Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_D03
(400 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q57565|SRP54_METJA Signal recognition 54 kDa protein (SR... 34 0.12
sp|P29055|TF2B_YEAST Transcription initiation factor IIB (G... 32 0.46
sp|Q09725|MIS4_SCHPO Sister chromatid cohesion protein mis4... 32 0.59
sp|O67298|SYM_AQUAE Methionyl-tRNA synthetase (Methionine--... 31 1.0
sp|Q75DE8|ASK1_ASHGO DASH complex subunit ASK1 (Outer kinet... 31 1.0
sp|P08547|LIN1_HUMAN LINE-1 reverse transcriptase homolog 31 1.0
sp|O67192|GCSH4_AQUAE Glycine cleavage system H protein 4 30 1.3
sp|P39768|OPA_DROME Pair-rule protein odd-paired 30 1.7
sp|Q10411|SPO15_SCHPO Sporulation-specific protein 15 30 2.3
sp|Q7VIN8|TIG_HELHP Trigger factor (TF) 30 2.3
>sp|Q57565|SRP54_METJA Signal recognition 54 kDa protein (SRP54)
Length = 451
Score = 33.9 bits (76), Expect = 0.12
Identities = 28/97 (28%), Positives = 51/97 (52%), Gaps = 5/97 (5%)
Frame = +1
Query: 73 LDKLNDDFNKAGDKIDADCDAAYL--KILKDIVPAYKPLEDKCGIYVE---KMSKECHAE 237
LDKL ++ NKA +K+ A AA++ K++K+++ + + + V+ KMSKE E
Sbjct: 2 LDKLGENLNKALNKLKA---AAFVDKKLIKEVIKDIQRALIQADVNVKLVLKMSKE--IE 56
Query: 238 RKEMEDSVARDIDKLDSSCDAEYAKTVKEHNKAKDKL 348
R+ +E+ + + K + Y + VK + KL
Sbjct: 57 RRALEEKTPKGLSKKEHIIKIVYEELVKLLGEEAKKL 93
>sp|P29055|TF2B_YEAST Transcription initiation factor IIB (General transcription factor
TFIIB) (Transcription factor E)
Length = 345
Score = 32.0 bits (71), Expect = 0.46
Identities = 36/113 (31%), Positives = 49/113 (43%), Gaps = 10/113 (8%)
Frame = +1
Query: 34 FQKRALSAECQKELDKLNDDFNKAGDKIDADCDAAYL-KILKDIVPAYKPLEDKCGIYVE 210
F K A+ + +DK +++ A KI CDAA L KI+KD +
Sbjct: 106 FTKELNKAQGKNVMDKKDNEVQAAFAKITMLCDAAELPKIVKDC--------------AK 151
Query: 211 KMSKECHAER----KEMEDSVARDIDKLDSSCDAEYAKTVKE-----HNKAKD 342
+ K CH E+ K ME +A I L AE A+T KE H K K+
Sbjct: 152 EAYKLCHDEKTLKGKSMESIMAASI--LIGCRRAEVARTFKEIQSLIHVKTKE 202
>sp|Q09725|MIS4_SCHPO Sister chromatid cohesion protein mis4 (SCC2 homolog)
Length = 1583
Score = 31.6 bits (70), Expect = 0.59
Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +1
Query: 70 ELDKLNDDFNKAGDKIDADCDAAYLKILKDIVPAYKPLEDKCGIY-VEKMSKECHAE 237
+L+++NDD+ + D I +CD AY+ +L K + + I+ ++ MS+ C E
Sbjct: 1158 DLNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSRICLRE 1214
>sp|O67298|SYM_AQUAE Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 497
Score = 30.8 bits (68), Expect = 1.0
Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 4/86 (4%)
Frame = +1
Query: 115 IDADCDAAYLKILKDIVPAYKPLEDKCGIYVEKMSKECHAERKEMEDSVARDIDKLDSSC 294
I AD A Y + L+D + D+ G+ ++K ++E KE+ D A KL
Sbjct: 29 IAADTIARYYR-LRDYDVFFLTGTDEHGLKIQKKAEELGISPKELVDRNAERFKKLWEFL 87
Query: 295 DAEYAKTVKE----HNKAKDKLLAEC 360
EY K ++ H K K+ EC
Sbjct: 88 KIEYTKFIRTTDPYHVKFVQKVFEEC 113
>sp|Q75DE8|ASK1_ASHGO DASH complex subunit ASK1 (Outer kinetochore protein ASK1)
(Associated with spindles and kinetochores protein 1)
Length = 181
Score = 30.8 bits (68), Expect = 1.0
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +1
Query: 73 LDKLNDDFNKAGDKIDADCDAAYLKILKDIVPA 171
L++L+ + KIDA+ A + KI KDI+PA
Sbjct: 12 LEQLDQEITLQLQKIDANLSACFSKITKDIIPA 44
>sp|P08547|LIN1_HUMAN LINE-1 reverse transcriptase homolog
Length = 1259
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +2
Query: 311 KPLKNIIKQKINCWQNVPCLVKFKIN 388
KPL N IK+ N W+N+PC +IN
Sbjct: 785 KPLLNEIKEDTNKWKNIPCSWVGRIN 810
>sp|O67192|GCSH4_AQUAE Glycine cleavage system H protein 4
Length = 171
Score = 30.4 bits (67), Expect = 1.3
Identities = 29/116 (25%), Positives = 55/116 (47%), Gaps = 13/116 (11%)
Frame = +1
Query: 34 FQKRALSAECQKEL-DKLNDDFNKAGDKIDADCDAAYLKILKDIVPAYKP---------- 180
F + ++ QK+L D + D + G ++DA A ++ +K++ P Y P
Sbjct: 30 FAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPVTGTVVEVNE 89
Query: 181 -LEDKCGIYVEKMSKECHAERKEMEDSVARDIDKLDSSCD-AEYAKTVKEHNKAKD 342
L+D+ GI + + EM+D +++ L ++ D AEY K + E K ++
Sbjct: 90 DLKDEPGIINDDPYEAGWIAVIEMKDPT--EVEDLMTAQDYAEYLKEIVEEEKEEE 143
>sp|P39768|OPA_DROME Pair-rule protein odd-paired
Length = 609
Score = 30.0 bits (66), Expect = 1.7
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 145 SNTLHHNPHQFYHQPY*NHHSIYQVPFDILHSTLASGNS 29
++ LHHN H YH P HH P D H+T SG++
Sbjct: 549 ASMLHHNHHLLYH-PAAQHHP----PSDWYHTTAPSGSA 582
>sp|Q10411|SPO15_SCHPO Sporulation-specific protein 15
Length = 1957
Score = 29.6 bits (65), Expect = 2.3
Identities = 21/94 (22%), Positives = 42/94 (44%), Gaps = 9/94 (9%)
Frame = +1
Query: 91 DFNKAGDKIDADCDAAYLKILKDIVPAYKPLEDKCGIY------VEKMSKECHAERKEME 252
D + D + D L+ L+ + + L+ +C I ++ + A E+E
Sbjct: 1633 DLSNIKDSLSED-----LRTLRSLEDSVASLQKECKIKSNTVESLQDVLTSVQARNAELE 1687
Query: 253 DSVARDIDKL---DSSCDAEYAKTVKEHNKAKDK 345
D V+R +DK+ D C+ K K H++ +++
Sbjct: 1688 DEVSRSVDKIRRRDDRCEHLSGKLKKLHSQLEEQ 1721
>sp|Q7VIN8|TIG_HELHP Trigger factor (TF)
Length = 442
Score = 29.6 bits (65), Expect = 2.3
Identities = 23/71 (32%), Positives = 38/71 (53%), Gaps = 2/71 (2%)
Frame = +1
Query: 19 GKLTSFQKRALSAECQKELDKLNDDFNK--AGDKIDADCDAAYLKILKDIVPAYKPLEDK 192
GK SF L QKEL K++D+F K AG++ + L+ LKD++ +E K
Sbjct: 232 GKEASFDV-TLHKILQKELPKIDDEFAKSIAGEE-------SNLQSLKDMIKEQLEMEQK 283
Query: 193 CGIYVEKMSKE 225
IY +++ ++
Sbjct: 284 TEIYNKELKEK 294
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,293,648
Number of Sequences: 369166
Number of extensions: 697615
Number of successful extensions: 2673
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2657
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1708532300
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)