Planaria EST Database

DrC_00039

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00039
         (659 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P62255|UB2G1_RAT  Ubiquitin-conjugating enzyme E2 G1 (Ubi...   268   9e-72
sp|P34477|UBC7_CAEEL  Probable ubiquitin-conjugating enzyme ...   255   6e-68
sp|Q9Y818|UBC15_SCHPO  Ubiquitin-conjugating enzyme E2 15 (U...   236   3e-62
sp|Q42541|UBCD_ARATH  Ubiquitin-conjugating enzyme E2 13 (Ub...   228   8e-60
sp|Q42540|UBC7_ARATH  Ubiquitin-conjugating enzyme E2 7 (Ubi...   226   5e-59
sp|P42747|UBC14_ARATH  Ubiquitin-conjugating enzyme E2 14 (U...   224   1e-58
sp|P25868|UBC7_WHEAT  Ubiquitin-conjugating enzyme E2 7 (Ubi...   200   2e-51
sp|P60605|UB2G2_MOUSE  Ubiquitin-conjugating enzyme E2 G2 (U...   165   9e-41
sp|Q29503|UBC3_RABIT  Ubiquitin-conjugating enzyme E2-32 kDa...   164   2e-40
sp|Q02159|UBC7_YEAST  Ubiquitin-conjugating enzyme E2-18 kDa...   163   4e-40

>sp|P62255|UB2G1_RAT Ubiquitin-conjugating enzyme E2 G1 (Ubiquitin-protein ligase G1)
           (Ubiquitin carrier protein G1) (E217K) (UBC7)
 sp|P62254|UB2G1_MOUSE Ubiquitin-conjugating enzyme E2 G1 (Ubiquitin-protein ligase G1)
           (Ubiquitin carrier protein G1) (E217K) (UBC7)
 sp|P62253|UB2G1_HUMAN Ubiquitin-conjugating enzyme E2 G1 (Ubiquitin-protein ligase G1)
           (Ubiquitin carrier protein G1) (E217K) (UBC7)
          Length = 170

 Score =  268 bits (685), Expect = 9e-72
 Identities = 125/165 (75%), Positives = 146/165 (88%), Gaps = 1/165 (0%)
 Frame = +3

Query: 33  TNSASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDF 212
           T   S LLL++QLAELNKN  EGFSAGL+DD ++++W+VLIIGPPDTLYEGG FKA L F
Sbjct: 2   TELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTF 61

Query: 213 PKDYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETII 392
           PKDYP  PPKM+FITEIWHPN+  +G+VCISILHEPGED+YGYEKPEERWLP+HTVETI+
Sbjct: 62  PKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIM 121

Query: 393 TSVISMLAEPNADSPANVDAAKDFRENFS-EFKRKVQRCVRRSQE 524
            SVISMLA+PN DSPANVDAAK++RE+ + EFKRKV RCVR+SQE
Sbjct: 122 ISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQE 166

>sp|P34477|UBC7_CAEEL Probable ubiquitin-conjugating enzyme E2 7 (Ubiquitin-protein
           ligase 7) (Ubiquitin carrier protein 7)
          Length = 164

 Score =  255 bits (652), Expect = 6e-68
 Identities = 116/160 (72%), Positives = 141/160 (88%)
 Frame = +3

Query: 45  SVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFPKDY 224
           S LLL+KQLA++ +   +GFSAGLVDD +I+KW+VL+IGPPDTLYEGG+FKA LDFP+DY
Sbjct: 4   SSLLLKKQLADMRRVPVDGFSAGLVDDNDIYKWEVLVIGPPDTLYEGGFFKAILDFPRDY 63

Query: 225 PNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIITSVI 404
           P  PPKM+FI+EIWHPNI   G VCISILH+PG+D++GYE+PEERWLPVHTVETI+ SVI
Sbjct: 64  PQKPPKMKFISEIWHPNIDKEGNVCISILHDPGDDKWGYERPEERWLPVHTVETILLSVI 123

Query: 405 SMLAEPNADSPANVDAAKDFRENFSEFKRKVQRCVRRSQE 524
           SML +PN +SPANVDAAK  REN++EFK+KV +CVRRSQE
Sbjct: 124 SMLTDPNFESPANVDAAKMQRENYAEFKKKVAQCVRRSQE 163

>sp|Q9Y818|UBC15_SCHPO Ubiquitin-conjugating enzyme E2 15 (Ubiquitin-protein ligase 15)
           (Ubiquitin carrier protein 15)
          Length = 167

 Score =  236 bits (603), Expect = 3e-62
 Identities = 110/163 (67%), Positives = 135/163 (82%)
 Frame = +3

Query: 36  NSASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFP 215
           +SAS  LL+KQL E+ KN  +GFS GLVDD++IF+W+V+IIGP DTLYEGG+F A L FP
Sbjct: 3   SSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFP 62

Query: 216 KDYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIIT 395
           +DYP  PPKM+F TEIWHPN+  +GEVCISILH PG+D+YGYE   ERWLPVH+ ETI+ 
Sbjct: 63  QDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILI 122

Query: 396 SVISMLAEPNADSPANVDAAKDFRENFSEFKRKVQRCVRRSQE 524
           SVISML+ PN +SPAN+DAAK+FREN  EFK++V+R VRRS E
Sbjct: 123 SVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRRSIE 165

>sp|Q42541|UBCD_ARATH Ubiquitin-conjugating enzyme E2 13 (Ubiquitin-protein ligase 13)
           (Ubiquitin carrier protein 13)
          Length = 166

 Score =  228 bits (582), Expect = 8e-60
 Identities = 106/165 (64%), Positives = 132/165 (80%)
 Frame = +3

Query: 36  NSASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFP 215
           NS + LLLQKQL +L K+  +GFSAGLVD++NIF+W V IIGPPDTLYEGG+F A + FP
Sbjct: 2   NSQACLLLQKQLKDLCKHPVDGFSAGLVDEKNIFEWSVTIIGPPDTLYEGGFFYAIMSFP 61

Query: 216 KDYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIIT 395
           ++YPN PP +RF ++IWHPN+   G VCISILH PG+D  GYE   ERW PVHTVE+I+ 
Sbjct: 62  QNYPNSPPTVRFTSDIWHPNVYPDGRVCISILHPPGDDPSGYELASERWTPVHTVESIML 121

Query: 396 SVISMLAEPNADSPANVDAAKDFRENFSEFKRKVQRCVRRSQECF 530
           S+ISML+ PN +SPANV+AAK++RE   EFK+KV RCVR+SQE F
Sbjct: 122 SIISMLSGPNDESPANVEAAKEWREKRDEFKKKVSRCVRKSQEMF 166

>sp|Q42540|UBC7_ARATH Ubiquitin-conjugating enzyme E2 7 (Ubiquitin-protein ligase 7)
           (Ubiquitin carrier protein 7)
          Length = 166

 Score =  226 bits (575), Expect = 5e-59
 Identities = 104/164 (63%), Positives = 132/164 (80%)
 Frame = +3

Query: 39  SASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFPK 218
           S + LLLQKQL +L K+  +GFSAGLVD++NIF+W V IIGPPDTLYEGG+F A + FP+
Sbjct: 3   SQASLLLQKQLKDLCKHPVDGFSAGLVDEKNIFEWSVTIIGPPDTLYEGGFFNAIMTFPQ 62

Query: 219 DYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIITS 398
           +YPN PP +RF +++WHPN+ S G VCISILH PG+D  GYE   ERW PVHTVE+I+ S
Sbjct: 63  NYPNSPPTVRFTSDMWHPNVYSDGRVCISILHPPGDDPSGYELASERWTPVHTVESIMLS 122

Query: 399 VISMLAEPNADSPANVDAAKDFRENFSEFKRKVQRCVRRSQECF 530
           +ISML+ PN +SPANV+AAK++R+   EFK+KV RCVR+SQE F
Sbjct: 123 IISMLSGPNDESPANVEAAKEWRDKRDEFKKKVSRCVRKSQEMF 166

>sp|P42747|UBC14_ARATH Ubiquitin-conjugating enzyme E2 14 (Ubiquitin-protein ligase 14)
           (Ubiquitin carrier protein 14) (TAYO29)
          Length = 167

 Score =  224 bits (572), Expect = 1e-58
 Identities = 104/166 (62%), Positives = 134/166 (80%)
 Frame = +3

Query: 27  MTTNSASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAEL 206
           M  N AS LLLQKQL +L K   +GFSAGLVD++N+F+W V I+GPPDTLYEGG+F A +
Sbjct: 1   MANNQAS-LLLQKQLKDLCKKPVDGFSAGLVDEKNVFQWSVSIMGPPDTLYEGGFFNAIM 59

Query: 207 DFPKDYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVET 386
            FP++YP  PP + F +E+WHPN+ S G+VCISILH PG+D +GYE   ERW PVHTVE+
Sbjct: 60  SFPENYPVSPPTVTFTSEMWHPNVYSDGKVCISILHPPGDDPHGYELASERWTPVHTVES 119

Query: 387 IITSVISMLAEPNADSPANVDAAKDFRENFSEFKRKVQRCVRRSQE 524
           I+ S+ISML+ PN +SPANV+AAK++R+N +EF++KV RCVRRSQE
Sbjct: 120 IVLSIISMLSGPNDESPANVEAAKEWRDNRAEFRKKVSRCVRRSQE 165

>sp|P25868|UBC7_WHEAT Ubiquitin-conjugating enzyme E2 7 (Ubiquitin-protein ligase 7)
           (Ubiquitin carrier protein 7)
          Length = 168

 Score =  200 bits (509), Expect = 2e-51
 Identities = 90/144 (62%), Positives = 113/144 (78%)
 Frame = +3

Query: 93  SEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFPKDYPNHPPKMRFITEIWHP 272
           S GF  G VDD N+F+WQV IIGPP+TLY+GGYF A + FP++YPN PP +RF +E+WHP
Sbjct: 24  SMGFQLGFVDDSNVFEWQVTIIGPPETLYDGGYFNAIMSFPQNYPNSPPTVRFTSEMWHP 83

Query: 273 NIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIITSVISMLAEPNADSPANVDA 452
           N+   G VCISI H PG+D  GYE   ERW PVHTVE+I+ S+ISML+ PN +SPAN++A
Sbjct: 84  NVYPDGRVCISI-HPPGDDPNGYELASERWTPVHTVESIVLSIISMLSSPNDESPANIEA 142

Query: 453 AKDFRENFSEFKRKVQRCVRRSQE 524
           AKD+RE   EFK+KV+R VR+SQE
Sbjct: 143 AKDWREKQDEFKKKVRRAVRKSQE 166

>sp|P60605|UB2G2_MOUSE Ubiquitin-conjugating enzyme E2 G2 (Ubiquitin-protein ligase G2)
           (Ubiquitin carrier protein G2)
 sp|P60604|UB2G2_HUMAN Ubiquitin-conjugating enzyme E2 G2 (Ubiquitin-protein ligase G2)
           (Ubiquitin carrier protein G2)
          Length = 165

 Score =  165 bits (418), Expect = 9e-41
 Identities = 80/154 (51%), Positives = 106/154 (68%)
 Frame = +3

Query: 57  LQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFPKDYPNHP 236
           L  +  +L  N  EG  AG +++EN F+W+ LI+GP DT +E G F A L FP DYP  P
Sbjct: 9   LMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSP 68

Query: 237 PKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIITSVISMLA 416
           PKMRF  E++HPNI   G VCISILH PG+D  GYE   ERW PV +VE I+ SV+SMLA
Sbjct: 69  PKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLA 128

Query: 417 EPNADSPANVDAAKDFRENFSEFKRKVQRCVRRS 518
           EPN +S ANVDA+K +R++  +F +  ++ V++S
Sbjct: 129 EPNDESGANVDASKMWRDDREQFYKIAKQIVQKS 162

>sp|Q29503|UBC3_RABIT Ubiquitin-conjugating enzyme E2-32 kDa complementing
           (Ubiquitin-protein ligase) (Ubiquitin carrier protein)
           (E2-CDC34)
          Length = 238

 Score =  164 bits (415), Expect = 2e-40
 Identities = 79/146 (54%), Positives = 100/146 (68%)
 Frame = +3

Query: 33  TNSASVLLLQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDF 212
           T+S   L+L+  L  L +   EGF   LVD+ +++ W+V I GPP+TLYEGGYFKA + F
Sbjct: 7   TSSQKALMLE--LKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKF 64

Query: 213 PKDYPNHPPKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETII 392
           P DYP  PP  RF+T++WHPNI  +G+VCISILH P +D    E P ERW P   V TI+
Sbjct: 65  PIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTIL 124

Query: 393 TSVISMLAEPNADSPANVDAAKDFRE 470
            SVIS+L EPN  SPANVDA+  FR+
Sbjct: 125 LSVISLLNEPNTFSPANVDASVMFRK 150

>sp|Q02159|UBC7_YEAST Ubiquitin-conjugating enzyme E2-18 kDa (Ubiquitin-protein ligase)
           (Ubiquitin carrier protein)
          Length = 165

 Score =  163 bits (412), Expect = 4e-40
 Identities = 82/154 (53%), Positives = 104/154 (67%)
 Frame = +3

Query: 57  LQKQLAELNKNGSEGFSAGLVDDENIFKWQVLIIGPPDTLYEGGYFKAELDFPKDYPNHP 236
           L K+L +L K+   G  AG   + NIF W  LI GPPDT Y  G F A+L+FPKDYP  P
Sbjct: 9   LLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSP 68

Query: 237 PKMRFITEIWHPNIGSSGEVCISILHEPGEDRYGYEKPEERWLPVHTVETIITSVISMLA 416
           PK+ F   I HPNI  +GEVCISILH PG+D   YE  EERW PV +VE I+ SV+SML+
Sbjct: 69  PKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLS 128

Query: 417 EPNADSPANVDAAKDFRENFSEFKRKVQRCVRRS 518
           EPN +S AN+DA   +R+N  EF+R+V+  + +S
Sbjct: 129 EPNIESGANIDACILWRDNRPEFERQVKLSILKS 162

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,076,412
Number of Sequences: 369166
Number of extensions: 1268065
Number of successful extensions: 3463
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3312
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3383
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5511356910
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00039

  1. Dr_sW_001_P16
  2. Dr_sW_018_K17