Planaria EST Database


DrC_02987

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02987
         (567 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...    55   9e-08
sp|Q8T0S6|HIPPO_DROME  Serine/threonine-protein kinase hippo       55   9e-08
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...    55   1e-07
sp|P52304|POLO_DROME  Serine/threonine-protein kinase polo         54   2e-07
sp|Q03497|STE20_YEAST  Serine/threonine-protein kinase STE20       54   3e-07
sp|Q9JI10|STK3_MOUSE  Serine/threonine-protein kinase 3 (STE...    53   6e-07
sp|Q13188|STK3_HUMAN  Serine/threonine-protein kinase 3 (STE...    53   6e-07
sp|O22042|M3K3_ARATH  Mitogen-activated protein kinase kinas...    53   6e-07
sp|Q13043|STK4_HUMAN  Serine/threonine-protein kinase 4 (STE...    52   1e-06
sp|Q7RAH3|CDPK1_PLAYO  Calcium-dependent protein kinase 1          52   1e-06
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score = 55.5 bits (132), Expect = 9e-08
 Identities = 32/81 (39%), Positives = 45/81 (55%), Gaps = 1/81 (1%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDD 184
           ++  DIWSVG TVI M+TG  P+    +E+A I  IGTT K    +P  IS++    +  
Sbjct: 251 SFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTT-KSHPPIPDNISSDANDFLLK 309

Query: 185 MTNPEPNKRATADQLLNYELI 247
               EPN R TA +LL +  +
Sbjct: 310 CLQQEPNLRPTASELLKHPFV 330
>sp|Q8T0S6|HIPPO_DROME Serine/threonine-protein kinase hippo
          Length = 669

 Score = 55.5 bits (132), Expect = 9e-08
 Identities = 30/83 (36%), Positives = 41/83 (49%)
 Frame = +2

Query: 20  DIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNPE 199
           DIWS+G T + M  G PPY  +  M  I  I       ++ P + S E    +      E
Sbjct: 218 DIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKE 277

Query: 200 PNKRATADQLLNYELIRNLPNHS 268
           P+ RATA +LL +E IRN  + S
Sbjct: 278 PDDRATATELLEHEFIRNAKHRS 300
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 30/78 (38%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
 Frame = +2

Query: 20   DIWSVGATVIHMLTGHPPYHWMQE-MAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNP 196
            DIWS+G T+I M TG PP++ + E  A + K+G   K+  ++P  +S E +  I     P
Sbjct: 863  DIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMF-KVHPEIPESMSAEAKAFILKCFEP 921

Query: 197  EPNKRATADQLLNYELIR 250
            +P+KRA A+ LL  E ++
Sbjct: 922  DPDKRACANDLLVDEFLK 939
>sp|P52304|POLO_DROME Serine/threonine-protein kinase polo
          Length = 576

 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 26/81 (32%), Positives = 41/81 (50%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
           ++ VDIWS+G  +  +L G PP+         SKI   +K EY++P  +      ++  M
Sbjct: 201 SFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKI---KKCEYRVPSYLRKPAADMVIAM 257

Query: 188 TNPEPNKRATADQLLNYELIR 250
             P P  R    QLLN+E ++
Sbjct: 258 LQPNPESRPAIGQLLNFEFLK 278
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
          Length = 939

 Score = 53.9 bits (128), Expect = 3e-07
 Identities = 31/89 (34%), Positives = 47/89 (52%)
 Frame = +2

Query: 17   VDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNP 196
            VDIWS+G  +I M+ G PPY     +  +  I T    + K P  +S+ ++K +D     
Sbjct: 795  VDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCV 854

Query: 197  EPNKRATADQLLNYELIRNLPNHSEINKS 283
            EP  RA+A +LL+ E I  +   +E N S
Sbjct: 855  EPEDRASATELLHDEYITEI---AEANSS 880
>sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
           (Mammalian STE20-like protein kinase 2)
          Length = 497

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 29/82 (35%), Positives = 39/82 (47%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
           N   DIWS+G T I M  G PPY  +  M  I  I T     ++ P   S++    +   
Sbjct: 199 NCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKC 258

Query: 188 TNPEPNKRATADQLLNYELIRN 253
               P +RATA QLL +  I+N
Sbjct: 259 LVKSPEQRATATQLLQHPFIKN 280
>sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
           (Mammalian STE20-like protein kinase 2)
           (Serine/threonine-protein kinase Krs-1)
          Length = 491

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 29/82 (35%), Positives = 39/82 (47%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
           N   DIWS+G T I M  G PPY  +  M  I  I T     ++ P   S++    +   
Sbjct: 199 NCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKC 258

Query: 188 TNPEPNKRATADQLLNYELIRN 253
               P +RATA QLL +  I+N
Sbjct: 259 LVKNPEQRATATQLLQHPFIKN 280
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
           NPK1-related protein kinase 3)
          Length = 651

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 29/81 (35%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDD 184
           ++  DIWSVG TVI M TG PP+    Q+ A +  IG T K    +P  +S E +  +  
Sbjct: 251 SFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRT-KAHPPIPEDLSPEAKDFLMK 309

Query: 185 MTNPEPNKRATADQLLNYELI 247
             + EP+ R +A +LL +  +
Sbjct: 310 CLHKEPSLRLSATELLQHPFV 330

 Score = 32.7 bits (73), Expect = 0.64
 Identities = 27/71 (38%), Positives = 36/71 (50%), Gaps = 5/71 (7%)
 Frame = +2

Query: 344 INSAINNHKIGLSMK----LKCPDGCEHMFCLSSVTACTFKKGEQIGNGAFGKVYK-INK 508
           INS+I + +IGL  K    L  P   E         +  ++KGE IG GAFG+VY  +N 
Sbjct: 36  INSSIRSSRIGLFSKPPPGLPAPRKEE-------APSIRWRKGELIGCGAFGRVYMGMNL 88

Query: 509 SSTPELVIKTI 541
            S   L IK +
Sbjct: 89  DSGELLAIKQV 99
>sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE20-like kinase MST1) (MST-1)
           (Mammalian STE20-like protein kinase 1)
           (Serine/threonine-protein kinase Krs-2)
          Length = 487

 Score = 52.0 bits (123), Expect = 1e-06
 Identities = 28/82 (34%), Positives = 38/82 (46%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
           N   DIWS+G T I M  G PPY  +  M  I  I T     ++ P   S+     +   
Sbjct: 202 NCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQC 261

Query: 188 TNPEPNKRATADQLLNYELIRN 253
               P +RATA QLL +  +R+
Sbjct: 262 LVKSPEQRATATQLLQHPFVRS 283
>sp|Q7RAH3|CDPK1_PLAYO Calcium-dependent protein kinase 1
          Length = 535

 Score = 51.6 bits (122), Expect = 1e-06
 Identities = 33/97 (34%), Positives = 52/97 (53%), Gaps = 4/97 (4%)
 Frame = +2

Query: 8   NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLP----RQISNEMQKL 175
           N   D+WS G  +  +L G+PP+    +  +I K+   EK +Y       + IS+E ++L
Sbjct: 244 NEKCDVWSCGVIMYILLCGYPPFGGQNDQDIIKKV---EKGKYYFDFNDWKNISDEAKEL 300

Query: 176 IDDMTNPEPNKRATADQLLNYELIRNLPNHSEINKSN 286
           I  M   + NKR TA++ LN   I+   N+  INKS+
Sbjct: 301 IKLMLTYDYNKRCTAEEALNSRWIKKYANN--INKSD 335
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,489,133
Number of Sequences: 369166
Number of extensions: 1285029
Number of successful extensions: 5480
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4883
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5423
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4127973360
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)