Planarian EST Database


Dr_sW_002_C07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_002_C07
         (515 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9WVL0|MAAI_MOUSE  Maleylacetoacetate isomerase (MAAI) (G...   132   4e-31
sp|O43708|MAAI_HUMAN  Maleylacetoacetate isomerase (MAAI) (G...   130   1e-30
sp|Q9VHD2|MAAI2_DROME  Probable maleylacetoacetate isomerase...   119   6e-27
sp|Q9VHD3|MAAI1_DROME  Probable maleylacetoacetate isomerase...   112   7e-25
sp|Q18938|MAAI_CAEEL  Probable maleylacetoacetate isomerase ...    98   1e-20
sp|Q9KSB2|MAAI_VIBCH  Probable maleylacetoacetate isomerase ...    92   1e-18
sp|Q9ZVQ3|GSTZ1_ARATH  Glutathione S-transferase zeta-class ...    91   2e-18
sp|Q9ZVQ4|GSTZ2_ARATH  Probable glutathione S-transferase ze...    89   8e-18
sp|O04437|GSTZ_WHEAT  Glutathione S-transferase (GST class-z...    85   1e-16
sp|O43123|MAAI_EMENI  Maleylacetoacetate isomerase (MAAI)          84   2e-16
>sp|Q9WVL0|MAAI_MOUSE Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta
           1) (GSTZ1-1)
          Length = 216

 Score =  132 bits (333), Expect = 4e-31
 Identities = 70/130 (53%), Positives = 85/130 (65%), Gaps = 1/130 (0%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAI 234
           P+LLP +  +RA  R I+++I SGIQPLQNL  LK V Q     WA   I  GF A+E I
Sbjct: 86  PRLLPQDPQKRAIVRMISDLIASGIQPLQNLSVLKQVGQENQMQWAQKVITSGFNALEKI 145

Query: 235 LIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPN 414
           L  TA  YCVGDE+S+AD+CLVPQV NA RFKV++  +P IS IN  LL  E FQ + P 
Sbjct: 146 LQSTAGKYCVGDEVSMADVCLVPQVANAERFKVDLSPYPTISHINKELLALEVFQVSHPR 205

Query: 415 AQPDCPEDQR 444
            QPD P + R
Sbjct: 206 RQPDTPAELR 215
>sp|O43708|MAAI_HUMAN Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta
           1) (GSTZ1-1)
          Length = 216

 Score =  130 bits (328), Expect = 1e-30
 Identities = 69/130 (53%), Positives = 88/130 (67%), Gaps = 1/130 (0%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAI 234
           P+LLP +  +RA  R I+++I  GIQPLQNL  LK V +     WA +AI  GF A+E I
Sbjct: 86  PRLLPQDPKKRASVRMISDLIAGGIQPLQNLSVLKQVGEEMQLTWAQNAITCGFNALEQI 145

Query: 235 LIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPN 414
           L  TA  YCVGDE+++AD+CLVPQV NA RFKV++  +P IS IN  LL+ EAFQ + P 
Sbjct: 146 LQSTAGIYCVGDEVTMADLCLVPQVANAERFKVDLTPYPTISSINKRLLVLEAFQVSHPC 205

Query: 415 AQPDCPEDQR 444
            QPD P + R
Sbjct: 206 RQPDTPTELR 215
>sp|Q9VHD2|MAAI2_DROME Probable maleylacetoacetate isomerase 2 (MAAI 2)
          Length = 227

 Score =  119 bits (297), Expect = 6e-27
 Identities = 65/126 (51%), Positives = 82/126 (65%), Gaps = 1/126 (0%)
 Frame = +1

Query: 64  LLPGNTLQRAHARCIAEIINSGIQPLQNLKTL-KVVQGTYPNWAHDAIRDGFKAIEAILI 240
           LLP +  +RA  R I EII SGIQPLQNL  L  V +     WA   I  GF+A+E  L 
Sbjct: 98  LLPQDVHKRAKVREIVEIICSGIQPLQNLIVLIHVGEEKKKEWAQHWITRGFRAVEKALS 157

Query: 241 KTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQ 420
            +A  YCVGDEIS+AD CLVPQV+NA RF V++  +P+I +I+  L  N AF+ A P+ Q
Sbjct: 158 TSAGKYCVGDEISMADCCLVPQVFNARRFHVDLRPYPIILRIDRELESNPAFRAAHPSNQ 217

Query: 421 PDCPED 438
           PDCP +
Sbjct: 218 PDCPPE 223
>sp|Q9VHD3|MAAI1_DROME Probable maleylacetoacetate isomerase 1 (MAAI 1)
          Length = 246

 Score =  112 bits (279), Expect = 7e-25
 Identities = 57/128 (44%), Positives = 81/128 (63%), Gaps = 1/128 (0%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVV-QGTYPNWAHDAIRDGFKAIEAI 234
           P LLP + ++RA  R I E+I SGIQPLQN+  L  + +     WA   I  GF+ +E +
Sbjct: 115 PALLPQDPVKRAKIREIVELICSGIQPLQNVSVLDHIGKDQSLQWAQHWISRGFQGLEKV 174

Query: 235 LIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAPN 414
           L  +A  +CVGDE+S+ADICLVPQV NA R+K ++  +P I ++N  L   + F+   P+
Sbjct: 175 LSHSAGKFCVGDELSMADICLVPQVRNARRYKADLTPYPTIVRLNQELQELDVFKATHPS 234

Query: 415 AQPDCPED 438
            QPDCP +
Sbjct: 235 TQPDCPPE 242
>sp|Q18938|MAAI_CAEEL Probable maleylacetoacetate isomerase (MAAI)
          Length = 214

 Score = 98.2 bits (243), Expect = 1e-20
 Identities = 50/125 (40%), Positives = 81/125 (64%), Gaps = 4/125 (3%)
 Frame = +1

Query: 64  LLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVVQ----GTYPNWAHDAIRDGFKAIEA 231
           LLP + ++RAHAR I+ ++ SGIQPL NLK L+++     G    +A   + +G  A+E 
Sbjct: 85  LLPKDPIKRAHARAISLLVASGIQPLHNLKVLQLLNKKEAGFGGQFAKQFVVEGLTALEI 144

Query: 232 ILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQKAAP 411
           +L + +  Y VGD+++IAD+ + P +Y+ANRF +++  +P +++IN TL    AF  A P
Sbjct: 145 LLKQHSGKYAVGDDVTIADLSIPPLIYSANRFNLDLSPYPTVNRINETLADIPAFIAAHP 204

Query: 412 NAQPD 426
           + QPD
Sbjct: 205 DNQPD 209
>sp|Q9KSB2|MAAI_VIBCH Probable maleylacetoacetate isomerase (MAAI)
          Length = 215

 Score = 91.7 bits (226), Expect = 1e-18
 Identities = 49/130 (37%), Positives = 72/130 (55%), Gaps = 7/130 (5%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLK-------VVQGTYPNWAHDAIRDGF 216
           P+L+P    +R   + +A  I + I P+ NL+ L+       V       W    I  GF
Sbjct: 84  PRLIPERGAERYQVKALALDIAADIHPINNLRILQYLTAKLGVADEEKNRWYRHWIDKGF 143

Query: 217 KAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAF 396
           + +E  L  TA  YCVG+ +S+ D+CLVPQVYNA RF ++M ++P + +I + L    AF
Sbjct: 144 QGLEEKLRHTAGEYCVGNRLSLVDVCLVPQVYNAERFDLDMSRYPTLQQIAARLRALPAF 203

Query: 397 QKAAPNAQPD 426
            +AAP  QPD
Sbjct: 204 AQAAPENQPD 213
>sp|Q9ZVQ3|GSTZ1_ARATH Glutathione S-transferase zeta-class 1 (AtGSTZ1) (Maleylacetone
           isomerase) (MAI)
          Length = 221

 Score = 90.9 bits (224), Expect = 2e-18
 Identities = 51/131 (38%), Positives = 74/131 (56%), Gaps = 6/131 (4%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVVQGTY-----PNWAHDAIRDGFKA 222
           P LLP +  +RA       I+ SGIQP QNL  ++ ++          W ++AI  GF A
Sbjct: 87  PPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNAITKGFTA 146

Query: 223 IEAILIKTAKTYCVGDEISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQ 399
           +E +L+  A  +  GDEI +AD+ L PQ++ A NRF++NME +P ++K   +     AFQ
Sbjct: 147 LEKLLVNCAGKHATGDEIYLADLFLAPQIHGAINRFQINMEPYPTLAKCYESYNELPAFQ 206

Query: 400 KAAPNAQPDCP 432
            A P  QPD P
Sbjct: 207 NALPEKQPDAP 217
>sp|Q9ZVQ4|GSTZ2_ARATH Probable glutathione S-transferase zeta-class 2
          Length = 223

 Score = 88.6 bits (218), Expect = 8e-18
 Identities = 50/131 (38%), Positives = 71/131 (54%), Gaps = 6/131 (4%)
 Frame = +1

Query: 58  PKLLPGNTLQRAHARCIAEIINSGIQPLQNLKTLKVVQGTY-----PNWAHDAIRDGFKA 222
           P LLP +  +RA       I+ SGIQP QN+   + ++          W  +AI  GF A
Sbjct: 90  PPLLPSDYHKRAVNYQATSIVMSGIQPHQNMALFRYLEDKINAEEKTAWITNAITKGFTA 149

Query: 223 IEAILIKTAKTYCVGDEISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQ 399
           +E +L+  A  Y  GDE+ +AD+ L PQ++ A NRF +NME FP +++   +     AFQ
Sbjct: 150 LEKLLVSCAGKYATGDEVYLADLFLAPQIHAAFNRFHINMEPFPTLARFYESYNELPAFQ 209

Query: 400 KAAPNAQPDCP 432
            A P  QPD P
Sbjct: 210 NAVPEKQPDTP 220
>sp|O04437|GSTZ_WHEAT Glutathione S-transferase (GST class-zeta)
          Length = 213

 Score = 84.7 bits (208), Expect = 1e-16
 Identities = 44/115 (38%), Positives = 71/115 (61%), Gaps = 6/115 (5%)
 Frame = +1

Query: 106 IAEIINSGIQPLQNLKTLKVVQGTY-PNWAHDAIR----DGFKAIEAILIKTAKTYCVGD 270
           IA I+ S IQPLQ    + + +G   P+ + + ++     GF+AIE +L      YCVGD
Sbjct: 96  IANIVCSSIQPLQGYGVIGLHEGRLSPDESLEVVQRYIDKGFRAIEKLLDGCDSKYCVGD 155

Query: 271 EISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEAFQKAAPNAQPDCP 432
           E+ + D+CL PQ++ A NRF+++M ++P++S+++   +   AFQ A P  QPD P
Sbjct: 156 EVHLGDVCLAPQIHAAINRFQIDMTKYPILSRLHDAYMKIPAFQAALPQNQPDAP 210
>sp|O43123|MAAI_EMENI Maleylacetoacetate isomerase (MAAI)
          Length = 230

 Score = 84.0 bits (206), Expect = 2e-16
 Identities = 56/154 (36%), Positives = 77/154 (50%), Gaps = 6/154 (3%)
 Frame = +1

Query: 1   TLTQXXXXXXXXXXXFTSGPK-LLP--GNTLQRAHARCIAEIINSGIQPLQNLKTLKVVQ 171
           T+TQ           F   P  LLP   N  QRA  R +A II   IQP+ NL+ L+ V 
Sbjct: 76  TITQSLAALEYLDEAFPDNPNPLLPPISNPQQRALVRSLASIIACDIQPVTNLRILQRVA 135

Query: 172 GTYPN---WAHDAIRDGFKAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNME 342
               +   W+ D I  GF A EAI   +A  + VGD I++AD+CL+P V+ A R  VN+ 
Sbjct: 136 PFGVDRAAWSKDLIEAGFAAYEAIARDSAGVFSVGDTITMADVCLIPAVWGAERAGVNLG 195

Query: 343 QFPLISKINSTLLLNEAFQKAAPNAQPDCPEDQR 444
           Q+P I ++   L    A ++     Q D P + R
Sbjct: 196 QYPTIKRVAEALEKENAVKEGHWRTQQDTPTEFR 229
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,807,743
Number of Sequences: 369166
Number of extensions: 986049
Number of successful extensions: 2386
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2371
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)