Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_M05
(765 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 116 8e-26
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 116 8e-26
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 114 4e-25
sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4 113 6e-25
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 113 6e-25
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 112 8e-25
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 112 1e-24
sp|P32491|MKK2_YEAST MAP kinase kinase MKK2/SSP33 112 1e-24
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 110 4e-24
sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32 110 5e-24
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 116 bits (290), Expect = 8e-26
Identities = 69/179 (38%), Positives = 101/179 (56%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+KD + + S K++++ILEG+ ++HS I+HRDIK N+L D +KL DF
Sbjct: 449 GSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDF 508
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK L++ C S T + GTP +++PE+ GE Y AD+WS G +V+ M+T K P
Sbjct: 509 GASK--RLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPP 566
Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKHSIFQ 679
+ A +FKI T P ISE + F+ + E +RPSA++LL H Q
Sbjct: 567 WAEYEAMAAIFKIATQPTNPQLP-SHISEHGRDFLRRIFV-EARQRPSAEELLTHHFAQ 623
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 116 bits (290), Expect = 8e-26
Identities = 69/179 (38%), Positives = 101/179 (56%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+KD + + S K++++ILEG+ ++HS I+HRDIK N+L D +KL DF
Sbjct: 449 GSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDF 508
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK L++ C S T + GTP +++PE+ GE Y AD+WS G +V+ M+T K P
Sbjct: 509 GASK--RLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPP 566
Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKHSIFQ 679
+ A +FKI T P ISE + F+ + E +RPSA++LL H Q
Sbjct: 567 WAEYEAMAAIFKIATQPTNPQLP-SHISEHGRDFLRRIFV-EARQRPSAEELLTHHFAQ 623
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 114 bits (284), Expect = 4e-25
Identities = 67/176 (38%), Positives = 101/176 (57%), Gaps = 1/176 (0%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+ L++K S V ++ ++L GLE++H+ I+HRDIK N+L+D+ +KL DF
Sbjct: 156 GSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADF 215
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK + + + + + GTP ++APE+ L + F ADIWS G +VI M+TGK P
Sbjct: 216 GASKQV---AELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAP 272
Query: 503 Y-HSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
+ A +F IGT+K P + IS F+ L E + RP+A +LLKH
Sbjct: 273 WSQQYKEIAAIFHIGTTKSHPPIP-DNISSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4
Length = 1142
Score = 113 bits (282), Expect = 6e-25
Identities = 64/185 (34%), Positives = 105/185 (56%), Gaps = 4/185 (2%)
Frame = +2
Query: 140 QGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLID 319
+G L +L+ + + + I+F ++I+ G+ + H+ GI+HRD+KPEN+LLDH +K+ D
Sbjct: 115 KGELFNLLVERGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHKYNIKIAD 174
Query: 320 FGLSKSLHLKSFCESTTKTV-TFCGTPNYLAPEIFLGEPYY-FPADIWSTGVSVIHMITG 493
FG++ E+ K + T CG+P+Y APEI G PY F +D+WS GV + ++TG
Sbjct: 175 FGMAA-------LETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTG 227
Query: 494 KTPYHSLP--IPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
+ P+ I ++ K+ + E + IS Q I +LT + R +D+LKH
Sbjct: 228 RLPFDEEDGNIRTLLLKV-QKGEFEMPSDDEISREAQDLIRKILTVDPERRIKTRDILKH 286
Query: 668 SIFQK 682
+ QK
Sbjct: 287 PLLQK 291
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 113 bits (282), Expect = 6e-25
Identities = 65/175 (37%), Positives = 99/175 (56%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+KD + + + K++++ILEG+ ++HS I+HRDIK N+L D +KL DF
Sbjct: 443 GSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDF 502
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK L++ C S T + GTP +++PE+ GE Y ADIWS +V+ M+T K P
Sbjct: 503 GASK--RLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPP 560
Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
+ A +FKI T P +S+ + F+ + E RPSA++LL+H
Sbjct: 561 WAEFEAMAAIFKIATQPTNPKLP-PHVSDYTRDFLKRIFV-EAKLRPSAEELLRH 613
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 112 bits (281), Expect = 8e-25
Identities = 66/176 (37%), Positives = 102/176 (57%), Gaps = 1/176 (0%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+ L++K S V +++++L GLE++H+ I+HRDIK N+L+D+ +KL DF
Sbjct: 157 GSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADF 216
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK + + + T + GTP ++APE+ L + F ADIWS G +VI M+TGK P
Sbjct: 217 GASKQV---AELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAP 273
Query: 503 Y-HSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
+ A +F IGT+K P + +S + F+ L + RP+A +LLKH
Sbjct: 274 WSQQYKEVAAIFFIGTTKSHPPIP-DTLSSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 112 bits (280), Expect = 1e-24
Identities = 69/183 (37%), Positives = 106/183 (57%), Gaps = 6/183 (3%)
Frame = +2
Query: 137 SQGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLI 316
+QGSL+ L +++ + S V ++++IL+GL+++H +G IHR+IK N+L+D N T+KL
Sbjct: 1708 TQGSLRKLYQRNQ-LGDSVVSLYTRQILDGLKYLHDKGFIHRNIKCANVLVDANGTVKLA 1766
Query: 317 DFGLSKSLHLKSFCESTTKTVTFCGTP--NYLAPEIFLG----EPYYFPADIWSTGVSVI 478
DFGL+K + L TP N++APE+ L + Y PADIWS G +V+
Sbjct: 1767 DFGLAKVMSL-------------WRTPYWNWMAPEVILNPKDYDGYGTPADIWSLGCTVL 1813
Query: 479 HMITGKTPYHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDL 658
M+TG+ PY L I ++ IGT K + + +S + FI L ERP+A +L
Sbjct: 1814 EMLTGQIPYSDLEIGTALYNIGTGKLPKIPDI--LSLDARDFILTCLKVNPEERPTAAEL 1871
Query: 659 LKH 667
L H
Sbjct: 1872 LNH 1874
>sp|P32491|MKK2_YEAST MAP kinase kinase MKK2/SSP33
Length = 506
Score = 112 bits (279), Expect = 1e-24
Identities = 62/185 (33%), Positives = 102/185 (55%), Gaps = 9/185 (4%)
Frame = +2
Query: 140 QGSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLID 319
+ + K+L+ + I + K ++ +L GL ++H + +IHRDIKP+N+LL+ +KL D
Sbjct: 301 EATYKNLLKRGGRISERVIGKIAESVLRGLSYLHERKVIHRDIKPQNILLNEKGEIKLCD 360
Query: 320 FGLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKT 499
FG+S + +TF GT Y+APE G+PY D+WS G++++ + G+
Sbjct: 361 FGVSGE-------AVNSLAMTFTGTSFYMAPERIQGQPYSVTCDVWSLGLTLLEVAGGRF 413
Query: 500 PYHS-------LPIPAVMFKIGTSKKIEFTPLEPI--SETMQSFIDYLLTPEQSERPSAK 652
P+ S PI + + S +++ P I S+T +SFIDY L + ERPS +
Sbjct: 414 PFESDKITQNVAPIELLTMILTFSPQLKDEPELDISWSKTFRSFIDYCLKKDARERPSPR 473
Query: 653 DLLKH 667
+LKH
Sbjct: 474 QMLKH 478
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 110 bits (275), Expect = 4e-24
Identities = 64/175 (36%), Positives = 98/175 (56%)
Frame = +2
Query: 143 GSLKDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDF 322
GS+KD + + + K++++ILEG+ ++HS I+HRDIK N+L D +KL DF
Sbjct: 442 GSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMILHRDIKGANILRDSTGNVKLGDF 501
Query: 323 GLSKSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTP 502
G SK L++ C S T + GTP +++PE+ G+ Y ADIWS +V+ M+T K P
Sbjct: 502 GASK--RLQTICLSGTGMKSVTGTPYWMSPEVISGQGYGRKADIWSVACTVVEMLTEKPP 559
Query: 503 YHSLPIPAVMFKIGTSKKIEFTPLEPISETMQSFIDYLLTPEQSERPSAKDLLKH 667
+ A +FKI T P +S+ + F+ + E RPSA +LL+H
Sbjct: 560 WAEFEAMAAIFKIATQPTNPKLP-PHVSDYTRDFLKRIFV-EAKLRPSADELLRH 612
>sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32
Length = 508
Score = 110 bits (274), Expect = 5e-24
Identities = 60/181 (33%), Positives = 100/181 (55%), Gaps = 9/181 (4%)
Frame = +2
Query: 152 KDLIDKSKWIKHSKVIKFSKEILEGLEFIHSQGIIHRDIKPENMLLDHNETLKLIDFGLS 331
K+L+++ I + K ++ +L GL ++H + +IHRDIKP+N+LL+ N +KL DFG+S
Sbjct: 312 KNLLERGGRISEKVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILLNENGQVKLCDFGVS 371
Query: 332 KSLHLKSFCESTTKTVTFCGTPNYLAPEIFLGEPYYFPADIWSTGVSVIHMITGKTPYHS 511
+ TF GT Y+APE G+PY +D+WS G++++ + GK P S
Sbjct: 372 GE-------AVNSLATTFTGTSFYMAPERIQGQPYSVTSDVWSLGLTILEVANGKFPCSS 424
Query: 512 -------LPIPAVMFKIGTSKKIEFTPLEPI--SETMQSFIDYLLTPEQSERPSAKDLLK 664
P +M+ + + +++ P I S + +SFIDY L + ERPS + ++
Sbjct: 425 EKMAANIAPFELLMWILTFTPELKDEPESNIIWSPSFKSFIDYCLKKDSRERPSPRQMIN 484
Query: 665 H 667
H
Sbjct: 485 H 485
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,296,415
Number of Sequences: 369166
Number of extensions: 1662566
Number of successful extensions: 7166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5568
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)