Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_J08
(891 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad ... 271 2e-72
sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad ... 251 2e-66
sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 216 5e-56
sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 215 2e-55
sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 213 4e-55
sp|P47016|NIT2_YEAST Probable hydrolase NIT2 187 3e-47
sp|P55175|Y601_SYNY3 Hypothetical UPF0012 protein sll0601 154 4e-37
sp|Q10166|YAUB_SCHPO Hypothetical UPF0012 protein C26A3.11 ... 148 2e-35
sp|P49954|NIT3_YEAST Probable hydrolase NIT3 144 3e-34
sp|P55177|YAG5_STAAU Hypothetical UPF0012 protein in agr op... 103 6e-22
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein NitFhit
[Includes: Bis(5'-adenosyl)-triphosphatase (Diadenosine
5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog ]
Length = 440
Score = 271 bits (692), Expect = 2e-72
Identities = 135/287 (47%), Positives = 183/287 (63%)
Frame = +2
Query: 29 FNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLGLMGMSI 208
+NTH++ID+ GV Y+KLHLFD++IP + ES +AG + PV+TP+G +G+SI
Sbjct: 109 WNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSI 168
Query: 209 CYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQEGRH 388
CYDVRFPELS R A +L+FPSAFT TG AHW LLRARA++NQC+VVAAAQ G H
Sbjct: 169 CYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAH 228
Query: 389 NSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRRIDLFQP 568
N KR SYGHS+VVDPWGA++A+ +E + ++DL +D +R PVF HRR DL+
Sbjct: 229 NPKRQSYGHSMVVDPWGAVVAQCSE-RVDMCFAEIDLSYVDTLREMQPVFSHRRSDLY-- 285
Query: 569 NARRIKAINIRKEAFQFGPHSVESSCVFLQSTEAFAFVNISPLVPGHVLVSPIRIVPRFA 748
I + +F ++ + +F + +F FVN+ P+ GHVLVSP R+VPR
Sbjct: 286 -TLHINEKSSETGGLKFARFNIPADHIFYSTPHSFVFVNLKPVTDGHVLVSPKRVVPRLT 344
Query: 749 DLTLTELTQIYFLVHAIGPRLTAHFGASAMNISQQDGPDAGQSVPHV 889
DLT E ++ + + L H ++ I QDG DAGQ+VPHV
Sbjct: 345 DLTDAETADLFIVAKKVQAMLEKHHNVTSTTICVQDGKDAGQTVPHV 391
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein NitFhit (NFT-1
protein) [Includes: Bis(5'-adenosyl)-triphosphatase
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog ]
Length = 460
Score = 251 bits (641), Expect = 2e-66
Identities = 127/299 (42%), Positives = 184/299 (61%), Gaps = 3/299 (1%)
Frame = +2
Query: 2 ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
+ N+QK FN HV+++ KG + Y KLH+FDV + +RES V G C++ PV T
Sbjct: 115 VHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTKEVR-LRESDTVTPGYCLERPVST 173
Query: 182 PLGLMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFV 361
P+G +G+ ICYD+RF E + LR AN+LT+PSAFT TG+AHW +LLRARA++ QCFV
Sbjct: 174 PVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYATGKAHWEILLRARAIETQCFV 233
Query: 362 VAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQ 541
VAAAQ G HN KR S+GHS++V PWG ++A+ +E + +VDL V+ + +P F+
Sbjct: 234 VAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEVDLSVLQSLYQTMPCFE 293
Query: 542 HRRIDLFQPNARRIKAINIRKEAFQ---FGPHSVESSCVFLQSTEAFAFVNISPLVPGHV 712
HRR D++ A +++ KE Q F + V+ +F +S FAF N+ +V GHV
Sbjct: 294 HRRNDIYALTAYNLRS----KEPTQDRPFATNIVDKRTIFYESEHCFAFTNLRCVVKGHV 349
Query: 713 LVSPIRIVPRFADLTLTELTQIYFLVHAIGPRLTAHFGASAMNISQQDGPDAGQSVPHV 889
LVS R+ PR L E+ ++ V + L + ++ ++ QDG AGQ+VPHV
Sbjct: 350 LVSTKRVTPRLCGLDCAEMADMFTTVCLVQRLLEKIYQTTSATVTVQDGAQAGQTVPHV 408
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1
Length = 323
Score = 216 bits (551), Expect = 5e-56
Identities = 100/184 (54%), Positives = 138/184 (75%)
Frame = +2
Query: 11 ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
E NQK +N HV++++KG +V +Y K HL DV+IP +RES+ + GG ++ PV+TP G
Sbjct: 133 EQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTKPGGTLEPPVKTPAG 192
Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
+G++ICYD+RFPELS L + A +LT+ SAF + TG AHW VLLRARA+++QC+V+AA
Sbjct: 193 KVGLAICYDMRFPELSLKLAQAGAEILTYSSAFGSVTGPAHWEVLLRARAIESQCYVIAA 252
Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
AQ GRH+ RASYGHS+VVDPWG ++A R GPGL + +DL + ++R +LPVFQHRR
Sbjct: 253 AQCGRHHETRASYGHSMVVDPWGTVVA-RCSEGPGLCLARIDLHFLQQMRQHLPVFQHRR 311
Query: 551 IDLF 562
DL+
Sbjct: 312 PDLY 315
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1
Length = 327
Score = 215 bits (547), Expect = 2e-55
Identities = 100/184 (54%), Positives = 135/184 (73%)
Frame = +2
Query: 11 ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
E QK +N HV++++KG +V TY K HL DV+IP + ES+ G ++ PV TP G
Sbjct: 137 EQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAG 196
Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
+G+++CYD+RFPELS +L + A +LT+PSAF + TG AHW VLLRARA++ QC+VVAA
Sbjct: 197 KIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAA 256
Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
AQ GRH+ KRASYGHS+VVDPWG ++A R GPGL + +DL + ++R +LPVFQHRR
Sbjct: 257 AQCGRHHEKRASYGHSMVVDPWGTVVA-RCSEGPGLCLARIDLNYLRQLRRHLPVFQHRR 315
Query: 551 IDLF 562
DL+
Sbjct: 316 PDLY 319
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1
Length = 292
Score = 213 bits (543), Expect = 4e-55
Identities = 99/184 (53%), Positives = 137/184 (74%)
Frame = +2
Query: 11 ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
E QK +N HV++++KG +V +Y K HL DV+IP +RES+ G ++ PV+TP G
Sbjct: 102 EQTQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTMPGYALEPPVKTPAG 161
Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
+G++ICYD+RFPELS L + A +LT+PSAF + TG AHW VLLRARA+++QC+V+AA
Sbjct: 162 KVGLAICYDMRFPELSLKLAQAGAEILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAA 221
Query: 371 AQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRR 550
AQ GRH+ RASYGHS+VVDPWG ++A +E GPGL + +DL + ++R +LPVFQHRR
Sbjct: 222 AQCGRHHETRASYGHSMVVDPWGTVVASCSE-GPGLCLARIDLHFLQQMRQHLPVFQHRR 280
Query: 551 IDLF 562
DL+
Sbjct: 281 PDLY 284
>sp|P47016|NIT2_YEAST Probable hydrolase NIT2
Length = 307
Score = 187 bits (475), Expect = 3e-47
Identities = 95/205 (46%), Positives = 132/205 (64%), Gaps = 21/205 (10%)
Frame = +2
Query: 11 ENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLG 190
E N + N + ID++G I+ Y KLHLFDVD+PN ++ES VQ G + + +E+PLG
Sbjct: 103 EGNDRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIPDIIESPLG 162
Query: 191 LMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAA 370
+G +ICYD+RFPE S LR+ A +L FPSAFT +TGEAHW +L RARAVD QC+V+
Sbjct: 163 KLGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIKTGEAHWELLGRARAVDTQCYVLMP 222
Query: 371 AQEGRH------------------NSKRASYGHSLVVDPWGAIIAERAEP---GPGLLMC 487
Q G H +S+R S+GHS+V+DPWG IIA A+P GP L++
Sbjct: 223 GQVGMHDLSDPEWEKQSHMSALEKSSRRESWGHSMVIDPWGKIIA-HADPSTVGPQLILA 281
Query: 488 DVDLGVIDKIRSNLPVFQHRRIDLF 562
D+D ++ +IR+ +P++ RR DLF
Sbjct: 282 DLDRELLQEIRNKMPLWNQRRDDLF 306
>sp|P55175|Y601_SYNY3 Hypothetical UPF0012 protein sll0601
Length = 272
Score = 154 bits (388), Expect = 4e-37
Identities = 75/176 (42%), Positives = 113/176 (64%), Gaps = 1/176 (0%)
Frame = +2
Query: 23 KTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETP-LGLMG 199
K +NT +I G + Y K+HLFDV++P+ + ES+ V AG + G +G
Sbjct: 95 KAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESATVMAGQKYPPVYHSDSFGNLG 154
Query: 200 MSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQE 379
+SICYDVRFPEL + L A+VL P+AFT TG+ HW VLL+ARA++N C+V+A AQ
Sbjct: 155 LSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQVLLQARAIENTCYVIAPAQT 214
Query: 380 GRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHR 547
G H +R ++GH++++DPWG I+A+ E PGL + +++ + ++R +P QHR
Sbjct: 215 GCHYERRHTHGHAMIIDPWGVILADAGEK-PGLAIAEINPDRLKQVRQQMPSLQHR 269
>sp|Q10166|YAUB_SCHPO Hypothetical UPF0012 protein C26A3.11 in chromosome I
Length = 322
Score = 148 bits (374), Expect = 2e-35
Identities = 74/187 (39%), Positives = 109/187 (58%)
Frame = +2
Query: 2 ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
I + K +NT +V D G ++ + K+HLFD+DIP S RES + G + V+T
Sbjct: 127 IPERKDGKLYNTAMVFDPSGKLIAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTM-VDT 185
Query: 182 PLGLMGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFV 361
G G+ ICYD+RFPEL+ + +V+ +P AF TG HW +L RARAVDN+ FV
Sbjct: 186 EYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFV 245
Query: 362 VAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQ 541
A N+ S+GHS VVDP+G +IA E P ++ D+D V+ R+++P++
Sbjct: 246 ACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEK-PSIVYADIDPSVMSTARNSVPIYT 304
Query: 542 HRRIDLF 562
RR D++
Sbjct: 305 QRRFDVY 311
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3
Length = 291
Score = 144 bits (363), Expect = 3e-34
Identities = 73/180 (40%), Positives = 107/180 (59%)
Frame = +2
Query: 23 KTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVETPLGLMGM 202
K +NT ++ + G ++ + K+HLFDVDIPN S ES + G ++T G G+
Sbjct: 108 KIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISFHESETLSPGE-KSTTIDTKYGKFGV 166
Query: 203 SICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCFVVAAAQEG 382
ICYD+RFPEL+ A + +PSAF T TG HWH+L R+RAVDNQ +V+ +
Sbjct: 167 GICYDMRFPELAMLSARKGAFAMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPAR 226
Query: 383 RHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVFQHRRIDLF 562
S +YGHS+VVDP G I+AE E G ++ ++D VI+ R +P+ + RR D++
Sbjct: 227 NLQSSYHAYGHSIVVDPRGKIVAEAGE-GEEIIYAELDPEVIESFRQAVPLTKQRRFDVY 285
>sp|P55177|YAG5_STAAU Hypothetical UPF0012 protein in agr operon (ORF 5)
Length = 261
Score = 103 bits (257), Expect = 6e-22
Identities = 61/189 (32%), Positives = 103/189 (54%), Gaps = 1/189 (0%)
Frame = +2
Query: 2 ISNENNQKTFNTHVVIDNKGVIVGTYDKLHLFDVDIPNLESIRESSVVQAGGCVKEPVET 181
+SN N + FNT ++ G ++ YDK+HL + +RE + AG V EP +
Sbjct: 83 VSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPM-------LREHEFLTAGEYVAEPFQL 135
Query: 182 PLGL-MGMSICYDVRFPELSQSLRASSANVLTFPSAFTTRTGEAHWHVLLRARAVDNQCF 358
G + ICYD+RFPEL + S A + + + + + HWH LL+ARA++N F
Sbjct: 136 SDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQ-HWHSLLKARAIENNMF 194
Query: 359 VVAAAQEGRHNSKRASYGHSLVVDPWGAIIAERAEPGPGLLMCDVDLGVIDKIRSNLPVF 538
V+ G + + GHS+V++P G ++ E E +L D++L +++ R N+PVF
Sbjct: 195 VIGTNSTGFDGNTEYA-GHSIVINPNGDLVGELNE-SADILTVDLNLNEVEQQRENIPVF 252
Query: 539 QHRRIDLFQ 565
+ ++DL++
Sbjct: 253 KSIKLDLYK 261
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,404,652
Number of Sequences: 369166
Number of extensions: 2276256
Number of successful extensions: 8124
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8061
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)