Planaria EST Database


DrC_02296

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02296
         (832 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P35033|TRY3_SALSA  Trypsin III precursor                        95   3e-19
sp|Q8K1T0|TMPS3_MOUSE  Transmembrane protease, serine 3            94   4e-19
sp|P35030|TRY3_HUMAN  Trypsin III precursor (Brain trypsinog...    94   4e-19
sp|P26262|KLKB1_MOUSE  Plasma kallikrein precursor (Plasma p...    92   2e-18
sp|P50343|MCPT6_RAT  Mast cell protease 6 precursor (rMCP-6)...    92   2e-18
sp|P00760|TRY1_BOVIN  Cationic trypsin precursor (Beta-tryps...    92   2e-18
sp|P00761|TRYP_PIG  Trypsin precursor                              91   3e-18
sp|P32822|TRYB_RAT  Trypsin V-B precursor                          91   4e-18
sp|P14272|KLKB1_RAT  Plasma kallikrein precursor (Plasma pre...    91   5e-18
sp|P32821|TRYA_RAT  Trypsin V-A precursor                          90   9e-18
>sp|P35033|TRY3_SALSA Trypsin III precursor
          Length = 238

 Score = 94.7 bits (234), Expect = 3e-19
 Identities = 59/177 (33%), Positives = 98/177 (55%), Gaps = 1/177 (0%)
 Frame = +1

Query: 259 KIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPSV 438
           K+I+HP      Y++R+    +D+DI L+KL +P  ++  V  ST++LP S  ++     
Sbjct: 84  KVIMHP-----SYNSRN----LDNDIMLIKLSKPASLNSYV--STVALPSSCASS----- 127

Query: 439 GSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
           G+ C+V GWG +    S  P   +   L I  +S C  + P ++  N    FCAG+++  
Sbjct: 128 GTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAYPGQITSNM---FCAGFMEGG 184

Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
              C GDSG  ++C+ Q    + GVVS G+G    + PGV+T+V ++R+WI   ++S
Sbjct: 185 KDSCQGDSGGPVVCNGQ----LQGVVSWGYGCAQRNKPGVYTKVCNYRSWISSTMSS 237
>sp|Q8K1T0|TMPS3_MOUSE Transmembrane protease, serine 3
          Length = 453

 Score = 94.4 bits (233), Expect = 4e-19
 Identities = 77/251 (30%), Positives = 107/251 (42%)
 Frame = +1

Query: 22  WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
           W++TAAHC        VY+L   + W  +VGL+ +            +   V S      
Sbjct: 251 WIVTAAHC--------VYDLYHPKSWTVQVGLVSL------------MDSPVPSHL---- 286

Query: 202 RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
                            + KII H +Y P          R+ +DIALMKL EPL   +  
Sbjct: 287 -----------------VEKIIYHSKYKPK---------RLGNDIALMKLSEPLTFDET- 319

Query: 382 GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
            I  I LP+S  N  +P  G  C   GWG  +     +PV   A + +  N  C      
Sbjct: 320 -IQPICLPNSEEN--FPD-GKLCWTSGWGATEDGGDASPVLNHAAVPLISNKICNHRDVY 375

Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
              ++P S  CAGYLK     C GDSG  L+C  +    + G  S G G    + PGV+T
Sbjct: 376 GGIISP-SMLCAGYLKGGVDSCQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYT 434

Query: 742 RVSSFRNWIDD 774
           R++SF +WI +
Sbjct: 435 RITSFLDWIHE 445
>sp|P35030|TRY3_HUMAN Trypsin III precursor (Brain trypsinogen) (Mesotrypsinogen)
           (Trypsin IV)
          Length = 304

 Score = 94.4 bits (233), Expect = 4e-19
 Identities = 68/195 (34%), Positives = 103/195 (52%), Gaps = 4/195 (2%)
 Frame = +1

Query: 214 GKNKFKVTTDD---LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVG 384
           G++  KV   +   ++ +KII HP+Y       RD+L   D+DI L+KL  P  ++  V 
Sbjct: 131 GEHNIKVLEGNEQFINAAKIIRHPKY------NRDTL---DNDIMLIKLSSPAVINARV- 180

Query: 385 ISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPT 561
            STISLP     T  P+ G+EC++ GWG      +  P   K     +   +EC+ S P 
Sbjct: 181 -STISLP-----TAPPAAGTECLISGWGNTLSFGADYPDELKCLDAPVLTQAECKASYPG 234

Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
           K+     S FC G+L+     C  DSG  ++C+ Q    + GVVS GHG    + PGV+T
Sbjct: 235 KITN---SMFCVGFLEGGKDSCQRDSGGPVVCNGQ----LQGVVSWGHGCAWKNRPGVYT 287

Query: 742 RVSSFRNWIDDVVAS 786
           +V ++ +WI D +A+
Sbjct: 288 KVYNYVDWIKDTIAA 302
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
            (Fletcher factor) [Contains: Plasma kallikrein heavy
            chain; Plasma kallikrein light chain]
          Length = 638

 Score = 92.0 bits (227), Expect = 2e-18
 Identities = 69/249 (27%), Positives = 106/249 (42%)
 Frame = +1

Query: 22   WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
            W+LTAAHCF   DGI   ++     W    G++ +  + K+                   
Sbjct: 428  WVLTAAHCF---DGIPYPDV-----WRIYGGILSLSEITKE------------------- 460

Query: 202  RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
                        T    I ++IIH  Y          +   + DIAL+KL+ PL  ++  
Sbjct: 461  ------------TPSSRIKELIIHQEY---------KVSEGNYDIALIKLQTPLNYTEFQ 499

Query: 382  GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
                I LP  +   T   + + C V GWG  K +     + + A + +  N EC+K    
Sbjct: 500  --KPICLPSKADTNT---IYTNCWVTGWGYTKEQGETQNILQKATIPLVPNEECQKKY-- 552

Query: 562  KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
            + Y+      CAGY +     C GDSG  L+C + G+  + G+ S G G    D PGV+T
Sbjct: 553  RDYVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQLVGITSWGEGCGRKDQPGVYT 612

Query: 742  RVSSFRNWI 768
            +VS + +WI
Sbjct: 613  KVSEYMDWI 621
>sp|P50343|MCPT6_RAT Mast cell protease 6 precursor (rMCP-6) (Tryptase)
          Length = 274

 Score = 91.7 bits (226), Expect = 2e-18
 Identities = 69/185 (37%), Positives = 97/185 (52%), Gaps = 11/185 (5%)
 Frame = +1

Query: 247 LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTT 426
           L +++ ++HP Y    Y+  D      +DIAL++L+ P+ VS    I  ISLP +S   T
Sbjct: 101 LTVNRTVVHPHY----YTVEDG-----ADIALLELEIPVNVS--THIHPISLPPAS--ET 147

Query: 427 WPSVGSECIVVGWGCIKVRDSPNPVA-----KLAKLQIWENSECEKSIPTKLYMN---PL 582
           +PS G+ C V GWG I   DS  P+      K  K+ I ENS C++   T LY     P+
Sbjct: 148 FPS-GTSCWVTGWGDI---DSDEPLLPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDVPI 203

Query: 583 SD---FCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSS 753
                 CAG  +     C GDSG  L+C  +G    AGVVS G G    + PG++TRV+ 
Sbjct: 204 VQDGMLCAGNTRSDS--CQGDSGGPLVCKVKGTWLQAGVVSWGEGCAEANRPGIYTRVTY 261

Query: 754 FRNWI 768
           + +WI
Sbjct: 262 YLDWI 266
>sp|P00760|TRY1_BOVIN Cationic trypsin precursor (Beta-trypsin) [Contains: Alpha-trypsin
           chain 1; Alpha-trypsin chain 2]
          Length = 243

 Score = 91.7 bits (226), Expect = 2e-18
 Identities = 62/178 (34%), Positives = 94/178 (52%), Gaps = 1/178 (0%)
 Frame = +1

Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
           SK I+HP Y             +++DI L+KLK    ++  V  ++ISLP     T+  S
Sbjct: 88  SKSIVHPSYNSN---------TLNNDIMLIKLKSAASLNSRV--ASISLP-----TSCAS 131

Query: 436 VGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKK 612
            G++C++ GWG  K   +  P V K  K  I  +S C+ + P ++  N    FCAGYL+ 
Sbjct: 132 AGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNM---FCAGYLEG 188

Query: 613 QYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
               C GDSG  ++C   GK  + G+VS G G    + PGV+T+V ++ +WI   +AS
Sbjct: 189 GKDSCQGDSGGPVVCS--GK--LQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIAS 242
>sp|P00761|TRYP_PIG Trypsin precursor
          Length = 231

 Score = 91.3 bits (225), Expect = 3e-18
 Identities = 57/181 (31%), Positives = 95/181 (52%), Gaps = 1/181 (0%)
 Frame = +1

Query: 247 LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTT 426
           ++ +KII HP +             +D+DI L+KL  P  ++  V  +T+SLP S     
Sbjct: 73  INAAKIITHPNFNGN---------TLDNDIMLIKLSSPATLNSRV--ATVSLPRSCA--- 118

Query: 427 WPSVGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGY 603
             + G+EC++ GWG  K   S  P + +  K  +  +S C+ S P ++  N +   C G+
Sbjct: 119 --AAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMI---CVGF 173

Query: 604 LKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVA 783
           L+     C GDSG  ++C+ Q    + G+VS G+G    + PGV+T+V ++ NWI   +A
Sbjct: 174 LEGGKDSCQGDSGGPVVCNGQ----LQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIA 229

Query: 784 S 786
           +
Sbjct: 230 A 230
>sp|P32822|TRYB_RAT Trypsin V-B precursor
          Length = 246

 Score = 90.9 bits (224), Expect = 4e-18
 Identities = 58/177 (32%), Positives = 90/177 (50%)
 Frame = +1

Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
           +K+I+HP Y           + +D+DI L+KLK P  ++  V  STI LP        P+
Sbjct: 92  AKMILHPDY---------DKWTVDNDIMLIKLKSPATLNSKV--STIPLPQYC-----PT 135

Query: 436 VGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
            G+EC+V GWG +K       V +     +  +S C K+ P ++  N    FC G+L+  
Sbjct: 136 AGTECLVSGWGVLKFGFESPSVLQCLDAPVLSDSVCHKAYPRQITNNM---FCLGFLEGG 192

Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
              C  DSG  ++C+ +    + G+VS G G      PGV+T+V ++ NWI   VA+
Sbjct: 193 KDSCQYDSGGPVVCNGE----VQGIVSWGDGCALEGKPGVYTKVCNYLNWIQQTVAA 245
>sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
            (Fletcher factor) [Contains: Plasma kallikrein heavy
            chain; Plasma kallikrein light chain]
          Length = 638

 Score = 90.5 bits (223), Expect = 5e-18
 Identities = 69/255 (27%), Positives = 108/255 (42%)
 Frame = +1

Query: 22   WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
            W+LTAAHCF   DGI   ++     W    G++ +  +  K                   
Sbjct: 428  WILTAAHCF---DGIPYPDV-----WRIYGGILNLSEITNK------------------- 460

Query: 202  RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
                        T    I ++IIH +Y          +     DIAL+KL+ PL  ++  
Sbjct: 461  ------------TPFSSIKELIIHQKY---------KMSEGSYDIALIKLQTPLNYTEFQ 499

Query: 382  GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
                I LP  +   T   + + C V GWG  K R     + + A + +  N EC+K    
Sbjct: 500  --KPICLPSKADTNT---IYTNCWVTGWGYTKERGETQNILQKATIPLVPNEECQKKY-- 552

Query: 562  KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
            + Y+      CAGY +     C GDSG  L+C + G+  + G+ S G G    + PGV+T
Sbjct: 553  RDYVITKQMICAGYKEGGIDACKGDSGGPLVCKHSGRWQLVGITSWGEGCARKEQPGVYT 612

Query: 742  RVSSFRNWIDDVVAS 786
            +V+ + +WI + + S
Sbjct: 613  KVAEYIDWILEKIQS 627
>sp|P32821|TRYA_RAT Trypsin V-A precursor
          Length = 246

 Score = 89.7 bits (221), Expect = 9e-18
 Identities = 57/176 (32%), Positives = 89/176 (50%)
 Frame = +1

Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
           +K+I+HP Y           + +D+DI L+KLK P  ++  V  STI LP        P+
Sbjct: 92  AKMILHPDY---------DKWTVDNDIMLIKLKSPATLNSKV--STIPLPQYC-----PT 135

Query: 436 VGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
            G+EC+V GWG +K       V +     +  +S C K+ P ++  N    FC G+L+  
Sbjct: 136 AGTECLVSGWGVLKFGFESPSVLQCLDAPVLSDSVCHKAYPRQITNNM---FCLGFLEGG 192

Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVA 783
              C  DSG  ++C+ +    + G+VS G G      PGV+T+V ++ NWI   +A
Sbjct: 193 KDSCQYDSGGPVVCNGE----VQGIVSWGDGCALEGKPGVYTKVCNYLNWIHQTIA 244
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,200,247
Number of Sequences: 369166
Number of extensions: 2191012
Number of successful extensions: 6791
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5678
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6244
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)