Planaria EST Database


DrC_02438

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02438
         (865 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P42898|MTHR_HUMAN  Methylenetetrahydrofolate reductase         272   8e-73
sp|Q9WU20|MTHR_MOUSE  Methylenetetrahydrofolate reductase         269   9e-72
sp|Q60HE5|MTHR_MACFA  Methylenetetrahydrofolate reductase         268   1e-71
sp|Q17693|MTHR_CAEEL  Probable methylenetetrahydrofolate red...   218   1e-56
sp|Q9SE60|MTHR1_ARATH  Methylenetetrahydrofolate reductase 1...   184   3e-46
sp|Q9SE94|MTHR1_MAIZE  Methylenetetrahydrofolate reductase 1...   181   2e-45
sp|Q75HE6|MTHR_ORYSA  Probable methylenetetrahydrofolate red...   176   8e-44
sp|O80585|MTHR2_ARATH  Methylenetetrahydrofolate reductase 2...   166   6e-41
sp|Q10258|MTHR1_SCHPO  Methylenetetrahydrofolate reductase 1      146   8e-35
sp|P53128|MTHR2_YEAST  Methylenetetrahydrofolate reductase 2      140   3e-33
>sp|P42898|MTHR_HUMAN Methylenetetrahydrofolate reductase
          Length = 656

 Score =  272 bits (696), Expect = 8e-73
 Identities = 130/238 (54%), Positives = 172/238 (72%)
 Frame = -1

Query: 835  LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
            LFYL+ +  KEELL MW  +L SE  V++VF  +++  PN NG  V  LPW+ +  L  E
Sbjct: 405  LFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWN-DEPLAAE 463

Query: 655  TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
            T +++ EL R+N QGILTINSQP ING  S+D I GWG   GY++QKAYLEFF      E
Sbjct: 464  TSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAE 523

Query: 475  SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
            +L++VL +Y  RVNYH+V  K  +ITN  ++   A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 524  ALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFW 583

Query: 295  KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
            K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDN++  +NCL+++V+  L+
Sbjct: 584  KDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLE 641
>sp|Q9WU20|MTHR_MOUSE Methylenetetrahydrofolate reductase
          Length = 654

 Score =  269 bits (687), Expect = 9e-72
 Identities = 126/238 (52%), Positives = 171/238 (71%)
 Frame = -1

Query: 835  LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
            LFYL+ +  +EELL MW  +L SE  V++VF H+++  PN +G+ V  LPW+ +  L  E
Sbjct: 404  LFYLKSKSpreELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWN-DEPLAAE 462

Query: 655  TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
            T +++ EL R+N  GILTINSQP IN   S+D + GWG   GY++QKAYLEFF     +E
Sbjct: 463  TSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVE 522

Query: 475  SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
            +L++VL  Y  RVNYHIV  K  +ITN  ++   A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 523  ALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFW 582

Query: 295  KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
            K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDNE+  ++CL+++V+   +
Sbjct: 583  KDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFE 640
>sp|Q60HE5|MTHR_MACFA Methylenetetrahydrofolate reductase
          Length = 656

 Score =  268 bits (685), Expect = 1e-71
 Identities = 127/238 (53%), Positives = 171/238 (71%)
 Frame = -1

Query: 835  LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
            LFYL+ +  +E LL MW  +L SE  V++VF  +++  PN NG  V  LPW+ +  L  E
Sbjct: 405  LFYLKSKSpreLLLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWN-DEPLAAE 463

Query: 655  TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
            T +++ EL R+N QGILTINSQP ING  S+D + GWG   GY++QKAYLEFF      E
Sbjct: 464  TSLLKEELLRVNRQGILTINSQPNINGKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETAE 523

Query: 475  SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
            +L++VL +Y  RVNYH+V  K  +ITN  ++   A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 524  ALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFW 583

Query: 295  KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
            K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDN++  +NCL+++V+  L+
Sbjct: 584  KDEAFALWIEQWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDALE 641
>sp|Q17693|MTHR_CAEEL Probable methylenetetrahydrofolate reductase
          Length = 663

 Score =  218 bits (556), Expect = 1e-56
 Identities = 112/232 (48%), Positives = 153/232 (65%), Gaps = 4/232 (1%)
 Frame = -1

Query: 805  EELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPW-HPNTELENETKIIQAELG 629
            ++ L M+   + S  DV +VF ++ITQ PNA+G  V  LPW    T ++ ET +I  +L 
Sbjct: 429  DDRLAMFGANIESFEDVKRVFINYITQAPNADGVKVTVLPWTEAETGVQPETSLISEQLV 488

Query: 628  RLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESLIKVLN-E 452
              NE GILT+NSQP +NG  STD + GWG P GY YQKAYLE F+     + LI+++  E
Sbjct: 489  WCNENGILTVNSQPSVNGAPSTDPLVGWGKPGGYCYQKAYLECFMTAELSDKLIQIIERE 548

Query: 451  YSPRVNYHIVQSKDG--DITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKREAFD 278
            +  RVNYH + +KD   D TN ++   IA+TWGVFPGSEI QPTVVDPL+F+ W+ EA+ 
Sbjct: 549  FPVRVNYHAI-NKDSTFDKTNSEETTPIAVTWGVFPGSEIAQPTVVDPLSFRAWRDEAYQ 607

Query: 277  LWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
            +W+  WG  Y + S S  VI+ +HD + LV LVDN++ K + LF ++QK LD
Sbjct: 608  MWMAQWGDFYPKESKSYGVIKAVHDEFRLVTLVDNDFQKPSVLFDVLQKALD 659
>sp|Q9SE60|MTHR1_ARATH Methylenetetrahydrofolate reductase 1 (AtMTHFR1)
          Length = 592

 Score =  184 bits (467), Expect = 3e-46
 Identities = 91/222 (40%), Positives = 132/222 (59%)
 Frame = -1

Query: 829  YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
            + R +G  ++L   W   L S  DV + F              +KS PW     L+ ETK
Sbjct: 370  FARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGN-------LKSSPWSELDGLQPETK 422

Query: 649  IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
            II  +LG++N  G LTINSQP +N  +S     GWG P GY+YQKAYLEFF     +++L
Sbjct: 423  IINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTL 482

Query: 469  IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
            ++    + P + Y  V   +  ++N  + +  A+TWGVFP  E+IQPT+VDP +FK WK 
Sbjct: 483  VEKSKAF-PSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKD 541

Query: 289  EAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
            EAF++W + W  LY E+  SR++++ + ++YYLV+LVDN YI
Sbjct: 542  EAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYI 583
>sp|Q9SE94|MTHR1_MAIZE Methylenetetrahydrofolate reductase 1 (ZmMTHFR1)
          Length = 593

 Score =  181 bits (459), Expect = 2e-45
 Identities = 96/224 (42%), Positives = 137/224 (61%), Gaps = 1/224 (0%)
 Frame = -1

Query: 829  YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
            + R RG  ++L + W   L S  D+ + F      T    G +  S PW     L+ ETK
Sbjct: 370  FTRPRGRGKKLQEEWAVPLKSVEDISERF------TNFCQGKLTSS-PWSELDGLQPETK 422

Query: 649  IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
            II  +L  +N++G LTINSQP +NG +S     GWG P GY+YQKAYLEFF     ++ L
Sbjct: 423  IIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEKLDQL 482

Query: 469  IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAI-AITWGVFPGSEIIQPTVVDPLAFKYWK 293
            I+ +  + P + Y I  +KDG+  +    NA+ A+TWGVFPG EIIQPTVVD  +F  WK
Sbjct: 483  IEKIKAF-PSLTY-IAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWK 540

Query: 292  REAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIK 161
             EAF++W + WG ++ E   SR++++ +   YYLV+LVDN+Y++
Sbjct: 541  DEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQ 584
>sp|Q75HE6|MTHR_ORYSA Probable methylenetetrahydrofolate reductase
          Length = 594

 Score =  176 bits (446), Expect = 8e-44
 Identities = 94/223 (42%), Positives = 134/223 (60%), Gaps = 1/223 (0%)
 Frame = -1

Query: 829  YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
            + R RG  ++L + W   + S  D+ + F +         G +  S PW     L+ ETK
Sbjct: 370  FTRPRGRGKKLQEEWAVPVKSVEDINERFMNF------CQGKLTSS-PWSELDGLQPETK 422

Query: 649  IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
            II  +L ++N++G LTINSQP +NG  S     GWG P GY+YQKAYLEFF     ++ L
Sbjct: 423  IIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEKLDQL 482

Query: 469  IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAI-AITWGVFPGSEIIQPTVVDPLAFKYWK 293
            I+    + P + Y I  +KDG+  +    NA+ A+TWGVFPG EI+QPTVVD  +F  WK
Sbjct: 483  IEKSKAF-PSLTY-IAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWK 540

Query: 292  REAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
             EAF++W K W  L+ E   SR+++  +  +Y+LV+LVDN+YI
Sbjct: 541  DEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYI 583
>sp|O80585|MTHR2_ARATH Methylenetetrahydrofolate reductase 2 (AtMTHFR2)
          Length = 594

 Score =  166 bits (421), Expect = 6e-41
 Identities = 86/222 (38%), Positives = 125/222 (56%)
 Frame = -1

Query: 829  YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
            + R R   ++L   W   L S  D+ + F              +KS PW     L+ ET+
Sbjct: 370  FSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGN-------LKSSPWSELDGLQPETR 422

Query: 649  IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
            II  +L ++N +G LTINSQP +N   S     GWG P GY+YQKAYLEFF     ++++
Sbjct: 423  IINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAV 482

Query: 469  IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
            ++      P + Y  V   +  ++N    +  A+TWGVFP  EIIQPT+VDP +F  WK 
Sbjct: 483  VEKCKAL-PSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKD 541

Query: 289  EAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
            EAF+ W + W  LY E   SR +++ + ++YYLV+LV+N+YI
Sbjct: 542  EAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYI 583
>sp|Q10258|MTHR1_SCHPO Methylenetetrahydrofolate reductase 1
          Length = 603

 Score =  146 bits (368), Expect = 8e-35
 Identities = 78/194 (40%), Positives = 119/194 (61%), Gaps = 3/194 (1%)
 Frame = -1

Query: 700 VKSLPWHPNTELENETKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIY 521
           + SLPW  +  + +E  +I+ +L  +N    LTINSQP +NG +S+  +FGWG PNGY++
Sbjct: 400 ISSLPWS-DLPISDEADLIRDQLLSMNRNAFLTINSQPALNGEKSSHPVFGWGPPNGYVF 458

Query: 520 QKAYLEFFIHHRHIESLIKVLNEYSPRVN--YHIVQSKDGDI-TNCDDVNAIAITWGVFP 350
           QK Y+EFF+H     SL+  L E   ++N   + V +K+GD+ TN       A+TWGVFP
Sbjct: 459 QKPYVEFFVH----PSLLNELKETVKKLNSVSYFVTNKNGDLDTNSQYEIPNAVTWGVFP 514

Query: 349 GSEIIQPTVVDPLAFKYWKREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNE 170
             EIIQPT+V+  +F  WK EA+ L ++ W   Y  +SISR+++  M   ++L  +VDN+
Sbjct: 515 NREIIQPTIVESTSFLAWKDEAYSLGME-WANAYSPDSISRKLLVSMMKEWFLCVIVDND 573

Query: 169 YIKENCLFKLVQKV 128
           +     LF +  K+
Sbjct: 574 FQNGQSLFDVFNKM 587
>sp|P53128|MTHR2_YEAST Methylenetetrahydrofolate reductase 2
          Length = 599

 Score =  140 bits (354), Expect = 3e-33
 Identities = 85/224 (37%), Positives = 130/224 (58%), Gaps = 5/224 (2%)
 Frame = -1

Query: 820  VRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETKIIQ 641
            +R S  + L++W     S NDV  +  +++    N N   +K LPW  +  + +E   I+
Sbjct: 376  IRQSANKCLELWSTPT-SINDVAFLVINYL----NGN---LKCLPWS-DIPINDEINPIK 426

Query: 640  AELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESLIKV 461
            A L  LN+  I+TINSQP +NG+ S D I GWG  +GY+YQK YLEF +    +  LI  
Sbjct: 427  AHLIELNQHSIITINSQPQVNGIRSNDKIHGWGPKDGYVYQKQYLEFMLPKTKLPKLIDT 486

Query: 460  L--NEYSPRVNYHIVQSKDGDITN-CDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
            L  NE+   + Y  + S+   ++N  D+  + A+TWG+FPG EI+QPT+V+ ++F  WK 
Sbjct: 487  LKNNEF---LTYFAIDSQGDLLSNHPDNSKSNAVTWGIFPGREILQPTIVEKISFLAWKE 543

Query: 289  EAFDLWIKHWGILYE--ENSISRQVIQYMHDNYYLVNLVDNEYI 164
            E + + +  W +     +   S Q IQ + D+Y LVN+VDN+YI
Sbjct: 544  EFYHI-LNEWKLNMNKYDKPHSAQFIQSLIDDYCLVNIVDNDYI 586
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,152,736
Number of Sequences: 369166
Number of extensions: 1956334
Number of successful extensions: 5125
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5110
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)