Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_D01
(865 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P42898|MTHR_HUMAN Methylenetetrahydrofolate reductase 272 8e-73
sp|Q9WU20|MTHR_MOUSE Methylenetetrahydrofolate reductase 269 9e-72
sp|Q60HE5|MTHR_MACFA Methylenetetrahydrofolate reductase 268 1e-71
sp|Q17693|MTHR_CAEEL Probable methylenetetrahydrofolate red... 218 1e-56
sp|Q9SE60|MTHR1_ARATH Methylenetetrahydrofolate reductase 1... 184 3e-46
sp|Q9SE94|MTHR1_MAIZE Methylenetetrahydrofolate reductase 1... 181 2e-45
sp|Q75HE6|MTHR_ORYSA Probable methylenetetrahydrofolate red... 176 8e-44
sp|O80585|MTHR2_ARATH Methylenetetrahydrofolate reductase 2... 166 6e-41
sp|Q10258|MTHR1_SCHPO Methylenetetrahydrofolate reductase 1 146 8e-35
sp|P53128|MTHR2_YEAST Methylenetetrahydrofolate reductase 2 140 3e-33
>sp|P42898|MTHR_HUMAN Methylenetetrahydrofolate reductase
Length = 656
Score = 272 bits (696), Expect = 8e-73
Identities = 130/238 (54%), Positives = 172/238 (72%)
Frame = -1
Query: 835 LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
LFYL+ + KEELL MW +L SE V++VF +++ PN NG V LPW+ + L E
Sbjct: 405 LFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWN-DEPLAAE 463
Query: 655 TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
T +++ EL R+N QGILTINSQP ING S+D I GWG GY++QKAYLEFF E
Sbjct: 464 TSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAE 523
Query: 475 SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
+L++VL +Y RVNYH+V K +ITN ++ A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 524 ALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFW 583
Query: 295 KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDN++ +NCL+++V+ L+
Sbjct: 584 KDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLE 641
>sp|Q9WU20|MTHR_MOUSE Methylenetetrahydrofolate reductase
Length = 654
Score = 269 bits (687), Expect = 9e-72
Identities = 126/238 (52%), Positives = 171/238 (71%)
Frame = -1
Query: 835 LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
LFYL+ + +EELL MW +L SE V++VF H+++ PN +G+ V LPW+ + L E
Sbjct: 404 LFYLKSKSpreELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWN-DEPLAAE 462
Query: 655 TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
T +++ EL R+N GILTINSQP IN S+D + GWG GY++QKAYLEFF +E
Sbjct: 463 TSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVE 522
Query: 475 SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
+L++VL Y RVNYHIV K +ITN ++ A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 523 ALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFW 582
Query: 295 KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDNE+ ++CL+++V+ +
Sbjct: 583 KDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFE 640
>sp|Q60HE5|MTHR_MACFA Methylenetetrahydrofolate reductase
Length = 656
Score = 268 bits (685), Expect = 1e-71
Identities = 127/238 (53%), Positives = 171/238 (71%)
Frame = -1
Query: 835 LFYLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENE 656
LFYL+ + +E LL MW +L SE V++VF +++ PN NG V LPW+ + L E
Sbjct: 405 LFYLKSKSpreLLLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWN-DEPLAAE 463
Query: 655 TKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIE 476
T +++ EL R+N QGILTINSQP ING S+D + GWG GY++QKAYLEFF E
Sbjct: 464 TSLLKEELLRVNRQGILTINSQPNINGKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETAE 523
Query: 475 SLIKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYW 296
+L++VL +Y RVNYH+V K +ITN ++ A+TWG+FPG EIIQPTVVDP++F +W
Sbjct: 524 ALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFW 583
Query: 295 KREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
K EAF LWI+ WG LYEE S SR +IQY+HDNY+LVNLVDN++ +NCL+++V+ L+
Sbjct: 584 KDEAFALWIEQWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDALE 641
>sp|Q17693|MTHR_CAEEL Probable methylenetetrahydrofolate reductase
Length = 663
Score = 218 bits (556), Expect = 1e-56
Identities = 112/232 (48%), Positives = 153/232 (65%), Gaps = 4/232 (1%)
Frame = -1
Query: 805 EELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPW-HPNTELENETKIIQAELG 629
++ L M+ + S DV +VF ++ITQ PNA+G V LPW T ++ ET +I +L
Sbjct: 429 DDRLAMFGANIESFEDVKRVFINYITQAPNADGVKVTVLPWTEAETGVQPETSLISEQLV 488
Query: 628 RLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESLIKVLN-E 452
NE GILT+NSQP +NG STD + GWG P GY YQKAYLE F+ + LI+++ E
Sbjct: 489 WCNENGILTVNSQPSVNGAPSTDPLVGWGKPGGYCYQKAYLECFMTAELSDKLIQIIERE 548
Query: 451 YSPRVNYHIVQSKDG--DITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKREAFD 278
+ RVNYH + +KD D TN ++ IA+TWGVFPGSEI QPTVVDPL+F+ W+ EA+
Sbjct: 549 FPVRVNYHAI-NKDSTFDKTNSEETTPIAVTWGVFPGSEIAQPTVVDPLSFRAWRDEAYQ 607
Query: 277 LWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIKENCLFKLVQKVLD 122
+W+ WG Y + S S VI+ +HD + LV LVDN++ K + LF ++QK LD
Sbjct: 608 MWMAQWGDFYPKESKSYGVIKAVHDEFRLVTLVDNDFQKPSVLFDVLQKALD 659
>sp|Q9SE60|MTHR1_ARATH Methylenetetrahydrofolate reductase 1 (AtMTHFR1)
Length = 592
Score = 184 bits (467), Expect = 3e-46
Identities = 91/222 (40%), Positives = 132/222 (59%)
Frame = -1
Query: 829 YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
+ R +G ++L W L S DV + F +KS PW L+ ETK
Sbjct: 370 FARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGN-------LKSSPWSELDGLQPETK 422
Query: 649 IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
II +LG++N G LTINSQP +N +S GWG P GY+YQKAYLEFF +++L
Sbjct: 423 IINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDKLDTL 482
Query: 469 IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
++ + P + Y V + ++N + + A+TWGVFP E+IQPT+VDP +FK WK
Sbjct: 483 VEKSKAF-PSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKD 541
Query: 289 EAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
EAF++W + W LY E+ SR++++ + ++YYLV+LVDN YI
Sbjct: 542 EAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYI 583
>sp|Q9SE94|MTHR1_MAIZE Methylenetetrahydrofolate reductase 1 (ZmMTHFR1)
Length = 593
Score = 181 bits (459), Expect = 2e-45
Identities = 96/224 (42%), Positives = 137/224 (61%), Gaps = 1/224 (0%)
Frame = -1
Query: 829 YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
+ R RG ++L + W L S D+ + F T G + S PW L+ ETK
Sbjct: 370 FTRPRGRGKKLQEEWAVPLKSVEDISERF------TNFCQGKLTSS-PWSELDGLQPETK 422
Query: 649 IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
II +L +N++G LTINSQP +NG +S GWG P GY+YQKAYLEFF ++ L
Sbjct: 423 IIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEKLDQL 482
Query: 469 IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAI-AITWGVFPGSEIIQPTVVDPLAFKYWK 293
I+ + + P + Y I +KDG+ + NA+ A+TWGVFPG EIIQPTVVD +F WK
Sbjct: 483 IEKIKAF-PSLTY-IAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWK 540
Query: 292 REAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYIK 161
EAF++W + WG ++ E SR++++ + YYLV+LVDN+Y++
Sbjct: 541 DEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQ 584
>sp|Q75HE6|MTHR_ORYSA Probable methylenetetrahydrofolate reductase
Length = 594
Score = 176 bits (446), Expect = 8e-44
Identities = 94/223 (42%), Positives = 134/223 (60%), Gaps = 1/223 (0%)
Frame = -1
Query: 829 YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
+ R RG ++L + W + S D+ + F + G + S PW L+ ETK
Sbjct: 370 FTRPRGRGKKLQEEWAVPVKSVEDINERFMNF------CQGKLTSS-PWSELDGLQPETK 422
Query: 649 IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
II +L ++N++G LTINSQP +NG S GWG P GY+YQKAYLEFF ++ L
Sbjct: 423 IIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEKLDQL 482
Query: 469 IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAI-AITWGVFPGSEIIQPTVVDPLAFKYWK 293
I+ + P + Y I +KDG+ + NA+ A+TWGVFPG EI+QPTVVD +F WK
Sbjct: 483 IEKSKAF-PSLTY-IAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWK 540
Query: 292 REAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
EAF++W K W L+ E SR+++ + +Y+LV+LVDN+YI
Sbjct: 541 DEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYI 583
>sp|O80585|MTHR2_ARATH Methylenetetrahydrofolate reductase 2 (AtMTHFR2)
Length = 594
Score = 166 bits (421), Expect = 6e-41
Identities = 86/222 (38%), Positives = 125/222 (56%)
Frame = -1
Query: 829 YLRVRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETK 650
+ R R ++L W L S D+ + F +KS PW L+ ET+
Sbjct: 370 FSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGN-------LKSSPWSELDGLQPETR 422
Query: 649 IIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESL 470
II +L ++N +G LTINSQP +N S GWG P GY+YQKAYLEFF ++++
Sbjct: 423 IINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEKLDAV 482
Query: 469 IKVLNEYSPRVNYHIVQSKDGDITNCDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
++ P + Y V + ++N + A+TWGVFP EIIQPT+VDP +F WK
Sbjct: 483 VEKCKAL-PSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKD 541
Query: 289 EAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNEYI 164
EAF+ W + W LY E SR +++ + ++YYLV+LV+N+YI
Sbjct: 542 EAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYI 583
>sp|Q10258|MTHR1_SCHPO Methylenetetrahydrofolate reductase 1
Length = 603
Score = 146 bits (368), Expect = 8e-35
Identities = 78/194 (40%), Positives = 119/194 (61%), Gaps = 3/194 (1%)
Frame = -1
Query: 700 VKSLPWHPNTELENETKIIQAELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIY 521
+ SLPW + + +E +I+ +L +N LTINSQP +NG +S+ +FGWG PNGY++
Sbjct: 400 ISSLPWS-DLPISDEADLIRDQLLSMNRNAFLTINSQPALNGEKSSHPVFGWGPPNGYVF 458
Query: 520 QKAYLEFFIHHRHIESLIKVLNEYSPRVN--YHIVQSKDGDI-TNCDDVNAIAITWGVFP 350
QK Y+EFF+H SL+ L E ++N + V +K+GD+ TN A+TWGVFP
Sbjct: 459 QKPYVEFFVH----PSLLNELKETVKKLNSVSYFVTNKNGDLDTNSQYEIPNAVTWGVFP 514
Query: 349 GSEIIQPTVVDPLAFKYWKREAFDLWIKHWGILYEENSISRQVIQYMHDNYYLVNLVDNE 170
EIIQPT+V+ +F WK EA+ L ++ W Y +SISR+++ M ++L +VDN+
Sbjct: 515 NREIIQPTIVESTSFLAWKDEAYSLGME-WANAYSPDSISRKLLVSMMKEWFLCVIVDND 573
Query: 169 YIKENCLFKLVQKV 128
+ LF + K+
Sbjct: 574 FQNGQSLFDVFNKM 587
>sp|P53128|MTHR2_YEAST Methylenetetrahydrofolate reductase 2
Length = 599
Score = 140 bits (354), Expect = 3e-33
Identities = 85/224 (37%), Positives = 130/224 (58%), Gaps = 5/224 (2%)
Frame = -1
Query: 820 VRGSKEELLDMWERQLGSENDVWKVFYHHITQTPNANGFMVKSLPWHPNTELENETKIIQ 641
+R S + L++W S NDV + +++ N N +K LPW + + +E I+
Sbjct: 376 IRQSANKCLELWSTPT-SINDVAFLVINYL----NGN---LKCLPWS-DIPINDEINPIK 426
Query: 640 AELGRLNEQGILTINSQPCINGVESTDDIFGWGLPNGYIYQKAYLEFFIHHRHIESLIKV 461
A L LN+ I+TINSQP +NG+ S D I GWG +GY+YQK YLEF + + LI
Sbjct: 427 AHLIELNQHSIITINSQPQVNGIRSNDKIHGWGPKDGYVYQKQYLEFMLPKTKLPKLIDT 486
Query: 460 L--NEYSPRVNYHIVQSKDGDITN-CDDVNAIAITWGVFPGSEIIQPTVVDPLAFKYWKR 290
L NE+ + Y + S+ ++N D+ + A+TWG+FPG EI+QPT+V+ ++F WK
Sbjct: 487 LKNNEF---LTYFAIDSQGDLLSNHPDNSKSNAVTWGIFPGREILQPTIVEKISFLAWKE 543
Query: 289 EAFDLWIKHWGILYE--ENSISRQVIQYMHDNYYLVNLVDNEYI 164
E + + + W + + S Q IQ + D+Y LVN+VDN+YI
Sbjct: 544 EFYHI-LNEWKLNMNKYDKPHSAQFIQSLIDDYCLVNIVDNDYI 586
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,152,736
Number of Sequences: 369166
Number of extensions: 1956334
Number of successful extensions: 5125
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5110
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)