Planaria EST Database


DrC_02276

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02276
         (402 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q15388|TOM20_HUMAN  Mitochondrial import receptor subunit...   106   2e-23
sp|Q9DCC8|TOM20_MOUSE  Mitochondrial import receptor subunit...   105   3e-23
sp|Q62760|TOM20_RAT  Mitochondrial import receptor subunit T...   103   1e-22
sp|Q19766|TOM20_CAEEL  Mitochondrial import receptor subunit...    77   2e-14
sp|P35848|TOM20_NEUCR  Mitochondrial import receptor subunit...    34   0.12 
sp|O14225|TOM20_SCHPO  Mitochondrial import receptor subunit...    33   0.16 
sp|Q6KAR6|EXOC3_MOUSE  Exocyst complex component 3 (Exocyst ...    30   2.3  
sp|Q62825|EXOC3_RAT  Exocyst complex component 3 (Exocyst co...    30   2.3  
sp|Q896M5|SYE_CLOTE  Glutamyl-tRNA synthetase (Glutamate--tR...    28   5.2  
sp|Q8VCF5|TSN10_MOUSE  Tetraspanin-10 (Tspan-10) (Oculospanin)     28   6.8  
>sp|Q15388|TOM20_HUMAN Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
           20 kDa outer membrane protein) (Outer mitochondrial
           membrane receptor Tom20)
          Length = 145

 Score =  106 bits (264), Expect = 2e-23
 Identities = 53/127 (41%), Positives = 78/127 (61%), Gaps = 3/127 (2%)
 Frame = +1

Query: 19  ALGAGVA---FISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVAR 189
           A+ AGV    FI YCIYFDRKRRS P+F              K +  +  LPD+ +  A 
Sbjct: 7   AIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAV 66

Query: 190 QEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTL 369
           Q+FFLE+I+ G++ ++ G +E+G+  L  A++VC  PQ LL+V +QTL P  + MLLT L
Sbjct: 67  QKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKL 126

Query: 370 PKMKEQV 390
           P + +++
Sbjct: 127 PTISQRI 133
>sp|Q9DCC8|TOM20_MOUSE Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
           20 kDa outer membrane protein) (Outer mitochondrial
           membrane receptor Tom20)
          Length = 145

 Score =  105 bits (263), Expect = 3e-23
 Identities = 53/127 (41%), Positives = 78/127 (61%), Gaps = 3/127 (2%)
 Frame = +1

Query: 19  ALGAGVA---FISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVAR 189
           A+ AGV    FI YCIYFDRKRRS P+F              K +  +  LPD+ +  A 
Sbjct: 7   AIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAV 66

Query: 190 QEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTL 369
           Q+FFLE+I+ G++ ++ G +E+G+  L  A++VC  PQ LL+V +QTL P  + MLLT L
Sbjct: 67  QKFFLEEIQLGEELLAQGDYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKL 126

Query: 370 PKMKEQV 390
           P + +++
Sbjct: 127 PTISQRI 133
>sp|Q62760|TOM20_RAT Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
           20 kDa outer membrane protein) (Outer mitochondrial
           membrane receptor Tom20)
          Length = 145

 Score =  103 bits (258), Expect = 1e-22
 Identities = 49/120 (40%), Positives = 73/120 (60%)
 Frame = +1

Query: 31  GVAFISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVARQEFFLEQ 210
           G  FI YCIYFDRKRR  P+F              K +  +  LPD+ +  A Q+FFLE+
Sbjct: 14  GALFIGYCIYFDRKRRGDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEE 73

Query: 211 IRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTLPKMKEQV 390
           I+ G++ ++ G +E+G+  L  A++VC  PQ LL+V +QTL P  + MLLT LP + +++
Sbjct: 74  IQLGEELLAQGDYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRI 133
>sp|Q19766|TOM20_CAEEL Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
           20 kDa outer membrane protein) (Outer mitochondrial
           membrane receptor TOM20)
          Length = 188

 Score = 76.6 bits (187), Expect = 2e-14
 Identities = 43/137 (31%), Positives = 71/137 (51%), Gaps = 8/137 (5%)
 Frame = +1

Query: 13  IVALG-AGVAFISYCIYFDRKRRSHPDFXXXXXXXXXXXXD-NKMKIKIPTL------PD 168
           ++A G AG AF+ YCIYFD KR + PD+                M  + P        PD
Sbjct: 14  VLAAGIAGAAFLGYCIYFDHKRINAPDYKDKIRQKRRAQAGAGGMAPRRPAAAGNDAAPD 73

Query: 169 MNNNVARQEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAY 348
           + +    Q FFL++++ G++ M+ G  +EG   +A A+ +C   Q LL +F+QTL    +
Sbjct: 74  VTDPSQMQRFFLQEVQLGEELMAAGNVDEGAVHIANAVMLCGESQQLLSIFQQTLSEDQF 133

Query: 349 DMLLTTLPKMKEQVDEL 399
             ++  LP  +E++ E+
Sbjct: 134 RAVVQQLPSTRERLAEM 150
>sp|P35848|TOM20_NEUCR Mitochondrial import receptor subunit tom-20 (Mitochondrial 20 kDa
           outer membrane protein) (Translocase of outer membrane
           20 kDa subunit) (MOM19 protein)
          Length = 181

 Score = 33.9 bits (76), Expect = 0.12
 Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 26/142 (18%)
 Frame = +1

Query: 10  YIVALGAGVA--FISYCIYFDRKRRSHPDF----------------------XXXXXXXX 117
           Y  A  A VA  F++Y +YFD KRR+ P+F                              
Sbjct: 8   YTTAAVAAVATGFLAYAVYFDYKRRNDPEFRRQLRRSARRQARQEKEYAELSQQAQRQRI 67

Query: 118 XXXXDNKMKIKIPTLPDMNNNVARQEFFLEQIRYGDQCMS--TGAFEEGIQRLALALSVC 291
               D   +   PT  D      ++ +FLEQ++ G+      T A +  +     AL V 
Sbjct: 68  RQMVDEAKEEGFPTTSD-----EKEAYFLEQVQAGEILGQDPTKAIDASL-AFYKALKVY 121

Query: 292 ANPQNLLKVFEQTLQPQAYDML 357
             P +L+ ++++T+     D+L
Sbjct: 122 PTPGDLISIYDKTVAKPILDIL 143
>sp|O14225|TOM20_SCHPO Mitochondrial import receptor subunit tom20 (Mitochondrial 20 kDa
           outer membrane protein) (Translocase of outer membrane
           20 kDa subunit)
          Length = 152

 Score = 33.5 bits (75), Expect = 0.16
 Identities = 27/126 (21%), Positives = 49/126 (38%), Gaps = 19/126 (15%)
 Frame = +1

Query: 37  AFISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIK-----------------IPTLP 165
           A + Y IYFD KRR+ P F            + K + +                 + + P
Sbjct: 14  AAVGYAIYFDYKRRNDPHFRKTLKRRYKKVHEAKKQEEKLATKKFDITVEEALQVVASTP 73

Query: 166 DMNNNVARQEFFLEQIRYGDQC--MSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQP 339
             ++   ++ FF++Q+  G+Q         +E       AL V   P  L  ++E+T+  
Sbjct: 74  VPSSAEEKELFFMQQVARGEQLFQQQPDNIKESAACFYSALKVYPQPVELFAIYERTVPE 133

Query: 340 QAYDML 357
              ++L
Sbjct: 134 PIMNLL 139
>sp|Q6KAR6|EXOC3_MOUSE Exocyst complex component 3 (Exocyst complex component Sec6)
          Length = 755

 Score = 29.6 bits (65), Expect = 2.3
 Identities = 19/60 (31%), Positives = 29/60 (48%)
 Frame = -3

Query: 352 YRMPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQLTCIDHRNESVPKRILVLLHYY 173
           + +PE+V+  Q L E    L  H KL    +    +  L C  +R +S  KR + L+H Y
Sbjct: 130 FSVPEIVRETQDLIEQGALLQAHRKL---MDLECSRDGLMCEQYRMDSGNKRDMTLIHGY 186
>sp|Q62825|EXOC3_RAT Exocyst complex component 3 (Exocyst complex component Sec6)
           (rSec6)
          Length = 755

 Score = 29.6 bits (65), Expect = 2.3
 Identities = 19/60 (31%), Positives = 29/60 (48%)
 Frame = -3

Query: 352 YRMPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQLTCIDHRNESVPKRILVLLHYY 173
           + +PE+V+  Q L E    L  H KL    +    +  L C  +R +S  KR + L+H Y
Sbjct: 130 FSVPEIVRETQDLIEQGALLQAHRKL---MDLECSRDGLMCEQYRMDSGNKRDMTLIHGY 186
>sp|Q896M5|SYE_CLOTE Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
          Length = 488

 Score = 28.5 bits (62), Expect = 5.2
 Identities = 21/61 (34%), Positives = 33/61 (54%), Gaps = 6/61 (9%)
 Frame = -3

Query: 346 MPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQ-----LTCIDHRN-ESVPKRILVL 185
           +PE V F  +L E   DL++H K+K  FE  L+  +     L  ID+ N E++   I+ L
Sbjct: 371 IPEQVDFFDELPEYSCDLYVHKKMKTNFENSLESLEKALPVLESIDNWNTETIHDSIMEL 430

Query: 184 L 182
           +
Sbjct: 431 I 431
>sp|Q8VCF5|TSN10_MOUSE Tetraspanin-10 (Tspan-10) (Oculospanin)
          Length = 331

 Score = 28.1 bits (61), Expect = 6.8
 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 12/48 (25%)
 Frame = +1

Query: 196 FFLEQIRYGDQCMSTGAFEE------------GIQRLALALSVCANPQ 303
           F L+Q++ G QC    ++++            G+Q  +L  S C NPQ
Sbjct: 199 FLLDQVQLGLQCCGAVSYQDWQQNLYFNCSSPGVQACSLPASCCINPQ 246
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,012,886
Number of Sequences: 369166
Number of extensions: 494380
Number of successful extensions: 1269
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1265
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1758783250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)