Planaria EST Database


DrC_02889

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02889
         (891 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O76463|NFT1_CAEEL  Nitrilase and fragile histidine triad ...   271   2e-72
sp|O76464|NFT1_DROME  Nitrilase and fragile histidine triad ...   251   2e-66
sp|Q8VDK1|NIT1_MOUSE  Nitrilase homolog 1                         216   5e-56
sp|Q86X76|NIT1_HUMAN  Nitrilase homolog 1                         215   2e-55
sp|Q7TQ94|NIT1_RAT  Nitrilase homolog 1                           213   4e-55
sp|P47016|NIT2_YEAST  Probable hydrolase NIT2                     187   3e-47
sp|P55175|Y601_SYNY3  Hypothetical UPF0012 protein sll0601        154   4e-37
sp|Q10166|YAUB_SCHPO  Hypothetical UPF0012 protein C26A3.11 ...   148   2e-35
sp|P49954|NIT3_YEAST  Probable hydrolase NIT3                     144   3e-34
sp|P55177|YAG5_STAAU  Hypothetical UPF0012 protein in agr op...   103   6e-22
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein NitFhit
           [Includes: Bis(5'-adenosyl)-triphosphatase (Diadenosine
           5',5'''-P1,P3-triphosphate hydrolase)
           (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
           Nitrilase homolog ]
          Length = 440

 Score =  271 bits (692), Expect = 2e-72
 Identities = 135/287 (47%), Positives = 183/287 (63%)
 Frame = +2

Query: 29  FNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLGLMGMSI 208
           +NTH++ID+ GV    Y+KLHLFD++IP    + ES   +AG  +  PV+TP+G +G+SI
Sbjct: 109 WNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSI 168

Query: 209 CYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQEGRH 388
           CYDVRFPELS   R   A +L+FPSAFT  TG AHW  LLRARA++NQC+VVAAAQ G H
Sbjct: 169 CYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAH 228

Query: 389 NSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRRIDLFQP 568
           N KR SYGHS+VVDPWGA++A+ +E    +   ++DL  +D +R   PVF HRR DL+  
Sbjct: 229 NPKRQSYGHSMVVDPWGAVVAQCSE-RVDMCFAEIDLSYVDTLREMQPVFSHRRSDLY-- 285

Query: 569 NARRIKAINIRKEAFQFGPHSVESSCVFLQSTEAFAFVNISPLVPGHVLVSPIRIVPRFA 748
               I   +      +F   ++ +  +F  +  +F FVN+ P+  GHVLVSP R+VPR  
Sbjct: 286 -TLHINEKSSETGGLKFARFNIPADHIFYSTPHSFVFVNLKPVTDGHVLVSPKRVVPRLT 344

Query: 749 DLTLTELTQIYFLVHAIGPRLTAHFGASAMNISQQDGPDAGQSVPHV 889
           DLT  E   ++ +   +   L  H   ++  I  QDG DAGQ+VPHV
Sbjct: 345 DLTDAETADLFIVAKKVQAMLEKHHNVTSTTICVQDGKDAGQTVPHV 391
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein NitFhit (NFT-1
           protein) [Includes: Bis(5'-adenosyl)-triphosphatase
           (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
           (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
           Nitrilase homolog ]
          Length = 460

 Score =  251 bits (641), Expect = 2e-66
 Identities = 127/299 (42%), Positives = 184/299 (61%), Gaps = 3/299 (1%)
 Frame = +2

Query: 2   ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
           +   N+QK FN HV+++ KG +   Y KLH+FDV    +  +RES  V  G C++ PV T
Sbjct: 115 VHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTKEVR-LRESDTVTPGYCLERPVST 173

Query: 182 PLGLMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFV 361
           P+G +G+ ICYD+RF E +  LR   AN+LT+PSAFT  TG+AHW +LLRARA++ QCFV
Sbjct: 174 PVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYATGKAHWEILLRARAIETQCFV 233

Query: 362 VAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQ 541
           VAAAQ G HN KR S+GHS++V PWG ++A+ +E    +   +VDL V+  +   +P F+
Sbjct: 234 VAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEVDLSVLQSLYQTMPCFE 293

Query: 542 HRRIDLFQPNARRIKAINIRKEAFQ---FGPHSVESSCVFLQSTEAFAFVNISPLVPGHV 712
           HRR D++   A  +++    KE  Q   F  + V+   +F +S   FAF N+  +V GHV
Sbjct: 294 HRRNDIYALTAYNLRS----KEPTQDRPFATNIVDKRTIFYESEHCFAFTNLRCVVKGHV 349

Query: 713 LVSPIRIVPRFADLTLTELTQIYFLVHAIGPRLTAHFGASAMNISQQDGPDAGQSVPHV 889
           LVS  R+ PR   L   E+  ++  V  +   L   +  ++  ++ QDG  AGQ+VPHV
Sbjct: 350 LVSTKRVTPRLCGLDCAEMADMFTTVCLVQRLLEKIYQTTSATVTVQDGAQAGQTVPHV 408
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1
          Length = 323

 Score =  216 bits (551), Expect = 5e-56
 Identities = 100/184 (54%), Positives = 138/184 (75%)
 Frame = +2

Query: 11  ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
           E NQK +N HV++++KG +V +Y K HL DV+IP    +RES+  + GG ++ PV+TP G
Sbjct: 133 EQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTKPGGTLEPPVKTPAG 192

Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
            +G++ICYD+RFPELS  L  + A +LT+ SAF + TG AHW VLLRARA+++QC+V+AA
Sbjct: 193 KVGLAICYDMRFPELSLKLAQAGAEILTYSSAFGSVTGPAHWEVLLRARAIESQCYVIAA 252

Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
           AQ GRH+  RASYGHS+VVDPWG ++A R   GPGL +  +DL  + ++R +LPVFQHRR
Sbjct: 253 AQCGRHHETRASYGHSMVVDPWGTVVA-RCSEGPGLCLARIDLHFLQQMRQHLPVFQHRR 311

Query: 551 IDLF 562
            DL+
Sbjct: 312 PDLY 315
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1
          Length = 327

 Score =  215 bits (547), Expect = 2e-55
 Identities = 100/184 (54%), Positives = 135/184 (73%)
 Frame = +2

Query: 11  ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
           E  QK +N HV++++KG +V TY K HL DV+IP    + ES+    G  ++ PV TP G
Sbjct: 137 EQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAG 196

Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
            +G+++CYD+RFPELS +L  + A +LT+PSAF + TG AHW VLLRARA++ QC+VVAA
Sbjct: 197 KIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAA 256

Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
           AQ GRH+ KRASYGHS+VVDPWG ++A R   GPGL +  +DL  + ++R +LPVFQHRR
Sbjct: 257 AQCGRHHEKRASYGHSMVVDPWGTVVA-RCSEGPGLCLARIDLNYLRQLRRHLPVFQHRR 315

Query: 551 IDLF 562
            DL+
Sbjct: 316 PDLY 319
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1
          Length = 292

 Score =  213 bits (543), Expect = 4e-55
 Identities = 99/184 (53%), Positives = 137/184 (74%)
 Frame = +2

Query: 11  ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
           E  QK +N HV++++KG +V +Y K HL DV+IP    +RES+    G  ++ PV+TP G
Sbjct: 102 EQTQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTMPGYALEPPVKTPAG 161

Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
            +G++ICYD+RFPELS  L  + A +LT+PSAF + TG AHW VLLRARA+++QC+V+AA
Sbjct: 162 KVGLAICYDMRFPELSLKLAQAGAEILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAA 221

Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
           AQ GRH+  RASYGHS+VVDPWG ++A  +E GPGL +  +DL  + ++R +LPVFQHRR
Sbjct: 222 AQCGRHHETRASYGHSMVVDPWGTVVASCSE-GPGLCLARIDLHFLQQMRQHLPVFQHRR 280

Query: 551 IDLF 562
            DL+
Sbjct: 281 PDLY 284
>sp|P47016|NIT2_YEAST Probable hydrolase NIT2
          Length = 307

 Score =  187 bits (475), Expect = 3e-47
 Identities = 95/205 (46%), Positives = 132/205 (64%), Gaps = 21/205 (10%)
 Frame = +2

Query: 11  ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
           E N +  N  + ID++G I+  Y KLHLFDVD+PN   ++ES  VQ G  + + +E+PLG
Sbjct: 103 EGNDRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIPDIIESPLG 162

Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
            +G +ICYD+RFPE S  LR+  A +L FPSAFT +TGEAHW +L RARAVD QC+V+  
Sbjct: 163 KLGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIKTGEAHWELLGRARAVDTQCYVLMP 222

Query: 371 AQEGRH------------------NSKRASYGHSLVVDPWGAIIAERAEP---GPGLLMC 487
            Q G H                  +S+R S+GHS+V+DPWG IIA  A+P   GP L++ 
Sbjct: 223 GQVGMHDLSDPEWEKQSHMSALEKSSRRESWGHSMVIDPWGKIIA-HADPSTVGPQLILA 281

Query: 488 DVDLGVIDKIRSNLPVFQHRRIDLF 562
           D+D  ++ +IR+ +P++  RR DLF
Sbjct: 282 DLDRELLQEIRNKMPLWNQRRDDLF 306
>sp|P55175|Y601_SYNY3 Hypothetical UPF0012 protein sll0601
          Length = 272

 Score =  154 bits (388), Expect = 4e-37
 Identities = 75/176 (42%), Positives = 113/176 (64%), Gaps = 1/176 (0%)
 Frame = +2

Query: 23  KTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETP-LGLMG 199
           K +NT  +I   G  +  Y K+HLFDV++P+  +  ES+ V AG        +   G +G
Sbjct: 95  KAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESATVMAGQKYPPVYHSDSFGNLG 154

Query: 200 MSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQE 379
           +SICYDVRFPEL + L    A+VL  P+AFT  TG+ HW VLL+ARA++N C+V+A AQ 
Sbjct: 155 LSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQVLLQARAIENTCYVIAPAQT 214

Query: 380 GRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHR 547
           G H  +R ++GH++++DPWG I+A+  E  PGL + +++   + ++R  +P  QHR
Sbjct: 215 GCHYERRHTHGHAMIIDPWGVILADAGEK-PGLAIAEINPDRLKQVRQQMPSLQHR 269
>sp|Q10166|YAUB_SCHPO Hypothetical UPF0012 protein C26A3.11 in chromosome I
          Length = 322

 Score =  148 bits (374), Expect = 2e-35
 Identities = 74/187 (39%), Positives = 109/187 (58%)
 Frame = +2

Query: 2   ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
           I    + K +NT +V D  G ++  + K+HLFD+DIP   S RES  +  G  +   V+T
Sbjct: 127 IPERKDGKLYNTAMVFDPSGKLIAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTM-VDT 185

Query: 182 PLGLMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFV 361
             G  G+ ICYD+RFPEL+     +  +V+ +P AF   TG  HW +L RARAVDN+ FV
Sbjct: 186 EYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFV 245

Query: 362 VAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQ 541
              A     N+   S+GHS VVDP+G +IA   E  P ++  D+D  V+   R+++P++ 
Sbjct: 246 ACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEK-PSIVYADIDPSVMSTARNSVPIYT 304

Query: 542 HRRIDLF 562
            RR D++
Sbjct: 305 QRRFDVY 311
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3
          Length = 291

 Score =  144 bits (363), Expect = 3e-34
 Identities = 73/180 (40%), Positives = 107/180 (59%)
 Frame = +2

Query: 23  KTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLGLMGM 202
           K +NT ++ +  G ++  + K+HLFDVDIPN  S  ES  +  G      ++T  G  G+
Sbjct: 108 KIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISFHESETLSPGE-KSTTIDTKYGKFGV 166

Query: 203 SICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQEG 382
            ICYD+RFPEL+       A  + +PSAF T TG  HWH+L R+RAVDNQ +V+  +   
Sbjct: 167 GICYDMRFPELAMLSARKGAFAMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPAR 226

Query: 383 RHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRRIDLF 562
              S   +YGHS+VVDP G I+AE  E G  ++  ++D  VI+  R  +P+ + RR D++
Sbjct: 227 NLQSSYHAYGHSIVVDPRGKIVAEAGE-GEEIIYAELDPEVIESFRQAVPLTKQRRFDVY 285
>sp|P55177|YAG5_STAAU Hypothetical UPF0012 protein in agr operon (ORF 5)
          Length = 261

 Score =  103 bits (257), Expect = 6e-22
 Identities = 61/189 (32%), Positives = 103/189 (54%), Gaps = 1/189 (0%)
 Frame = +2

Query: 2   ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
           +SN  N + FNT   ++  G ++  YDK+HL  +       +RE   + AG  V EP + 
Sbjct: 83  VSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPM-------LREHEFLTAGEYVAEPFQL 135

Query: 182 PLGL-MGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCF 358
             G  +   ICYD+RFPEL +    S A +  + + +     + HWH LL+ARA++N  F
Sbjct: 136 SDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQ-HWHSLLKARAIENNMF 194

Query: 359 VVAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVF 538
           V+     G   +   + GHS+V++P G ++ E  E    +L  D++L  +++ R N+PVF
Sbjct: 195 VIGTNSTGFDGNTEYA-GHSIVINPNGDLVGELNE-SADILTVDLNLNEVEQQRENIPVF 252

Query: 539 QHRRIDLFQ 565
           +  ++DL++
Sbjct: 253 KSIKLDLYK 261
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,404,652
Number of Sequences: 369166
Number of extensions: 2276256
Number of successful extensions: 8124
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8061
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)