Planaria EST Database


DrC_02283

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02283
         (765 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q61084|M3K3_MOUSE  Mitogen-activated protein kinase kinas...   116   8e-26
sp|Q99759|M3K3_HUMAN  Mitogen-activated protein kinase kinas...   116   8e-26
sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...   114   4e-25
sp|Q12263|GIN4_YEAST  Serine/threonine-protein kinase GIN4        113   6e-25
sp|Q61083|M3K2_MOUSE  Mitogen-activated protein kinase kinas...   113   6e-25
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...   112   8e-25
sp|Q9SZ67|WRK19_ARATH  Probable WRKY transcription factor 19...   112   1e-24
sp|P32491|MKK2_YEAST  MAP kinase kinase MKK2/SSP33                112   1e-24
sp|Q9Y2U5|M3K2_HUMAN  Mitogen-activated protein kinase kinas...   110   4e-24
sp|P32490|MKK1_YEAST  MAP kinase kinase MKK1/SSP32                110   5e-24
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  116 bits (290), Expect = 8e-26
 Identities = 69/179 (38%), Positives = 101/179 (56%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+KD +     +  S   K++++ILEG+ ++HS  I+HRDIK  N+L D    +KL DF
Sbjct: 449 GSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDF 508

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK   L++ C S T   +  GTP +++PE+  GE Y   AD+WS G +V+ M+T K P
Sbjct: 509 GASK--RLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPP 566

Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKHSIFQ 679
           +      A +FKI T       P   ISE  + F+  +   E  +RPSA++LL H   Q
Sbjct: 567 WAEYEAMAAIFKIATQPTNPQLP-SHISEHGRDFLRRIFV-EARQRPSAEELLTHHFAQ 623
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  116 bits (290), Expect = 8e-26
 Identities = 69/179 (38%), Positives = 101/179 (56%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+KD +     +  S   K++++ILEG+ ++HS  I+HRDIK  N+L D    +KL DF
Sbjct: 449 GSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDF 508

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK   L++ C S T   +  GTP +++PE+  GE Y   AD+WS G +V+ M+T K P
Sbjct: 509 GASK--RLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPP 566

Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKHSIFQ 679
           +      A +FKI T       P   ISE  + F+  +   E  +RPSA++LL H   Q
Sbjct: 567 WAEYEAMAAIFKIATQPTNPQLP-SHISEHGRDFLRRIFV-EARQRPSAEELLTHHFAQ 623
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score =  114 bits (284), Expect = 4e-25
 Identities = 67/176 (38%), Positives = 101/176 (57%), Gaps = 1/176 (0%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+  L++K      S V  ++ ++L GLE++H+  I+HRDIK  N+L+D+   +KL DF
Sbjct: 156 GSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADF 215

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK +   +   + +   +  GTP ++APE+ L   + F ADIWS G +VI M+TGK P
Sbjct: 216 GASKQV---AELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAP 272

Query: 503 Y-HSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
           +       A +F IGT+K     P + IS     F+   L  E + RP+A +LLKH
Sbjct: 273 WSQQYKEIAAIFHIGTTKSHPPIP-DNISSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4
          Length = 1142

 Score =  113 bits (282), Expect = 6e-25
 Identities = 64/185 (34%), Positives = 105/185 (56%), Gaps = 4/185 (2%)
 Frame = +2

Query: 140 QGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLID 319
           +G L +L+ +   +   + I+F ++I+ G+ + H+ GI+HRD+KPEN+LLDH   +K+ D
Sbjct: 115 KGELFNLLVERGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHKYNIKIAD 174

Query: 320 FGLSKSLHLKSFCESTTKTV-TFCGTPNYLAPEIFLGEPYY-FPADIWSTGVSVIHMITG 493
           FG++         E+  K + T CG+P+Y APEI  G PY  F +D+WS GV +  ++TG
Sbjct: 175 FGMAA-------LETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTG 227

Query: 494 KTPYHSLP--IPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
           + P+      I  ++ K+    + E    + IS   Q  I  +LT +   R   +D+LKH
Sbjct: 228 RLPFDEEDGNIRTLLLKV-QKGEFEMPSDDEISREAQDLIRKILTVDPERRIKTRDILKH 286

Query: 668 SIFQK 682
            + QK
Sbjct: 287 PLLQK 291
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
           kinase 2) (MEK kinase 2) (MEKK 2)
          Length = 619

 Score =  113 bits (282), Expect = 6e-25
 Identities = 65/175 (37%), Positives = 99/175 (56%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+KD +     +  +   K++++ILEG+ ++HS  I+HRDIK  N+L D    +KL DF
Sbjct: 443 GSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDF 502

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK   L++ C S T   +  GTP +++PE+  GE Y   ADIWS   +V+ M+T K P
Sbjct: 503 GASK--RLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPP 560

Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
           +      A +FKI T       P   +S+  + F+  +   E   RPSA++LL+H
Sbjct: 561 WAEFEAMAAIFKIATQPTNPKLP-PHVSDYTRDFLKRIFV-EAKLRPSAEELLRH 613
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score =  112 bits (281), Expect = 8e-25
 Identities = 66/176 (37%), Positives = 102/176 (57%), Gaps = 1/176 (0%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+  L++K      S V  +++++L GLE++H+  I+HRDIK  N+L+D+   +KL DF
Sbjct: 157 GSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADF 216

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK +   +   + T   +  GTP ++APE+ L   + F ADIWS G +VI M+TGK P
Sbjct: 217 GASKQV---AELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAP 273

Query: 503 Y-HSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
           +       A +F IGT+K     P + +S   + F+   L    + RP+A +LLKH
Sbjct: 274 WSQQYKEVAAIFFIGTTKSHPPIP-DTLSSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
          Length = 1895

 Score =  112 bits (280), Expect = 1e-24
 Identities = 69/183 (37%), Positives = 106/183 (57%), Gaps = 6/183 (3%)
 Frame = +2

Query: 137  SQGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLI 316
            +QGSL+ L  +++ +  S V  ++++IL+GL+++H +G IHR+IK  N+L+D N T+KL 
Sbjct: 1708 TQGSLRKLYQRNQ-LGDSVVSLYTRQILDGLKYLHDKGFIHRNIKCANVLVDANGTVKLA 1766

Query: 317  DFGLSKSLHLKSFCESTTKTVTFCGTP--NYLAPEIFLG----EPYYFPADIWSTGVSVI 478
            DFGL+K + L               TP  N++APE+ L     + Y  PADIWS G +V+
Sbjct: 1767 DFGLAKVMSL-------------WRTPYWNWMAPEVILNPKDYDGYGTPADIWSLGCTVL 1813

Query: 479  HMITGKTPYHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDL 658
             M+TG+ PY  L I   ++ IGT K  +   +  +S   + FI   L     ERP+A +L
Sbjct: 1814 EMLTGQIPYSDLEIGTALYNIGTGKLPKIPDI--LSLDARDFILTCLKVNPEERPTAAEL 1871

Query: 659  LKH 667
            L H
Sbjct: 1872 LNH 1874
>sp|P32491|MKK2_YEAST MAP kinase kinase MKK2/SSP33
          Length = 506

 Score =  112 bits (279), Expect = 1e-24
 Identities = 62/185 (33%), Positives = 102/185 (55%), Gaps = 9/185 (4%)
 Frame = +2

Query: 140 QGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLID 319
           + + K+L+ +   I    + K ++ +L GL ++H + +IHRDIKP+N+LL+    +KL D
Sbjct: 301 EATYKNLLKRGGRISERVIGKIAESVLRGLSYLHERKVIHRDIKPQNILLNEKGEIKLCD 360

Query: 320 FGLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKT 499
           FG+S            +  +TF GT  Y+APE   G+PY    D+WS G++++ +  G+ 
Sbjct: 361 FGVSGE-------AVNSLAMTFTGTSFYMAPERIQGQPYSVTCDVWSLGLTLLEVAGGRF 413

Query: 500 PYHS-------LPIPAVMFKIGTSKKIEFTPLEPI--SETMQSFIDYLLTPEQSERPSAK 652
           P+ S        PI  +   +  S +++  P   I  S+T +SFIDY L  +  ERPS +
Sbjct: 414 PFESDKITQNVAPIELLTMILTFSPQLKDEPELDISWSKTFRSFIDYCLKKDARERPSPR 473

Query: 653 DLLKH 667
            +LKH
Sbjct: 474 QMLKH 478
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
           kinase 2) (MEK kinase 2) (MEKK 2)
          Length = 618

 Score =  110 bits (275), Expect = 4e-24
 Identities = 64/175 (36%), Positives = 98/175 (56%)
 Frame = +2

Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
           GS+KD +     +  +   K++++ILEG+ ++HS  I+HRDIK  N+L D    +KL DF
Sbjct: 442 GSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMILHRDIKGANILRDSTGNVKLGDF 501

Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
           G SK   L++ C S T   +  GTP +++PE+  G+ Y   ADIWS   +V+ M+T K P
Sbjct: 502 GASK--RLQTICLSGTGMKSVTGTPYWMSPEVISGQGYGRKADIWSVACTVVEMLTEKPP 559

Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
           +      A +FKI T       P   +S+  + F+  +   E   RPSA +LL+H
Sbjct: 560 WAEFEAMAAIFKIATQPTNPKLP-PHVSDYTRDFLKRIFV-EAKLRPSADELLRH 612
>sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32
          Length = 508

 Score =  110 bits (274), Expect = 5e-24
 Identities = 60/181 (33%), Positives = 100/181 (55%), Gaps = 9/181 (4%)
 Frame = +2

Query: 152 KDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDFGLS 331
           K+L+++   I    + K ++ +L GL ++H + +IHRDIKP+N+LL+ N  +KL DFG+S
Sbjct: 312 KNLLERGGRISEKVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILLNENGQVKLCDFGVS 371

Query: 332 KSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTPYHS 511
                       +   TF GT  Y+APE   G+PY   +D+WS G++++ +  GK P  S
Sbjct: 372 GE-------AVNSLATTFTGTSFYMAPERIQGQPYSVTSDVWSLGLTILEVANGKFPCSS 424

Query: 512 -------LPIPAVMFKIGTSKKIEFTPLEPI--SETMQSFIDYLLTPEQSERPSAKDLLK 664
                   P   +M+ +  + +++  P   I  S + +SFIDY L  +  ERPS + ++ 
Sbjct: 425 EKMAANIAPFELLMWILTFTPELKDEPESNIIWSPSFKSFIDYCLKKDSRERPSPRQMIN 484

Query: 665 H 667
           H
Sbjct: 485 H 485
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,296,415
Number of Sequences: 369166
Number of extensions: 1662566
Number of successful extensions: 7166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5568
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)