + LL+ + + +
Sbjct: 20 HRESDESCGFRVLKVENDSKAYNARIESYYDFITAVNGILLNGDPSMFMALLRDSSPE-V 78
Query: 182 NLTIFNSKTQSFRNVELVPSQDWGGQGLLGVSIRFCSFEGANENVWHVLDIDPNSPAALA 361
L +F+ K Q R V + + D +G+ +++ S A + +WH+L++ +SP A A
Sbjct: 79 TLEVFSLKGQITRKVNIKINSD----EKIGMVLQWASIAPAVDAIWHILNVIDDSPVARA 134
Query: 362 GLKQMTDYIIGA-ETVLNDPEEFFNLIETHDGQTLKLYVYNIETDTCREVTITPNSTWXX 538
L DYI+G E ++ + +LIE+H + L+LY+YN D+ R+VTI PN W
Sbjct: 135 SLVPYEDYIVGTPEGMMTGEKALSDLIESHLNRPLRLYIYNHYRDSTRQVTIVPNRHWGG 194
Query: 539 XXXXXXXXXXXXXHRIPS 592
HR+P+
Sbjct: 195 NGAIGCGVGHGVLHRLPA 212
>sp|P40555|PSD9_YEAST Probable 26S proteasome regulatory subunit p27 (Proteasome
non-ATPase subunit 2)
Length = 220
Score = 37.7 bits (86), Expect = 0.043
Identities = 21/85 (24%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
Frame = +2
Query: 35 VLRVQEGSPGHKAGLQAFFDFVVAMGDTKLSQDNEV--LKELLKSNKDKPINLTIFNSKT 208
+ V GSP KA ++ D ++++G+ + +++ ++ ++ N+D+P+ + + +
Sbjct: 137 ISEVVPGSPSDKADIKVD-DKLISIGNVHAANHSKLQNIQMVVMKNEDRPLPVLLL--RE 193
Query: 209 QSFRNVELVPSQDWGGQGLLGVSIR 283
L PS++W G+GLLG I+
Sbjct: 194 GQILKTSLTPSRNWNGRGLLGCRIQ 218
>sp|O00233|PSD9_HUMAN 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
regulatory subunit p27)
Length = 223
Score = 37.7 bits (86), Expect = 0.043
Identities = 26/81 (32%), Positives = 45/81 (55%), Gaps = 2/81 (2%)
Frame = +2
Query: 44 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 217
+ GSP AGLQ D +V G +T+ Q + +++ ++ KP+N+T+ +
Sbjct: 142 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSLHNIGSVVQHSEGKPLNVTVIRRGEK-- 198
Query: 218 RNVELVPSQDWGGQGLLGVSI 280
+ LVP++ W G+GLLG +I
Sbjct: 199 HQLRLVPTR-WAGKGLLGCNI 218
>sp|Q9CR00|PSD9_MOUSE 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome
regulatory subunit p27)
Length = 222
Score = 37.4 bits (85), Expect = 0.057
Identities = 25/81 (30%), Positives = 46/81 (56%), Gaps = 2/81 (2%)
Frame = +2
Query: 44 VQEGSPGHKAGLQAFFDFVVAMG--DTKLSQDNEVLKELLKSNKDKPINLTIFNSKTQSF 217
+ GSP AGLQ D +V G +T+ Q + + +++ ++ KP+N+T+ +
Sbjct: 141 ISPGSPASIAGLQVD-DEIVEFGSVNTQNFQSVQNVGTVVQHSEGKPLNVTVIRRGEK-- 197
Query: 218 RNVELVPSQDWGGQGLLGVSI 280
+ L+P++ W G+GLLG +I
Sbjct: 198 HQLRLIPTR-WAGKGLLGCNI 217
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,925,045
Number of Sequences: 369166
Number of extensions: 2053567
Number of successful extensions: 5934
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5910
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)