Planaria EST Database

DrC_00032

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00032
         (431 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q57565|SRP54_METJA  Signal recognition 54 kDa protein (SR...    34   0.15 
sp|P29055|TF2B_YEAST  Transcription initiation factor IIB (G...    32   0.57 
sp|Q09725|MIS4_SCHPO  Sister chromatid cohesion protein mis4...    32   0.75 
sp|O67298|SYM_AQUAE  Methionyl-tRNA synthetase (Methionine--...    31   1.3  
sp|Q75DE8|ASK1_ASHGO  DASH complex subunit ASK1 (Outer kinet...    31   1.3  
sp|P08547|LIN1_HUMAN  LINE-1 reverse transcriptase homolog         31   1.3  
sp|O67192|GCSH4_AQUAE  Glycine cleavage system H protein 4         30   1.7  
sp|P39768|OPA_DROME  Pair-rule protein odd-paired                  30   2.2  
sp|P40450|BNR1_YEAST  BNI1-related protein 1                       30   2.2  
sp|Q10411|SPO15_SCHPO  Sporulation-specific protein 15             30   2.8  

>sp|Q57565|SRP54_METJA Signal recognition 54 kDa protein (SRP54)
          Length = 451

 Score = 33.9 bits (76), Expect = 0.15
 Identities = 28/97 (28%), Positives = 51/97 (52%), Gaps = 5/97 (5%)
 Frame = +2

Query: 104 LDKLNDDFNKAGDKIDADCDAAYL--KILKDIVPAYKPLEDKCGIYVE---KMSKECHAE 268
           LDKL ++ NKA +K+ A   AA++  K++K+++   +    +  + V+   KMSKE   E
Sbjct: 2   LDKLGENLNKALNKLKA---AAFVDKKLIKEVIKDIQRALIQADVNVKLVLKMSKE--IE 56

Query: 269 RKEMEDSVARDIDKLDSSCDAEYAKTVKEHNKAKDKL 379
           R+ +E+   + + K +      Y + VK   +   KL
Sbjct: 57  RRALEEKTPKGLSKKEHIIKIVYEELVKLLGEEAKKL 93

>sp|P29055|TF2B_YEAST Transcription initiation factor IIB (General transcription factor
           TFIIB) (Transcription factor E)
          Length = 345

 Score = 32.0 bits (71), Expect = 0.57
 Identities = 36/113 (31%), Positives = 49/113 (43%), Gaps = 10/113 (8%)
 Frame = +2

Query: 65  FQKRALSAECQKELDKLNDDFNKAGDKIDADCDAAYL-KILKDIVPAYKPLEDKCGIYVE 241
           F K    A+ +  +DK +++   A  KI   CDAA L KI+KD                +
Sbjct: 106 FTKELNKAQGKNVMDKKDNEVQAAFAKITMLCDAAELPKIVKDC--------------AK 151

Query: 242 KMSKECHAER----KEMEDSVARDIDKLDSSCDAEYAKTVKE-----HNKAKD 373
           +  K CH E+    K ME  +A  I  L     AE A+T KE     H K K+
Sbjct: 152 EAYKLCHDEKTLKGKSMESIMAASI--LIGCRRAEVARTFKEIQSLIHVKTKE 202

>sp|Q09725|MIS4_SCHPO Sister chromatid cohesion protein mis4 (SCC2 homolog)
          Length = 1583

 Score = 31.6 bits (70), Expect = 0.75
 Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
 Frame = +2

Query: 101  ELDKLNDDFNKAGDKIDADCDAAYLKILKDIVPAYKPLEDKCGIY-VEKMSKECHAE 268
            +L+++NDD+  + D I  +CD AY+ +L       K  + +  I+ ++ MS+ C  E
Sbjct: 1158 DLNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSRICLRE 1214

>sp|O67298|SYM_AQUAE Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
          Length = 497

 Score = 30.8 bits (68), Expect = 1.3
 Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 4/86 (4%)
 Frame = +2

Query: 146 IDADCDAAYLKILKDIVPAYKPLEDKCGIYVEKMSKECHAERKEMEDSVARDIDKLDSSC 325
           I AD  A Y + L+D    +    D+ G+ ++K ++E     KE+ D  A    KL    
Sbjct: 29  IAADTIARYYR-LRDYDVFFLTGTDEHGLKIQKKAEELGISPKELVDRNAERFKKLWEFL 87

Query: 326 DAEYAKTVKE----HNKAKDKLLAEC 391
             EY K ++     H K   K+  EC
Sbjct: 88  KIEYTKFIRTTDPYHVKFVQKVFEEC 113

>sp|Q75DE8|ASK1_ASHGO DASH complex subunit ASK1 (Outer kinetochore protein ASK1)
           (Associated with spindles and kinetochores protein 1)
          Length = 181

 Score = 30.8 bits (68), Expect = 1.3
 Identities = 14/33 (42%), Positives = 21/33 (63%)
 Frame = +2

Query: 104 LDKLNDDFNKAGDKIDADCDAAYLKILKDIVPA 202
           L++L+ +      KIDA+  A + KI KDI+PA
Sbjct: 12  LEQLDQEITLQLQKIDANLSACFSKITKDIIPA 44

>sp|P08547|LIN1_HUMAN LINE-1 reverse transcriptase homolog
          Length = 1259

 Score = 30.8 bits (68), Expect = 1.3
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +3

Query: 342 KPLKNIIKQKINCWQNVPCLVKFKIN 419
           KPL N IK+  N W+N+PC    +IN
Sbjct: 785 KPLLNEIKEDTNKWKNIPCSWVGRIN 810

>sp|O67192|GCSH4_AQUAE Glycine cleavage system H protein 4
          Length = 171

 Score = 30.4 bits (67), Expect = 1.7
 Identities = 29/116 (25%), Positives = 55/116 (47%), Gaps = 13/116 (11%)
 Frame = +2

Query: 65  FQKRALSAECQKEL-DKLNDDFNKAGDKIDADCDAAYLKILKDIVPAYKP---------- 211
           F +  ++   QK+L D +  D  + G ++DA    A ++ +K++ P Y P          
Sbjct: 30  FAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPVTGTVVEVNE 89

Query: 212 -LEDKCGIYVEKMSKECHAERKEMEDSVARDIDKLDSSCD-AEYAKTVKEHNKAKD 373
            L+D+ GI  +   +       EM+D    +++ L ++ D AEY K + E  K ++
Sbjct: 90  DLKDEPGIINDDPYEAGWIAVIEMKDPT--EVEDLMTAQDYAEYLKEIVEEEKEEE 143

>sp|P39768|OPA_DROME Pair-rule protein odd-paired
          Length = 609

 Score = 30.0 bits (66), Expect = 2.2
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = -1

Query: 176 SNTLHHNPHQFYHQPY*NHHSIYQVPFDILHSTLASGNS 60
           ++ LHHN H  YH P   HH     P D  H+T  SG++
Sbjct: 549 ASMLHHNHHLLYH-PAAQHHP----PSDWYHTTAPSGSA 582

>sp|P40450|BNR1_YEAST BNI1-related protein 1
          Length = 1375

 Score = 30.0 bits (66), Expect = 2.2
 Identities = 39/151 (25%), Positives = 65/151 (43%), Gaps = 20/151 (13%)
 Frame = +2

Query: 29   LFLYTECGKLTSFQKRALSAECQKELDKLNDDFNKAGDKIDADCDAAYLKILKDIVPAYK 208
            +F+ TE G      KR +     K L KL      A  +   D + ++L  ++ ++    
Sbjct: 1094 MFIITEIGN--HMNKRIVKGIKLKSLTKL------AFVRSSIDQNVSFLHFIEKVIRIKY 1145

Query: 209  P-----------LEDKCGIYVEKMSKECHAERKEMEDSVAR-DIDKLDSSCDA----EYA 340
            P           +ED   I +E +  ECH   K++ED V +  + KL    +     +  
Sbjct: 1146 PDIYGFVDDLKNIEDLGKISLEHVESECHEFHKKIEDLVTQFQVGKLSKEENLDPRDQII 1205

Query: 341  KTVK-EHNKAKDK---LLAECAMSR*I*NKL 421
            K VK + N+AK K   L+ +C ++    NKL
Sbjct: 1206 KKVKFKINRAKTKSELLIGQCKLTLIDLNKL 1236

>sp|Q10411|SPO15_SCHPO Sporulation-specific protein 15
          Length = 1957

 Score = 29.6 bits (65), Expect = 2.8
 Identities = 21/94 (22%), Positives = 42/94 (44%), Gaps = 9/94 (9%)
 Frame = +2

Query: 122  DFNKAGDKIDADCDAAYLKILKDIVPAYKPLEDKCGIY------VEKMSKECHAERKEME 283
            D +   D +  D     L+ L+ +  +   L+ +C I       ++ +     A   E+E
Sbjct: 1633 DLSNIKDSLSED-----LRTLRSLEDSVASLQKECKIKSNTVESLQDVLTSVQARNAELE 1687

Query: 284  DSVARDIDKL---DSSCDAEYAKTVKEHNKAKDK 376
            D V+R +DK+   D  C+    K  K H++ +++
Sbjct: 1688 DEVSRSVDKIRRRDDRCEHLSGKLKKLHSQLEEQ 1721

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,520,315
Number of Sequences: 369166
Number of extensions: 753760
Number of successful extensions: 2855
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2839
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2144903640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00032

  1. Dr_sW_001_N03
  2. Dr_sW_007_D03