Planaria EST Database


DrC_02587

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02587
         (345 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O60573|IF4E3_HUMAN  Eukaryotic translation initiation fac...    97   9e-21
sp|Q8BMB3|IF4E3_MOUSE  Eukaryotic translation initiation fac...    97   9e-21
sp|Q22888|IF4E4_CAEEL  Eukaryotic translation initiation fac...    91   1e-18
sp|Q9FK59|IFE3_ARATH  Eukaryotic translation initiation fact...    79   4e-15
sp|O04663|IF4E2_ARATH  Eukaryotic translation initiation fac...    55   4e-08
sp|P48598|IF4E_DROME  Eukaryotic translation initiation fact...    55   4e-08
sp|O81481|IF4E1_MAIZE  Eukaryotic translation initiation fac...    55   7e-08
sp|P48599|IF4E1_ORYSA  Eukaryotic translation initiation fac...    54   9e-08
sp|O23252|IF4E1_ARATH  Eukaryotic translation initiation fac...    54   9e-08
sp|P06730|IF4E_HUMAN  Eukaryotic translation initiation fact...    54   1e-07
>sp|O60573|IF4E3_HUMAN Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
           (eIF-4E type 3) (mRNA cap-binding protein type 3)
           (Eukaryotic translation initiation factor 4E-like 3)
           (Eukaryotic translation initiation factor 4E homologous
           protein) (mRNA cap-binding protein 4EHP) (eIF4E-like
           protein 4E-LP)
          Length = 245

 Score = 97.4 bits (241), Expect = 9e-21
 Identities = 45/73 (61%), Positives = 58/73 (79%)
 Frame = +2

Query: 14  NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           NL+LAML ++F V +EICG VVSVR QEDI+S+WNK+  ++A   RIR+ + RVL LPPN
Sbjct: 150 NLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPN 209

Query: 194 TVIEYKNHADSIK 232
           T++EYK H DSIK
Sbjct: 210 TIMEYKTHTDSIK 222
>sp|Q8BMB3|IF4E3_MOUSE Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
           (eIF-4E type 3) (mRNA cap-binding protein type 3)
           (Eukaryotic translation initiation factor 4E-like 3)
           (eIF4E-like protein 4E-LP)
          Length = 245

 Score = 97.4 bits (241), Expect = 9e-21
 Identities = 45/73 (61%), Positives = 58/73 (79%)
 Frame = +2

Query: 14  NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           NL+LAML ++F V +EICG VVSVR QEDI+S+WNK+  ++A   RIR+ + RVL LPPN
Sbjct: 150 NLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPN 209

Query: 194 TVIEYKNHADSIK 232
           T++EYK H DSIK
Sbjct: 210 TIMEYKTHTDSIK 222
>sp|Q22888|IF4E4_CAEEL Eukaryotic translation initiation factor 4E-4 (eIF4E-4) (eIF-4E-4)
           (mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
          Length = 212

 Score = 90.5 bits (223), Expect = 1e-18
 Identities = 40/81 (49%), Positives = 60/81 (74%)
 Frame = +2

Query: 14  NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           NL++A++ ++F V DE+CG V S+R QEDI+S+WN++ D+     RIRE +  VL+LP N
Sbjct: 126 NLLMAIIGEQFLVGDELCGAVCSIRNQEDIISLWNRNADDTPVTNRIRETLRSVLQLPQN 185

Query: 194 TVIEYKNHADSIKDSSSFYNT 256
           TV+EYK H D ++D SS+ +T
Sbjct: 186 TVLEYKRHDDCLRDQSSYRHT 206
>sp|Q9FK59|IFE3_ARATH Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
           (eIF-4E type 3) (mRNA cap-binding protein type 3) (Novel
           cap-binding protein) (nCBP)
          Length = 221

 Score = 78.6 bits (192), Expect = 4e-15
 Identities = 38/81 (46%), Positives = 58/81 (71%)
 Frame = +2

Query: 14  NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           +L+LA++ D+ D  D ICG V+SVR  EDI+SVWN++  +  A M +R+++ R L+LP  
Sbjct: 137 DLLLALVGDQLDDADNICGAVLSVRFNEDIISVWNRNASDHQAVMGLRDSIKRHLKLPHA 196

Query: 194 TVIEYKNHADSIKDSSSFYNT 256
            V+EYK H  S++D+SS+ NT
Sbjct: 197 YVMEYKPHDASLRDNSSYRNT 217
>sp|O04663|IF4E2_ARATH Eukaryotic translation initiation factor 4E-2 (eIF4E-2) (eIF-4E-2)
           (mRNA cap-binding protein) (eIF-(iso)4F 25 kDa subunit)
           (eIF-(iso)4F p28 subunit) (eIF4Eiso protein)
           (eIF(iso)4E)
          Length = 198

 Score = 55.5 bits (132), Expect = 4e-08
 Identities = 30/71 (42%), Positives = 45/71 (63%), Gaps = 2/71 (2%)
 Frame = +2

Query: 20  VLAMLTDKFDVEDEICGVVVSVRGQ--EDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           ++A++ ++FD  DEICGVV SVR Q  +D LS+W ++K NEA  M I +    +L++   
Sbjct: 123 LMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDK 182

Query: 194 TVIEYKNHADS 226
             I + NH DS
Sbjct: 183 --ITFNNHDDS 191
>sp|P48598|IF4E_DROME Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
           cap-binding protein) (eIF-4F 25 kDa subunit)
          Length = 259

 Score = 55.5 bits (132), Expect = 4e-08
 Identities = 24/82 (29%), Positives = 47/82 (57%)
 Frame = +2

Query: 14  NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
           +++L ++ + FD  D+ICG V+++RG+ + +S+W    +NE A + I   +   L L  N
Sbjct: 175 DVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRN 234

Query: 194 TVIEYKNHADSIKDSSSFYNTI 259
             ++Y+ H D++    S   +I
Sbjct: 235 NSLQYQLHKDTMVKQGSNVKSI 256
>sp|O81481|IF4E1_MAIZE Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
           (mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
           (eIF-4F p26 subunit)
          Length = 218

 Score = 54.7 bits (130), Expect = 7e-08
 Identities = 24/54 (44%), Positives = 40/54 (74%)
 Frame = +2

Query: 20  VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLE 181
           +LAM+ ++FD  DEICG VVSVRG+++ +++W K+  NEAA++ I +    +L+
Sbjct: 139 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQVSIGKQWKELLD 192
>sp|P48599|IF4E1_ORYSA Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
           (mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
           (eIF-4F p26 subunit)
          Length = 227

 Score = 54.3 bits (129), Expect = 9e-08
 Identities = 23/45 (51%), Positives = 36/45 (80%)
 Frame = +2

Query: 20  VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRI 154
           +LAM+ ++FD  DEICG VVSVRG+++ +++W K+  NEAA++ I
Sbjct: 148 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQISI 192
>sp|O23252|IF4E1_ARATH Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
           (mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
           (eIF-4F p26 subunit)
          Length = 235

 Score = 54.3 bits (129), Expect = 9e-08
 Identities = 27/71 (38%), Positives = 46/71 (64%)
 Frame = +2

Query: 20  VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPNTV 199
           +LA++ ++FD  DEICG VV++RG+++ +S+W K+  NEAA++ I +     L+   N  
Sbjct: 156 LLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDY--NNS 213

Query: 200 IEYKNHADSIK 232
           I +  H D+ K
Sbjct: 214 IGFIIHEDAKK 224
>sp|P06730|IF4E_HUMAN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
           cap-binding protein) (eIF-4F 25 kDa subunit)
          Length = 217

 Score = 53.9 bits (128), Expect = 1e-07
 Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
 Frame = +2

Query: 20  VLAMLTDKFD-VEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPNT 196
           +L ++ + FD   D++CG VV+VR + D +++W    +N  A   I       L LPP  
Sbjct: 134 LLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKI 193

Query: 197 VIEYKNHADSIKDSSS 244
           VI Y++HAD+   S S
Sbjct: 194 VIGYQSHADTATKSGS 209
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,789,258
Number of Sequences: 369166
Number of extensions: 695409
Number of successful extensions: 2420
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2343
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2416
length of database: 68,354,980
effective HSP length: 82
effective length of database: 53,206,710
effective search space used: 1702614720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)