Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_H15
(464 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P22307|NLTP_HUMAN Nonspecific lipid-transfer protein, mi... 66 4e-11
sp|P07857|NLTP_BOVIN Nonspecific lipid-transfer protein (St... 65 1e-10
sp|P11915|NLTP_RAT Nonspecific lipid-transfer protein, mito... 65 1e-10
sp|Q07598|NLTP_CHICK Nonspecific lipid-transfer protein, mi... 64 2e-10
sp|P32020|NLTP_MOUSE Nonspecific lipid-transfer protein, mi... 63 3e-10
sp|O62742|NLTP_RABIT Nonspecific lipid-transfer protein, mi... 63 3e-10
sp|P97852|DHB4_RAT Peroxisomal multifunctional enzyme type ... 60 2e-09
sp|P51660|DHB4_MOUSE Peroxisomal multifunctional enzyme typ... 59 4e-09
sp|P51659|DHB4_HUMAN Peroxisomal multifunctional enzyme typ... 59 5e-09
sp|Q23655|NLT1_CAEEL Nonspecific lipid-transfer protein-like 58 1e-08
>sp|P22307|NLTP_HUMAN Nonspecific lipid-transfer protein, mitochondrial precursor
(NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
carrier protein X) (SCP-X) (SCPX)
Length = 547
Score = 65.9 bits (159), Expect = 4e-11
Identities = 40/115 (34%), Positives = 66/115 (57%), Gaps = 6/115 (5%)
Frame = +2
Query: 56 TDPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGK 217
+D + F +I+ L GE+ VK +G FAF + G + W +D+K+G+GS
Sbjct: 428 SDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV-- 485
Query: 218 LESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
L +SD+ + D +T +F+ + +G++ P AF G++KI GN LAMKL++L
Sbjct: 486 LPNSDKKA--DCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNL 538
>sp|P07857|NLTP_BOVIN Nonspecific lipid-transfer protein (Sterol carrier protein 2)
(SCP-2)
Length = 121
Score = 64.7 bits (156), Expect = 1e-10
Identities = 39/116 (33%), Positives = 65/116 (56%), Gaps = 6/116 (5%)
Frame = +2
Query: 53 NTDPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAG 214
+ D + F +I+ L GE+ VK +G FAF + G + W +D+K+G+GS
Sbjct: 3 SVDGFKANLVFKEIEKKLEDEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV- 61
Query: 215 KLESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
L +SD+ + D +T + + + +G++ P AF G++KI GN LAMKL++L
Sbjct: 62 -LPNSDKKA--DCTITMADSDLLALMTGKMNPQTAFFQGKLKINGNMGLAMKLQNL 114
>sp|P11915|NLTP_RAT Nonspecific lipid-transfer protein, mitochondrial precursor
(NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
carrier protein X) (SCP-X) (SCPX)
Length = 547
Score = 64.7 bits (156), Expect = 1e-10
Identities = 41/114 (35%), Positives = 63/114 (55%), Gaps = 6/114 (5%)
Frame = +2
Query: 59 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 220
D + F +I+ L GEE VK +G FAF + G + W +D+K+G+GS L
Sbjct: 429 DGFKANLIFKEIEKKLEEEGEEFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486
Query: 221 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
SD+ + D +T + + + +G++ P AF G++KI GN LAMKL+SL
Sbjct: 487 PDSDKKA--DCTITMADSDLLALMTGKMNPQSAFFQGKLKIAGNMGLAMKLQSL 538
>sp|Q07598|NLTP_CHICK Nonspecific lipid-transfer protein, mitochondrial precursor
(NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
carrier protein X) (SCP-X) (SCPX)
Length = 547
Score = 63.9 bits (154), Expect = 2e-10
Identities = 39/117 (33%), Positives = 63/117 (53%), Gaps = 3/117 (2%)
Frame = +2
Query: 41 AAFGNTDPHSVSKTFSKIQGMLGEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSA 211
AA H V K K GE+ VK +G FAF + G + W +D+K+G+GS
Sbjct: 426 AAVDGFKSHLVFKEIEKKLQEEGEQFVKKIGGVFAFKIKDGPGGKEATWVVDVKNGKGSV 485
Query: 212 GKLESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
+SD+ ++ + M T + + + +G++ P AF G++KI GN +AMKL++L
Sbjct: 486 AV--NSDKKADCTITMADT--DLLALMTGKMNPQTAFFQGKLKISGNMGMAMKLQNL 538
>sp|P32020|NLTP_MOUSE Nonspecific lipid-transfer protein, mitochondrial precursor
(NSL-TP) (Sterol carrier protein 2) (SCP-2) (Sterol
carrier protein X) (SCP-X) (SCPX)
Length = 547
Score = 63.2 bits (152), Expect = 3e-10
Identities = 39/114 (34%), Positives = 64/114 (56%), Gaps = 6/114 (5%)
Frame = +2
Query: 59 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 220
D + F +I+ L GE+ VK +G FAF + G + W +D+K+G+GS L
Sbjct: 429 DGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486
Query: 221 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
+SD+ + D +T + + + +G++ P AF G++KI GN LAMKL++L
Sbjct: 487 PNSDKKA--DCTITMADSDLLALMTGKMNPQSAFFQGKLKIAGNMGLAMKLQNL 538
>sp|O62742|NLTP_RABIT Nonspecific lipid-transfer protein, mitochondrial precursor (Sterol
carrier protein 2) (SCP-2) (Sterol carrier protein X)
(SCP-X) (SCPX)
Length = 547
Score = 63.2 bits (152), Expect = 3e-10
Identities = 39/114 (34%), Positives = 64/114 (56%), Gaps = 6/114 (5%)
Frame = +2
Query: 59 DPHSVSKTFSKIQGML---GEEAVKSVGASFAFILT---GSEPGNWYLDLKSGEGSAGKL 220
D + F +I+ L GE+ VK +G FAF + G + W +D+K+G+GS L
Sbjct: 429 DGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSV--L 486
Query: 221 ESSDQASNFDVQMTTTSENFVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESL 382
+SD+ + D +T + + + +G++ P AF G++KI GN LAMKL++L
Sbjct: 487 PNSDKKA--DCTITIADSDLLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNL 538
>sp|P97852|DHB4_RAT Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional
protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4)
(17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA
dehydratase ; 3-hydroxyacyl-CoA dehydrogenase ]
Length = 735
Score = 60.1 bits (144), Expect = 2e-09
Identities = 34/101 (33%), Positives = 55/101 (54%), Gaps = 2/101 (1%)
Frame = +2
Query: 104 LGEEAVKSVGASFAFILT--GSEPGNWYLDLKSGEGSAGKLESSDQASNFDVQMTTTSEN 277
+G E VK V A F + +T G+ W +DLK+G G + + A D +T + E+
Sbjct: 637 VGREVVKKVNAVFEWHITKNGNVAAKWTIDLKNGSGEVYQGPAKGSA---DTTITISDED 693
Query: 278 FVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIK 400
F+++ G+L P AF SG +K +GN L+ KL+ ++ K
Sbjct: 694 FMEVVLGKLNPQNAFFSGRLKARGNIMLSQKLQMILKDYAK 734
>sp|P51660|DHB4_MOUSE Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional
protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4)
(17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA
dehydratase ; 3-hydroxyacyl-CoA dehydrogenase ]
Length = 735
Score = 59.3 bits (142), Expect = 4e-09
Identities = 34/101 (33%), Positives = 54/101 (53%), Gaps = 2/101 (1%)
Frame = +2
Query: 104 LGEEAVKSVGASFAFILT--GSEPGNWYLDLKSGEGSAGKLESSDQASNFDVQMTTTSEN 277
+G E VK A F + +T G+ W +DLKSG G + + A DV + + E+
Sbjct: 637 VGREVVKKANAVFEWHITKGGTVAAKWTIDLKSGSGEVYQGPAKGSA---DVTIIISDED 693
Query: 278 FVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIK 400
F+++ G+L P AF SG +K +GN L+ KL+ ++ K
Sbjct: 694 FMEVVFGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAK 734
>sp|P51659|DHB4_HUMAN Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional
protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4)
(17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA
dehydratase ; 3-hydroxyacyl-CoA dehydrogenase ]
Length = 736
Score = 58.9 bits (141), Expect = 5e-09
Identities = 34/101 (33%), Positives = 54/101 (53%), Gaps = 2/101 (1%)
Frame = +2
Query: 104 LGEEAVKSVGASFAFILT--GSEPGNWYLDLKSGEGSAGKLESSDQASNFDVQMTTTSEN 277
+G E VK V A F + +T G+ W +DLKSG +GK+ D + + E+
Sbjct: 638 IGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSG---SGKVYQGPAKGAADTTIILSDED 694
Query: 278 FVKMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIK 400
F+++ G+L P AF SG +K +GN L+ KL+ ++ K
Sbjct: 695 FMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAK 735
>sp|Q23655|NLT1_CAEEL Nonspecific lipid-transfer protein-like
Length = 118
Score = 57.8 bits (138), Expect = 1e-08
Identities = 31/100 (31%), Positives = 53/100 (53%), Gaps = 2/100 (2%)
Frame = +2
Query: 110 EEAVKSVGASFAFILTGSEPGN--WYLDLKSGEGSAGKLESSDQASNFDVQMTTTSENFV 283
+E VK VG SF + G++ W +D KS G D + ++++ +F+
Sbjct: 20 KEMVKKVGTSFRMTIAGADGKTKVWTIDAKSDTPYVG----DDSSRPVEIEINIKDSDFI 75
Query: 284 KMFSGELVPSVAFMSGEMKIKGNAFLAMKLESLMSKSIKK 403
+ +G++ P AFM G+MK+KGN AMKL +++ + K
Sbjct: 76 AIAAGKMKPDQAFMQGKMKLKGNIAKAMKLRTILDPKMLK 115
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,707,804
Number of Sequences: 369166
Number of extensions: 916553
Number of successful extensions: 2531
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2518
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2633927485
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)