Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_N03
(431 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q57565|SRP54_METJA Signal recognition 54 kDa protein (SR... 33 0.26
sp|P40450|BNR1_YEAST BNI1-related protein 1 32 0.75
sp|Q09725|MIS4_SCHPO Sister chromatid cohesion protein mis4... 31 0.97
sp|O67192|GCSH4_AQUAE Glycine cleavage system H protein 4 31 0.97
sp|Q9H9E3|COG4_HUMAN Conserved oligomeric Golgi complex com... 31 1.3
sp|O78481|EFTS_GUITH Elongation factor Ts (EF-Ts) 31 1.3
sp|P08547|LIN1_HUMAN LINE-1 reverse transcriptase homolog 31 1.3
sp|P29055|TF2B_YEAST Transcription initiation factor IIB (G... 30 1.7
sp|P28025|EG51_XENLA Kinesin-related motor protein Eg5 1 30 1.7
sp|P39768|OPA_DROME Pair-rule protein odd-paired 30 2.2
>sp|Q57565|SRP54_METJA Signal recognition 54 kDa protein (SRP54)
Length = 451
Score = 33.1 bits (74), Expect = 0.26
Identities = 27/95 (28%), Positives = 48/95 (50%), Gaps = 3/95 (3%)
Frame = +2
Query: 104 LDKLNDDFNKAGDKIDADCDVAYLKILKDIVPAYKPLEDKCGIYVE---KMSKECHAERK 274
LDKL ++ NKA +K+ A V K++K+++ + + + V+ KMSKE ER+
Sbjct: 2 LDKLGENLNKALNKLKAAAFVD-KKLIKEVIKDIQRALIQADVNVKLVLKMSKE--IERR 58
Query: 275 EMEDSVARDIDKLDSSCDAEYAKTVKEHNKAKDKL 379
+E+ + + K + Y + VK + KL
Sbjct: 59 ALEEKTPKGLSKKEHIIKIVYEELVKLLGEEAKKL 93
>sp|P40450|BNR1_YEAST BNI1-related protein 1
Length = 1375
Score = 31.6 bits (70), Expect = 0.75
Identities = 40/151 (26%), Positives = 66/151 (43%), Gaps = 20/151 (13%)
Frame = +2
Query: 29 LFLYTECGKLTSFQKRALSAECQKELDKLNDDFNKAGDKIDADCDVAYLKILKDIVPAYK 208
+F+ TE G KR + K L KL A + D +V++L ++ ++
Sbjct: 1094 MFIITEIGN--HMNKRIVKGIKLKSLTKL------AFVRSSIDQNVSFLHFIEKVIRIKY 1145
Query: 209 P-----------LEDKCGIYVEKMSKECHAERKEMEDSVAR-DIDKLDSSCDA----EYA 340
P +ED I +E + ECH K++ED V + + KL + +
Sbjct: 1146 PDIYGFVDDLKNIEDLGKISLEHVESECHEFHKKIEDLVTQFQVGKLSKEENLDPRDQII 1205
Query: 341 KTVK-EHNKAKDK---LLAECAMSR*I*NKL 421
K VK + N+AK K L+ +C ++ NKL
Sbjct: 1206 KKVKFKINRAKTKSELLIGQCKLTLIDLNKL 1236
>sp|Q09725|MIS4_SCHPO Sister chromatid cohesion protein mis4 (SCC2 homolog)
Length = 1583
Score = 31.2 bits (69), Expect = 0.97
Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +2
Query: 101 ELDKLNDDFNKAGDKIDADCDVAYLKILKDIVPAYKPLEDKCGIY-VEKMSKECHAE 268
+L+++NDD+ + D I +CD AY+ +L K + + I+ ++ MS+ C E
Sbjct: 1158 DLNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSRICLRE 1214
>sp|O67192|GCSH4_AQUAE Glycine cleavage system H protein 4
Length = 171
Score = 31.2 bits (69), Expect = 0.97
Identities = 29/116 (25%), Positives = 56/116 (48%), Gaps = 13/116 (11%)
Frame = +2
Query: 65 FQKRALSAECQKEL-DKLNDDFNKAGDKIDADCDVAYLKILKDIVPAYKP---------- 211
F + ++ QK+L D + D + G ++DA +A ++ +K++ P Y P
Sbjct: 30 FAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPVTGTVVEVNE 89
Query: 212 -LEDKCGIYVEKMSKECHAERKEMEDSVARDIDKLDSSCD-AEYAKTVKEHNKAKD 373
L+D+ GI + + EM+D +++ L ++ D AEY K + E K ++
Sbjct: 90 DLKDEPGIINDDPYEAGWIAVIEMKDPT--EVEDLMTAQDYAEYLKEIVEEEKEEE 143
>sp|Q9H9E3|COG4_HUMAN Conserved oligomeric Golgi complex component 4
Length = 785
Score = 30.8 bits (68), Expect = 1.3
Identities = 27/108 (25%), Positives = 43/108 (39%), Gaps = 1/108 (0%)
Frame = +2
Query: 80 LSAECQKELDKLNDDFNKAGDKIDADCDVAYLKILKDIVPAYKPLE-DKCGIYVEKMSKE 256
L EC ++++K+ D F K D V + +P E D V M+
Sbjct: 306 LQVECDRQVEKVVDKFIKQRDYHQQFRHVQNNLMRNSTTEKIEpreLDPILTEVTLMNAR 365
Query: 257 CHAERKEMEDSVARDIDKLDSSCDAEYAKTVKEHNKAKDKLLAECAMS 400
+ ++ ++ D + DS E + EH K DKLL C +S
Sbjct: 366 SELYLRFLKKRISSDFEVGDSMASEEVKQ---EHQKCLDKLLNNCLLS 410
>sp|O78481|EFTS_GUITH Elongation factor Ts (EF-Ts)
Length = 219
Score = 30.8 bits (68), Expect = 1.3
Identities = 24/92 (26%), Positives = 43/92 (46%), Gaps = 6/92 (6%)
Frame = +2
Query: 89 ECQKELDKLNDDFNKAGDKIDADCDVAYLK-----ILKDIVPAYKPLEDKCGIYVEKMSK 253
+C+K L + N DF KA + + V+ K + I+ +Y + K GI VE +
Sbjct: 23 DCKKALQECNGDFEKAVETLKQKGLVSAAKKAARVATEGIIESYIHMGGKLGILVELNCE 82
Query: 254 ECHAERKEMEDSVARDIDKLDSSC-DAEYAKT 346
R+ +A++I ++C + +Y KT
Sbjct: 83 TDFVARRPEFQELAKNIAMQIAACPNVDYIKT 114
>sp|P08547|LIN1_HUMAN LINE-1 reverse transcriptase homolog
Length = 1259
Score = 30.8 bits (68), Expect = 1.3
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 342 KPLKNIIKQKINCWQNVPCLVKFKIN 419
KPL N IK+ N W+N+PC +IN
Sbjct: 785 KPLLNEIKEDTNKWKNIPCSWVGRIN 810
>sp|P29055|TF2B_YEAST Transcription initiation factor IIB (General transcription factor
TFIIB) (Transcription factor E)
Length = 345
Score = 30.4 bits (67), Expect = 1.7
Identities = 35/113 (30%), Positives = 48/113 (42%), Gaps = 10/113 (8%)
Frame = +2
Query: 65 FQKRALSAECQKELDKLNDDFNKAGDKIDADCDVAYL-KILKDIVPAYKPLEDKCGIYVE 241
F K A+ + +DK +++ A KI CD A L KI+KD +
Sbjct: 106 FTKELNKAQGKNVMDKKDNEVQAAFAKITMLCDAAELPKIVKDC--------------AK 151
Query: 242 KMSKECHAER----KEMEDSVARDIDKLDSSCDAEYAKTVKE-----HNKAKD 373
+ K CH E+ K ME +A I L AE A+T KE H K K+
Sbjct: 152 EAYKLCHDEKTLKGKSMESIMAASI--LIGCRRAEVARTFKEIQSLIHVKTKE 202
>sp|P28025|EG51_XENLA Kinesin-related motor protein Eg5 1
Length = 1060
Score = 30.4 bits (67), Expect = 1.7
Identities = 26/93 (27%), Positives = 47/93 (50%), Gaps = 3/93 (3%)
Frame = +2
Query: 44 ECGKLTSFQKRALSAECQKELDKLNDDFNKAGDKIDADCD--VAYL-KILKDIVPAYKPL 214
+C KLTS +S E Q+ + AG KID+ + V YL K + +K +
Sbjct: 804 QCEKLTS-SINTISQESQQWCES-------AGQKIDSVLEEQVCYLHSSKKHLQNLHKGV 855
Query: 215 EDKCGIYVEKMSKECHAERKEMEDSVARDIDKL 313
ED CG V +++ +A+R+ E ++ ++++
Sbjct: 856 EDSCGSSVVEITDRVNAQRQAEEKALTSLVEQV 888
>sp|P39768|OPA_DROME Pair-rule protein odd-paired
Length = 609
Score = 30.0 bits (66), Expect = 2.2
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 176 SNTLHHNPHQFYHQPY*NHHSIYQVPFDILHSTLASGNS 60
++ LHHN H YH P HH P D H+T SG++
Sbjct: 549 ASMLHHNHHLLYH-PAAQHHP----PSDWYHTTAPSGSA 582
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,413,991
Number of Sequences: 369166
Number of extensions: 751882
Number of successful extensions: 2825
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2809
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2144903640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)