Planarian EST Database


Dr_sW_007_I09

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_007_I09
         (330 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9WVL0|MAAI_MOUSE  Maleylacetoacetate isomerase (MAAI) (G...    89   3e-18
sp|O43708|MAAI_HUMAN  Maleylacetoacetate isomerase (MAAI) (G...    89   4e-18
sp|Q9VHD2|MAAI2_DROME  Probable maleylacetoacetate isomerase...    84   1e-16
sp|Q9VHD3|MAAI1_DROME  Probable maleylacetoacetate isomerase...    76   2e-14
sp|Q9KSB2|MAAI_VIBCH  Probable maleylacetoacetate isomerase ...    71   7e-13
sp|O04437|GSTZ_WHEAT  Glutathione S-transferase (GST class-z...    70   1e-12
sp|Q9ZVQ4|GSTZ2_ARATH  Probable glutathione S-transferase ze...    65   6e-11
sp|Q18938|MAAI_CAEEL  Probable maleylacetoacetate isomerase ...    65   6e-11
sp|Q9ZVQ3|GSTZ1_ARATH  Glutathione S-transferase zeta-class ...    64   8e-11
sp|O43123|MAAI_EMENI  Maleylacetoacetate isomerase (MAAI)          55   4e-08
>sp|Q9WVL0|MAAI_MOUSE Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta
           1) (GSTZ1-1)
          Length = 216

 Score = 89.0 bits (219), Expect = 3e-18
 Identities = 43/76 (56%), Positives = 53/76 (69%)
 Frame = +2

Query: 32  SAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAF 211
           +A+E IL  TA  YCVGDE+S+AD+CLVPQV NA RFKV++  +P IS IN  LL  E F
Sbjct: 140 NALEKILQSTAGKYCVGDEVSMADVCLVPQVANAERFKVDLSPYPTISHINKELLALEVF 199

Query: 212 QKAAPNAQPDCPEDQR 259
           Q + P  QPD P + R
Sbjct: 200 QVSHPRRQPDTPAELR 215
>sp|O43708|MAAI_HUMAN Maleylacetoacetate isomerase (MAAI) (Glutathione S-transferase zeta
           1) (GSTZ1-1)
          Length = 216

 Score = 88.6 bits (218), Expect = 4e-18
 Identities = 43/76 (56%), Positives = 55/76 (72%)
 Frame = +2

Query: 32  SAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAF 211
           +A+E IL  TA  YCVGDE+++AD+CLVPQV NA RFKV++  +P IS IN  LL+ EAF
Sbjct: 140 NALEQILQSTAGIYCVGDEVTMADLCLVPQVANAERFKVDLTPYPTISSINKRLLVLEAF 199

Query: 212 QKAAPNAQPDCPEDQR 259
           Q + P  QPD P + R
Sbjct: 200 QVSHPCRQPDTPTELR 215
>sp|Q9VHD2|MAAI2_DROME Probable maleylacetoacetate isomerase 2 (MAAI 2)
          Length = 227

 Score = 84.0 bits (206), Expect = 1e-16
 Identities = 39/73 (53%), Positives = 52/73 (71%)
 Frame = +2

Query: 35  AIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQ 214
           A+E  L  +A  YCVGDEIS+AD CLVPQV+NA RF V++  +P+I +I+  L  N AF+
Sbjct: 151 AVEKALSTSAGKYCVGDEISMADCCLVPQVFNARRFHVDLRPYPIILRIDRELESNPAFR 210

Query: 215 KAAPNAQPDCPED 253
            A P+ QPDCP +
Sbjct: 211 AAHPSNQPDCPPE 223
>sp|Q9VHD3|MAAI1_DROME Probable maleylacetoacetate isomerase 1 (MAAI 1)
          Length = 246

 Score = 76.3 bits (186), Expect = 2e-14
 Identities = 33/72 (45%), Positives = 49/72 (68%)
 Frame = +2

Query: 38  IEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQK 217
           +E +L  +A  +CVGDE+S+ADICLVPQV NA R+K ++  +P I ++N  L   + F+ 
Sbjct: 171 LEKVLSHSAGKFCVGDELSMADICLVPQVRNARRYKADLTPYPTIVRLNQELQELDVFKA 230

Query: 218 AAPNAQPDCPED 253
             P+ QPDCP +
Sbjct: 231 THPSTQPDCPPE 242
>sp|Q9KSB2|MAAI_VIBCH Probable maleylacetoacetate isomerase (MAAI)
          Length = 215

 Score = 71.2 bits (173), Expect = 7e-13
 Identities = 33/68 (48%), Positives = 46/68 (67%)
 Frame = +2

Query: 38  IEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAFQK 217
           +E  L  TA  YCVG+ +S+ D+CLVPQVYNA RF ++M ++P + +I + L    AF +
Sbjct: 146 LEEKLRHTAGEYCVGNRLSLVDVCLVPQVYNAERFDLDMSRYPTLQQIAARLRALPAFAQ 205

Query: 218 AAPNAQPD 241
           AAP  QPD
Sbjct: 206 AAPENQPD 213
>sp|O04437|GSTZ_WHEAT Glutathione S-transferase (GST class-zeta)
          Length = 213

 Score = 70.5 bits (171), Expect = 1e-12
 Identities = 31/72 (43%), Positives = 48/72 (66%), Gaps = 1/72 (1%)
 Frame = +2

Query: 35  AIEAILIKTAKTYCVGDEISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEAF 211
           AIE +L      YCVGDE+ + D+CL PQ++ A NRF+++M ++P++S+++   +   AF
Sbjct: 139 AIEKLLDGCDSKYCVGDEVHLGDVCLAPQIHAAINRFQIDMTKYPILSRLHDAYMKIPAF 198

Query: 212 QKAAPNAQPDCP 247
           Q A P  QPD P
Sbjct: 199 QAALPQNQPDAP 210
>sp|Q9ZVQ4|GSTZ2_ARATH Probable glutathione S-transferase zeta-class 2
          Length = 223

 Score = 64.7 bits (156), Expect = 6e-11
 Identities = 31/73 (42%), Positives = 45/73 (61%), Gaps = 1/73 (1%)
 Frame = +2

Query: 32  SAIEAILIKTAKTYCVGDEISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEA 208
           +A+E +L+  A  Y  GDE+ +AD+ L PQ++ A NRF +NME FP +++   +     A
Sbjct: 148 TALEKLLVSCAGKYATGDEVYLADLFLAPQIHAAFNRFHINMEPFPTLARFYESYNELPA 207

Query: 209 FQKAAPNAQPDCP 247
           FQ A P  QPD P
Sbjct: 208 FQNAVPEKQPDTP 220
>sp|Q18938|MAAI_CAEEL Probable maleylacetoacetate isomerase (MAAI)
          Length = 214

 Score = 64.7 bits (156), Expect = 6e-11
 Identities = 32/80 (40%), Positives = 53/80 (66%), Gaps = 2/80 (2%)
 Frame = +2

Query: 8   FAVYFVK--ISAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKI 181
           FA  FV   ++A+E +L + +  Y VGD+++IAD+ + P +Y+ANRF +++  +P +++I
Sbjct: 130 FAKQFVVEGLTALEILLKQHSGKYAVGDDVTIADLSIPPLIYSANRFNLDLSPYPTVNRI 189

Query: 182 NSTLLLNEAFQKAAPNAQPD 241
           N TL    AF  A P+ QPD
Sbjct: 190 NETLADIPAFIAAHPDNQPD 209
>sp|Q9ZVQ3|GSTZ1_ARATH Glutathione S-transferase zeta-class 1 (AtGSTZ1) (Maleylacetone
           isomerase) (MAI)
          Length = 221

 Score = 64.3 bits (155), Expect = 8e-11
 Identities = 31/73 (42%), Positives = 46/73 (63%), Gaps = 1/73 (1%)
 Frame = +2

Query: 32  SAIEAILIKTAKTYCVGDEISIADICLVPQVYNA-NRFKVNMEQFPLISKINSTLLLNEA 208
           +A+E +L+  A  +  GDEI +AD+ L PQ++ A NRF++NME +P ++K   +     A
Sbjct: 145 TALEKLLVNCAGKHATGDEIYLADLFLAPQIHGAINRFQINMEPYPTLAKCYESYNELPA 204

Query: 209 FQKAAPNAQPDCP 247
           FQ A P  QPD P
Sbjct: 205 FQNALPEKQPDAP 217
>sp|O43123|MAAI_EMENI Maleylacetoacetate isomerase (MAAI)
          Length = 230

 Score = 55.5 bits (132), Expect = 4e-08
 Identities = 28/76 (36%), Positives = 43/76 (56%)
 Frame = +2

Query: 32  SAIEAILIKTAKTYCVGDEISIADICLVPQVYNANRFKVNMEQFPLISKINSTLLLNEAF 211
           +A EAI   +A  + VGD I++AD+CL+P V+ A R  VN+ Q+P I ++   L    A 
Sbjct: 154 AAYEAIARDSAGVFSVGDTITMADVCLIPAVWGAERAGVNLGQYPTIKRVAEALEKENAV 213

Query: 212 QKAAPNAQPDCPEDQR 259
           ++     Q D P + R
Sbjct: 214 KEGHWRTQQDTPTEFR 229
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,745,631
Number of Sequences: 369166
Number of extensions: 490288
Number of successful extensions: 1190
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1186
length of database: 68,354,980
effective HSP length: 78
effective length of database: 53,945,650
effective search space used: 1672315150
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)