Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_N21
(567 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 55 9e-08
sp|Q8T0S6|HIPPO_DROME Serine/threonine-protein kinase hippo 55 9e-08
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 55 1e-07
sp|P52304|POLO_DROME Serine/threonine-protein kinase polo 54 2e-07
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 54 3e-07
sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE... 53 6e-07
sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE... 53 6e-07
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 53 6e-07
sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE... 52 1e-06
sp|Q7RAH3|CDPK1_PLAYO Calcium-dependent protein kinase 1 52 1e-06
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 55.5 bits (132), Expect = 9e-08
Identities = 32/81 (39%), Positives = 45/81 (55%), Gaps = 1/81 (1%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDD 184
++ DIWSVG TVI M+TG P+ +E+A I IGTT K +P IS++ +
Sbjct: 251 SFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTT-KSHPPIPDNISSDANDFLLK 309
Query: 185 MTNPEPNKRATADQLLNYELI 247
EPN R TA +LL + +
Sbjct: 310 CLQQEPNLRPTASELLKHPFV 330
>sp|Q8T0S6|HIPPO_DROME Serine/threonine-protein kinase hippo
Length = 669
Score = 55.5 bits (132), Expect = 9e-08
Identities = 30/83 (36%), Positives = 41/83 (49%)
Frame = +2
Query: 20 DIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNPE 199
DIWS+G T + M G PPY + M I I ++ P + S E + E
Sbjct: 218 DIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKE 277
Query: 200 PNKRATADQLLNYELIRNLPNHS 268
P+ RATA +LL +E IRN + S
Sbjct: 278 PDDRATATELLEHEFIRNAKHRS 300
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 55.1 bits (131), Expect = 1e-07
Identities = 30/78 (38%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +2
Query: 20 DIWSVGATVIHMLTGHPPYHWMQE-MAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNP 196
DIWS+G T+I M TG PP++ + E A + K+G K+ ++P +S E + I P
Sbjct: 863 DIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMF-KVHPEIPESMSAEAKAFILKCFEP 921
Query: 197 EPNKRATADQLLNYELIR 250
+P+KRA A+ LL E ++
Sbjct: 922 DPDKRACANDLLVDEFLK 939
>sp|P52304|POLO_DROME Serine/threonine-protein kinase polo
Length = 576
Score = 54.3 bits (129), Expect = 2e-07
Identities = 26/81 (32%), Positives = 41/81 (50%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
++ VDIWS+G + +L G PP+ SKI +K EY++P + ++ M
Sbjct: 201 SFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKI---KKCEYRVPSYLRKPAADMVIAM 257
Query: 188 TNPEPNKRATADQLLNYELIR 250
P P R QLLN+E ++
Sbjct: 258 LQPNPESRPAIGQLLNFEFLK 278
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 53.9 bits (128), Expect = 3e-07
Identities = 31/89 (34%), Positives = 47/89 (52%)
Frame = +2
Query: 17 VDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDMTNP 196
VDIWS+G +I M+ G PPY + + I T + K P +S+ ++K +D
Sbjct: 795 VDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCV 854
Query: 197 EPNKRATADQLLNYELIRNLPNHSEINKS 283
EP RA+A +LL+ E I + +E N S
Sbjct: 855 EPEDRASATELLHDEYITEI---AEANSS 880
>sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
Length = 497
Score = 52.8 bits (125), Expect = 6e-07
Identities = 29/82 (35%), Positives = 39/82 (47%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
N DIWS+G T I M G PPY + M I I T ++ P S++ +
Sbjct: 199 NCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKC 258
Query: 188 TNPEPNKRATADQLLNYELIRN 253
P +RATA QLL + I+N
Sbjct: 259 LVKSPEQRATATQLLQHPFIKN 280
>sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
(Serine/threonine-protein kinase Krs-1)
Length = 491
Score = 52.8 bits (125), Expect = 6e-07
Identities = 29/82 (35%), Positives = 39/82 (47%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
N DIWS+G T I M G PPY + M I I T ++ P S++ +
Sbjct: 199 NCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKC 258
Query: 188 TNPEPNKRATADQLLNYELIRN 253
P +RATA QLL + I+N
Sbjct: 259 LVKNPEQRATATQLLQHPFIKN 280
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 52.8 bits (125), Expect = 6e-07
Identities = 29/81 (35%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPY-HWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDD 184
++ DIWSVG TVI M TG PP+ Q+ A + IG T K +P +S E + +
Sbjct: 251 SFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRT-KAHPPIPEDLSPEAKDFLMK 309
Query: 185 MTNPEPNKRATADQLLNYELI 247
+ EP+ R +A +LL + +
Sbjct: 310 CLHKEPSLRLSATELLQHPFV 330
Score = 32.7 bits (73), Expect = 0.64
Identities = 27/71 (38%), Positives = 36/71 (50%), Gaps = 5/71 (7%)
Frame = +2
Query: 344 INSAINNHKIGLSMK----LKCPDGCEHMFCLSSVTACTFKKGEQIGNGAFGKVYK-INK 508
INS+I + +IGL K L P E + ++KGE IG GAFG+VY +N
Sbjct: 36 INSSIRSSRIGLFSKPPPGLPAPRKEE-------APSIRWRKGELIGCGAFGRVYMGMNL 88
Query: 509 SSTPELVIKTI 541
S L IK +
Sbjct: 89 DSGELLAIKQV 99
>sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE20-like kinase MST1) (MST-1)
(Mammalian STE20-like protein kinase 1)
(Serine/threonine-protein kinase Krs-2)
Length = 487
Score = 52.0 bits (123), Expect = 1e-06
Identities = 28/82 (34%), Positives = 38/82 (46%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLPRQISNEMQKLIDDM 187
N DIWS+G T I M G PPY + M I I T ++ P S+ +
Sbjct: 202 NCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQC 261
Query: 188 TNPEPNKRATADQLLNYELIRN 253
P +RATA QLL + +R+
Sbjct: 262 LVKSPEQRATATQLLQHPFVRS 283
>sp|Q7RAH3|CDPK1_PLAYO Calcium-dependent protein kinase 1
Length = 535
Score = 51.6 bits (122), Expect = 1e-06
Identities = 33/97 (34%), Positives = 52/97 (53%), Gaps = 4/97 (4%)
Frame = +2
Query: 8 NYPVDIWSVGATVIHMLTGHPPYHWMQEMAVISKIGTTEKLEYKLP----RQISNEMQKL 175
N D+WS G + +L G+PP+ + +I K+ EK +Y + IS+E ++L
Sbjct: 244 NEKCDVWSCGVIMYILLCGYPPFGGQNDQDIIKKV---EKGKYYFDFNDWKNISDEAKEL 300
Query: 176 IDDMTNPEPNKRATADQLLNYELIRNLPNHSEINKSN 286
I M + NKR TA++ LN I+ N+ INKS+
Sbjct: 301 IKLMLTYDYNKRCTAEEALNSRWIKKYANN--INKSD 335
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,489,133
Number of Sequences: 369166
Number of extensions: 1285029
Number of successful extensions: 5480
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4883
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5423
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4127973360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)