Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_C03
(832 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35033|TRY3_SALSA Trypsin III precursor 95 3e-19
sp|Q8K1T0|TMPS3_MOUSE Transmembrane protease, serine 3 94 4e-19
sp|P35030|TRY3_HUMAN Trypsin III precursor (Brain trypsinog... 94 4e-19
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 92 2e-18
sp|P50343|MCPT6_RAT Mast cell protease 6 precursor (rMCP-6)... 92 2e-18
sp|P00760|TRY1_BOVIN Cationic trypsin precursor (Beta-tryps... 92 2e-18
sp|P00761|TRYP_PIG Trypsin precursor 91 3e-18
sp|P32822|TRYB_RAT Trypsin V-B precursor 91 4e-18
sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma pre... 91 5e-18
sp|P32821|TRYA_RAT Trypsin V-A precursor 90 9e-18
>sp|P35033|TRY3_SALSA Trypsin III precursor
Length = 238
Score = 94.7 bits (234), Expect = 3e-19
Identities = 59/177 (33%), Positives = 98/177 (55%), Gaps = 1/177 (0%)
Frame = +1
Query: 259 KIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPSV 438
K+I+HP Y++R+ +D+DI L+KL +P ++ V ST++LP S ++
Sbjct: 84 KVIMHP-----SYNSRN----LDNDIMLIKLSKPASLNSYV--STVALPSSCASS----- 127
Query: 439 GSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
G+ C+V GWG + S P + L I +S C + P ++ N FCAG+++
Sbjct: 128 GTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAYPGQITSNM---FCAGFMEGG 184
Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
C GDSG ++C+ Q + GVVS G+G + PGV+T+V ++R+WI ++S
Sbjct: 185 KDSCQGDSGGPVVCNGQ----LQGVVSWGYGCAQRNKPGVYTKVCNYRSWISSTMSS 237
>sp|Q8K1T0|TMPS3_MOUSE Transmembrane protease, serine 3
Length = 453
Score = 94.4 bits (233), Expect = 4e-19
Identities = 77/251 (30%), Positives = 107/251 (42%)
Frame = +1
Query: 22 WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
W++TAAHC VY+L + W +VGL+ + + V S
Sbjct: 251 WIVTAAHC--------VYDLYHPKSWTVQVGLVSL------------MDSPVPSHL---- 286
Query: 202 RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
+ KII H +Y P R+ +DIALMKL EPL +
Sbjct: 287 -----------------VEKIIYHSKYKPK---------RLGNDIALMKLSEPLTFDET- 319
Query: 382 GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
I I LP+S N +P G C GWG + +PV A + + N C
Sbjct: 320 -IQPICLPNSEEN--FPD-GKLCWTSGWGATEDGGDASPVLNHAAVPLISNKICNHRDVY 375
Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
++P S CAGYLK C GDSG L+C + + G S G G + PGV+T
Sbjct: 376 GGIISP-SMLCAGYLKGGVDSCQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYT 434
Query: 742 RVSSFRNWIDD 774
R++SF +WI +
Sbjct: 435 RITSFLDWIHE 445
>sp|P35030|TRY3_HUMAN Trypsin III precursor (Brain trypsinogen) (Mesotrypsinogen)
(Trypsin IV)
Length = 304
Score = 94.4 bits (233), Expect = 4e-19
Identities = 68/195 (34%), Positives = 103/195 (52%), Gaps = 4/195 (2%)
Frame = +1
Query: 214 GKNKFKVTTDD---LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVG 384
G++ KV + ++ +KII HP+Y RD+L D+DI L+KL P ++ V
Sbjct: 131 GEHNIKVLEGNEQFINAAKIIRHPKY------NRDTL---DNDIMLIKLSSPAVINARV- 180
Query: 385 ISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPT 561
STISLP T P+ G+EC++ GWG + P K + +EC+ S P
Sbjct: 181 -STISLP-----TAPPAAGTECLISGWGNTLSFGADYPDELKCLDAPVLTQAECKASYPG 234
Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
K+ S FC G+L+ C DSG ++C+ Q + GVVS GHG + PGV+T
Sbjct: 235 KITN---SMFCVGFLEGGKDSCQRDSGGPVVCNGQ----LQGVVSWGHGCAWKNRPGVYT 287
Query: 742 RVSSFRNWIDDVVAS 786
+V ++ +WI D +A+
Sbjct: 288 KVYNYVDWIKDTIAA 302
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 92.0 bits (227), Expect = 2e-18
Identities = 69/249 (27%), Positives = 106/249 (42%)
Frame = +1
Query: 22 WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
W+LTAAHCF DGI ++ W G++ + + K+
Sbjct: 428 WVLTAAHCF---DGIPYPDV-----WRIYGGILSLSEITKE------------------- 460
Query: 202 RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
T I ++IIH Y + + DIAL+KL+ PL ++
Sbjct: 461 ------------TPSSRIKELIIHQEY---------KVSEGNYDIALIKLQTPLNYTEFQ 499
Query: 382 GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
I LP + T + + C V GWG K + + + A + + N EC+K
Sbjct: 500 --KPICLPSKADTNT---IYTNCWVTGWGYTKEQGETQNILQKATIPLVPNEECQKKY-- 552
Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
+ Y+ CAGY + C GDSG L+C + G+ + G+ S G G D PGV+T
Sbjct: 553 RDYVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQLVGITSWGEGCGRKDQPGVYT 612
Query: 742 RVSSFRNWI 768
+VS + +WI
Sbjct: 613 KVSEYMDWI 621
>sp|P50343|MCPT6_RAT Mast cell protease 6 precursor (rMCP-6) (Tryptase)
Length = 274
Score = 91.7 bits (226), Expect = 2e-18
Identities = 69/185 (37%), Positives = 97/185 (52%), Gaps = 11/185 (5%)
Frame = +1
Query: 247 LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTT 426
L +++ ++HP Y Y+ D +DIAL++L+ P+ VS I ISLP +S T
Sbjct: 101 LTVNRTVVHPHY----YTVEDG-----ADIALLELEIPVNVS--THIHPISLPPAS--ET 147
Query: 427 WPSVGSECIVVGWGCIKVRDSPNPVA-----KLAKLQIWENSECEKSIPTKLYMN---PL 582
+PS G+ C V GWG I DS P+ K K+ I ENS C++ T LY P+
Sbjct: 148 FPS-GTSCWVTGWGDI---DSDEPLLPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDVPI 203
Query: 583 SD---FCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSS 753
CAG + C GDSG L+C +G AGVVS G G + PG++TRV+
Sbjct: 204 VQDGMLCAGNTRSDS--CQGDSGGPLVCKVKGTWLQAGVVSWGEGCAEANRPGIYTRVTY 261
Query: 754 FRNWI 768
+ +WI
Sbjct: 262 YLDWI 266
>sp|P00760|TRY1_BOVIN Cationic trypsin precursor (Beta-trypsin) [Contains: Alpha-trypsin
chain 1; Alpha-trypsin chain 2]
Length = 243
Score = 91.7 bits (226), Expect = 2e-18
Identities = 62/178 (34%), Positives = 94/178 (52%), Gaps = 1/178 (0%)
Frame = +1
Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
SK I+HP Y +++DI L+KLK ++ V ++ISLP T+ S
Sbjct: 88 SKSIVHPSYNSN---------TLNNDIMLIKLKSAASLNSRV--ASISLP-----TSCAS 131
Query: 436 VGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKK 612
G++C++ GWG K + P V K K I +S C+ + P ++ N FCAGYL+
Sbjct: 132 AGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNM---FCAGYLEG 188
Query: 613 QYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
C GDSG ++C GK + G+VS G G + PGV+T+V ++ +WI +AS
Sbjct: 189 GKDSCQGDSGGPVVCS--GK--LQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIAS 242
>sp|P00761|TRYP_PIG Trypsin precursor
Length = 231
Score = 91.3 bits (225), Expect = 3e-18
Identities = 57/181 (31%), Positives = 95/181 (52%), Gaps = 1/181 (0%)
Frame = +1
Query: 247 LHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTT 426
++ +KII HP + +D+DI L+KL P ++ V +T+SLP S
Sbjct: 73 INAAKIITHPNFNGN---------TLDNDIMLIKLSSPATLNSRV--ATVSLPRSCA--- 118
Query: 427 WPSVGSECIVVGWGCIKVRDSPNP-VAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGY 603
+ G+EC++ GWG K S P + + K + +S C+ S P ++ N + C G+
Sbjct: 119 --AAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMI---CVGF 173
Query: 604 LKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVA 783
L+ C GDSG ++C+ Q + G+VS G+G + PGV+T+V ++ NWI +A
Sbjct: 174 LEGGKDSCQGDSGGPVVCNGQ----LQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIA 229
Query: 784 S 786
+
Sbjct: 230 A 230
>sp|P32822|TRYB_RAT Trypsin V-B precursor
Length = 246
Score = 90.9 bits (224), Expect = 4e-18
Identities = 58/177 (32%), Positives = 90/177 (50%)
Frame = +1
Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
+K+I+HP Y + +D+DI L+KLK P ++ V STI LP P+
Sbjct: 92 AKMILHPDY---------DKWTVDNDIMLIKLKSPATLNSKV--STIPLPQYC-----PT 135
Query: 436 VGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
G+EC+V GWG +K V + + +S C K+ P ++ N FC G+L+
Sbjct: 136 AGTECLVSGWGVLKFGFESPSVLQCLDAPVLSDSVCHKAYPRQITNNM---FCLGFLEGG 192
Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVAS 786
C DSG ++C+ + + G+VS G G PGV+T+V ++ NWI VA+
Sbjct: 193 KDSCQYDSGGPVVCNGE----VQGIVSWGDGCALEGKPGVYTKVCNYLNWIQQTVAA 245
>sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 90.5 bits (223), Expect = 5e-18
Identities = 69/255 (27%), Positives = 108/255 (42%)
Frame = +1
Query: 22 WLLTAAHCFFPSDGINVYNLTKGEYWHARVGLMKVDFLYKKHGIKDKVKEKVKSFFNKAY 201
W+LTAAHCF DGI ++ W G++ + + K
Sbjct: 428 WILTAAHCF---DGIPYPDV-----WRIYGGILNLSEITNK------------------- 460
Query: 202 RKLFGKNKFKVTTDDLHISKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLV 381
T I ++IIH +Y + DIAL+KL+ PL ++
Sbjct: 461 ------------TPFSSIKELIIHQKY---------KMSEGSYDIALIKLQTPLNYTEFQ 499
Query: 382 GISTISLPDSSVNTTWPSVGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPT 561
I LP + T + + C V GWG K R + + A + + N EC+K
Sbjct: 500 --KPICLPSKADTNT---IYTNCWVTGWGYTKERGETQNILQKATIPLVPNEECQKKY-- 552
Query: 562 KLYMNPLSDFCAGYLKKQYGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFT 741
+ Y+ CAGY + C GDSG L+C + G+ + G+ S G G + PGV+T
Sbjct: 553 RDYVITKQMICAGYKEGGIDACKGDSGGPLVCKHSGRWQLVGITSWGEGCARKEQPGVYT 612
Query: 742 RVSSFRNWIDDVVAS 786
+V+ + +WI + + S
Sbjct: 613 KVAEYIDWILEKIQS 627
>sp|P32821|TRYA_RAT Trypsin V-A precursor
Length = 246
Score = 89.7 bits (221), Expect = 9e-18
Identities = 57/176 (32%), Positives = 89/176 (50%)
Frame = +1
Query: 256 SKIIIHPRYIPTGYSTRDSLFRMDSDIALMKLKEPLPVSQLVGISTISLPDSSVNTTWPS 435
+K+I+HP Y + +D+DI L+KLK P ++ V STI LP P+
Sbjct: 92 AKMILHPDY---------DKWTVDNDIMLIKLKSPATLNSKV--STIPLPQYC-----PT 135
Query: 436 VGSECIVVGWGCIKVRDSPNPVAKLAKLQIWENSECEKSIPTKLYMNPLSDFCAGYLKKQ 615
G+EC+V GWG +K V + + +S C K+ P ++ N FC G+L+
Sbjct: 136 AGTECLVSGWGVLKFGFESPSVLQCLDAPVLSDSVCHKAYPRQITNNM---FCLGFLEGG 192
Query: 616 YGLCHGDSGSGLICDYQGKQTIAGVVSRGHGGDPGDYPGVFTRVSSFRNWIDDVVA 783
C DSG ++C+ + + G+VS G G PGV+T+V ++ NWI +A
Sbjct: 193 KDSCQYDSGGPVVCNGE----VQGIVSWGDGCALEGKPGVYTKVCNYLNWIHQTIA 244
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,200,247
Number of Sequences: 369166
Number of extensions: 2191012
Number of successful extensions: 6791
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5678
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6244
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)