Planaria EST Database


DrC_03031

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_03031
         (369 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q17607|MCE1_CAEEL  mRNA capping enzyme [Includes: Polynuc...    69   3e-12
sp|O60942|MCE1_HUMAN  mRNA capping enzyme (HCE) (HCAP1) [Inc...    65   5e-11
sp|O55236|MCE1_MOUSE  mRNA capping enzyme (HCE) (MCE1) [Incl...    63   2e-10
sp|Q6CWR0|MCE1_KLULA  mRNA capping enzyme alpha subunit (mRN...    49   4e-06
sp|Q6FQ31|MCE1_CANGA  mRNA capping enzyme alpha subunit (mRN...    47   1e-05
sp|Q755D0|MCE1_ASHGO  mRNA capping enzyme alpha subunit (mRN...    47   2e-05
sp|P40997|MCE1_SCHPO  mRNA capping enzyme alpha subunit (mRN...    45   7e-05
sp|Q6C783|MCE1_YARLI  mRNA capping enzyme alpha subunit (mRN...    45   7e-05
sp|Q7SB53|MCE1_NEUCR  mRNA capping enzyme alpha subunit (mRN...    43   3e-04
sp|Q01159|MCE1_YEAST  mRNA capping enzyme alpha subunit (mRN...    42   4e-04
>sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynucleotide 5'-triphosphatase
           (mRNA 5'-triphosphatase) (TPase); mRNA
           guanylyltransferase (GTP--RNA guanylyltransferase)
           (GTase)]
          Length = 623

 Score = 69.3 bits (168), Expect = 3e-12
 Identities = 39/94 (41%), Positives = 58/94 (61%), Gaps = 4/94 (4%)
 Frame = +1

Query: 1   IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTL--DNKSL--KWKIMRERTD 168
           ++P ++GYL+V      FG ++        KY  KI+ECTL  DN+    +WK MRERTD
Sbjct: 500 MLPEWIGYLFVQNLSDPFGTMK--ATATLKKYHNKIIECTLLVDNQGRPKEWKFMRERTD 557

Query: 169 KSKPNHISTAYSVLESIKHPVTIGHVYNAINFAL 270
           KS PN + TA +V+E++ +PVT  ++   +N AL
Sbjct: 558 KSLPNGLRTAENVVETMVNPVTETYLIEYVNHAL 591
>sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Includes: Polynucleotide
           5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
           guanylyltransferase (GTP--RNA guanylyltransferase)
           (GTase)]
          Length = 597

 Score = 65.1 bits (157), Expect = 5e-11
 Identities = 36/78 (46%), Positives = 48/78 (61%)
 Frame = +1

Query: 1   IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKP 180
           ++P  VG LYV G +  F  I+  K  +  +YD KI+EC  +N S  W  MR+RTDKS P
Sbjct: 481 LLPQNVGLLYVGGYERPFAQIKVTK--ELKQYDNKIIECKFENNS--WVFMRQRTDKSFP 536

Query: 181 NHISTAYSVLESIKHPVT 234
           N  +TA +V  SI +PVT
Sbjct: 537 NAYNTAMAVCNSISNPVT 554
>sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Includes: Polynucleotide
           5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
           guanylyltransferase (GTP--RNA guanylyltransferase)
           (GTase)]
          Length = 597

 Score = 62.8 bits (151), Expect = 2e-10
 Identities = 35/78 (44%), Positives = 47/78 (60%)
 Frame = +1

Query: 1   IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKP 180
           ++P  VG LYV G +  F  I+  K  +  +YD KI+EC  +N S  W  MR+R DKS P
Sbjct: 481 LLPQNVGLLYVGGYERPFAQIKVTK--ELKQYDNKIIECKFENNS--WVFMRQRIDKSFP 536

Query: 181 NHISTAYSVLESIKHPVT 234
           N  +TA +V  SI +PVT
Sbjct: 537 NAYNTAMAVCNSISNPVT 554
>sp|Q6CWR0|MCE1_KLULA mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 466

 Score = 48.9 bits (115), Expect = 4e-06
 Identities = 23/62 (37%), Positives = 36/62 (58%)
 Frame = +1

Query: 76  KPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTIGHVYNA 255
           K   +  +G+IVEC+ D +S  WK++R R DK   NH+S    VLESI   V++  +   
Sbjct: 338 KAMEEPLNGRIVECSKDQESGAWKLLRFRDDKLNGNHVSVVQKVLESIGDSVSLDDLEQV 397

Query: 256 IN 261
           ++
Sbjct: 398 VD 399
>sp|Q6FQ31|MCE1_CANGA mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 449

 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 23/54 (42%), Positives = 32/54 (59%)
 Frame = +1

Query: 97  DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTIGHVYNAI 258
           +G+IVECT D ++  W ++R R DK   NH S    VLESI   VTI  +  ++
Sbjct: 344 NGRIVECTKDPETGSWTMLRFRDDKLNGNHTSVVQKVLESISDSVTIDDLAESV 397
>sp|Q755D0|MCE1_ASHGO mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 463

 Score = 46.6 bits (109), Expect = 2e-05
 Identities = 21/47 (44%), Positives = 29/47 (61%)
 Frame = +1

Query: 97  DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTI 237
           +G+IVEC  D ++ +WK +R R DK   NH+S    VLESI   V +
Sbjct: 344 NGRIVECAKDQETGEWKFLRFRDDKLNGNHVSVVQKVLESISDSVKL 390
>sp|P40997|MCE1_SCHPO mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 402

 Score = 44.7 bits (104), Expect = 7e-05
 Identities = 22/44 (50%), Positives = 32/44 (72%)
 Frame = +1

Query: 103 KIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVT 234
           +IVEC LD+++ +W+ +R R DK   NHIST  SVL+SI+  V+
Sbjct: 310 RIVECYLDDEN-RWRFLRFRDDKRDANHISTVKSVLQSIEDGVS 352
>sp|Q6C783|MCE1_YARLI mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 391

 Score = 44.7 bits (104), Expect = 7e-05
 Identities = 27/83 (32%), Positives = 51/83 (61%)
 Frame = +1

Query: 7   PYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKPNH 186
           PY  G LY+T E+  +  ++ +++P     + ++VEC  D+K  +W+ +R R DK+  N+
Sbjct: 281 PY--GELYLTDEE--WDNLKALEEP----LEERVVECIKDDKK-RWRYLRFRDDKTNANY 331

Query: 187 ISTAYSVLESIKHPVTIGHVYNA 255
           I+T   V++SI  PV+  ++ +A
Sbjct: 332 ITTVEKVIDSIDDPVSEKNLLDA 354
>sp|Q7SB53|MCE1_NEUCR mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 402

 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 20/49 (40%), Positives = 32/49 (65%)
 Frame = +1

Query: 88  DKYDGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVT 234
           D    ++VEC LD ++ +W++ R R DK++ NH ST  SV+ SI+  V+
Sbjct: 322 DPLQDRVVECCLDAEN-RWRLFRFRDDKNEANHTSTVSSVMASIRDGVS 369
>sp|Q01159|MCE1_YEAST mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
           (GTP--RNA guanylyltransferase) (GTase)
          Length = 459

 Score = 42.0 bits (97), Expect = 4e-04
 Identities = 19/47 (40%), Positives = 29/47 (61%)
 Frame = +1

Query: 97  DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTI 237
           +G+IVEC  + ++  W+++R R DK   NH S    VLESI   V++
Sbjct: 348 NGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSVSL 394
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,891,190
Number of Sequences: 369166
Number of extensions: 958784
Number of successful extensions: 2715
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2656
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2710
length of database: 68,354,980
effective HSP length: 89
effective length of database: 51,913,565
effective search space used: 1713147645
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)