Planaria EST Database


DrC_03018

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_03018
         (888 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...    76   1e-13
sp|Q03497|STE20_YEAST  Serine/threonine-protein kinase STE20       75   3e-13
sp|Q9P286|PAK7_HUMAN  Serine/threonine-protein kinase PAK 7 ...    74   7e-13
sp|Q8C015|PAK7_MOUSE  Serine/threonine-protein kinase PAK 7 ...    74   7e-13
sp|P52304|POLO_DROME  Serine/threonine-protein kinase polo         73   9e-13
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...    73   1e-12
sp|P62205|PLK1_XENTR  Serine/threonine-protein kinase PLK1 (...    72   2e-12
sp|P70032|PLK1_XENLA  Serine/threonine-protein kinase PLK1 (...    72   2e-12
sp|Q9VXE5|PAKM_DROME  Serine/threonine-protein kinase PAK mb...    72   3e-12
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...    72   3e-12
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score = 76.3 bits (186), Expect = 1e-13
 Identities = 43/108 (39%), Positives = 63/108 (58%), Gaps = 1/108 (0%)
 Frame = +2

Query: 11  LANDEMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVI 187
           LA    A S+ GT Y++APE+I +  +++  DIWSVG TVI M+TG  P+    +E+A I
Sbjct: 224 LATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAI 283

Query: 188 FKIGATRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELI 331
           F IG T K    +P  IS++    +    + EPN R TA +LL +  +
Sbjct: 284 FHIGTT-KSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330

 Score = 58.5 bits (140), Expect = 2e-08
 Identities = 30/92 (32%), Positives = 53/92 (57%)
 Frame = +2

Query: 611 EENVKKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNK 790
           +E  +   +  E+++   +  ++H NIV+Y G VR  E + I +EF+ G S+   ++  K
Sbjct: 108 KEKTQAHIQELEEEVKLLKN-LSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLE--K 164

Query: 791 FKPLPLTTIKQNTKQILKGLKFLHEQNIIHRD 886
           F   P + ++  T Q+L GL++LH   I+HRD
Sbjct: 165 FGAFPESVVRTYTNQLLLGLEYLHNHAIMHRD 196
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
          Length = 939

 Score = 74.7 bits (182), Expect = 3e-13
 Identities = 35/104 (33%), Positives = 61/104 (58%)
 Frame = +2

Query: 32   SSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRK 211
            +++ GT Y++APE+++RK Y   VDIWS+G  +I M+ G PPY     +  ++ I     
Sbjct: 772  TTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGT 831

Query: 212  LEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLS 343
             + K P  +S+ ++K +D     EP  R++A +LL+ E I  ++
Sbjct: 832  PKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIA 875

 Score = 35.4 bits (80), Expect = 0.21
 Identities = 19/69 (27%), Positives = 35/69 (50%)
 Frame = +2

Query: 680 HENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKFL 859
           H NIV +  +      +++ ME+++G SL   +         +  + + T   L GL+FL
Sbjct: 674 HPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRET---LSGLEFL 730

Query: 860 HEQNIIHRD 886
           H + ++HRD
Sbjct: 731 HSKGVLHRD 739
>sp|Q9P286|PAK7_HUMAN Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
           (PAK-7) (PAK-5)
          Length = 719

 Score = 73.6 bits (179), Expect = 7e-13
 Identities = 37/100 (37%), Positives = 60/100 (60%)
 Frame = +2

Query: 35  SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
           SL GT Y++APE+I+R PY   VDIWS+G  VI M+ G PPY     +  + +I  +   
Sbjct: 602 SLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPP 661

Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
             K   ++S+ ++  +D M   EP++R+TA +LL +  ++
Sbjct: 662 RVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLK 701

 Score = 42.4 bits (98), Expect = 0.002
 Identities = 18/70 (25%), Positives = 39/70 (55%)
 Frame = +2

Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
           +H+N+V  + +    +++++ MEF++G +L   +   +     + T+      +L+ L +
Sbjct: 502 HHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATV---CLSVLRALSY 558

Query: 857 LHEQNIIHRD 886
           LH Q +IHRD
Sbjct: 559 LHNQGVIHRD 568
>sp|Q8C015|PAK7_MOUSE Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
           (PAK-7)
          Length = 719

 Score = 73.6 bits (179), Expect = 7e-13
 Identities = 37/100 (37%), Positives = 60/100 (60%)
 Frame = +2

Query: 35  SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
           SL GT Y++APE+I+R PY   VDIWS+G  VI M+ G PPY     +  + +I  +   
Sbjct: 602 SLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPP 661

Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
             K   ++S+ ++  +D M   EP++R+TA +LL +  ++
Sbjct: 662 RVKDLHKVSSMLRGFLDLMLVREPSQRATAQELLGHPFLK 701

 Score = 43.5 bits (101), Expect = 8e-04
 Identities = 19/70 (27%), Positives = 39/70 (55%)
 Frame = +2

Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
           +H+N+V  + +    +++++ MEF++G +L   +   +     + T+      +LK L +
Sbjct: 502 HHDNVVDMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATV---CLSVLKALSY 558

Query: 857 LHEQNIIHRD 886
           LH Q +IHRD
Sbjct: 559 LHNQGVIHRD 568
>sp|P52304|POLO_DROME Serine/threonine-protein kinase polo
          Length = 576

 Score = 73.2 bits (178), Expect = 9e-13
 Identities = 35/108 (32%), Positives = 57/108 (52%)
 Frame = +2

Query: 23  EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
           E   +L GT  Y+APEI+ +K +++ VDIWS+G  +  +L G PP+          KI  
Sbjct: 178 ERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKI-- 235

Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSI 346
            +K EY++P  +      ++  M +P P  R    QLLN+E ++   +
Sbjct: 236 -KKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKV 282

 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 28/88 (31%), Positives = 46/88 (52%)
 Frame = +2

Query: 623 KKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPL 802
           +KE    E  I+R    +NH NIVK+     + + I+I +E     S+ +  K+ K   +
Sbjct: 66  QKEKTAQEITIHRS---LNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRK--SI 120

Query: 803 PLTTIKQNTKQILKGLKFLHEQNIIHRD 886
                +    QI++G+K+LH+  IIHRD
Sbjct: 121 TEFECRYYIYQIIQGVKYLHDNRIIHRD 148
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score = 72.8 bits (177), Expect = 1e-12
 Identities = 40/100 (40%), Positives = 62/100 (62%), Gaps = 3/100 (3%)
 Frame = +2

Query: 44   GTSYYLAPEIINRKPYNY--PVDIWSVGATVIHMLTGHPPYHWMQE-MAVIFKIGATRKL 214
            GT  Y+APEII++ P  Y    DIWS+G T+I M TG PP++ + E  A +FK+G   K+
Sbjct: 841  GTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMF-KV 899

Query: 215  EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
              ++P  +S E +  I    +P+P+KR+ A+ LL  E ++
Sbjct: 900  HPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLK 939

 Score = 55.8 bits (133), Expect = 2e-07
 Identities = 33/73 (45%), Positives = 46/73 (63%), Gaps = 2/73 (2%)
 Frame = +2

Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLT--TIKQNTKQILKG 847
           + H+NIV+Y G+      I IFME + G SL   + ++K+ PL     TI   TKQIL+G
Sbjct: 732 LKHKNIVQYLGSFSENGFIKIFMEQVPGGSL-SALLRSKWGPLKDNEQTIGFYTKQILEG 790

Query: 848 LKFLHEQNIIHRD 886
           LK+LH+  I+HRD
Sbjct: 791 LKYLHDNQIVHRD 803
>sp|P62205|PLK1_XENTR Serine/threonine-protein kinase PLK1 (Polo-like kinase 1) (PLK-1)
           (Plx1)
          Length = 598

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 42/144 (29%), Positives = 68/144 (47%), Gaps = 3/144 (2%)
 Frame = +2

Query: 23  EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
           E   +L GT  Y+APE++ +K +++ VDIWS+G  +  +L G PP+          +I  
Sbjct: 197 ERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRI-- 254

Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSIDYENDNSIIDQP 382
            +K EY +P+ I+     LI  M + EP  R T D LLN E      I      + +  P
Sbjct: 255 -KKNEYSVPKHINPMATALIQKMLRSEPTSRPTIDDLLNDEFFTTGYIPSRLPTTCLTVP 313

Query: 383 TK---CPDLINNAIENEKIGLSMG 445
            +    P  I+ ++      ++ G
Sbjct: 314 PRFSIAPSTIDQSLRKPLTAINKG 337

 Score = 37.0 bits (84), Expect = 0.074
 Identities = 18/71 (25%), Positives = 36/71 (50%)
 Frame = +2

Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLK 853
           ++H ++V + G   + + +++ +E     SL +  K+ K    P    +   KQ + G +
Sbjct: 99  LDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEP--EARYYLKQTILGCQ 156

Query: 854 FLHEQNIIHRD 886
           +LH   +IHRD
Sbjct: 157 YLHSNRVIHRD 167
>sp|P70032|PLK1_XENLA Serine/threonine-protein kinase PLK1 (Polo-like kinase 1) (PLK-1)
           (Plx1)
          Length = 598

 Score = 72.0 bits (175), Expect = 2e-12
 Identities = 41/144 (28%), Positives = 69/144 (47%), Gaps = 3/144 (2%)
 Frame = +2

Query: 23  EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
           E   +L GT  Y+APE++ +K +++ VDIWS+G  +  +L G PP+          +I  
Sbjct: 197 ERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRI-- 254

Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSIDYENDNSIIDQP 382
            +K EY +P+ I+     LI  M + +P  R T D LLN E   +  I      + +  P
Sbjct: 255 -KKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVP 313

Query: 383 TK---CPDLINNAIENEKIGLSMG 445
            +    P  I+ ++      ++ G
Sbjct: 314 PRFSIAPSTIDQSLRKPLTAINKG 337

 Score = 37.7 bits (86), Expect = 0.043
 Identities = 18/71 (25%), Positives = 36/71 (50%)
 Frame = +2

Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLK 853
           ++H ++V + G   + + +++ +E     SL +  K+ K    P    +   KQ + G +
Sbjct: 99  LDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEP--EARYYLKQTISGCQ 156

Query: 854 FLHEQNIIHRD 886
           +LH   +IHRD
Sbjct: 157 YLHSNRVIHRD 167
>sp|Q9VXE5|PAKM_DROME Serine/threonine-protein kinase PAK mbt (p21-activated kinase
           related protein) (Mushroom bodies tiny protein)
          Length = 639

 Score = 71.6 bits (174), Expect = 3e-12
 Identities = 36/100 (36%), Positives = 58/100 (58%)
 Frame = +2

Query: 35  SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
           SL GT Y+++PE+I+R PY   VDIWS+G  VI M+ G PP+     +  + +I   +  
Sbjct: 521 SLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPP 580

Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
             K   ++S  +Q  +D M   +P +R+TA +LL +  +R
Sbjct: 581 NLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLR 620

 Score = 45.1 bits (105), Expect = 3e-04
 Identities = 21/70 (30%), Positives = 41/70 (58%)
 Frame = +2

Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
           +H NIV+ + +    +++++ ME+++G +L   +  ++     + T+    KQ LK L +
Sbjct: 421 HHPNIVETYSSFLVNDELWVVMEYLEGGALTDIVTHSRMDEEQIATV---CKQCLKALAY 477

Query: 857 LHEQNIIHRD 886
           LH Q +IHRD
Sbjct: 478 LHSQGVIHRD 487
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score = 71.6 bits (174), Expect = 3e-12
 Identities = 41/108 (37%), Positives = 63/108 (58%), Gaps = 1/108 (0%)
 Frame = +2

Query: 11  LANDEMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVI 187
           LA    A S+ GT Y++APE+I +  +++  DIWSVG TVI M+TG  P+    +E+A I
Sbjct: 225 LATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAI 284

Query: 188 FKIGATRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELI 331
           F IG T K    +P  +S++ +  +    +  PN R TA +LL +  +
Sbjct: 285 FFIGTT-KSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 31/101 (30%), Positives = 58/101 (57%)
 Frame = +2

Query: 584 VMKIIEYVDEENVKKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGES 763
           V+    +  +E  +   +  E+++   +  ++H NIV+Y G VR  + + I +EF+ G S
Sbjct: 100 VLIAANFASKEKTQAHIQELEEEVKLLKN-LSHPNIVRYLGTVREDDTLNILLEFVPGGS 158

Query: 764 LFQKIKKNKFKPLPLTTIKQNTKQILKGLKFLHEQNIIHRD 886
           +   ++K  F P P + ++  T+Q+L GL++LH   I+HRD
Sbjct: 159 ISSLLEK--FGPFPESVVRTYTRQLLLGLEYLHNHAIMHRD 197
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,061,517
Number of Sequences: 369166
Number of extensions: 1832091
Number of successful extensions: 8148
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7690
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)