Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_K03
(345 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O60573|IF4E3_HUMAN Eukaryotic translation initiation fac... 97 9e-21
sp|Q8BMB3|IF4E3_MOUSE Eukaryotic translation initiation fac... 97 9e-21
sp|Q22888|IF4E4_CAEEL Eukaryotic translation initiation fac... 91 1e-18
sp|Q9FK59|IFE3_ARATH Eukaryotic translation initiation fact... 79 4e-15
sp|O04663|IF4E2_ARATH Eukaryotic translation initiation fac... 55 4e-08
sp|P48598|IF4E_DROME Eukaryotic translation initiation fact... 55 4e-08
sp|O81481|IF4E1_MAIZE Eukaryotic translation initiation fac... 55 7e-08
sp|P48599|IF4E1_ORYSA Eukaryotic translation initiation fac... 54 9e-08
sp|O23252|IF4E1_ARATH Eukaryotic translation initiation fac... 54 9e-08
sp|P06730|IF4E_HUMAN Eukaryotic translation initiation fact... 54 1e-07
>sp|O60573|IF4E3_HUMAN Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
(eIF-4E type 3) (mRNA cap-binding protein type 3)
(Eukaryotic translation initiation factor 4E-like 3)
(Eukaryotic translation initiation factor 4E homologous
protein) (mRNA cap-binding protein 4EHP) (eIF4E-like
protein 4E-LP)
Length = 245
Score = 97.4 bits (241), Expect = 9e-21
Identities = 45/73 (61%), Positives = 58/73 (79%)
Frame = +2
Query: 14 NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
NL+LAML ++F V +EICG VVSVR QEDI+S+WNK+ ++A RIR+ + RVL LPPN
Sbjct: 150 NLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPN 209
Query: 194 TVIEYKNHADSIK 232
T++EYK H DSIK
Sbjct: 210 TIMEYKTHTDSIK 222
>sp|Q8BMB3|IF4E3_MOUSE Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
(eIF-4E type 3) (mRNA cap-binding protein type 3)
(Eukaryotic translation initiation factor 4E-like 3)
(eIF4E-like protein 4E-LP)
Length = 245
Score = 97.4 bits (241), Expect = 9e-21
Identities = 45/73 (61%), Positives = 58/73 (79%)
Frame = +2
Query: 14 NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
NL+LAML ++F V +EICG VVSVR QEDI+S+WNK+ ++A RIR+ + RVL LPPN
Sbjct: 150 NLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPN 209
Query: 194 TVIEYKNHADSIK 232
T++EYK H DSIK
Sbjct: 210 TIMEYKTHTDSIK 222
>sp|Q22888|IF4E4_CAEEL Eukaryotic translation initiation factor 4E-4 (eIF4E-4) (eIF-4E-4)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 212
Score = 90.5 bits (223), Expect = 1e-18
Identities = 40/81 (49%), Positives = 60/81 (74%)
Frame = +2
Query: 14 NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
NL++A++ ++F V DE+CG V S+R QEDI+S+WN++ D+ RIRE + VL+LP N
Sbjct: 126 NLLMAIIGEQFLVGDELCGAVCSIRNQEDIISLWNRNADDTPVTNRIRETLRSVLQLPQN 185
Query: 194 TVIEYKNHADSIKDSSSFYNT 256
TV+EYK H D ++D SS+ +T
Sbjct: 186 TVLEYKRHDDCLRDQSSYRHT 206
>sp|Q9FK59|IFE3_ARATH Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)
(eIF-4E type 3) (mRNA cap-binding protein type 3) (Novel
cap-binding protein) (nCBP)
Length = 221
Score = 78.6 bits (192), Expect = 4e-15
Identities = 38/81 (46%), Positives = 58/81 (71%)
Frame = +2
Query: 14 NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
+L+LA++ D+ D D ICG V+SVR EDI+SVWN++ + A M +R+++ R L+LP
Sbjct: 137 DLLLALVGDQLDDADNICGAVLSVRFNEDIISVWNRNASDHQAVMGLRDSIKRHLKLPHA 196
Query: 194 TVIEYKNHADSIKDSSSFYNT 256
V+EYK H S++D+SS+ NT
Sbjct: 197 YVMEYKPHDASLRDNSSYRNT 217
>sp|O04663|IF4E2_ARATH Eukaryotic translation initiation factor 4E-2 (eIF4E-2) (eIF-4E-2)
(mRNA cap-binding protein) (eIF-(iso)4F 25 kDa subunit)
(eIF-(iso)4F p28 subunit) (eIF4Eiso protein)
(eIF(iso)4E)
Length = 198
Score = 55.5 bits (132), Expect = 4e-08
Identities = 30/71 (42%), Positives = 45/71 (63%), Gaps = 2/71 (2%)
Frame = +2
Query: 20 VLAMLTDKFDVEDEICGVVVSVRGQ--EDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
++A++ ++FD DEICGVV SVR Q +D LS+W ++K NEA M I + +L++
Sbjct: 123 LMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDK 182
Query: 194 TVIEYKNHADS 226
I + NH DS
Sbjct: 183 --ITFNNHDDS 191
>sp|P48598|IF4E_DROME Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 259
Score = 55.5 bits (132), Expect = 4e-08
Identities = 24/82 (29%), Positives = 47/82 (57%)
Frame = +2
Query: 14 NLVLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPN 193
+++L ++ + FD D+ICG V+++RG+ + +S+W +NE A + I + L L N
Sbjct: 175 DVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRN 234
Query: 194 TVIEYKNHADSIKDSSSFYNTI 259
++Y+ H D++ S +I
Sbjct: 235 NSLQYQLHKDTMVKQGSNVKSI 256
>sp|O81481|IF4E1_MAIZE Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
(eIF-4F p26 subunit)
Length = 218
Score = 54.7 bits (130), Expect = 7e-08
Identities = 24/54 (44%), Positives = 40/54 (74%)
Frame = +2
Query: 20 VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLE 181
+LAM+ ++FD DEICG VVSVRG+++ +++W K+ NEAA++ I + +L+
Sbjct: 139 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQVSIGKQWKELLD 192
>sp|P48599|IF4E1_ORYSA Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
(eIF-4F p26 subunit)
Length = 227
Score = 54.3 bits (129), Expect = 9e-08
Identities = 23/45 (51%), Positives = 36/45 (80%)
Frame = +2
Query: 20 VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRI 154
+LAM+ ++FD DEICG VVSVRG+++ +++W K+ NEAA++ I
Sbjct: 148 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQISI 192
>sp|O23252|IF4E1_ARATH Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
(eIF-4F p26 subunit)
Length = 235
Score = 54.3 bits (129), Expect = 9e-08
Identities = 27/71 (38%), Positives = 46/71 (64%)
Frame = +2
Query: 20 VLAMLTDKFDVEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPNTV 199
+LA++ ++FD DEICG VV++RG+++ +S+W K+ NEAA++ I + L+ N
Sbjct: 156 LLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDY--NNS 213
Query: 200 IEYKNHADSIK 232
I + H D+ K
Sbjct: 214 IGFIIHEDAKK 224
>sp|P06730|IF4E_HUMAN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 53.9 bits (128), Expect = 1e-07
Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
Frame = +2
Query: 20 VLAMLTDKFD-VEDEICGVVVSVRGQEDILSVWNKSKDNEAAKMRIREAMFRVLELPPNT 196
+L ++ + FD D++CG VV+VR + D +++W +N A I L LPP
Sbjct: 134 LLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKI 193
Query: 197 VIEYKNHADSIKDSSS 244
VI Y++HAD+ S S
Sbjct: 194 VIGYQSHADTATKSGS 209
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,789,258
Number of Sequences: 369166
Number of extensions: 695409
Number of successful extensions: 2420
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2343
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2416
length of database: 68,354,980
effective HSP length: 82
effective length of database: 53,206,710
effective search space used: 1702614720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)