Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_H21
(369 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynuc... 69 3e-12
sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Inc... 65 5e-11
sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Incl... 63 2e-10
sp|Q6CWR0|MCE1_KLULA mRNA capping enzyme alpha subunit (mRN... 49 4e-06
sp|Q6FQ31|MCE1_CANGA mRNA capping enzyme alpha subunit (mRN... 47 1e-05
sp|Q755D0|MCE1_ASHGO mRNA capping enzyme alpha subunit (mRN... 47 2e-05
sp|P40997|MCE1_SCHPO mRNA capping enzyme alpha subunit (mRN... 45 7e-05
sp|Q6C783|MCE1_YARLI mRNA capping enzyme alpha subunit (mRN... 45 7e-05
sp|Q7SB53|MCE1_NEUCR mRNA capping enzyme alpha subunit (mRN... 43 3e-04
sp|Q01159|MCE1_YEAST mRNA capping enzyme alpha subunit (mRN... 42 4e-04
>sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynucleotide 5'-triphosphatase
(mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 623
Score = 69.3 bits (168), Expect = 3e-12
Identities = 39/94 (41%), Positives = 58/94 (61%), Gaps = 4/94 (4%)
Frame = +1
Query: 1 IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTL--DNKSL--KWKIMRERTD 168
++P ++GYL+V FG ++ KY KI+ECTL DN+ +WK MRERTD
Sbjct: 500 MLPEWIGYLFVQNLSDPFGTMK--ATATLKKYHNKIIECTLLVDNQGRPKEWKFMRERTD 557
Query: 169 KSKPNHISTAYSVLESIKHPVTIGHVYNAINFAL 270
KS PN + TA +V+E++ +PVT ++ +N AL
Sbjct: 558 KSLPNGLRTAENVVETMVNPVTETYLIEYVNHAL 591
>sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 597
Score = 65.1 bits (157), Expect = 5e-11
Identities = 36/78 (46%), Positives = 48/78 (61%)
Frame = +1
Query: 1 IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKP 180
++P VG LYV G + F I+ K + +YD KI+EC +N S W MR+RTDKS P
Sbjct: 481 LLPQNVGLLYVGGYERPFAQIKVTK--ELKQYDNKIIECKFENNS--WVFMRQRTDKSFP 536
Query: 181 NHISTAYSVLESIKHPVT 234
N +TA +V SI +PVT
Sbjct: 537 NAYNTAMAVCNSISNPVT 554
>sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 597
Score = 62.8 bits (151), Expect = 2e-10
Identities = 35/78 (44%), Positives = 47/78 (60%)
Frame = +1
Query: 1 IVPYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKP 180
++P VG LYV G + F I+ K + +YD KI+EC +N S W MR+R DKS P
Sbjct: 481 LLPQNVGLLYVGGYERPFAQIKVTK--ELKQYDNKIIECKFENNS--WVFMRQRIDKSFP 536
Query: 181 NHISTAYSVLESIKHPVT 234
N +TA +V SI +PVT
Sbjct: 537 NAYNTAMAVCNSISNPVT 554
>sp|Q6CWR0|MCE1_KLULA mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 466
Score = 48.9 bits (115), Expect = 4e-06
Identities = 23/62 (37%), Positives = 36/62 (58%)
Frame = +1
Query: 76 KPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTIGHVYNA 255
K + +G+IVEC+ D +S WK++R R DK NH+S VLESI V++ +
Sbjct: 338 KAMEEPLNGRIVECSKDQESGAWKLLRFRDDKLNGNHVSVVQKVLESIGDSVSLDDLEQV 397
Query: 256 IN 261
++
Sbjct: 398 VD 399
>sp|Q6FQ31|MCE1_CANGA mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 449
Score = 47.4 bits (111), Expect = 1e-05
Identities = 23/54 (42%), Positives = 32/54 (59%)
Frame = +1
Query: 97 DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTIGHVYNAI 258
+G+IVECT D ++ W ++R R DK NH S VLESI VTI + ++
Sbjct: 344 NGRIVECTKDPETGSWTMLRFRDDKLNGNHTSVVQKVLESISDSVTIDDLAESV 397
>sp|Q755D0|MCE1_ASHGO mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 463
Score = 46.6 bits (109), Expect = 2e-05
Identities = 21/47 (44%), Positives = 29/47 (61%)
Frame = +1
Query: 97 DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTI 237
+G+IVEC D ++ +WK +R R DK NH+S VLESI V +
Sbjct: 344 NGRIVECAKDQETGEWKFLRFRDDKLNGNHVSVVQKVLESISDSVKL 390
>sp|P40997|MCE1_SCHPO mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 402
Score = 44.7 bits (104), Expect = 7e-05
Identities = 22/44 (50%), Positives = 32/44 (72%)
Frame = +1
Query: 103 KIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVT 234
+IVEC LD+++ +W+ +R R DK NHIST SVL+SI+ V+
Sbjct: 310 RIVECYLDDEN-RWRFLRFRDDKRDANHISTVKSVLQSIEDGVS 352
>sp|Q6C783|MCE1_YARLI mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 391
Score = 44.7 bits (104), Expect = 7e-05
Identities = 27/83 (32%), Positives = 51/83 (61%)
Frame = +1
Query: 7 PYYVGYLYVTGEKSHFGAIEHIKKPDRDKYDGKIVECTLDNKSLKWKIMRERTDKSKPNH 186
PY G LY+T E+ + ++ +++P + ++VEC D+K +W+ +R R DK+ N+
Sbjct: 281 PY--GELYLTDEE--WDNLKALEEP----LEERVVECIKDDKK-RWRYLRFRDDKTNANY 331
Query: 187 ISTAYSVLESIKHPVTIGHVYNA 255
I+T V++SI PV+ ++ +A
Sbjct: 332 ITTVEKVIDSIDDPVSEKNLLDA 354
>sp|Q7SB53|MCE1_NEUCR mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 402
Score = 42.7 bits (99), Expect = 3e-04
Identities = 20/49 (40%), Positives = 32/49 (65%)
Frame = +1
Query: 88 DKYDGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVT 234
D ++VEC LD ++ +W++ R R DK++ NH ST SV+ SI+ V+
Sbjct: 322 DPLQDRVVECCLDAEN-RWRLFRFRDDKNEANHTSTVSSVMASIRDGVS 369
>sp|Q01159|MCE1_YEAST mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 459
Score = 42.0 bits (97), Expect = 4e-04
Identities = 19/47 (40%), Positives = 29/47 (61%)
Frame = +1
Query: 97 DGKIVECTLDNKSLKWKIMRERTDKSKPNHISTAYSVLESIKHPVTI 237
+G+IVEC + ++ W+++R R DK NH S VLESI V++
Sbjct: 348 NGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSVSL 394
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,891,190
Number of Sequences: 369166
Number of extensions: 958784
Number of successful extensions: 2715
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2656
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2710
length of database: 68,354,980
effective HSP length: 89
effective length of database: 51,913,565
effective search space used: 1713147645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)