Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_E13
(888 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 76 1e-13
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 75 3e-13
sp|Q9P286|PAK7_HUMAN Serine/threonine-protein kinase PAK 7 ... 74 7e-13
sp|Q8C015|PAK7_MOUSE Serine/threonine-protein kinase PAK 7 ... 74 7e-13
sp|P52304|POLO_DROME Serine/threonine-protein kinase polo 73 9e-13
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 73 1e-12
sp|P62205|PLK1_XENTR Serine/threonine-protein kinase PLK1 (... 72 2e-12
sp|P70032|PLK1_XENLA Serine/threonine-protein kinase PLK1 (... 72 2e-12
sp|Q9VXE5|PAKM_DROME Serine/threonine-protein kinase PAK mb... 72 3e-12
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 72 3e-12
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 76.3 bits (186), Expect = 1e-13
Identities = 43/108 (39%), Positives = 63/108 (58%), Gaps = 1/108 (0%)
Frame = +2
Query: 11 LANDEMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVI 187
LA A S+ GT Y++APE+I + +++ DIWSVG TVI M+TG P+ +E+A I
Sbjct: 224 LATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAI 283
Query: 188 FKIGATRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELI 331
F IG T K +P IS++ + + EPN R TA +LL + +
Sbjct: 284 FHIGTT-KSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
Score = 58.5 bits (140), Expect = 2e-08
Identities = 30/92 (32%), Positives = 53/92 (57%)
Frame = +2
Query: 611 EENVKKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNK 790
+E + + E+++ + ++H NIV+Y G VR E + I +EF+ G S+ ++ K
Sbjct: 108 KEKTQAHIQELEEEVKLLKN-LSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLE--K 164
Query: 791 FKPLPLTTIKQNTKQILKGLKFLHEQNIIHRD 886
F P + ++ T Q+L GL++LH I+HRD
Sbjct: 165 FGAFPESVVRTYTNQLLLGLEYLHNHAIMHRD 196
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 74.7 bits (182), Expect = 3e-13
Identities = 35/104 (33%), Positives = 61/104 (58%)
Frame = +2
Query: 32 SSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRK 211
+++ GT Y++APE+++RK Y VDIWS+G +I M+ G PPY + ++ I
Sbjct: 772 TTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGT 831
Query: 212 LEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLS 343
+ K P +S+ ++K +D EP R++A +LL+ E I ++
Sbjct: 832 PKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIA 875
Score = 35.4 bits (80), Expect = 0.21
Identities = 19/69 (27%), Positives = 35/69 (50%)
Frame = +2
Query: 680 HENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKFL 859
H NIV + + +++ ME+++G SL + + + + T L GL+FL
Sbjct: 674 HPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRET---LSGLEFL 730
Query: 860 HEQNIIHRD 886
H + ++HRD
Sbjct: 731 HSKGVLHRD 739
>sp|Q9P286|PAK7_HUMAN Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
(PAK-7) (PAK-5)
Length = 719
Score = 73.6 bits (179), Expect = 7e-13
Identities = 37/100 (37%), Positives = 60/100 (60%)
Frame = +2
Query: 35 SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
SL GT Y++APE+I+R PY VDIWS+G VI M+ G PPY + + +I +
Sbjct: 602 SLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPP 661
Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
K ++S+ ++ +D M EP++R+TA +LL + ++
Sbjct: 662 RVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLK 701
Score = 42.4 bits (98), Expect = 0.002
Identities = 18/70 (25%), Positives = 39/70 (55%)
Frame = +2
Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
+H+N+V + + +++++ MEF++G +L + + + T+ +L+ L +
Sbjct: 502 HHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATV---CLSVLRALSY 558
Query: 857 LHEQNIIHRD 886
LH Q +IHRD
Sbjct: 559 LHNQGVIHRD 568
>sp|Q8C015|PAK7_MOUSE Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
(PAK-7)
Length = 719
Score = 73.6 bits (179), Expect = 7e-13
Identities = 37/100 (37%), Positives = 60/100 (60%)
Frame = +2
Query: 35 SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
SL GT Y++APE+I+R PY VDIWS+G VI M+ G PPY + + +I +
Sbjct: 602 SLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPP 661
Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
K ++S+ ++ +D M EP++R+TA +LL + ++
Sbjct: 662 RVKDLHKVSSMLRGFLDLMLVREPSQRATAQELLGHPFLK 701
Score = 43.5 bits (101), Expect = 8e-04
Identities = 19/70 (27%), Positives = 39/70 (55%)
Frame = +2
Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
+H+N+V + + +++++ MEF++G +L + + + T+ +LK L +
Sbjct: 502 HHDNVVDMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATV---CLSVLKALSY 558
Query: 857 LHEQNIIHRD 886
LH Q +IHRD
Sbjct: 559 LHNQGVIHRD 568
>sp|P52304|POLO_DROME Serine/threonine-protein kinase polo
Length = 576
Score = 73.2 bits (178), Expect = 9e-13
Identities = 35/108 (32%), Positives = 57/108 (52%)
Frame = +2
Query: 23 EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
E +L GT Y+APEI+ +K +++ VDIWS+G + +L G PP+ KI
Sbjct: 178 ERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKI-- 235
Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSI 346
+K EY++P + ++ M +P P R QLLN+E ++ +
Sbjct: 236 -KKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKV 282
Score = 45.8 bits (107), Expect = 2e-04
Identities = 28/88 (31%), Positives = 46/88 (52%)
Frame = +2
Query: 623 KKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPL 802
+KE E I+R +NH NIVK+ + + I+I +E S+ + K+ K +
Sbjct: 66 QKEKTAQEITIHRS---LNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRK--SI 120
Query: 803 PLTTIKQNTKQILKGLKFLHEQNIIHRD 886
+ QI++G+K+LH+ IIHRD
Sbjct: 121 TEFECRYYIYQIIQGVKYLHDNRIIHRD 148
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 72.8 bits (177), Expect = 1e-12
Identities = 40/100 (40%), Positives = 62/100 (62%), Gaps = 3/100 (3%)
Frame = +2
Query: 44 GTSYYLAPEIINRKPYNY--PVDIWSVGATVIHMLTGHPPYHWMQE-MAVIFKIGATRKL 214
GT Y+APEII++ P Y DIWS+G T+I M TG PP++ + E A +FK+G K+
Sbjct: 841 GTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMF-KV 899
Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
++P +S E + I +P+P+KR+ A+ LL E ++
Sbjct: 900 HPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLK 939
Score = 55.8 bits (133), Expect = 2e-07
Identities = 33/73 (45%), Positives = 46/73 (63%), Gaps = 2/73 (2%)
Frame = +2
Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLT--TIKQNTKQILKG 847
+ H+NIV+Y G+ I IFME + G SL + ++K+ PL TI TKQIL+G
Sbjct: 732 LKHKNIVQYLGSFSENGFIKIFMEQVPGGSL-SALLRSKWGPLKDNEQTIGFYTKQILEG 790
Query: 848 LKFLHEQNIIHRD 886
LK+LH+ I+HRD
Sbjct: 791 LKYLHDNQIVHRD 803
>sp|P62205|PLK1_XENTR Serine/threonine-protein kinase PLK1 (Polo-like kinase 1) (PLK-1)
(Plx1)
Length = 598
Score = 72.4 bits (176), Expect = 2e-12
Identities = 42/144 (29%), Positives = 68/144 (47%), Gaps = 3/144 (2%)
Frame = +2
Query: 23 EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
E +L GT Y+APE++ +K +++ VDIWS+G + +L G PP+ +I
Sbjct: 197 ERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRI-- 254
Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSIDYENDNSIIDQP 382
+K EY +P+ I+ LI M + EP R T D LLN E I + + P
Sbjct: 255 -KKNEYSVPKHINPMATALIQKMLRSEPTSRPTIDDLLNDEFFTTGYIPSRLPTTCLTVP 313
Query: 383 TK---CPDLINNAIENEKIGLSMG 445
+ P I+ ++ ++ G
Sbjct: 314 PRFSIAPSTIDQSLRKPLTAINKG 337
Score = 37.0 bits (84), Expect = 0.074
Identities = 18/71 (25%), Positives = 36/71 (50%)
Frame = +2
Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLK 853
++H ++V + G + + +++ +E SL + K+ K P + KQ + G +
Sbjct: 99 LDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEP--EARYYLKQTILGCQ 156
Query: 854 FLHEQNIIHRD 886
+LH +IHRD
Sbjct: 157 YLHSNRVIHRD 167
>sp|P70032|PLK1_XENLA Serine/threonine-protein kinase PLK1 (Polo-like kinase 1) (PLK-1)
(Plx1)
Length = 598
Score = 72.0 bits (175), Expect = 2e-12
Identities = 41/144 (28%), Positives = 69/144 (47%), Gaps = 3/144 (2%)
Frame = +2
Query: 23 EMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGA 202
E +L GT Y+APE++ +K +++ VDIWS+G + +L G PP+ +I
Sbjct: 197 ERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRI-- 254
Query: 203 TRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIRNLSIDYENDNSIIDQP 382
+K EY +P+ I+ LI M + +P R T D LLN E + I + + P
Sbjct: 255 -KKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVP 313
Query: 383 TK---CPDLINNAIENEKIGLSMG 445
+ P I+ ++ ++ G
Sbjct: 314 PRFSIAPSTIDQSLRKPLTAINKG 337
Score = 37.7 bits (86), Expect = 0.043
Identities = 18/71 (25%), Positives = 36/71 (50%)
Frame = +2
Query: 674 INHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLK 853
++H ++V + G + + +++ +E SL + K+ K P + KQ + G +
Sbjct: 99 LDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEP--EARYYLKQTISGCQ 156
Query: 854 FLHEQNIIHRD 886
+LH +IHRD
Sbjct: 157 YLHSNRVIHRD 167
>sp|Q9VXE5|PAKM_DROME Serine/threonine-protein kinase PAK mbt (p21-activated kinase
related protein) (Mushroom bodies tiny protein)
Length = 639
Score = 71.6 bits (174), Expect = 3e-12
Identities = 36/100 (36%), Positives = 58/100 (58%)
Frame = +2
Query: 35 SLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPYHWMQEMAVIFKIGATRKL 214
SL GT Y+++PE+I+R PY VDIWS+G VI M+ G PP+ + + +I +
Sbjct: 521 SLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPP 580
Query: 215 EYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELIR 334
K ++S +Q +D M +P +R+TA +LL + +R
Sbjct: 581 NLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLR 620
Score = 45.1 bits (105), Expect = 3e-04
Identities = 21/70 (30%), Positives = 41/70 (58%)
Frame = +2
Query: 677 NHENIVKYFGAVRNLEKIFIFMEFIDGESLFQKIKKNKFKPLPLTTIKQNTKQILKGLKF 856
+H NIV+ + + +++++ ME+++G +L + ++ + T+ KQ LK L +
Sbjct: 421 HHPNIVETYSSFLVNDELWVVMEYLEGGALTDIVTHSRMDEEQIATV---CKQCLKALAY 477
Query: 857 LHEQNIIHRD 886
LH Q +IHRD
Sbjct: 478 LHSQGVIHRD 487
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 71.6 bits (174), Expect = 3e-12
Identities = 41/108 (37%), Positives = 63/108 (58%), Gaps = 1/108 (0%)
Frame = +2
Query: 11 LANDEMASSLWGTSYYLAPEIINRKPYNYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVI 187
LA A S+ GT Y++APE+I + +++ DIWSVG TVI M+TG P+ +E+A I
Sbjct: 225 LATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAI 284
Query: 188 FKIGATRKLEYKLPRQISNEMQKLIDDMTKPEPNKRSTADQLLNYELI 331
F IG T K +P +S++ + + + PN R TA +LL + +
Sbjct: 285 FFIGTT-KSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331
Score = 62.8 bits (151), Expect = 1e-09
Identities = 31/101 (30%), Positives = 58/101 (57%)
Frame = +2
Query: 584 VMKIIEYVDEENVKKEAKCFEDDINRYREFINHENIVKYFGAVRNLEKIFIFMEFIDGES 763
V+ + +E + + E+++ + ++H NIV+Y G VR + + I +EF+ G S
Sbjct: 100 VLIAANFASKEKTQAHIQELEEEVKLLKN-LSHPNIVRYLGTVREDDTLNILLEFVPGGS 158
Query: 764 LFQKIKKNKFKPLPLTTIKQNTKQILKGLKFLHEQNIIHRD 886
+ ++K F P P + ++ T+Q+L GL++LH I+HRD
Sbjct: 159 ISSLLEK--FGPFPESVVRTYTRQLLLGLEYLHNHAIMHRD 197
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,061,517
Number of Sequences: 369166
Number of extensions: 1832091
Number of successful extensions: 8148
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7690
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)