Planaria EST Database

DrC_00077

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00077
         (1028 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P22307|NLTP_HUMAN  Nonspecific lipid-transfer protein, mi...    66   2e-10
sp|P07857|NLTP_BOVIN  Nonspecific lipid-transfer protein (St...    65   4e-10
sp|P11915|NLTP_RAT  Nonspecific lipid-transfer protein, mito...    65   4e-10
sp|Q07598|NLTP_CHICK  Nonspecific lipid-transfer protein, mi...    64   7e-10
sp|Q9WYG0|Y325_THEMA  Hypothetical oxidoreductase TM0325           64   9e-10
sp|P32020|NLTP_MOUSE  Nonspecific lipid-transfer protein, mi...    63   1e-09
sp|O62742|NLTP_RABIT  Nonspecific lipid-transfer protein, mi...    63   1e-09
sp|P97852|DHB4_RAT  Peroxisomal multifunctional enzyme type ...    60   1e-08
sp|P55541|Y4LA_RHISN  Putative short-chain type dehydrogenas...    60   1e-08
sp|P51660|DHB4_MOUSE  Peroxisomal multifunctional enzyme typ...    59   2e-08

>sp|P22307|NLTP_HUMAN Nonspecific lipid-transfer protein, mitochondrial precursor
           (NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
           carrier protein X) (SCP-X) (SCPX)
          Length = 547

 Score = 65.9 bits (159), Expect = 2e-10
 Identities = 40/115 (34%), Positives = 66/115 (57%), Gaps = 6/115 (5%)
 Frame = +2

Query: 620 TDPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGK 781
           +D    +  F +I+  L   GE+ VK +G  FAF +    G +   W +D+K+G+GS   
Sbjct: 428 SDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV-- 485

Query: 782 LESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
           L +SD+ +  D  +T    +F+ + +G++ P  AF  G++KI GN  LAMKL++L
Sbjct: 486 LPNSDKKA--DCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNL 538

>sp|P07857|NLTP_BOVIN Nonspecific lipid-transfer protein (Sterol carrier protein 2)
           (SCP-2)
          Length = 121

 Score = 64.7 bits (156), Expect = 4e-10
 Identities = 39/116 (33%), Positives = 65/116 (56%), Gaps = 6/116 (5%)
 Frame = +2

Query: 617 NTDPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAG 778
           + D    +  F +I+  L   GE+ VK +G  FAF +    G +   W +D+K+G+GS  
Sbjct: 3   SVDGFKANLVFKEIEKKLEDEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV- 61

Query: 779 KLESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
            L +SD+ +  D  +T    + + + +G++ P  AF  G++KI GN  LAMKL++L
Sbjct: 62  -LPNSDKKA--DCTITMADSDLLALMTGKMNPQTAFFQGKLKINGNMGLAMKLQNL 114

>sp|P11915|NLTP_RAT Nonspecific lipid-transfer protein, mitochondrial precursor
           (NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
           carrier protein X) (SCP-X) (SCPX)
          Length = 547

 Score = 64.7 bits (156), Expect = 4e-10
 Identities = 41/114 (35%), Positives = 63/114 (55%), Gaps = 6/114 (5%)
 Frame = +2

Query: 623 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 784
           D    +  F +I+  L   GEE VK +G  FAF +    G +   W +D+K+G+GS   L
Sbjct: 429 DGFKANLIFKEIEKKLEEEGEEFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486

Query: 785 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
             SD+ +  D  +T    + + + +G++ P  AF  G++KI GN  LAMKL+SL
Sbjct: 487 PDSDKKA--DCTITMADSDLLALMTGKMNPQSAFFQGKLKIAGNMGLAMKLQSL 538

>sp|Q07598|NLTP_CHICK Nonspecific lipid-transfer protein, mitochondrial precursor
           (NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
           carrier protein X) (SCP-X) (SCPX)
          Length = 547

 Score = 63.9 bits (154), Expect = 7e-10
 Identities = 39/117 (33%), Positives = 63/117 (53%), Gaps = 3/117 (2%)
 Frame = +2

Query: 605 AAFGNTDPHSVSKTFSKIQGMLGEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSA 775
           AA      H V K   K     GE+ VK +G  FAF +    G +   W +D+K+G+GS 
Sbjct: 426 AAVDGFKSHLVFKEIEKKLQEEGEQFVKKIGGVFAFKIKDGPGGKEATWVVDVKNGKGSV 485

Query: 776 GKLESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
               +SD+ ++  + M  T  + + + +G++ P  AF  G++KI GN  +AMKL++L
Sbjct: 486 AV--NSDKKADCTITMADT--DLLALMTGKMNPQTAFFQGKLKISGNMGMAMKLQNL 538

>sp|Q9WYG0|Y325_THEMA Hypothetical oxidoreductase TM0325
          Length = 251

 Score = 63.5 bits (153), Expect = 9e-10
 Identities = 36/114 (31%), Positives = 58/114 (50%)
 Frame = +2

Query: 11  TIKVFGRIDILVNNASAISLTNTNDTMMKTYDLMNSINARGTYLCSKLCIPHIAKSGPGG 190
           T++ FGR+DILVNNA  +   N  +T  + +D   ++N +G +L SK  +  + K G GG
Sbjct: 74  TVETFGRLDILVNNAGIVPYGNIEETSEEDFDKTMAVNVKGPFLLSKYAVEQMKKQG-GG 132

Query: 191 HVVNISPPLNMSAKWFQNHVAYTMSKFGMSMCVLGMAEELKSKNIAVNALWPKT 352
            +VN+S    +          Y++SK  +      +A +     I VNA+ P T
Sbjct: 133 VIVNVSSEAGLIG--IPRRCVYSVSKAALLGLTRSLAVDYVDYGIRVNAVCPGT 184

>sp|P32020|NLTP_MOUSE Nonspecific lipid-transfer protein, mitochondrial precursor
           (NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
           carrier protein X) (SCP-X) (SCPX)
          Length = 547

 Score = 63.2 bits (152), Expect = 1e-09
 Identities = 39/114 (34%), Positives = 64/114 (56%), Gaps = 6/114 (5%)
 Frame = +2

Query: 623 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 784
           D    +  F +I+  L   GE+ VK +G  FAF +    G +   W +D+K+G+GS   L
Sbjct: 429 DGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486

Query: 785 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
            +SD+ +  D  +T    + + + +G++ P  AF  G++KI GN  LAMKL++L
Sbjct: 487 PNSDKKA--DCTITMADSDLLALMTGKMNPQSAFFQGKLKIAGNMGLAMKLQNL 538

>sp|O62742|NLTP_RABIT Nonspecific lipid-transfer protein, mitochondrial precursor (Sterol
           carrier protein 2) (SCP-2) (Sterol carrier protein X)
           (SCP-X) (SCPX)
          Length = 547

 Score = 63.2 bits (152), Expect = 1e-09
 Identities = 39/114 (34%), Positives = 64/114 (56%), Gaps = 6/114 (5%)
 Frame = +2

Query: 623 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 784
           D    +  F +I+  L   GE+ VK +G  FAF +    G +   W +D+K+G+GS   L
Sbjct: 429 DGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486

Query: 785 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 946
            +SD+ +  D  +T    + + + +G++ P  AF  G++KI GN  LAMKL++L
Sbjct: 487 PNSDKKA--DCTITIADSDLLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNL 538

>sp|P97852|DHB4_RAT Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional
           protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4)
           (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA
           dehydratase ; 3-hydroxyacyl-CoA dehydrogenase ]
          Length = 735

 Score = 60.1 bits (144), Expect = 1e-08
 Identities = 34/101 (33%), Positives = 55/101 (54%), Gaps = 2/101 (1%)
 Frame = +2

Query: 668 LGEEAVKSVGASFAFILT--GSEPGNWYLDLKSGEGSAGKLESSDQASNFDVQMTTTSEN 841
           +G E VK V A F + +T  G+    W +DLK+G G   +  +   A   D  +T + E+
Sbjct: 637 VGREVVKKVNAVFEWHITKNGNVAAKWTIDLKNGSGEVYQGPAKGSA---DTTITISDED 693

Query: 842 FVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIK 964
           F+++  G+L P  AF SG +K +GN  L+ KL+ ++    K
Sbjct: 694 FMEVVLGKLNPQNAFFSGRLKARGNIMLSQKLQMILKDYAK 734


 Score = 37.7 bits (86), Expect = 0.054
 Identities = 24/111 (21%), Positives = 53/111 (47%)
 Frame = +2

Query: 14  IKVFGRIDILVNNASAISLTNTNDTMMKTYDLMNSINARGTYLCSKLCIPHIAKSGPGGH 193
           +  FGRID++VNNA  +   + +    + +D++  ++ RG++  ++    H+ K    G 
Sbjct: 87  LDTFGRIDVVVNNAGILRDRSFSRISDEDWDIIQRVHLRGSFQVTRAAWDHMKKQN-YGR 145

Query: 194 VVNISPPLNMSAKWFQNHVAYTMSKFGMSMCVLGMAEELKSKNIAVNALWP 346
           ++  +    +   + Q +  Y+ +K G+      +  E +  NI  N + P
Sbjct: 146 IIMTASASGIYGNFGQAN--YSAAKLGLLGLANTLVIEGRKNNIHCNTIAP 194

>sp|P55541|Y4LA_RHISN Putative short-chain type dehydrogenase/reductase Y4LA
          Length = 278

 Score = 59.7 bits (143), Expect = 1e-08
 Identities = 40/112 (35%), Positives = 58/112 (51%), Gaps = 4/112 (3%)
 Frame = +2

Query: 23  FGRIDILVNNASAISLTNTN----DTMMKTYDLMNSINARGTYLCSKLCIPHIAKSGPGG 190
           FG +D+LVNNASA+ LT  +    D  +  +D   + N RGT LC +  IP +   G GG
Sbjct: 81  FGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLRGTLLCCRQAIPRMIARG-GG 139

Query: 191 HVVNISPPLNMSAKWFQNHVAYTMSKFGMSMCVLGMAEELKSKNIAVNALWP 346
            +VN+S    +S    Q   +Y +SK  M+M    +A +     I  NA+ P
Sbjct: 140 AIVNMSSCQGLSGDTAQ--TSYAVSKAAMNMLSASLATQYGHAQIRCNAVAP 189

>sp|P51660|DHB4_MOUSE Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional
           protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4)
           (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA
           dehydratase ; 3-hydroxyacyl-CoA dehydrogenase ]
          Length = 735

 Score = 59.3 bits (142), Expect = 2e-08
 Identities = 34/101 (33%), Positives = 54/101 (53%), Gaps = 2/101 (1%)
 Frame = +2

Query: 668 LGEEAVKSVGASFAFILT--GSEPGNWYLDLKSGEGSAGKLESSDQASNFDVQMTTTSEN 841
           +G E VK   A F + +T  G+    W +DLKSG G   +  +   A   DV +  + E+
Sbjct: 637 VGREVVKKANAVFEWHITKGGTVAAKWTIDLKSGSGEVYQGPAKGSA---DVTIIISDED 693

Query: 842 FVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIK 964
           F+++  G+L P  AF SG +K +GN  L+ KL+ ++    K
Sbjct: 694 FMEVVFGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAK 734


 Score = 39.3 bits (90), Expect = 0.018
 Identities = 26/111 (23%), Positives = 55/111 (49%)
 Frame = +2

Query: 14  IKVFGRIDILVNNASAISLTNTNDTMMKTYDLMNSINARGTYLCSKLCIPHIAKSGPGGH 193
           +  FGRID++VNNA  +   + +    + +D+++ ++ RG++  ++    H+ K    G 
Sbjct: 87  LDTFGRIDVVVNNAGILRDRSFSRISDEDWDIIHRVHLRGSFQVTRAAWDHMKKQN-YGR 145

Query: 194 VVNISPPLNMSAKWFQNHVAYTMSKFGMSMCVLGMAEELKSKNIAVNALWP 346
           ++  S    +   + Q +  Y+ +K G+      +A E +  NI  N + P
Sbjct: 146 ILMTSSASGIYGNFGQAN--YSAAKLGILGLCNTLAIEGRKNNIHCNTIAP 194

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 122,595,690
Number of Sequences: 369166
Number of extensions: 2655563
Number of successful extensions: 6849
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6552
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6813
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 11053210245
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00077

  1. Dr_sW_002_P13
  2. Dr_sW_019_D08
  3. Dr_sW_009_G12
  4. Dr_sW_026_H15