Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_00072 (564 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|O43708|MAAI_HUMAN Maleylacetoacetate isomerase (MAAI) (G... 149 4e-36 sp|Q9WVL0|MAAI_MOUSE Maleylacetoacetate isomerase (MAAI) (G... 147 2e-35 sp|Q9VHD2|MAAI2_DROME Probable maleylacetoacetate isomerase... 135 9e-32 sp|Q9VHD3|MAAI1_DROME Probable maleylacetoacetate isomerase... 124 2e-28 sp|Q18938|MAAI_CAEEL Probable maleylacetoacetate isomerase ... 106 5e-23 sp|Q9ZVQ3|GSTZ1_ARATH Glutathione S-transferase zeta-class ... 96 5e-20 sp|Q9KSB2|MAAI_VIBCH Probable maleylacetoacetate isomerase ... 96 5e-20 sp|Q9ZVQ4|GSTZ2_ARATH Probable glutathione S-transferase ze... 94 2e-19 sp|O43123|MAAI_EMENI Maleylacetoacetate isomerase (MAAI) 92 7e-19 sp|O04437|GSTZ_WHEAT Glutathione S-transferase (GST class-z... 88 2e-17
>sp|O43708|MAAI_HUMAN Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta 1) (GSTZ1-1) Length = 216 Score = 149 bits (377), Expect = 4e-36 Identities = 82/165 (49%), Positives = 106/165 (64%), Gaps = 1/165 (0%) Frame = +2 Query: 2 AINPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGI 181 A+NPM QVPT+ IDG+T+ Q + P+LLP + +RA R I+++I GI Sbjct: 52 ALNPMKQVPTLKIDGITIHQSLAIIEYLEETRPT-PRLLPQDPKKRASVRMISDLIAGGI 110 Query: 182 QPLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVPQV 358 QPLQNL LK V + WA +AI GF A+E IL TA YCVGDE+++AD+CLVPQV Sbjct: 111 QPLQNLSVLKQVGEEMQLTWAQNAITCGFNALEQILQSTAGIYCVGDEVTMADLCLVPQV 170 Query: 359 YNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCPEDQR 493 NA RFKV++ +P IS IN LL+ EAFQ + P QPD P + R Sbjct: 171 ANAERFKVDLTPYPTISSINKRLLVLEAFQVSHPCRQPDTPTELR 215
>sp|Q9WVL0|MAAI_MOUSE Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta 1) (GSTZ1-1) Length = 216 Score = 147 bits (371), Expect = 2e-35 Identities = 81/164 (49%), Positives = 100/164 (60%), Gaps = 1/164 (0%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 +NPM QVP + IDG+T+ Q P+LLP + +RA R I+++I SGIQ Sbjct: 53 LNPMKQVPALKIDGITIVQSLAIMEYLEETRPI-PRLLPQDPQKRAIVRMISDLIASGIQ 111 Query: 185 PLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVPQVY 361 PLQNL LK V Q WA I GF A+E IL TA YCVGDE+S+AD+CLVPQV Sbjct: 112 PLQNLSVLKQVGQENQMQWAQKVITSGFNALEKILQSTAGKYCVGDEVSMADVCLVPQVA 171 Query: 362 NANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCPEDQR 493 NA RFKV++ +P IS IN LL E FQ + P QPD P + R Sbjct: 172 NAERFKVDLSPYPTISHINKELLALEVFQVSHPRRQPDTPAELR 215
>sp|Q9VHD2|MAAI2_DROME Probable maleylacetoacetate isomerase 2 (MAAI 2) Length = 227 Score = 135 bits (339), Expect = 9e-32 Identities = 77/162 (47%), Positives = 98/162 (60%), Gaps = 1/162 (0%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 +NPM QVP + IDG TL + P LLP + +RA+ R I EII SGIQ Sbjct: 63 VNPMEQVPALQIDGHTLIESVAIMHYLEETRPQRP-LLPQDVHKRAKVREIVEIICSGIQ 121 Query: 185 PLQNLKTL-KVVQGTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVPQVY 361 PLQNL L V + WA I GF+A+E L +A YCVGDEIS+AD CLVPQV+ Sbjct: 122 PLQNLIVLIHVGEEKKKEWAQHWITRGFRAVEKALSTSAGKYCVGDEISMADCCLVPQVF 181 Query: 362 NANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCPED 487 NA RF V++ +P+I +I+ L N AF+ A P+ QPDCP + Sbjct: 182 NARRFHVDLRPYPIILRIDRELESNPAFRAAHPSNQPDCPPE 223
>sp|Q9VHD3|MAAI1_DROME Probable maleylacetoacetate isomerase 1 (MAAI 1) Length = 246 Score = 124 bits (310), Expect = 2e-28 Identities = 67/162 (41%), Positives = 96/162 (59%), Gaps = 1/162 (0%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 +NPM +VP++ IDG TL P LLP + ++RA+ R I E+I SGIQ Sbjct: 82 VNPMQKVPSLKIDGHTLCDSVAIIHYLEETRPQ-PALLPQDPVKRAKIREIVELICSGIQ 140 Query: 185 PLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVPQVY 361 PLQN+ L + + WA I GF+ +E +L +A +CVGDE+S+ADICLVPQV Sbjct: 141 PLQNVSVLDHIGKDQSLQWAQHWISRGFQGLEKVLSHSAGKFCVGDELSMADICLVPQVR 200 Query: 362 NANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCPED 487 NA R+K ++ +P I ++N L + F+ P+ QPDCP + Sbjct: 201 NARRYKADLTPYPTIVRLNQELQELDVFKATHPSTQPDCPPE 242
>sp|Q18938|MAAI_CAEEL Probable maleylacetoacetate isomerase (MAAI) Length = 214 Score = 106 bits (264), Expect = 5e-23 Identities = 59/161 (36%), Positives = 94/161 (58%), Gaps = 4/161 (2%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 INP +VPT +DG +T+ P LLP + ++RA AR I+ ++ SGIQ Sbjct: 50 INPAAKVPTFVVDGQVITESLAIIEYLEETHPDVP-LLPKDPIKRAHARAISLLVASGIQ 108 Query: 185 PLQNLKTLKVVQ----GTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVP 352 PL NLK L+++ G +A + +G A+E +L + + Y VGD+++IAD+ + P Sbjct: 109 PLHNLKVLQLLNKKEAGFGGQFAKQFVVEGLTALEILLKQHSGKYAVGDDVTIADLSIPP 168 Query: 353 QVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPD 475 +Y+ANRF +++ +P +++IN TL AF A P+ QPD Sbjct: 169 LIYSANRFNLDLSPYPTVNRINETLADIPAFIAAHPDNQPD 209
>sp|Q9ZVQ3|GSTZ1_ARATH Glutathione S-transferase zeta-class 1 (AtGSTZ1) (Maleylacetone isomerase) (MAI) Length = 221 Score = 96.3 bits (238), Expect = 5e-20 Identities = 59/165 (35%), Positives = 85/165 (51%), Gaps = 6/165 (3%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 INPM VP + +DG + P LLP + +RA I+ SGIQ Sbjct: 54 INPMGTVPAL-VDGDVVINDSFAIIMYLDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQ 112 Query: 185 PLQNLKTLKVVQGTY-----PNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLV 349 P QNL ++ ++ W ++AI GF A+E +L+ A + GDEI +AD+ L Sbjct: 113 PHQNLAVIRYIEEKINVEEKTAWVNNAITKGFTALEKLLVNCAGKHATGDEIYLADLFLA 172 Query: 350 PQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCP 481 PQ++ A NRF++NME +P ++K + AFQ A P QPD P Sbjct: 173 PQIHGAINRFQINMEPYPTLAKCYESYNELPAFQNALPEKQPDAP 217
>sp|Q9KSB2|MAAI_VIBCH Probable maleylacetoacetate isomerase (MAAI) Length = 215 Score = 96.3 bits (238), Expect = 5e-20 Identities = 56/164 (34%), Positives = 82/164 (50%), Gaps = 7/164 (4%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 +NP +P + IDG P+L+P +R Q + +A I + I Sbjct: 51 LNPSELIPVL-IDGELCLNQSLAIIEYLDETYPAPRLIPERGAERYQVKALALDIAADIH 109 Query: 185 PLQNLKTLK-------VVQGTYPNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADIC 343 P+ NL+ L+ V W I GF+ +E L TA YCVG+ +S+ D+C Sbjct: 110 PINNLRILQYLTAKLGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVGNRLSLVDVC 169 Query: 344 LVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPD 475 LVPQVYNA RF ++M ++P + +I + L AF +AAP QPD Sbjct: 170 LVPQVYNAERFDLDMSRYPTLQQIAARLRALPAFAQAAPENQPD 213
>sp|Q9ZVQ4|GSTZ2_ARATH Probable glutathione S-transferase zeta-class 2 Length = 223 Score = 94.0 bits (232), Expect = 2e-19 Identities = 58/165 (35%), Positives = 82/165 (49%), Gaps = 6/165 (3%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 INPM VP + +DG + P LLP + +RA I+ SGIQ Sbjct: 57 INPMGTVPAL-VDGDVVINDSFAIIMYLDDKYPEPPLLPSDYHKRAVNYQATSIVMSGIQ 115 Query: 185 PLQNLKTLKVVQGTY-----PNWAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLV 349 P QN+ + ++ W +AI GF A+E +L+ A Y GDE+ +AD+ L Sbjct: 116 PHQNMALFRYLEDKINAEEKTAWITNAITKGFTALEKLLVSCAGKYATGDEVYLADLFLA 175 Query: 350 PQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCP 481 PQ++ A NRF +NME FP +++ + AFQ A P QPD P Sbjct: 176 PQIHAAFNRFHINMEPFPTLARFYESYNELPAFQNAVPEKQPDTP 220
>sp|O43123|MAAI_EMENI Maleylacetoacetate isomerase (MAAI) Length = 230 Score = 92.4 bits (228), Expect = 7e-19 Identities = 64/177 (36%), Positives = 88/177 (49%), Gaps = 13/177 (7%) Frame = +2 Query: 2 AINPMHQVPTIDIDGV-------TLTQXXXXXXXXXXXFTSGPK-LLP--GNTLQRAQAR 151 A+NP VPT+ I+ V T+TQ F P LLP N QRA R Sbjct: 53 AVNPSATVPTLIIEHVDRSQSPITITQSLAALEYLDEAFPDNPNPLLPPISNPQQRALVR 112 Query: 152 CIAEIINSGIQPLQNLKTLKVVQGTYPN---WAHDAIRDGFKAIEAILIKTAKTYCVGDE 322 +A II IQP+ NL+ L+ V + W+ D I GF A EAI +A + VGD Sbjct: 113 SLASIIACDIQPVTNLRILQRVAPFGVDRAAWSKDLIEAGFAAYEAIARDSAGVFSVGDT 172 Query: 323 ISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCPEDQR 493 I++AD+CL+P V+ A R VN+ Q+P I ++ L A ++ Q D P + R Sbjct: 173 ITMADVCLIPAVWGAERAGVNLGQYPTIKRVAEALEKENAVKEGHWRTQQDTPTEFR 229
>sp|O04437|GSTZ_WHEAT Glutathione S-transferase (GST class-zeta) Length = 213 Score = 87.8 bits (216), Expect = 2e-17 Identities = 52/165 (31%), Positives = 87/165 (52%), Gaps = 6/165 (3%) Frame = +2 Query: 5 INPMHQVPTIDIDGVTLTQXXXXXXXXXXXFTSGPKLLPGNTLQRAQARCIAEIINSGIQ 184 INP+ +P + L+ + P L+P + + IA I+ S IQ Sbjct: 47 INPIKYIPALVDGDFVLSDSLAIMLYLEDKYPQHP-LVPKDIKTKGLDLQIANIVCSSIQ 105 Query: 185 PLQNLKTLKVVQGTY-PNWAHDAIR----DGFKAIEAILIKTAKTYCVGDEISIADICLV 349 PLQ + + +G P+ + + ++ GF+AIE +L YCVGDE+ + D+CL Sbjct: 106 PLQGYGVIGLHEGRLSPDESLEVVQRYIDKGFRAIEKLLDGCDSKYCVGDEVHLGDVCLA 165 Query: 350 PQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCP 481 PQ++ A NRF+++M ++P++S+++ + AFQ A P QPD P Sbjct: 166 PQIHAAINRFQIDMTKYPILSRLHDAYMKIPAFQAALPQNQPDAP 210
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 58,667,289 Number of Sequences: 369166 Number of extensions: 1083726 Number of successful extensions: 2490 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 2423 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2454 length of database: 68,354,980 effective HSP length: 104 effective length of database: 49,142,540 effective search space used: 4078830820 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)